1. The role of wogonin in controlling SOCS3 expression in neuronal cells.
- Author
-
Nam KW, Chae S, Song HY, Mar W, and Han MD
- Subjects
- Animals, Cell Line, Tumor, Humans, Male, Neurons metabolism, Rats, Rats, Sprague-Dawley, Suppressor of Cytokine Signaling 3 Protein, Flavanones pharmacology, Gene Expression Regulation drug effects, Neurons drug effects, Suppressor of Cytokine Signaling Proteins genetics
- Abstract
The mechanism underlying the wogonin-mediated increase in the expression of suppressor of cytokine signaling 3 (SOCS3) is unclear. Promoter deletion assay results revealed that wogonin-induced SOCS3 expression is dependent on the AP-1 consensus sequences and two STAT responsive elements (TTACAAGAA and TTCCAGGAA) in the 5'-flanking region of the SOCS3 gene in SH-SY5Y cells. Wogonin-induced SOCS3 expression was blocked by inhibitors of PI3K, Akt, Raf, p38, JNK, MEK, and STAT3, respectively. However, JAK2 inhibitors did not inhibit wogonin-induced SOCS3 expression. These results indicate that SOCS3-inducing effect of wogonin is caused by the activation of PI3K-mediated MAPK signaling pathways (Akt, ERK1/2, p38, and JNK), and the subsequent activation of AP-1 consensus sequences and STAT responsive elements in SH-SY5Y cells., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF