A retrospective study was conducted among out-patient and in-patient in Kathmandu medical college Teaching hospital of one month from the duration 2060-11-15 to 2060-12 -15. Mid-stream urine [MSU] of 300 patient were cultured. Out of these, 75 patients’ urine were found to have significant bacterial growth. Total of five species of bacterial species isolated were viz.E.coli 37(33.3%), Proteus species 25(27.7%), Klebsiella species 15(16.6%), Staphylococcus aureus 8(8.8%) and Pseudomonas aureginosa 1(1.1%). Regarding their antibiotic sensitivity pattern, E. coli were sensitive to Nitrofurantoin 31 (83.8% ), Norfloxacin 25 (67.5%), Ofloxacin 30(81.0%), Amoxycillin 16(43.2%), Nalidixic acid 15 (40.5%).proteus species were sensitive to Nitrofurantoin 15 (60.0%), Norfloxacin 15 (60.0%), Ofloxacin 12(48.0%), Amoxycillin 13(52.0%), Nalidixic acid 10 (40.0%), Ciprofloxacillin 12(48.0%). Klebsiella species were sensitive to Nitrofurantoin 9 (60.0%), Norfloxacin 5 (33.3%), Oflaxin 6(40.0%), Amoxycillin 5(33.3%), Nalidixic acid 4 (26.6%), ciprofloxacillin 6(40.0%). Staphylloccus aureus were sensitive to Nitrofurantoin 8(100.0%), Norfloxacin 6(75.0%), Oflaxin 8(100.0%), Amoxycillin 7(87.5%), Nalidixic acid 6(75.0%), Ciprofloxacillin 8(100 %). And Pseudomonas aureginosa were resistant to all antibiotics which we are using in this research work. Key Words: Urine sample, Identification, Sensitivity pattern.