1. Raman microspectroscopy detects epigenetic modifications in living Jurkat leukemic cells
- Author
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Teddy Happillon, Aurelie Trussardi-Regnier, Philippe Bernard, Michel Manfait, Olivier Piot, Frank Antonicelli, Mathilde Poplineau, Jean Dufer, Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Cancer Research ,Optical Tweezers ,Pyridines ,Hydroxamic Acids ,Spectrum Analysis, Raman ,01 natural sciences ,Jurkat cells ,Epigenesis, Genetic ,Histones ,03 medical and health sciences ,symbols.namesake ,Jurkat Cells ,Genetics ,medicine ,Cluster Analysis ,Humans ,Epigenetics ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,Image Cytometry ,0303 health sciences ,biology ,010401 analytical chemistry ,Acetylation ,Chromatin ,0104 chemical sciences ,Cell biology ,Histone Deacetylase Inhibitors ,Histone ,Trichostatin A ,Biochemistry ,Benzamides ,biology.protein ,symbols ,Electrophoresis, Polyacrylamide Gel ,Histone deacetylase ,Raman spectroscopy ,medicine.drug - Abstract
Aims: Classical biochemical and molecular methods for discerning cells with epigenetic modifications are often biologically perturbing or even destructive. We wondered whether the noninvasive laser tweezer Raman spectroscopy technique allowed the discrimination of single living human cells undergoing epigenetic modifications. Materials & methods: Human Jurkat leukemic cells were treated with inhibitors of histone deacetylases (trichostatin A and MS-275). Epigenetic changes were monitored through histone electrophoresis, nuclear image cytometry and laser tweezer Raman spectroscopy. Results: Treatment of Jurkat cells with histone deacetylase inhibitors increased histone acetylation and induced chromatin organization changes. Characteristic vibrations, issued from laser tweezer Raman spectroscopy analyses, mostly assigned to DNA and proteins allowed discerning histone deacetylase inhibitor-treated cells from control with high confidence. Statistical processing of laser tweezer Raman spectroscopy data led to the definition of specific biomolecular fingerprints of each cell group. Conclusion: This original study shows that laser tweezer Raman spectroscopy is a label-free rapid tool to identify living cells that underwent epigenetic changes.
- Published
- 2011