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A five-coordinate diorganotin(IV) complex [PhSn(L)]·DMF and its ligand, 5-hydroxy-3-methyl-5-phenyl-( N-phenylthiosemicarbazone)pyrazoline (HL), were synthesized and characterized by elemental analysis, IR, NMR (H, C) spectroscopies and X-ray crystallography techniques. Our studies revealed that HL has formed as 5-hydroxy-4,5-dihydropyrazoline derivative in orthorhombic, Pbca space group with a = 15.4330(3) Å, b = 11.5427(2) Å, c = 18.1440(3) Å whereas [PhSn(L)]·DMF crystallizes with two independent molecules ( a and b) in the asymmetric unit in triclinic system, Pī space group with a = 9.2795(1) Å, b = 15.8315(2) Å, c = 19.9895(3) Å, α = 100.855(1)°, β = 92.985(1)°, γ = 102.243(1)°. In molecule a, the central Sn(IV) atom adopts a distorted tetragonal-pyramidal (TTP) geometry with N2 atom at apical position, while in molecule b the Sn(IV) occupies the centre of a trigonal-bipyramid (TBP) with N2 atom of the ONS-tridentate ligand and both Ph groups occupying equatorial positions. One molecule of DMF (crystallization solvent) helps in stabilizing the crystal structure. Graphical Abstract: [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

• LQFM219 acts as a free radical scavenger, reducing MPO, TNF-α, and IL-1β levels. LQFM219 is a molecule designed from celecoxibe (COX-2 inhibitor) and darbufelone (inhibitor of COX-2 and 5-LOX) lead compounds through a molecular hybridisation strategy. Therefore, this work aimed to investigate the antinociceptive and anti-inflammatory activities of this new hybrid compound. The acute oral systemic toxicity of LQFM219 was evaluated via the neutral red uptake assay. Acetic acid-induced abdominal writhing and CFA-induced mechanical hyperalgesia were performed to evaluate the antinociceptive activity, and the anti-oedematogenic activity was studied by CFA-induced paw oedema and croton oil-induced ear oedema. Moreover, the acute anti-inflammatory activity was determined by carrageenan-induced pleurisy. In addition, cell migration, myeloperoxidase enzyme activity, and TNF-α and IL-1β levels were determined in pleural exudate. Moreover, a redox assay was conducted using electroanalytical and DPPH methods. The results demonstrated that LQFM219 was classified as GHS category 4, and it showed better free radical scavenger activity compared to BHT. Besides, LQFM219 decreased the number of writhings induced by acetic acid and the response to the mechanical stimulus in the CFA-induced mechanical hyperalgesia test. Furthermore, LQFM219 reduced oedema formation, cell migration, and IL-1β and TNF-α levels in the pleural cavity and inhibited myeloperoxidase enzyme activity. Thus, our study provides that the new pyrazole derivative, LQFM219, demonstrated low toxicity, antinociceptive and anti-inflammatory potential in vitro and in vivo. [ABSTRACT FROM AUTHOR]