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Low‐cost automated histopathology microscopy systems usually suffer from optical imperfections, producing images that are slightly Out of Focus (OoF). In this work, a guided filter (GF) based image preprocessing is proposed for compensating focal errors and its efficacy is demonstrated on images of healthy and malaria infected red blood cells (h‐RBCs and i‐RBCs), and PAP smears. Since contrast enhancement has been widely used as an image preprocessing step for the analysis of histopathology images, a systematic comparison is made with six such prominently used methods, namely Contrast Limited Adaptive Histogram Equalization (CLAHE), RIQMC‐based optimal histogram matching (ROHIM), modified L0, Morphological Varying(MV)‐Bitonic filter, unsharp mask filter and joint bilateral filter. The images enhanced using GF approach lead to better segmentation accuracy (upto 50% improvement over native images) and visual quality compared to other approaches, without any change in the color tones. Thus, the proposed GF approach is a viable solution for rectifying the OoF microscopy images without the loss of the valuable diagnostic information presented by the color tone. [ABSTRACT FROM AUTHOR]

We report on highly accurate femtosecond (fs) laser micromachining of a compound parabolic concentrator (CPC) fiber tip on a polymer optical fiber (pOf). The accuracy is reflected in an unprecedented correspondence between the numerically predicted and experimentally found improvement in fluorescence pickup efficiency of a Förster resonance energy transfer-based POF glucose sensor. A Zemax model of the CPC-tipped sensor predicts an optimal improvement of a factor of 3.96 compared to the sensor with a plane-cut fiber tip. The fs laser micromachined CPC tip showed an increase of a factor of 3.5, which is only 11.6% from the predicted value. Earlier state-of-the-art fabrication of the CPC-shaped tip by fiber tapering was of so poor quality that the actual improvement was 43% lower than the predicted improvement of the ideal CPC shape. [ABSTRACT FROM AUTHOR]

We report on a simple chemical etching method that enables nonlinear tapering of Polymer Optical Fiber (POF) tips to manufacture Compound Parabolic Concentrator (CPC) fiber tips. We show that, counter-intuitively, nonlinear tapering can be achieved by first etching the core and not the cladding. The etching mechanism is modelled and etched tips are characterized both geometrically and optically in a fluorescence glucose sensor chemistry. A Zemax model of the CPC tipped sensor predicts an optimal improvement in light capturing efficiency of a factor of 3.96 compared to the conventional sensor with a plane-cut fiber tip. A batch of eight CPC fiber tips has been manufactured by the chemical etching method. The batch average showed an increase of a factor of 3.16, which is only 20% less than the predicted value. The method is reproducible and can be up-scaled for mass production.

We report on a simple chemical etching method that enables nonlinear tapering of Polymer Optical Fiber (POF) tips to manufacture Compound Parabolic Concentrator (CPC) fiber tips. We show that, counter-intuitively, nonlinear tapering can be achieved by first etching the core and not the cladding. The etching mechanism is modelled and etched tips are characterized both geometrically and optically in a fluorescence glucose sensor chemistry. A Zemax model of the CPC tipped sensor predicts an optimal improvement in light capturing efficiency of a factor of 3.96 compared to the conventional sensor with a plane-cut fiber tip. A batch of eight CPC fiber tips has been manufactured by the chemical etching method. The batch average showed an increase of a factor of 3.16, which is only 20% less than the predicted value. The method is reproducible and can be up-scaled for mass production. [ABSTRACT FROM AUTHOR]