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BACKGROUND: As a degradable scaffold material for bone tissue engineering, lactic acid is widely used in tissue regeneration and repair research, and plays an important role in promoting tissue healing, new bone formation and angiogenesis. OBJECTIVE: To observe the effect of lactic acid degradation products on osteoclasts and to investigate the effects of lactic-interfered osteoclast conditioned medium on the proliferation, migration and tube-forming capacity of human umbilical vein endothelial cells. METHODS: (1) The mouse monocyte macrophage cell line RAW264.7 at logarithmic growth period was selected, and adherent cells were cultured in the osteoclast induction medium (DMEM medium with nuclear factor-κB receptor-activating factor ligand and 10% fetal bovine serum) containing different concentrations of lactic acid (0, 5, 10, 20 mmol/L). After 5 days of culture, tartrate-resistant acid phosphatase staining and cytoskeletal fibrillar actin staining were conducted. After 24 hours of culture, RT-PCR was used to detect the mRNA expression of tartrate-resistant acid phosphatase 5. (2) RAW264.7 cells at logarithmic growth period were selected and adherent cells were divided into two groups. Control group was cultured in the osteoclast induction medium, while experimental group was cultured in the osteoclast induction medium containing 10 mmol/L lactic acid. After 5 days of culture, the medium in each group was removed and the cells in the two groups were cultured in the serum-free DMEM medium for another 24 hours. Cell supernatant was then collected and used as the conditioned medium after mixed with an equal volume of DMEM medium containing 10% fetal bovine serum. Human umbilical vein endothelial cells at the logarithmic growth phase were taken and separately co-cultured with the conditioned medium of the control and experimental groups. The proliferation, migration and tube-forming ability of human umbilical vein endothelial cells were observed by cell counting kit-8 assay, migration assay, scratch assay and tube-forming assay. The mRNA and protein expression of angiogenesis-related genes and proteins were observed by RT-PCR and western blot. RESULTS AND CONCLUSION: Tartrate-resistant acid phosphatase staining and cytoskeletal fibrillar actin staining showed that 5 and 10 mmol/L lactic acid promoted osteoclastic differentiation of RAW264.7 cells and the promoting effect of 10 mmol/L lactate was more significant. RT-PCR results showed that the expression of tartrate-resistant acid phosphatase-5 mRNA of osteoclast-related genes was the highest when the lactic acid concentration was 5, 10, and 20 mmol/L (P < 0.05), especially 10 mmol/L. Compared with the control group, the proliferation, migration and tube-forming abilities of human umbilical vein endothelial cells were significantly increased in the experimental group (P < 0.05). Compared with the control group, the expression levels of vascular endothelial growth factor and angiogenin 1 mRNA and protein were increased in the experimental group (P < 0.05). To conclude, lactate-induced osteoclast conditioned medium could promote the angiogenesis of endothelial cells, and the mechanism may be related to the promotion of the expression of vascular endothelial growth factor and angiogenin 1. [ABSTRACT FROM AUTHOR]

BACKGROUND: Three-dimensional (3D) printing is an emerging technology in the field of dentistry. It utilizes a layer-by-layer manufacturing technique to create scaffolds suitable for periodontal tissue engineering applications. Tissue scaffolds produced through 3D printing can possess controlled characteristics, including internal structure, porosity, and interconnectivity, making it an ideal strategy for periodontal tissue engineering. OBJECTIVE: To review the applications of 3D printed scaffolds in periodontal regeneration. METHODS: English search terms were “3D printing, periodontal tissue engineering, additive manufacturing, regenerative medicine, bioengineering, scaffold, bioprinting, periodontitis”. Chinese search terms were “3D printing, additive manufacturing, periodontal tissue engineering, scaffolds, bio-inks, bioprinting, tissue engineering”. Relevant literature published from 2000 to 2023 in PubMed and CNKI databases was retrieved and included in the review. RESULTS AND CONCLUSION: Over the past few decades, 3D printing technology has made significant progress and breakthroughs in tissue engineering and biomedical fields. 3D printing technology can provide highly personalized treatment programs, improve the suitability and therapeutic effect of therapeutic stents, and has broad application prospects in periodontal tissue engineering. In periodontal tissue engineering, 3D printing applications can better mimic the complex structures of biological tissues and manufacture biocompatible scaffold materials with suitable mechanical and rheological properties. The layer-bylayer construction of tissue engineering scaffolds through 3D printing not only enables the creation of precise and intricate scaffold models for personalized treatment of periodontal disease but also facilitates the incorporation of complex microstructures and channels within the scaffolds to promote cell growth and tissue regeneration. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Natural Disasters is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Geological Hazard & Control is the property of China Institute of Geological Environmental Monitoring (CIGEM) Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Engineering Geology / Gongcheng Dizhi Xuebao is the property of Journal of Engineering Geology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Engineering Geology / Gongcheng Dizhi Xuebao is the property of Journal of Engineering Geology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)