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Next to available data about actual Internet use of young children at home, most research especially focuses on the threats and opportunities about active Internet usage. Limited empirical research focuses on the role and impact of parents in this context. In the present study, Internet parenting styles are defined and operationalized to study the impact on actual Internet usage of children at home. Two dimensions are distinguished in Internet parenting styles: parental control and parental warmth. Based on a survey, involving 533 parents from children in primary schools, this Internet usage was studied from the perspective of Internet parenting styles. Results point at high Internet access at home. As to the parenting styles, we observe a dominance of the authoritative parenting style (59.4%). The styles differ when controlling for parent gender, educational background and age. Parenting styles are also linked to level of parent Internet usage, Internet attitude and Internet experience. Parenting styles also significantly affect child Internet usage. The highest child usage level is perceived when parents adopt a permissive parenting style; the lowest level is observed when parents adopt an authoritarian Internet parenting style. The variables Internet parenting style, parent Internet behavior, and parent educational background significantly predict Internet usage of children at home (R[superscript 2] = 0.44). Theoretical and practical implications are discussed and directions for future research. (Contains 1 table and 4 figures.)

Peripheral blood T cells transduced with a tumor-specific T-cell receptor (TCR) face problems of auto-reactivity and lack of efficacy caused by cross-pairing of exogenous and endogenous TCR chains, as well as short term in vivo survival due to activation and growth factor-induced differentiation. We here studied an alternative strategy for the efficient generation of naive CD8+ T cells with a single TCR. TCR-transduced human postnatal thymus-derived and adult mobilized blood-derived hematopoietic progenitor cells (HPCs) were differentiated to CD4+CD8+ double-positive T cells using OP9-Delta-like 1 (OP9-DL1) cultures. Addition of the agonist peptide induced double positive cells to cross-present the peptide, leading, in the absence of co-stimulation, to cell cycle arrest and differentiation into mature CD8+ T cells. Comprehensive phenotypic, molecular and functional analysis revealed the generation of naive and resting CD8+ T cells through a process similar to thymic positive selection. These mature T cells show a near complete inhibition of endogenous TCRA and TCRB rearrangements and express high levels of the introduced multimer-reactive TCR. Upon activation, specific cytokine production and efficient killing of tumor cells were induced. Using this strategy, large numbers of high-avidity tumor-specific naive T cells can be generated from readily available HPCs without TCR chain cross-pairing. [ABSTRACT FROM AUTHOR]