26 results on '"Debruyne, Lies"'
Search Results
2. Participation and co-theorising: How stakeholder interests and scientific outputs clash in the Horizon 2020 multi-actor approach.
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van der Velden, Daniel, Debruyne, Lies, Klerkx, Laurens, and Dessein, Joost
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The multi-actor approach in the EU's Horizon 2020 program has seen use across a large number of research projects. However, there remain questions about the extent and depth of participation that is achieved in these research projects, and how it may enable joint production of scientific theory next to readily applicable practical knowledge. This article aims to explore the latter question by focusing on the potential of such co-theorising, understood as the involvement of stakeholders in producing scientific outputs. We analyse how researchers involved in this research project view the participation of stakeholders, how they deal with tensions in participation, and how these tensions are resolved. Through 17 semi-structured interviews with researchers involved in the project's Living Labs we show that there are tensions between the interests of stakeholders, the project requirements and research interests. The findings indicate that a focus on including the stakeholders in theorising comes at a cost of practical relevance to the stakeholder. To safeguard the practical relevance of participation, researchers choose to exclude discussion on theory and theoretical concepts from the living labs. This is despite an initial belief that co-theorising and the further participation of stakeholders in producing scientific outputs holds merit. Hence, researchers purposefully prioritize practical relevance to stakeholders, thereby reducing the potential for co-theorising. The fact that theories already need to be defined in a proposal stage and project's limit scope for redefinition of core concepts, make that advancing theory together with stakeholders is not well accommodated for in Horizon 2020. This also bears relevance for the Horizon Europe program which follows a similar approach. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Power to the Facilitated Agricultural Dialogue: An Analysis of On-Farm Demonstrations as Transformative Learning Spaces
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Cooreman, Hanne, Debruyne, Lies, Vandenabeele, Joke, and Marchand, Fleur
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Purpose: It remains a critical question how to support farmers to develop towards more sustainable practices. Earlier experiences reveal that on-farm demonstrations (OFDs) can be part of the answer. Therefore, we investigate OFDs as potential transformative learning spaces. Methodology: We apply a mixed methods approach, using an observation tool, participant surveys and telephone interviews. We compare 15 different OFDs, divided into two groups: OFDs with and without facilitated dialogue. We investigated differences based on core inducing processes of transformative learning: disorienting dilemma and (self-)reflection. Additionally we investigated the adoption decision making process half a year after the OFD took place. Findings: Participants in facilitated dialogue OFDs agreed significantly more on experiencing surprise, an indication of disorienting dilemma, and on having reflected and learned. Most mentioned adoption barriers are a lack of relevance for the specific situation and a need for more information. Most mentioned suggestions indicate a request for more real life application. Practical implications: This research indicates that OFDs with facilitated dialogue can trigger more cognitive conflict and reflection processes of attendees, and thus support learning processes on (more) sustainable agricultural practices, as opposed to OFDs without deliberate dialogue facilitation. Theoretical implications: Our study demonstrates that transformative learning theory can inform research on agricultural learning spaces, such as OFDs, on effective triggers to support learning and competencies towards more sustainable agriculture. Originality: Based on rich empirical quantitative and qualitative data we reveal that facilitated dialogue enhances learning during OFDs, but this does not seem to be a common practice.
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- 2021
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4. Situating Demonstrations within Contemporary Agricultural Advisory Contexts: Analysis of Demonstration Programmes in Europe
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Ingram, Julie, Chiswell, Hannah, Mills, Jane, Debruyne, Lies, Cooreman, Hanne, Koutsouris, Alex, Alexopoulos, Yiorgos, Pappa, Eleni, and Marchand, Fleur
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Purpose: The paper aims to examine interactions between demonstrations at programme level and Agricultural Advisory Services (AAS). It situates analysis of the demonstration programme activities within contemporary advisory contexts, asking: how do demonstration programmes interact with the AAS in which they are situated; and what role do demonstration programmes play in enhancing and contributing to the AAS? Methodological approach: Data, pertaining to organisational arrangements, were collected using interviews and workshops representing 35 demonstration programmes across Europe. Themes from the literature together with emergent themes were used to progressively unpack and understand the interaction between the programmes and the AAS. Findings: Demonstration organisational arrangements show different degrees of embedding in, and adapting to, the AAS. Embedding is being incorporated into existing formalised structures and is more likely in the AAS with a low level of pluralism. Adaptation occurs through collaboration, partnership and networking and is more likely in more pluralistic AAS. Practical Implications: The need to support demonstration programmes to create more stable networks, to strengthen their linking role in the AAS, and to foster strategies for the progression of farmers' learning was identified. Theoretical implications: The paper contributes to the literature by providing insights at the demonstration programme level (as opposed to farm or event level) and revealing embedded and adaptive processes with many interdependencies between the programmes and AAS components. Originality: This paper opens up new perspectives on understanding how demonstrations are positioned in contemporary AAS contexts, looking at AAS influences on delivering demonstrations beyond farm and event level methods.
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- 2021
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5. Effectiveness of On-Farm Demonstration Events in the EU: Role of Structural Characteristics
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Marchand, Fleur, Cooreman, Hanne, Pappa, Eleni, Perifanos, Ioanis, Alexopoulos, Yiorgos, Debruyne, Lies, Chiswell, Hannah, Ingram, Julie, and Koutsouris, Alex
- Abstract
Purpose: The objective of this paper is to undertake an in-depth exploration of how structural characteristics of on-farm demonstration events impact the effectiveness of the demonstration as perceived by participants. Methodology: An explanatory mixed-methods approach was used based on the responses to 356 post-demonstration questionnaires filled out by the attendees of 31 demonstration events held in 12 EU countries in 2018, together with the qualitative data from an observation tool, interviews and case study reports relating to the same 31 events. Factor analysis was employed to predict general effectiveness, complemented with a qualitative analysis of participants' responses to improve the understanding of the quantitative results. Findings: Results indicate that peer-to-peer learning activities and good facilitation increase participants' perception of the effectiveness of a demonstration event. Furthermore, characteristics such as the structure of the day, the suitability of the host farm, a trustworthy demonstrator, and group size add much to the effectiveness of a demo event. Group dynamics -- including group connectedness and composition -- have the potential to impact perceived effectiveness. Practical implications: The paper contains clear messages for practitioners when designing a demonstration event. Theoretical implications: Our results connect with theories on motivation and adult learning in the context of farmer learning during on-farm demonstration. Originality: The paper is based on a rich empirical dataset. It reveals that group size and learning methods are crucial to achieve effective learning outcomes during demonstration events.
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- 2021
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6. Inclusivity of On-Farm Demonstration: Gender, Age, and Geographic Location
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Sutherland, Lee-Ann, Burton, Rob J. F., Adamsone-Fiskovica, Anda, Hardy, Claire, Elzen, Boelie, Debruyne, Lies, and Flanigan, Sharon
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Purpose: To assess the inclusivity of on-farm demonstration across Europe, in relation to age, gender, and geographical location of participants. Methodology: The paper is based on a survey of 1162 on-farm demonstrators (farmers and organisations) and three supra-regional workshops. Findings: Overall, on farm-demonstrations were found to be engaging young(er) farmers who are at a career stage of being able to implement long-term innovations. However, across Europe demonstrations were primarily attended by men. On-farm demonstrations were most common in Northern Europe, where advisory services brought together multiple AKIS actors. There were fewer on-farm demonstrations in Southern Europe, where demonstrations were more likely to be led by research institutes or individual farmers. Eastern Europe is notable for greater diversity in terms of gender and age of demonstration participants. Within countries, on-farm demonstrations occurred more frequently in regions of high agricultural profitability; more remotely located farmers had fewer opportunities to participate. Practical implications: Demonstrations led by public and privately funded advisory services appear to attract primarily male farmers, thus reinforcing gendered patterns of participation in European agriculture. The location of advisory services and research institutes in high profitability locales disproportionately privileges farmers located there. More targeted efforts are required to ensure the participation of farmers who are female, older and located in less productive regions. Theoretical implications: The paper draws attention to the lack of inclusivity of on-farm demonstration, developing a conceptual framework based on Lukes' three faces of power. Originality: The paper utilises the first European-wide inventory of on-farm demonstration to assess inclusivity.
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- 2021
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7. Development and validation of an instrument to measure the vision of European agricultural advisors towards innovation.
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Lybaert, Charlotte, Debruyne, Lies, Kyndt, Eva, and Marchand, Fleur
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EXPLORATORY factor analysis ,CONFIRMATORY factor analysis ,AGRICULTURAL development ,LINEAR systems ,CONSORTIA - Abstract
Purpose: This article describes the development and validation of a survey designed to measure the vision of European agricultural advisors towards innovation. Design/Methodology/Approach: The items of the instrument were developed based on the conceptual framework provided by the position paper for the Transformative Innovation Policy Consortium. The resulting instrument was completed by 656 advisors recruited through the network of the European Horizon 2020 i2connect project. The data was divided into two random subsets. The structure of the instrument was explored using an exploratory factor analysis (EFA) using one subset and convergent validity was tested by applying confirmatory factor analysis (CFA) to the second subset. Findings: The EFA resulted in a three-factor solution. In accordance with the conceptual framework, these factors were labelled (a) linear innovation, (b) innovation systems and (c) transformative change. The CFA demonstrated an adequate fit and a satisfactory level of internal consistency. Practical implications: The instrument can assist in eliciting advisors' views on innovation, which could be used in advisors' selection and recruitment. Theoretical implications: Building on a strong conceptual basis, the paper presents a theoretically robust instrument for assessing advisors' views on different innovation models. Such assessments could in turn lead to further expand and elaborate concepts on this aspect. Originality/Value: The survey is the first instrument to include the emerging policy paradigm of transformative change and as such allows measuring the degree to which European advisors are willing to support transformative innovation policy. [ABSTRACT FROM AUTHOR]
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- 2024
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8. How do current sustainability assessment tools support farmers’ strategic decision making?
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Coteur, Ine, Wustenberghs, Hilde, Debruyne, Lies, Lauwers, Ludwig, and Marchand, Fleur
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- 2020
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9. Structuring the myriad of sustainability assessments in agri-food systems: A case in Flanders
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Coteur, Ine, Marchand, Fleur, Debruyne, Lies, and Lauwers, Ludwig
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- 2019
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10. Farmers' reasons to accept bio-based fertilizers: A choice experiment in seven different European countries
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Tur-Cardona, Juan, Bonnichsen, Ole, Speelman, Stijn, Verspecht, Ann, Carpentier, Louise, Debruyne, Lies, Marchand, Fleur, Jacobsen, Brian H., and Buysse, Jeroen
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- 2018
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11. Participatory tuning agricultural sustainability assessment tools to Flemish farmer and sector needs
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Coteur, Ine, Marchand, Fleur, Debruyne, Lies, Dalemans, Floris, and Lauwers, Ludwig
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- 2018
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12. A new agri-food systems sustainability approach to identify shared transformation pathways towards sustainability
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Hubeau, Marianne, Marchand, Fleur, Coteur, Ine, Mondelaers, Koen, Debruyne, Lies, and Van Huylenbroeck, Guido
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- 2017
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13. A reflexive assessment of a regional initiative in the agri-food system to test whether and how it meets the premises of transdisciplinary research
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Hubeau, Marianne, Marchand, Fleur, Coteur, Ine, Debruyne, Lies, and Van Huylenbroeck, Guido
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- 2018
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14. A framework for guiding sustainability assessment and on-farm strategic decision making
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Coteur, Ine, Marchand, Fleur, Debruyne, Lies, Dalemans, Floris, and Lauwers, Ludwig
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- 2016
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15. Cyborg farmers: Embodied understandings of precision agriculture.
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Velden, Daniel van der, Klerkx, Laurens, Dessein, Joost, and Debruyne, Lies
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PRECISION farming ,CYBORGS ,FARMERS ,FARMERS' attitudes ,AGRICULTURE ,AGRICULTURAL technology - Abstract
Precision agriculture is often seen as disembodied and placeless, promised to either bring about a fourth agricultural revolution or as the start of dystopian rural futures, where farmers and their knowledge will be replaced by machines. A growing body of literature shows more nuanced ways of working with precision agriculture. This illustrates the need to investigate how, and under which conditions, precision agriculture is integrated and internalised by farmers. Based on 25 in‐depth interviews with male Dutch crop farmers, contractors, researchers, and ag‐tech developers, we develop the concept of the cyborg farmer, who embodies the use of precision technology while maintaining an intimate relationship with agro‐ecological context. This approach challenges the mind–body dichotomy in that the cognitive is not understood as primary, as emotions and the materiality of the body are taken seriously. This perspective emphasises the importance of embodied knowledge in how farmers interpret precision agriculture data. Our findings highlight the productive tension between data‐driven elements of precision agriculture and the embodied and intuitive understanding of agro‐ecological context. This productive tension consists of the successful integration of different forms of knowledge by the cyborg farmer, where embodied sense‐making and precision agriculture data are integrated into the formation of useable knowledge. The cyborg farmer maintains farmer agency and resists the dominance of algorithmic rationality over other forms of knowledge. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Reflection on the development process of a sustainability assessment tool : learning from a Flemish case
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Triste, Laure, Marchand, Fleur, Debruyne, Lies, Meul, Marijke, and Lauwers, Ludwig
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- 2014
17. Key characteristics for tool choice in indicator-based sustainability assessment at farm level
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Marchand, Fleur, Debruyne, Lies, Triste, Laure, Gerrard, Catherine, Padel, Susanne, and Lauwers, Ludwig
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- 2014
18. Constructing legitimacy for technologies developed in response to environmental regulation: the case of ammonia emission-reducing technology for the Flemish intensive livestock industry.
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van der Velden, Daniel, Dessein, Joost, Klerkx, Laurens, and Debruyne, Lies
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ANIMAL industry ,ENVIRONMENTAL regulations ,AGRICULTURAL intensification ,LIVESTOCK farms ,PERFORMANCE technology ,GREEN technology - Abstract
This study is focused on unsustainable agri-food systems, especially intensive livestock farming and its resulting environmental harms. Specifically we focus on the development of technologies that seek to mitigate these environmental harms. These technologies are generally developed as incremental innovations in response to government regulation. Critics of these technological solutions allege that these developments legitimate unsustainable food production systems and are incapable of supporting agri-food systems transformation. At the same time, technology developers and other actors seek to present these technologies as the legitimate solution to agri-environmental harms. Our study seeks to explore the perceptions and constructions of legitimacy for technologies that are developed to reduce ammonia emissions in intensive livestock farming in Flanders (Belgium). We use a qualitative case study, employing semi-structured interviews and workshops, with technology developers of ammonia-emission reducing technologies and stakeholders in the intensive livestock farming industry in Flanders. What our study shows is that technologies developed to reduce emissions are dependent on regulative legitimacy. The normative and cognitive legitimacy of these technologies is lacking, both due to ties to the intensive livestock industry and due to uncertainty over the performance of these technologies. With the delegitimation of intensive livestock farming, the legitimacy of these technologies is also under threat. In response, technology developers are looking to (re-)construct this legitimacy through knowledge claims over the performance of their technologies. We show several ways for other actors to deal with this, centred on either re-legitimising technologies to maintain the status quo, or to contest these knowledge claims and use them to disrupt path dependencies. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli
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Debruyne, Lies, Samyn, Emly, De Brandt, Evie, Vandenberg, Olivier, Heyndrickx, Marc, and Vandamme, Peter
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- 2008
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20. Digital transformation of agriculture and rural areas: A socio-cyber-physical system framework to support responsibilisation.
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Rijswijk, Kelly, Klerkx, Laurens, Bacco, Manlio, Bartolini, Fabio, Bulten, Ellen, Debruyne, Lies, Dessein, Joost, Scotti, Ivano, and Brunori, Gianluca
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RURAL geography ,SUSTAINABLE agriculture ,DAIRY farming ,AGRICULTURE ,DIGITAL technology ,RESPONSIBILITY - Abstract
Digital technologies are often seen as an opportunity to enable sustainable futures in agriculture and rural areas. However, this digital transformation process is not inherently good as it impacts on many aspects (e.g. economic, environmental, social, technological, institutional) and their relations. The responsible research and innovation approach calls for a better understanding and anticipation of the often unknown impacts. To meet this aim we have developed a framework that allows to gain insight on the relations between the social, the cyber and the physical, i.e. a socio-cyber-physical system and have described conditions for a successful digital transformation of such a system. These are design of, and creating access to digital technologies, and navigating system complexity. This framework allows for a better problematisation of digital transformation and has been illustrated through an example of digital dairy farming. It supports an enhanced understanding of moral responsibilities regarding digital transformation, fitting within the responsible research and innovation approach, as well as a better understanding who is responsible or accountable for the identified (positive or negative) impacts, i.e. responsibilisation. • Digital transformation requires Responsible Research and Innovation. • Responsible Research and Innovation requires clear problematisation. • A Socio-Cyber-Physical System for problematisation is presented. • This framework can support responsibilisation in digital transformation. • An illustration of the framework in digital dairy farming is given. [ABSTRACT FROM AUTHOR]
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- 2021
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21. Exploring participation in a sustainable farming initiative with self-determination theory.
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Triste, Laure, Vandenabeele, Joke, van Winsen, Frankwin, Debruyne, Lies, Lauwers, Ludwig, and Marchand, Fleur
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SUSTAINABLE agriculture ,SELF-determination theory ,EDUCATION of farmers ,MOTIVATION (Psychology) ,TRAINING of farmers - Abstract
Sustainable farming initiatives (SFIs), which aim to support farmers’ adoption of sustainable practices vary in the degree of farmer participation. In this study, we explored the influence of SFI design characteristics on farmer motivation to participate. Based on self-determination theory, we framed farmer participation by linking participants’ motives and motivational processes, participation context and participants’ perception of that context. Using a mixed-method investigation, we analysedVeldleeuwerik, a Dutch SFI with about 400 members. We observed four motive types, in order of importance: knowledge exchange, business opportunities, sustainability and social contact. By combining them with five types of motivational regulation, our research revealed that motives are directed by a diversity of underlying motivational processes, and that both are influenced by the participation context, or SFI design. Based on these results, we suggest that SFIs who wish to attract farmers who are not autonomously motivated for sustainability, need to rely on characteristics that meet either other motives than sustainability or other motivational processes than autonomous ones. To maintain participation, SFI characteristics should support autonomous motivational processes, by supporting the psychological basic needs of autonomy, competence and relatedness. The study illustrates how a socio-psychological perspective adds value to investigating SFI design characteristics for successful farmer participation. [ABSTRACT FROM AUTHOR]
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- 2018
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22. Reassessment of the taxonomy of Arcobacter cryaerophilus.
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Debruyne, Lies, Houf, Kurt, Douidah, Laid, De Smet, Sarah, and Vandamme, Peter
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TAXONOMY ,POLYMORPHISM (Zoology) ,HEAT shock proteins ,SPECIES diversity ,GENE expression ,DNA ,NUCLEIC acid hybridization - Abstract
Abstract: Arcobacter cryaerophilus is a heterogeneous species in which two distinct subgroups have been reported. In the present study, the taxonomic status of these subgroups was reassessed using amplified fragment length polymorphism and heat shock protein 60 gene sequence analysis. The results demonstrated that A. cryaerophilus has a complex taxonomic structure, which consists of multiple cores of strains that share intermediate levels of DNA–DNA hybridisation and exhibit low levels of DNA–DNA hybridisation towards other Arcobacter species. One of these cores consisted of the majority of strains and included most subgroup 2 strains from previous studies. A. cryaerophilus subgroup 1 strains represented three distinct cores, among which the type strain occupied a distinct position. These results therefore also demonstrate that the current subgroup nomenclature in A. cryaerophilus should be abandoned, and that the type strain is genetically aberrant and poorly represents strains belonging to this species. [Copyright &y& Elsevier]
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- 2010
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23. Competencies for Agricultural Advisors in Innovation Support.
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Lybaert, Charlotte, Debruyne, Lies, Kyndt, Eva, and Marchand, Fleur
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The expectation that agricultural advisors will facilitate Interactive Innovation is accompanied by novel expectations for their competency profile. In addition to their traditional technical basis, advisors are now expected to organise multi-actor processes, facilitate learning, mediate conflict, etc. Innovation support services are inherently diverse. To date, no precise list of competencies required by agricultural advisors to support Interactive Innovation has been defined. To form the basis for a competency profile, we examine the competencies currently being expected from an agricultural advisor. This suggested profile, developed in the context of the H2020 i2connect project, is based on a literature review, semi-structured interviews with co-creation experts, and an online validation workshop. We explore five themes: (a) basic disposition and attitude, (b) content competence, (c) methodological competence, (d) organisational competence, and (e) reflection, learning, and personal development. In practice, the profile can be used as either a tool for setting up co-creation processes or as the foundation for the development of new training materials. We conclude with a recommendation to create teams of advisors rather than relying on individuals, as a team is more likely to comprise the diversity of required competencies. [ABSTRACT FROM AUTHOR]
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- 2022
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24. The Use of Video to Evaluate On-Farm Demonstrations as a Tactile Space for Learning.
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Cooreman, Hanne, Vandenabeele, Joke, Debruyne, Lies, and Marchand, Fleur
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Tactile spaces as learning environments influence individuals' attitudes through social embeddedness or interconnections among people, and physical embodiedness through experiencing surroundings, potentially fostering deep commitments. When on-farm demonstrations operate as tactile spaces, they could potentially support the adoption of innovative agricultural practices. In this article, we introduce video analysis as a methodological approach to evaluate this potential of on-farm demonstration (OFD) as tactile spaces. We reflect upon this methodology with a lens on three Belgian on-farm demonstrations, each on a different topic with a different participant group, all including farmers. As a first result, this method assists in defining strengths and weaknesses of an OFD in terms of using its potential as a rich learning environment. Based on our cases, we suggest deliberately incorporating physical interaction opportunities and verbal references to the surroundings during OFDs, as our data reveals that physical embodiedness opportunities stimulate verbal and physical interactions. However, more research should confirm this. Secondly, our research resulted in lessons learned for future use of video to evaluate OFDs as tactile spaces, building on the VDA methodological framework of Nassauer and Legewie (2018). We summarise our insights in methodological guidelines, which can serve as a starting point to guide future research. [ABSTRACT FROM AUTHOR]
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- 2020
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25. Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms.
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De Smet, Sarah, De Zutter, Lieven, Debruyne, Lies, Vangroenweghe, Frédéric, Vandamme, Peter, and Houf, Kurt
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POPULATION dynamics , *SWINE infections , *PATHOGENIC microorganisms , *FECES examination , *MICROBIOLOGY , *FECES , *POLYMERASE chain reaction , *GENETIC polymorphisms , *SWINE farrowing facilities - Abstract
Healthy pigs are an important reservoir for the emerging human pathogen Arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. Up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. The present study aimed to elucidate the Arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. On each farm, 30 clinically healthy, 12-week-old piglets were selected. Fecal samples were collected on 10 sampling occasions until a slaughter age of 30 weeks was reached. Arcobacter spp. were isolated by a selective method and identified by multiplex PCR. The genetic diversity was examined by amplified fragment length polymorphism and enterobacterial repetitive intergenic consensus PCR. The Arcobacter presence in the fecal samples on the four farms ranged from 113 to 50.0%, with excretion levels of up to iO CFU/g feces. The ratio in which Arcobacter species were isolated varied between the farms and over time. Characterization revealed a high degree of genotypic diversity among the isolates. Arcobacter strains persisted and spread within the finishing unit during the fattening period. The occurrence of both unique and shared genotypes in pigs in adjacent and nonadjacent pens demonstrates that transmission routes other than fecal-oral transmission occur. [ABSTRACT FROM AUTHOR]
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- 2011
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26. Campylobacter troglodytis sp. nov., Isolated from Feces of Human-Habituated Wild Chimpanzees (Pan troglodytes schweinfurthii) in Tanzania.
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Kaur, Taranjit, Singh, Jatinder, Huffman, Michael A., Petrželková, Klára J., Taylor, Nancy S., Shilu Xu, Dewhirst, Floyd E., Paster, Bruce J., Debruyne, Lies, Vandamme, Peter, and Fox, James G.
- Subjects
- *
CHIMPANZEES , *CAVE dwellers , *CAMPYLOBACTER , *NUCLEIC acid hybridization , *ANIMAL genetics , *ANIMAL behavior genetics , *HEALTH - Abstract
The transmission of simian immunodeficiency and Ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. In this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (Pan troglodytes) populations with different histories in relation to their proximity to humans. Campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research and tourism purposes at Mahale Mountains National Park) and 0/15 (0%) group 2 (not human habituated but propagated from an introduced population released from captivity over 30 years ago at Rubondo Island National Park) chimpanzees, respectively. Using 16S rRNA gene sequencing, all isolates were virtually identical (at most a single base difference), and the chimpanzee isolates were most closely related to Campylobacter helveticus and Campylobacter upsaliensis (94.7% and 95.9% similarity, respectively). Whole- cell protein profiling, amplified fragment length polymorphism analysis of genomic DNA, hsp60 sequence analysis, and determination of the mol% G+C content revealed two subgroups among the chimpanzee isolates. DNA-DNA hybridization experiments confirmed that both subgroups represented distinct genomic species. In the absence of differential biochemical characteristics and morphology and identical 16S rRNA gene sequences, we propose to classify all isolates into a single novel nomenspecies, Campylobader troglodytis, with strain MIT 05-9149 as the type strain; strain MIT 05-9157 is suggested as the reference strain for the second C. troglodytis genomovar. Further studies are required to determine whether the organism is pathogenic to chimpanzees and whether this novel Campylobacter colonizes humans and causes enteric disease. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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