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Emulsified polycolloid substrate (EPS) was developed and applied in situ to form a biobarrier for the containment and enhanced bioremediation of a petroleum-hydrocarbon plume. EPS had a negative zeta potential (−35.7 mv), which promoted its even distribution after injection. Batch and column experiments were performed to evaluate the effectiveness of EPS on toluene containment and biodegradation. The EPS-to-water partition coefficient for toluene (target compound) was 943. Thus, toluene had a significant sorption affinity to EPS, which caused reduced toluene concentration in water phase in the EPS/water system. Groundwater containing toluene (18 mg/L) was pumped into the three-column system at a flow rate of 0.28 mL/min, while EPS was injected into the second column to form a biobarrier. A significant reduction of toluene concentration to 0.1 mg/L was observed immediately after EPS injection. This indicates that EPS could effectively contain toluene plume and prevent its further migration to farther downgradient zone. Approximately 99% of toluene was removed after 296 PVs of operation via sorption, natural attenuation, and EPS-enhanced biodegradation. Increase in total organic carbon and bacteria were also observed after EPS supplement. Supplement of EPS resulted in a growth of petroleum-hydrocarbon degrading bacteria, which enhanced the toluene biodegradation.

Utilization of bacterial volatilization can be problematic to remediate mercury (Hg)-contaminated soils because most of the Hg in soils is bound to soil particles. The objective of this study was to develop a two-stage system (chemical extraction followed by microbial reduction) for Hg-contaminated soil remediation. The tasks were to (1) select the extraction reagents for Hg extraction, (2) assess the effects of extraction reagents on the growth of Hg-reducing bacterial strains, and (3) evaluate the effectiveness of Ca2+ and Mg2+ addition on merA gene (Hg reductase) induction. Bacterial inhibition was observed with the addition of 0.1 M ethylenediaminetetraacetic acid or citric acid. Up to 65% of Hg was biotransformed (Hg concentration = 69 mg/kg) from the soils after a 24 h extraction using 0.5 M ammonium thiosulfate. Ca2+ and Mg2+ were selected because they have the same electric charge as Hg and the studied groundwater contained high concentrations of Ca2+ and Mg2+. Results showed that the addition of 200 mg/L Ca2+ or 650 mg/L Mg2+ could reach effective merA induction. In the two-stage experiment, 120 mg/kg Hg-contaminated soils were extracted with 2 rounds of extraction processes for 10 h using 0.5 M ammonium thiosulfate. Approximately 77% of Hg was extracted from the soils after the first-step extraction process. Up to 81% of Hg2+ was transformed from the washing solution via the biotransformation processes with Enterobacter cloacae addition and Ca2+ and Mg2+ supplementation. The two-stage remedial system has the potential to be developed into a practical technology to remediate Hg-contaminated sites.

The Love River and Ho-Jin River, two major urban rivers in Kaohsiung City, Taiwan, are moderately to heavily polluted because different types of improperly treated wastewaters are discharged into the rivers. In this study, sediment and river water samples were collected from two rivers to investigate the river water quality and accumulation of polycyclic aromatic hydrocarbons (PAHs) in sediments. The spatial distribution, composition, and source appointment of PAHs of the sediments were examined. The impacts of PAHs on ecological system were assessed using toxic equivalence quotient (TEQ) of potentially carcinogenic PAHs (TEQcarc) and sediment quality guidelines. The average PAHs concentrations ranged from 2161 ng/g in Love River sediment to 160 ng/g in Ho-Jin River sediment. This could be due to the fact that Love River Basin had much higher population density and pyrolytic activities. High-ring PAHs (4-6 rings) contributed to 59-90% of the total PAHs concentrations. Benzo(a)pyrene (BaP) had the highest toxic equivalence quotient (up to 188 ng TEQ/g). Moreover, the downstream sediments contained higher TEQ of total TPHs than midstream and upstream sediment samples. The PAHs were adsorbed onto the fine particles with high organic content. Results from diagnostic ratio analyses indicate that the PAHs in two urban river sediments might originate from oil/coal combustion, traffic-related emissions, and waste combustion (pyrogenic activities). Future pollution prevention and management should target the various industries, incinerators, and transportation emission in this region to reduce the PAHs pollution.

Phytoremediation is an environmentally friendly green rehabilitation technology that is often incorporated with an application to improve phytohormones required for the growth of agricultural plants with the expectation to improve the effectiveness of plant rehabilitation. This study adopts phytoremediation, a green remediation technology, for the sake of restoring soil fertility and ensuring environmental sustainability, and adds ethylenediaminedisuccinic acid (EDDS) and the plant growth regulator (GA3) to examine the overall efficiency of phytoremediation. The experiments using pots in this study finds that environmentally sustainable phytoremediation achieves the greatest efficacy regarding the remediation of soil polluted by copper, zinc and nickel. The best combination of operational factors is the addition of the EDDS and GA3. The environment where the EDDS is added shows a poorer performance in the remediation of the heavy metal lead. In addition, the PCR(Polymerase chain reaction)-DGGE analysis results of bacterial flora change show that the combination “heavy metal + EDDS + GA3” brings about the richest bacterial flora, indicating that the addition of EDDS and GA3 can stimulate microbial growth, thereby achieving richer bacterial flora.

The goal of this study was to establish a modeling tool for river water quality with a direct linkage to the water quality index (WQI5) calculation and the river water quality model, the Water Quality Analysis Simulation Program (WASP), for pollutant transport modeling. The integrated WASP and WQI5 tool was field-tested to assess pollutant loadings and their impacts on river environment. Suspended solid (SS) and electric conductivity (EC) correlation equations and the WQI5 calculation tool were included in the water quality model and direct WQI5 calculation. The SS concentration, which was influenced by river flows, had crucial effects on river water quality and WQI5 values. EC value was controlled by dissolution of soil minerals, which was affected by the watershed drainage area and surface runoff. The integrated system could establish a direct correlation for river water quality, river flow, and WQI5.

Intrinsic bioremediation (IB) is the major mechanism causing the containment of petroleum hydrocarbons in groundwater. The effectiveness and mechanisms of IB on the contaminant removal were evaluated in this study. Groundwater was analyzed for the efficiency of IB, dominant bacteria, bacterial diversity, and biodegradation rates. Concentrations of benzene, toluene, ethylbenzene, and xylenes (BTEX) and methyl tert-butyl ether (MTBE) dropped from 6.5 and 0.14 mg/L in source area to 67 and 19 µg/L at the 145 m downgradient area. The biodegradation capacities for BTEX (50.9 mg/L) and MTBE (58.5 mg/L) were higher than the BTEX (6.5 mg/L) and MTBE (0.14 mg/L) concentrations within the plume. The bacterial diversities were assessed by 16S rRNA-based denatured gradient gel electrophoresis and nucleotide sequence analyses. More than 21 petroleum–hydrocarbon biodegrading bacterial species were detected in groundwater indicating that contaminants could be biodegraded by natural-occurring bacteria. The calculated MTBE and BTEX first-order decay rates were 5.7 × 10−4 and 1.3 × 10−3 per day, respectively. The high decay rates indicate that IB mechanisms played important roles in contaminant removal. The observed evidences of IB processes in highly contaminated areas included: (1) decreased electron acceptors, (2) production of degradation byproducts, (3) increased microbial populations and alkalinity, and (4) decreased electron donors and pH.

A two-stage system for octachlorinated dibenzo-p-dioxin (OCDD)-contaminated soil remediation was developed. Soil washing using emulsified oil (EO) was applied in the first stage for OCDD extraction followed by the second stage of bioremediation using P. mendocina NSYSU for remaining OCDD biodegradation. The major tasks included (1) determination of optimal soil washing conditions for OCDD extraction by EO, (2) evaluation of feasibility of OCDD biodegradation by P. mendocina NSYSU under aerobic cometabolic conditions using EO as the primary substrate, and (3) assessment of the effectiveness of OCDD removal using the two-stage system. During the soil washing stage, EO with two different oil-to-water ratios (1:50 and 1:200) and pore volumes were tested with initial soil OCDD concentration of 21,000 µg/kg. Results indicate that EO could effectively improve the solubility and desorption of OCDD in soils. Up to 74% of OCDD removal could be obtained after washing with 60 PVs of EO and dilution factor of 50. After the soil washing process, enriched P. mendocina NSYSU solution was added into the reactor to enhance the aerobic biodegradation of remaining OCDD in soils. P. mendocina NSYSU could use adsorbed EO globules as substrates and caused significant OCDD degradation via the aerobic cometabolic mechanism. Approximately 82% of the remaining OCDD could be removed after 50 days of operation, and P. mendocina NSYSU played important roles in OCDD biodegradation. Up to 87% of OCDD was removed through the EO washing and biodegradation process. The two-stage system is a potential technology to remediate dioxin-contaminated soils.

Tetrachloroethene (PCE) is one of the commonly found toxic chemicals in chlorinated-solvent contaminated groundwater. The objective of this study was to evaluate the effectiveness of enhanced bioremediation of PCE-contaminated groundwater with the supplement of nanoscale zero-valent iron (nZVI) contained long-lasting emulsified colloidal substrate (LECS) at a PCE-contaminated site. Approximately 50 L of LECS solution was pressured-injected into a remediation well (RW) and groundwater samples were collected from RW, two downgradient monitor wells (MWs), a control well, and a background well periodically for analyses. Injected LECS caused a rapid increase of the total organic carbon (TOC) concentration (up to 874 mg/L), and the increased TOC resulted in the transformation of the oxidation-reduction potential from aerobic to anaerobic stage. The pH was maintained in neutral due to the release of hydroxide ion after the oxidation of nZVI. Biodegradative reductive dechlorination of PCE was significantly enhanced, and PCE dropped from 0.5 to 0.01 mg/L after 130 days of operation. PCE dechlorination byproducts were also observed in downgradient MWs. Real-time polymerase chain reaction (PCR) results show that the populations of Dehalococcoides spp. (DHC) and Desulfitobacterium spp. (DSB) increased from 2 × 10 3 to 1.2 × 10 7 and from 1 × 10 3 to 7.4 × 10 6 cell/L after 60 days of operation in RW, respectively. DHC population was mainly composed of DHC in Victoria and Pinellas subgroups. Results demonstrate that supplement of LECS in PCE-polluted groundwater can effectively contain the PCE plume and accelerate the PCE dechlorinating rate under anaerobic conditions.

To enhance the reductive dechlorination of 1,2-dichloroethane (DCA) in groundwater, substrate injection may be required. However, substrate biodegradation causes groundwater acidification and sulfide production, which inhibits the bacteria responsible for DCA dechlorination and results in an odor problem. In the microcosm study, the effectiveness of the addition of ferrous sulfate (FS), desulfurization slag (DS), and nanoscale zero-valent iron (nZVI) on acidification and sulfide control was studied during reductive dechlorination of DCA, and the emulsified substrate (ES) was used as the substrate. Up to 94% of the sulfide was removed with FS and DS addition (0.25 wt%) (initial DCA concentration = 13.5 mg/L). FS and DS amendments resulted in the formation of a metal sulfide, which reduced the hydrogen sulfide concentration as well as the subsequent odor problem. Approximately 96% of the DCA was degraded under reductive dechlorination with nZVI or DS addition using ES as the substrate. In microcosms with nZVI or DS addition, the sulfide concentration was reduced to less than 15 μg/L. Acidification can be controlled via hydroxide ions production after nZVI oxidation and reaction of free CaO (released from DS) with water, which enhanced DCA dechlorination. The quantitative polymerase chain reaction results confirmed that the microcosms with nZVI added had the highest Dehalococcoides population (up to 2.5 × 10(8) gene copies/g soil) due to effective acidification control. The α-elimination mechanism was the main abiotic process, and reductive dechlorination dominated by Dehalococcides was the biotic mechanism that resulted in DCA removal. More than 22 bacterial species were detected, and dechlorinating bacteria existed in soils under alkaline and acidic conditions.

The objectives of this pilot-scale study were to evaluate the effectiveness of trichloroethylene (TCE)-contaminated groundwater in situ remediation by intrinsic and enhanced bioremediation mechanisms. Under enhanced bioremediation processes, leaked fuel oil served as the primary substrate to activate the reductive dechlorination of TCE. Real-time polymerase chain reaction (PCR) was performed to evaluate the variation in TCE-dechlorinating bacteria (Dehalococcoides spp.). Within the fuel oil plume, the natural attenuation of total petroleum hydrocarbon (TPH) and enhanced bioremediation of TCE was observed, and the evidence was as follows: (1) decreased TPH and TCE concentrations along the groundwater flow path; (2) depletion of the electron acceptor [dissolved oxygen (DO), nitrate, and sulfate)] concentrations; (3) production of dissolved ferrous iron, sulfide, methane, and CO2; (4) production of TCE degradation byproducts; (5) increased alkalinity and microbial populations; (6) decreased pH and oxidation–reduction potential and (7) increased Dehalococcoides. The results from PCR, denaturing gradient gel electrophoresis, and nucleotide sequence analysis revealed that 32 bacterial species that might be related to TPH and TCE degradation had been verified within the TPH plume. The results indicate that natural attenuation mechanisms could effectively contain the TPH plume and cause TPH removal. Although dechlorination of TCE was observed after the fuel oil spill, the dechlorination rate was not sufficient for TCE plume control due to the limited biodegradable primary substrates released from the fuel oil. An alternative primary substrate supplement was necessary to increase the substrate concentrations to obtain a higher TCE dechlorination rate. The results indicate that Dehalococcoides spp., which was one of the dominant bacteria in the groundwater at the site, can be used as a biomarker to evaluate the efficacy of TCE dechlorination.

The effectiveness of using gamma poly-glutamic acid (γ-PGA) as the primary carbon and nitrogen sources to bioremediate trichloroethene (TCE)-contaminated groundwater was studied in this pilot-scale study. γ-PGA (40 L) solution was injected into the aquifer via the injection well (IW) for substrate supplement. Groundwater samples were collected from monitor wells and IW and analyzed for TCE and its byproducts, geochemical indicators, dechlorinating bacteria, and microbial diversity periodically. Injected γ-PGA resulted in an increase in total organic carbon (TOC) (up to 9820 mg/L in IW), and the TOC biodegradation caused the formation of anaerobic conditions. Increased ammonia concentration (because of amine release from γ-PGA) resulted in the neutral condition in groundwater, which benefited the growth of Dehalococcoides. The negative zeta potential and micro-scale diameter of γ-PGA allowed its globule to distribute evenly within soil pores. Up to 93% of TCE removal was observed (TCE dropped from 0.14 to 0.01 mg/L) after 59 days of γ-PGA injection, and TCE dechlorination byproducts were also biodegraded subsequently. Next generation sequence (NGS) analyses were applied to determine the dominant bacterial communities. γ-PGA supplement developed reductive dechlorinating conditions and caused variations in microbial diversity and dominant bacterial species. The dominant four groups of bacterial communities including dechlorinating bacteria, vinyl chloride degrading bacteria, hydrogen producing bacteria, and carbon biodegrading bacteria.

Summary The Ju-Liao Stream is one of the most contaminated streams in Kaohsiung City, Taiwan. A constructed wetland (CW) system was built in 2010 for polluted stream water purification and ecosystem improvement. An aerated gravel-packed contact bed (CB) system was built in 2011 and part of the stream water was treated by the CB before discharging to the CW. The influent rates of the CW and CB were approximately 5570 and 900 m3/d, respectively. The CW contained one free-water surface basin planted with emergent wetland plants, followed by the plug-flow channel-shaped free-water surface basin planted with emergent and floating wetland plants. The mean measured hydraulic loading rate (HLR), hydraulic retention time (HRT), water depth, and total volume of wetland system were 1.7 m/d, 0.68 d, 0.7 m, and 4400 m3, respectively. The aeration zone of the CB system had a dimension of 24 m (L) × 8 m (W) × 3 m (H), which was filled with gravels (average diameter = 5 cm) with a porosity of 0.4, and the aeration rate was 7.8 m3/min. Results show that the CB system was able to remove 69% of suspended solid (SS), 86% of biochemical oxygen demand (BOD), and 58% of total nitrogen (TN). Up to 82% of BOD and 27% of TN could be removed in the CW system. Removal efficiency of SS was affected by the growth of chlorophyll a in the CW system due to the growth of algae. The observed first-order decay rates (k) for BOD and TN in CB were 9.3 and 4.2 1/d, and the k values for BOD and TN removal in CW were 2.5 and 0.45 1/d. The high pollutant removal efficiencies in the CB system indicate that the system could enhance the organic and nutrient removal through the biological processes effectively. Sediments contained high total organic matter (1.9–4.5%), sediment total nitrogen (6.4–10.1 g/kg), sediment total phosphorus (0.59–0.94 g/kg), and sediment oxygen demand (0.9–4.1 g O2/m2 d). The organic and nutrient-abundant sediments resulted in reduced conditions (oxidation–reduction potential measurements

A long-lasting emulsified colloidal substrate (LECS) was developed for continuous carbon and nanoscale zero-valent iron (nZVI) release to remediate trichloroethylene (TCE)-contaminated groundwater under reductive dechlorinating conditions. The developed LECS contained nZVI, vegetable oil, surfactants (Simple Green™ and lecithin), molasses, lactate, and minerals. An emulsification study was performed to evaluate the globule droplet size and stability of LECS. The results show that a stable oil-in-water emulsion with uniformly small droplets (0.7 μm) was produced, which could continuously release the primary substrates. The emulsified solution could serve as the dispensing agent, and nZVI particles (with diameter 100–200 nm) were distributed in the emulsion evenly without aggregation. Microcosm results showed that the LECS caused a rapid increase in the total organic carbon concentration (up to 488 mg/L), and reductive dechlorination of TCE was significantly enhanced. Up to 99% of TCE (with initial concentration of 7.4 mg/L) was removed after 130 days of operation. Acidification was prevented by the production of hydroxide ion by the oxidation of nZVI. The formation of iron sulfide reduced the odor from produced hydrogen sulfide. Microbial analyses reveal that dechlorinating bacteria existed in soils, which might contribute to TCE dechlorination.

The objectives of this pilot-scale study were to (1) evaluate the effectiveness of bioremediation of trichloroethylene (TCE)-contaminated groundwater with the supplement of slow polycolloid-releasing substrate (SPRS) (contained vegetable oil, cane molasses, surfactants) under reductive dechlorinating conditions, (2) apply gene analyses to confirm the existence of TCE-dechlorinating genes, and (3) apply the real-time polymerase chain reaction (PCR) to evaluate the variations in TCE-dechlorinating bacteria (Dehalococcoides spp.). Approximately 350 L of SPRS solution was supplied into an injection well (IW) and groundwater samples were collected and analyzed from IW and monitor wells periodically. Results show that the SPRS caused a rapid increase of the total organic carbon concentration (up to 5794 mg/L), and reductive dechlorination of TCE was significantly enhanced. TCE dechlorination byproducts were observed and up to 99% of TCE removal (initial TCE concentration = 1872 μg/L) was observed after 50 days of operation. The population of Dehalococcoides spp. increased from 4.6 × 101 to 3.41 × 107 cells/L after 20 days of operation. DNA sequencing results show that there were 31 bacterial species verified, which might be related to TCE biodegradation. Results demonstrate that the microbial analysis and real-time PCR are useful tools to evaluate the effectiveness of TCE reductive dechlorination.

Cinnapine (1), a new pyridine alkaloid, has been isolated from the roots of Cinnamomum philippinense (Lauraceae). The structure was characterized and identified by spectral analysis.

Reverse osmosis (RO) membrane technology cannot be applied for the direct purification of nano-scale particle-containing wastewater due to membrane fouling/clogging problems. In this study, a three-stage pre-treatment process was developed to remove nano-scale particles from wastewater prior to further water purification using RO. The proposed pre-treatment system involved chemical coagulation/flocculation followed by sand filtration (SF) and ultrafiltration (UF). Backgrinding (BG) wastewater, which contained high concentrations of inorganic nano-particles, was used to evaluate the feasibility of this three-stage process for nano-scale particle removal. The BG wastewater (collected from a semiconductor manufacturing plant) had turbidity and suspended solids concentrations of approximately 1403 NTU and 77.8 mg/L, respectively. Up to 98% of the turbidity and particles could be removed through chemical coagulation/flocculation when 2.2 mg/L of polyaluminum chloride was used as coagulant and 0.5 mg/L of polymer was used as flocculant. However, the SF system could not effectively remove the nano-scale particles from BG wastewater directly. More than 99% of the turbidity and particles could be removed with the application of a UF unit [spiral-wound (SW) or hollow-fiber (HF) membrane] after the coagulation/flocculation/SF processes. Results indicate that the three-stage system is appropriate for the pre-treatment of nano-particle-containing wastewater.

The objective of this study was to evaluate the effectiveness of in situ bioremediation of trichloroethylene (TCE)-contaminated groundwater using specific gene analyses under the following conditions: (1) pretreatment with biodegradable surfactants [Simple Green™ (SG) and soya lecithin (SL)] to enhance TCE desorption and dissolution, and (2) supplementation with SG, SL, and cane molasses as primary substrates to enhance the aerobic cometabolism of TCE. Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and nucleotide sequence analysis were applied to monitor the variations in specific activity-dependent enzymes and dominant microorganisms. Results show that TCE-degrading enzymes, including toluene monooxygenase, toluene dioxygenase, and phenol monooxygenase, were identified from sediment samples collected from a TCE-spill site. Results from the microcosm study show that addition of SG, SL, or cane molasses can enhance the aerobic cometabolism of TCE. The TCE degradation rates were highest in microcosms with added SL, the second highest in microcosms containing SG, and lowest in microcosms containing cane molasses. This indicates that SG and SL can serve as TCE dissolution agents and act as primary substrates for indigenous microorganisms. Four dominant microorganisms (Rhodobacter sp., Methyloversatilis sp., Beta proteobacterium sp., and Hydrogenophaga pseudoflava) observed in microcosms might be able to produce TCE-degrading enzymes for TCE cometabolic processes.

Wastewater discharged from semiconductor industry contains high turbidity and conductivity. When membrane systems are used for semiconductor wastewater reclamation without pretreatment, membranes will soon be clogged by the nano-size particles. In this pilot-scale study, a three-stage system has been developed for semiconductor wastewater reclamation. This system (flow = 5 m 3 d − 1 ) contained fiber ball (material: polypropylene, diameter = 25 mm) filtration (FF) followed by ultrafiltration (UF) and reverse osmoses (RO) units. The FF was installed as a pretreatment unit (first stage) for particle removal. Spiral wound (SW) UF (material: polyethersulfone) and RO membrane (material: polyamide) were applied in the second and third treatment stages, respectively. Up to 95.8% of suspended solid (SS) could be removed after the pretreatment by FF system with an 88 m hr − 1 filtration velocity. More than 95% of the remaining turbidity can be further removed after the following UF treatment (turbidity dropped from 30 to below 0.6 NTU). After the treatment by RO unit, the effluent conductivity, turbidity, and total dissolved solid dropped to below 69.2 μS cm − 1 , 0.06 NTU, and 53.5 mg L − 1 , respectively. Knowledge obtained from this study will be useful in designing the three-stage FF and UF/RO system for practical application.

The Salt-water River watershed is one of the major river watersheds in the Kaohsiung City, Taiwan. Water quality and sediment investigation results show that the river water contained high concentrations of organics and ammonia-nitrogen, and sediments contained high concentrations of heavy metals and organic contaminants. The main pollution sources were municipal and industrial wastewaters. Results from the enrichment factor (EF) and geo-accumulation index (Igeo) analyses imply that the sediments can be characterized as heavily polluted in regard to Cd, Cr, Pb, Zn, and Cu. The water quality analysis simulation program (WASP) model was applied for water quality evaluation and carrying capacity calculation. Modeling results show that the daily pollutant inputs were much higher than the calculated carrying capacity (1050 kg day(-1) for biochemical oxygen demand and 420 kg day(-1) for ammonia-nitrogen). The proposed watershed management strategies included river water dilution, intercepting sewer system construction and sediment dredging.

The Houjing River watershed is one of the three major river watersheds in the Kaohsiung City, Taiwan. Based on the recent water quality analysis, the Houjing River is heavily polluted. Both point and non-point source (NPS) pollutants are the major causes of the poor water quality in the Houjing River. Investigation results demonstrate that the main point pollution sources included municipal, agricultural, and industrial wastewaters. In this study, land use identification in the Houjing River watershed was performed by integrating the skills of geographic information system (GIS) and global positioning system (GPS). Results show that the major land-use patterns in the upper catchment of the Houjing River watershed were farmlands, and land-use patterns in the mid to lower catchment were residential and industrial areas. An integrated watershed management model (IWMM) and Enhanced Stream Water Quality Model (QUAL2K) were applied for the hydrology and water quality modeling, watershed management, and carrying capacity calculation. Modeling results show that the calculated NH3-N carrying capacity of the Houjing River was only 31 kg/day. Thus, more than 10,518 kg/day of NH3-N needs to be reduced to meet the proposed water quality standard (0.3 mg/L). To improve the river water quality, the following remedial strategies have been developed to minimize the impacts of NPS and point source pollution on the river water quality: (1) application of BMPs [e.g. source (fertilizer) reduction, construction of grassy buffer zone, and land use management] for NPS pollution control; (2) application of river management scenarios (e.g. construction of the intercepting and sewer systems) for point source pollution control; (3) institutional control (enforcement of the industrial wastewater discharge standards), and (4) application of on-site wastewater treatment systems for the polishment of treated wastewater for water reuse.

This study investigated the feasibility of applying ozone (O 3 ) to reduce the color content of wastewater caused by two commercial reactive dyes (Blue-19 and Orange-13). In the bench-scale experiment, experimental parameters including pH, ozone dosage, and reaction time were evaluated in a 14-L reactor to obtain the optimal operating conditions. Results show that ozone dosage and pH dominated the effectiveness of the decolorization process. The color content could be reduced from 2000 to 200 ADMI (American Dye Manufacture Institute) values within a reaction time of 30 min with the ozone input rate of 2.66 g/h. The pH values of 3 and 10 favored decolorization of Blue-19 and Orange-13, respectively. This was due to the effects that reactive and oxidizing species of molecular ozone and hydroxyl radicals were predominant at low and high pH, respectively. Moreover, molecular ozone was more selective to certain dye structures during the oxidation process. Kinetic analyses show that decolorization of Orange-13 and Blue-19 followed first-order kinetics. The degree of decolorization was primarily proportional to the ozone dosage. Results from this study provide insights into the characteristics and mechanisms of decolorization by the O 3 technique. Results will also aid in designing a system for practical application.

In this study, microcosm experiments were conducted to evaluate the effectiveness of (1) nutrients, hydrogen peroxide ( H2 O2 ) , and cane molasses addition; (2) soil washing by biodegradable surfactant [Simple Green (SG)]; and (3) soil pretreatment by Fenton-like oxidation on the bioremediation of fuel-oil contaminated soils. The dominant native microorganisms in the fuel-oil contaminated soils after each treatment process were determined via polymerase chain reaction, denaturing gradient gel electrophoresis, and nucleotide sequence analysis. Results show that approximately 32 and 56% of total petroleum hydrocarbon (TPH) removal (initial concentration of 5,000 mg kg−1 ) were observed in microcosms with the addition of nutrient and cane molasses ( 1,000 mg L−1 ) , respectively, compared to only 9% of TPH removal in live control microcosms under intrinsic conditions (without amendment) after 120 days of incubation. Addition of cane molasses would cause the increase in microbial population and thus enhanc...

The industrial solvent trichloroethylene (TCE) is among the most ubiquitous chlorinated solvents found in groundwater contamination. The main objectives of this study were to evaluate the feasibility of using non-ionic surfactant Simple Green™ (SG) to enhance the oxidative dechlorination of TCE by potassium permanganate (KMnO 4 ) employing a continuous stir batch reactor system (CSBR) and column experiments. The effect of using surfactant SG to enhance the biodegradation of TCE via aerobic cometabolism was also examined. Results from CSBR experiments revealed that combination of KMnO 4 with surfactant SG significantly enhanced contaminant removal, particularly when the surfactant SG concentrated at its CMC. TCE degradation rates ranged from 74.1% to 85.7% without addition of surfactant SG while TCE degradation rates increased to ranging from 83.8% to 96.3% with presence of 0.1 wt% SG. Furthermore, results from column experiments showed that TCE was degraded from 38.1 μM to 6.2 μM in equivalent to 83.7% of TCE oxidation during first 560 min reaction. This study has also demonstrated that the addition of surfactant SG is a feasible method to enhance bioremediation efficiency for TCE contaminated groundwater. The complete TCE degradation was detected after 75 days of incubation with both 0.01 and 0.1 wt% of surfactant SG addition. Results revealed that surfactant enhanced chemical oxidation and bioremediation technology is one of feasible approaches to clean up TCE contaminated groundwater.

In-circuit emulators have become part of the permanent structure of microprocessor cores to support on-chip test and debug activities in highly integrated environments such as SoCs. However, ICE design styles and operation principles are quite diverse. This article presents a taxonomy based on the notions of foreground and background operations and hardwaresoftware implementation alternatives to organize existing in-circuit emulation approaches.

Kaolins contaminated with heavy metals, Cu and Pb, and organic compounds, p -xylene and phenanthrene, were treated with an upward electrokinetic soil remediation (UESR) process. The effects of current density, cathode chamber flushing fluid, treatment duration, reactor size, and the type of contaminants under the vertical non-uniform electric field of UESR on the simultaneous removal of the heavy metals and organic contaminants were studied. The removal efficiencies of p -xylene and phenanthrene were higher in the experiments with cells of smaller diameter or larger height, and with distilled water flow in the cathode chamber. The removal efficiency of Cu and Pb were higher in the experiments with smaller diameter or shorter height cells and 0.01 M HNO 3 solution as cathode chamber flow. In spite of different conditions for removal of heavy metals and organics, it is possible to use the upward electrokinetic soil remediation process for their simultaneous removal. Thus, in the experiments with duration of 6 days removal efficiencies of phenanthrene, p -xylene, Cu and Pb were 67%, 93%, 62% and 35%, respectively. The experiment demonstrated the feasibility of simultaneous removal of organic contaminants and heavy metals from kaolin using the upward electrokinetic soil remediation process.

An upward electrokinetic soil remedial (UESR) technology was proposed to remove heavy metals from contaminated kaolin. Unlike conventional electrokinetic treatment that uses boreholes or trenches for horizontal migration of heavy metals, the UESR technology, applying vertical non-uniform electric fields, caused upward transportation of heavy metals to the top surface of the treated soil. The effects of current density, treatment duration, cell diameter, and different cathode chamber influent (distilled water or 0.01 M nitric acid) were studied. The removal efficiencies of heavy metals positively correlated to current density and treatment duration. Higher heavy metals removal efficiency was observed for the reactor cell with smaller diameter. A substantial amount of heavy metals was accumulated in the nearest to cathode 2 cm layer of kaolin when distilled water was continuously supplied to the cathode chamber. Heavy metals accumulated in this layer of kaolin can be easily excavated and disposed off. The main part of the removed heavy metals was dissolved in cathode chamber influent and moved away with cathode chamber effluent when 0.01 M nitric acid was used, instead of distilled water. Energy saving treatment by UESR technology with highest metal removal efficiencies was provided by two regimes: (1) by application of 0.01 M nitric acid as cathode chamber influent, cell diameter of 100 mm, duration of 18 days, and constant voltage of 3.5 V (19.7 kWh/m3 of kaolin) and (2) by application of 0.01 M nitric acid as cathode chamber influent, cell diameter of 100 cm, duration of 6 days, and constant current density of 0.191 mA/cm2 (19.1 kWh/m3 of kaolin).

One new chromone, 5,7-dihydroxy-8-methoxychromone (1), was isolated from the peels of Citrus reticulata (Rutaceae), together with two polymethoxyflavones, 5,6,7,8,3′,4′-hexamethoxyflavone (2) and 5,6,7,8,4′-pentamethoxyflavone (3). The structure of 1 was characterized and identified by spectral analysis.

Our objective is to develop a feasible, low-cost, retargetable embedded ICE-with as few modifications to the standard as possible-that can be easily integrated with microprocessor cores at the register-transfer level. This module, which can be embedded with a microprocessor core, makes real-time, online debugging at full speed possible with a minor gate-count overhead.