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Large numbers of mice were exposed to 2‐acetylaminofluorene (2‐AAF) in the diet in order to provide quantitative information on carcinogenic dose‐response relationships. Weanling BALB/c and C57BL/6 mice of both sexes were fed 2‐AAF ad libitum in their diet for 18 months at concentrations of 0, 100, 250, and 500 ppm. Occurrence of a bladder tumor was considered as the endpoint for determination of dose‐response curves. Data were accumulated from mice that died during the 18‐month period, from moribund mice that were necropsied, and from mice necropsied at the terminal sacrifice after 18 months of exposure to the carcinogen. A dose response was noted for the body weights of all animals and for the mortality rate and bladder tumor development of the females. In the males the dose of 500 ppm of 2‐A A F appeared to be above the maximum tolerated dose and resulted in early death of the animals, a decreased tumor incidence, and the lack of a dose‐related response. Bladder tumor development in the females followe...