36 results on '"Demetz, Egon"'
Search Results
2. Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification
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Gollmann-Tepeköylü, Can, Graber, Michael, Hirsch, Jakob, Mair, Sophia, Naschberger, Andreas, Pölzl, Leo, Nägele, Felix, Kirchmair, Elke, Degenhart, Gerald, Demetz, Egon, Hilbe, Richard, Chen, Hao-Yu, Engert, James C., Böhm, Anna, Franz, Nadja, Lobenwein, Daniela, Lener, Daniela, Fuchs, Christiane, Weihs, Anna, Töchterle, Sonja, Vogel, Georg F., Schweiger, Victor, Eder, Jonas, Pietschmann, Peter, Seifert, Markus, Kronenberg, Florian, Coassin, Stefan, Blumer, Michael, Hackl, Hubert, Meyer, Dirk, Feuchtner, Gudrun, Kirchmair, Rudolf, Troppmair, Jakob, Krane, Markus, Weiss, Günther, Tsimikas, Sotirios, Thanassoulis, George, Grimm, Michael, Rupp, Bernhard, Huber, Lukas A., Zhang, Shen-Ying, Casanova, Jean-Laurent, Tancevski, Ivan, and Holfeld, Johannes
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- 2023
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3. Momelotinib inhibits ACVR1/ALK2, decreases hepcidin production, and ameliorates anemia of chronic disease in rodents
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Asshoff, Malte, Petzer, Verena, Warr, Matthew R., Haschka, David, Tymoszuk, Piotr, Demetz, Egon, Seifert, Markus, Posch, Wilfried, Nairz, Manfred, Maciejewski, Pat, Fowles, Peter, Burns, Christopher J., Smith, Gregg, Wagner, Kay-Uwe, Weiss, Guenter, Whitney, J.Andrew, and Theurl, Igor
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- 2017
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4. The negative adipogenesis regulator Dlk1 is transcriptionally regulated by Ifrd1 (TIS7) and translationally by its orthologue Ifrd2 (SKMc15).
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Vietor, Ilja, Cikes, Domagoj, Piironen, Kati, Vasakou, Theodora, Heimdörfer, David, Gstir, Ronald, Erlacher, Matthias David, Tancevski, Ivan, Eller, Philipp, Demetz, Egon, Hess, Michael W., Kuhn, Volker, Degenhart, Gerald, Rozman, Jan, Klingenspor, Martin, de Angelis, Martin Hrabe, Valovka, Taras, and Huber, Lukas A
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- 2023
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5. Mycobacterial heat shock protein 65 (mbHSP65)-induced atherosclerosis: Preventive oral tolerization and definition of atheroprotective and atherogenic mbHSP65 peptides
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Grundtman, Cecilia, Jakic, Bojana, Buszko, Maja, Onestingel, Elisabeth, Almanzar, Giovanni, Demetz, Egon, Dietrich, Hermann, Cappellano, Giuseppe, and Wick, Georg
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- 2015
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6. ‘Ride on the ferrous wheel’ – The cycle of iron in macrophages in health and disease
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Nairz, Manfred, Schroll, Andrea, Demetz, Egon, Tancevski, Ivan, Theurl, Igor, and Weiss, Günter
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- 2015
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7. The Arachidonic Acid Metabolome Serves as a Conserved Regulator of Cholesterol Metabolism
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Demetz, Egon, Schroll, Andrea, Auer, Kristina, Heim, Christiane, Patsch, Josef R., Eller, Philipp, Theurl, Markus, Theurl, Igor, Theurl, Milan, Seifert, Markus, Lener, Daniela, Stanzl, Ursula, Haschka, David, Asshoff, Malte, Dichtl, Stefanie, Nairz, Manfred, Huber, Eva, Stadlinger, Martin, Moschen, Alexander R., Li, Xiaorong, Pallweber, Petra, Scharnagl, Hubert, Stojakovic, Tatjana, März, Winfried, Kleber, Marcus E., Garlaschelli, Katia, Uboldi, Patrizia, Catapano, Alberico L., Stellaard, Frans, Rudling, Mats, Kuba, Keiji, Imai, Yumiko, Arita, Makoto, Schuetz, John D., Pramstaller, Peter P., Tietge, Uwe J.F., Trauner, Michael, Norata, Giuseppe D., Claudel, Thierry, Hicks, Andrew A., Weiss, Guenter, and Tancevski, Ivan
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- 2014
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8. Secretoneurin gene therapy improves hind limb and cardiac ischaemia in Apo E−/− mice without influencing systemic atherosclerosis
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Theurl, Markus, Schgoer, Wilfried, Albrecht-Schgoer, Karin, Lener, Daniela, Wolf, Dominik, Wolf, Maria, Demetz, Egon, Tymoszuk, Piotr, Tancevski, Ivan, Fischer-Colbrie, Reiner, Franz, Wolfgang-Michael, Marschang, Peter, and Kirchmair, Rudolf
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- 2015
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9. Hypoxia induced downregulation of hepcidin is mediated by platelet derived growth factor BB
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Sonnweber, Thomas, Nachbaur, David, Schroll, Andrea, Nairz, Manfred, Seifert, Markus, Demetz, Egon, Haschka, David, Mitterstiller, Anna-Maria, Kleinsasser, Axel, Burtscher, Martin, Trübsbach, Susanne, Murphy, Anthony T, Wroblewski, Victor, Witcher, Derrick R, Mleczko-Sanecka, Katarzyna, Vecchi, Chiara, Muckenthaler, Martina U, Pietrangelo, Antonello, Theurl, Igor, and Weiss, Günter
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- 2014
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10. Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2
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Tancevski, Ivan, Nairz, Manfred, Duwensee, Kristina, Auer, Kristina, Schroll, Andrea, Heim, Christiane, Feistritzer, Clemens, Hoefer, Julia, Gerner, Romana R, Moschen, Alexander R, Heller, Ingrid, Pallweber, Petra, Li, Xiaorong, Theurl, Markus, Demetz, Egon, Wolf, Anna M, Wolf, Dominik, Eller, Philipp, Ritsch, Andreas, and Weiss, Guenter
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- 2014
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11. DMT1 Protects Macrophages from Salmonella Infection by Controlling Cellular Iron Turnover and Lipocalin 2 Expression.
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Grander, Manuel, Hoffmann, Alexander, Seifert, Markus, Demetz, Egon, Grubwieser, Philipp, Pfeifhofer-Obermair, Christa, Haschka, David, and Weiss, Günter
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SALMONELLA diseases ,FERRITIN ,INFECTION control ,MACROPHAGES ,TRANSFERRIN receptors ,IRON ,SALMONELLA typhimurium - Abstract
Macrophages are at the center of innate pathogen control and iron recycling. Divalent metal transporter 1 (DMT1) is essential for the uptake of non-transferrin-bound iron (NTBI) into macrophages and for the transfer of transferrin-bound iron from the endosome to the cytoplasm. As the control of cellular iron trafficking is central for the control of infection with siderophilic pathogens such as Salmonella Typhimurium, a Gram-negative bacterium residing within the phagosome of macrophages, we examined the potential role of DMT1 for infection control. Bone marrow derived macrophages lacking DMT1 (DMT1fl/fl
LysMCre (+) ) present with reduced NTBI uptake and reduced levels of the iron storage protein ferritin, the iron exporter ferroportin and, surprisingly, of the iron uptake protein transferrin receptor. Further, DMT1-deficient macrophages have an impaired control of Salmonella Typhimurium infection, paralleled by reduced levels of the peptide lipocalin-2 (LCN2). LCN2 exerts anti-bacterial activity upon binding of microbial siderophores but also facilitates systemic and cellular hypoferremia. Remarkably, nifedipine, a pharmacological DMT1 activator, stimulates LCN2 expression in RAW264.7 macrophages, confirming its DMT1-dependent regulation. In addition, the absence of DMT1 increases the availability of iron for Salmonella upon infection and leads to increased bacterial proliferation and persistence within macrophages. Accordingly, mice harboring a macrophage-selective DMT1 disruption demonstrate reduced survival following Salmonella infection. This study highlights the importance of DMT1 in nutritional immunity and the significance of iron delivery for the control of infection with siderophilic bacteria. [ABSTRACT FROM AUTHOR]- Published
- 2022
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12. The K121Q polymorphism of ENPP1 and peripheral arterial disease
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Eller, Philipp, Schgoer, Wilfried, Mueller, Thomas, Tancevski, Ivan, Demetz, Egon, Duwensee, Kristina, Ritsch, Andreas, Haltmayer, Meinhard, and Patsch, Josef R.
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- 2008
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13. Mitochondrial Respiration in Response to Iron Deficiency Anemia: Comparison of Peripheral Blood Mononuclear Cells and Liver.
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Fischer, Christine, Valente de Souza, Lara, Komlódi, Timea, Garcia-Souza, Luiz F., Volani, Chiara, Tymoszuk, Piotr, Demetz, Egon, Seifert, Markus, Auer, Kristina, Hilbe, Richard, Brigo, Natascha, Petzer, Verena, Asshoff, Malte, Gnaiger, Erich, and Weiss, Günter
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IRON deficiency anemia ,LIVER cells ,RESPIRATION ,MITOCHONDRIA ,CITRATE synthase ,MONONUCLEAR leukocytes ,IRON deficiency - Abstract
Iron is an essential component for metabolic processes, including oxygen transport within hemoglobin, tricarboxylic acid (TCA) cycle activity, and mitochondrial energy transformation. Iron deficiency can thus lead to metabolic dysfunction and eventually result in iron deficiency anemia (IDA), which affects approximately 1.5 billion people worldwide. Using a rat model of IDA induced by phlebotomy, we studied the effects of IDA on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the liver. Furthermore, we evaluated whether the mitochondrial function evaluated by high-resolution respirometry in PBMCs reflects corresponding alterations in the liver. Surprisingly, mitochondrial respiratory capacity was increased in PBMCs from rats with IDA compared to the controls. In contrast, mitochondrial respiration remained unaffected in livers from IDA rats. Of note, citrate synthase activity indicated an increased mitochondrial density in PBMCs, whereas it remained unchanged in the liver, partly explaining the different responses of mitochondrial respiration in PBMCs and the liver. Taken together, these results indicate that mitochondrial function determined in PBMCs cannot serve as a valid surrogate for respiration in the liver. Metabolic adaptions to iron deficiency resulted in different metabolic reprogramming in the blood cells and liver tissue. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Cholesteryl ester transfer protein in patients with coronary heart disease
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Duwensee, Kristina, Breitling, Lutz Philipp, Tancevski, Ivan, Rothenbacher, Dietrich, Demetz, Egon, Patsch, Josef R., Ritsch, Andreas, Eller, Philipp, and Brenner, Hermann
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- 2010
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15. The Role of Innate Immunity and Bioactive Lipid Mediators in COVID-19 and Influenza.
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Sahanic, Sabina, Löffler-Ragg, Judith, Tymoszuk, Piotr, Hilbe, Richard, Demetz, Egon, Masanetz, Rebecca K, Theurl, Markus, Holfeld, Johannes, Gollmann-Tepeköylü, Can, Tzankov, Alexandar, Weiss, Guenter, Giera, Martin, and Tancevski, Ivan
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COVID-19 ,NATURAL immunity ,VIRUS diseases ,SARS-CoV-2 ,LIPID synthesis ,INFLUENZA - Abstract
In this review, we discuss spatiotemporal kinetics and inflammatory signatures of innate immune cells specifically found in response to SARS-CoV-2 compared to influenza virus infection. Importantly, we cover the current understanding on the mechanisms by which SARS-CoV-2 may fail to engage a coordinated type I response and instead may lead to exaggerated inflammation and death. This knowledge is central for the understanding of available data on specialized pro-resolving lipid mediators in severe SARS-CoV-2 infection pointing toward inhibited E-series resolvin synthesis in severe cases. By investigating a publicly available RNA-seq database of bronchoalveolar lavage cells from patients affected by COVID-19, we moreover offer insights into the regulation of key enzymes involved in lipid mediator synthesis, critically complementing the current knowledge about the mediator lipidome in severely affected patients. This review finally discusses different potential approaches to sustain the synthesis of 3-PUFA-derived pro-resolving lipid mediators, including resolvins and lipoxins, which may critically aid in the prevention of acute lung injury and death from COVID-19. [ABSTRACT FROM AUTHOR]
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- 2021
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16. Regulation of Th1 T Cell Differentiation by Iron via Upregulation of T Cell Immunoglobulin and Mucin Containing Protein-3 (TIM-3).
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Pfeifhofer-Obermair, Christa, Tymoszuk, Piotr, Nairz, Manfred, Schroll, Andrea, Klais, Gloria, Demetz, Egon, Engl, Sabine, Brigo, Natascha, and Weiss, Günter
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T cell differentiation ,SALMONELLA enterica serovar Typhi ,TH1 cells ,T cells ,T helper cells ,SALMONELLA enterica serovar typhimurium - Abstract
Iron plays an important role in host–pathogen interactions, in being an essential element for both pathogen and host metabolism, but also by impacting immune cell differentiation and anti-microbial effector pathways. Iron has been implicated to affect the differentiation of T lymphocytes during inflammation, however, so far the underlying mechanism remained elusive. In order to study the role of iron in T cell differentiation we here investigated how dietary iron supplementation affects T cell function and outcome in a model of chronic infection with the intracellular bacterium Salmonella enterica serovar typhimurium (S. Typhimurium). Iron loading prior to infection fostered bacterial burden and, unexpectedly, reduced differentiation of CD4
+ T helper cells type 1 (Th1) and expression of interferon-gamma (IFNγ), a key cytokine to control infections with intracellular pathogens. This effect could be traced back to iron-mediated induction of the negative immune checkpoint regulator T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3), expressed on the surface of this T cell subset. In vitro experiments demonstrated that iron supplementation specifically upregulated mRNA and protein expression of TIM-3 in naïve Th cells in a dose-depdendent manner and hindered priming of those T cells towards Th1 differentiation. Importantly, administration of TIM-3 blocking antibodies to iron-loaded mice infected with S. Typhimurium virtually restored Th1 cell differentiation and significantly improved bacterial control. Our data uncover a novel mechanism by which iron modulates CD4+ cell differentiation and functionality and hence impacts infection control with intracellular pathogens. Specifically, iron inhibits the differentiation of naive CD4+ T cells to protective IFNγ producing Th1 lymphocytes via stimulation of TIM-3 expression. Finally, TIM-3 may serve as a novel drug target for the treatment of chronic infections with intracellular pathogens, specifically in iron loading diseases. [ABSTRACT FROM AUTHOR]- Published
- 2021
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17. The haemochromatosis gene Hfe and Kupffer cells control LDL cholesterol homeostasis and impact on atherosclerosis development.
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Demetz, Egon, Tymoszuk, Piotr, Hilbe, Richard, Volani, Chiara, Haschka, David, Heim, Christiane, Auer, Kristina, Lener, Daniela, Zeiger, Lucas B, Pfeifhofer-Obermair, Christa, Boehm, Anna, Obermair, Gerald J, Ablinger, Cornelia, Coassin, Stefan, Lamina, Claudia, Kager, Juliane, Petzer, Verena, Asshoff, Malte, Schroll, Andrea, and Nairz, Manfred
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Aims Imbalances of iron metabolism have been linked to the development of atherosclerosis. However, subjects with hereditary haemochromatosis have a lower prevalence of cardiovascular disease. The aim of our study was to understand the underlying mechanisms by combining data from genome-wide association study analyses in humans, CRISPR/Cas9 genome editing, and loss-of-function studies in mice. Methods and results Our analysis of the Global Lipids Genetics Consortium (GLGC) dataset revealed that single nucleotide polymorphisms (SNPs) in the haemochromatosis gene HFE associate with reduced low-density lipoprotein cholesterol (LDL-C) in human plasma. The LDL-C lowering effect could be phenocopied in dyslipidaemic ApoE
−/− mice lacking Hfe , which translated into reduced atherosclerosis burden. Mechanistically, we identified HFE as a negative regulator of LDL receptor expression in hepatocytes. Moreover, we uncovered liver-resident Kupffer cells (KCs) as central players in cholesterol homeostasis as they were found to acquire and transfer LDL-derived cholesterol to hepatocytes in an Abca1-dependent fashion, which is controlled by iron availability. Conclusion Our results disentangle novel regulatory interactions between iron metabolism, KC biology and cholesterol homeostasis which are promising targets for treating dyslipidaemia but also provide a mechanistic explanation for reduced cardiovascular morbidity in subjects with haemochromatosis. Open in new tab Download slide Open in new tab Download slide [ABSTRACT FROM AUTHOR]- Published
- 2020
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18. The Effects of Endurance Exercise and Diet on Atherosclerosis in Young and Aged ApoE-/- and Wild-Type Mice.
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Jakic, Bojana, Carlsson, Mattias, Buszko, Maja, Cappellano, Giuseppe, Onestingel, Elisabeth, Wick, Georg, Wick, Cecilia, Ploner, Christian, Foti, Maria, Hackl, Hubert, Demetz, Egon, and Dietrich, Hermann
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ATHEROSCLEROSIS ,EXERCISE ,HEAT shock proteins - Abstract
Background: Atherosclerosis is the leading cause of death worldwide. The disease development is by and large driven by old age and lifestyle factors, such as diet, physical activity, and smoking. In the present study, we have investigated the effect of exercise and diet on the development of atherosclerosis in young and aged mice.Objective: This study aimed at comparing multiple age-dependent factors that may influence atherosclerosis in a transgenic mouse model.Methods: Young (14 weeks) and aged (49-52 weeks) C57BL/6 wild-type (WT) and atherosclerosis-prone ApoE-/- mice were subjected to physical endurance exercise on a treadmill, with or without a high-fat diet. Five weeks later, the frequencies of regulatory T cells (TREGs) in lymph nodes were assessed by flow cytometry, plasmatic cytokines (interleukin [IL]-1β, IL-6, IL-10, IL-17, interferon-γ, tumor necrosis factor-α, and transforming growth factor [TGF]-β1) levels were determined by Luminex assay. Lipids (cholesterol and triglycerides) and anti-heat shock protein 60 (HSP60) autoantibodies were measured by ELISA. Aortic lesion sizes were assessed by en face imaging. Microarray analysis and qPCR of skeletal muscle gene expression were also performed.Results: Exercise leads to a reduction of aortic lesions in young ApoE-/- and aged WT mice independent of diet. In most groups, this reduction was followed by an increased proportion of TREGs and TGF-β1 levels. Moreover, gene expression analysis showed that exercise seems to affect the AMPK signaling pathway. In particular, PGC-1α1 mRNA was induced in aged WT mice, whereas it was reduced in young ApoE-/- mice. In addition, GSEA analysis showed a marked reduction in the insulin signaling pathway in aged ApoE-/- mice.Conclusion: Practicing endurance exercise seems to be enough for reducing early aortic lesion formation, independent of diet. However, this was only true in mice with smaller aortic lesions, since mice with large, advanced, complicated atherosclerotic plaques did not show any reduction in lesion size with exercise training. [ABSTRACT FROM AUTHOR]- Published
- 2018
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19. Oral Tolerization with Mycobacterial Heat Shock Protein 65 Reduces Chronic Experimental Atherosclerosis in Aged Mice.
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Onestingel, Elisabeth, Wick, Georg, Wick, Cecilia, Demetz, Egon, and Dietrich, Hermann
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AGING ,ATHEROSCLEROSIS ,HEAT shock proteins - Abstract
Background: Atherosclerosis is a chronic inflammatory disease of the artery wall where both innate and adaptive immunity play important roles. Modulation of the immune response against the stress protein antigen, heat shock protein (HSP) 60, by administration of mycobacterial HSP65 (mbHSP65) orally and/or nasally shows promising therapeutic results in young animals in the sense of less severe experimental atherosclerosis; however, the case of aged animals with already established atherosclerosis has so far never been investigated.Objective: To investigate if mbHSP65 immunization would further accelerate atherosclerotic progression in aged ApoE-/- mice (18 months old) with already long-established atherosclerosis and if these mice could be orally tolerized against mbHSP65.Methods: Aged wild-type (WT) and ApoE-/- mice (65 weeks) were immunized and/or orally treated with mbHSP65 and then either kept on normal chow or changed to high-cholesterol diet (HCD). Atherosclerosis was assessed by en face analysis and the number of CD4+CD25+FoxP3+ T regulatory cells (Tregs) was assessed by flow cytometry in lymph node and spleen cells. Total cholesterol and triglyceride levels were determined. Soluble mammalian HSP60 and anti-mouse HSP60 (mHSP60) and anti-mbHSP65 antibodies were detected by enzyme-linked immunosorbent assay.Results: As expected, aged WT mice had only minor lesions in the aorta, which did not change under HCD for 14 weeks. Aged ApoE-/- mice already had large complicated plaques, which increased in size under HCD. mbHSP65 immunization led to a significant aggravation of atherosclerosis in both WT and ApoE-/- mice irrespective of the nature of their diet. This increase was accompanied by increased titers of both anti-mHSP60 and anti-mbHSP65 antibodies in the circulation. The increased plaque formation could be significantly diminished with oral mbHSP65 tolerization. An increased number of Tregs and lower or unchanged levels of cholesterol and triglycerides were associated with the reduced size of aortal lesions.Conclusion: Oral tolerization against mbHSP65 could be used both to prevent and to treat chronic atherosclerosis in aged individuals. [ABSTRACT FROM AUTHOR]- Published
- 2017
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20. Dietary iron loading negatively affects liver mitochondrial function.
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Volani, Chiara, Doerrier, Carolina, Demetz, Egon, Haschka, David, Paglia, Giuseppe, Lavdas, Alexandros A., Gnaiger, Erich, and Weiss, Guenter
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- 2017
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21. The Role of Omega-3 Fatty Acids in Reverse Cholesterol Transport: A Review.
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Pizzini, Alex, Demetz, Egon, Hilbe, Richard, Weiss, Guenter, Tancevski, Ivan, Lunger, Lukas, and Ebenbichler, Christoph
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The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular disease have been studied extensively. However, it remains unclear to what extent n-3 PUFAs may impact Reverse Cholesterol Transport (RCT). RCT describes a mechanism by which excess cholesterol from peripheral tissues is transported to the liver for hepatobiliary excretion, thereby inhibiting foam cell formation and the development of atherosclerosis. The aim of this review is to summarize the literature and to provide an updated overview of the effects of n-3 PUFAs on key players in RCT, including apoliprotein AI (apoA-I), ATP-binding cassette transporter A1 (ABCA1), ABCG1, apoE, scavenger receptor class B type I (SR-BI), cholesteryl ester transfer protein (CETP), low-density lipoprotein receptor (LDLr), cholesterol 7 alpha-hydroxylase (CYP7A1) and ABCG5/G8. Based on current knowledge, we conclude that n-3 PUFAs may beneficially affect RCT, mainly by influencing high-density lipoprotein (HDL) remodeling and by promoting hepatobiliary sterol excretion. [ABSTRACT FROM AUTHOR]
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- 2017
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22. Heme oxygenase 1 controls early innate immune response of macrophages to Salmonella Typhimurium infection.
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Mitterstiller, Anna‐Maria, Haschka, David, Dichtl, Stefanie, Nairz, Manfred, Demetz, Egon, Talasz, Heribert, Soares, Miguel P., Einwallner, Elisa, Esterbauer, Harald, Fang, Ferric C., Geley, Stephan, and Weiss, Guenter
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SALMONELLA typhimurium ,HEME oxygenase ,NATURAL immunity ,IMMUNE response ,MACROPHAGES - Abstract
Macrophages are central for the immune control of intracellular microbes. Heme oxygenase 1 (HO-1, hmox) is the first and rate limiting enzyme in the breakdown of heme originating from degraded senescent erythrocytes and heme-proteins, yielding equal amounts of iron, carbon monoxide and biliverdin. HO-1 is strongly up-regulated in macrophages in response to inflammatory signals, including bacterial endotoxin. In view of the essential role of iron for the growth and proliferation of intracellular bacteria along with known effects of the metal on innate immune function, we examined whether HO-1 plays a role in the control of infection with the intracellular bacterium Salmonella Typhimurium. We studied the course of infection in stably-transfected murine macrophages (RAW264.7) bearing a tetracycline-inducible plasmid producing hmox shRNA and in primary HO-1 knockout macrophages. While uptake of bacteria into macrophages was not affected, a significantly reduced survival of intracellular Salmonella was observed upon hmox knockdown or pharmacological hmox inhibition, which was independent of Nramp1 functionality. This could be traced to limitation of iron availability for intramacrophage bacteria along with enhanced stimulation of innate immune effector pathways, including the formation of reactive oxygen and nitrogen species and increased TNF-α expression. Mechanistically, these latter effects result from intracellular iron limitation with subsequent activation of NF-κB and further inos, tnfa and p47phox transcription along with reduced formation of the anti-inflammatory and radical scavenging molecules, CO and biliverdin as a consequence of HO-1 silencing. Taken together our data provide novel evidence that the infection-driven induction of HO-1 exerts detrimental effects in the early control of Salmonella infection, whereas hmox inhibition can favourably modulate anti-bacterial immune effector pathways of macrophages and promote bacterial elimination. [ABSTRACT FROM AUTHOR]
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- 2016
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23. Lipocalin-2 ensures host defense against Salmonella Typhimurium by controlling macrophage iron homeostasis and immune response.
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Nairz, Manfred, Schroll, Andrea, Haschka, David, Dichtl, Stefanie, Sonnweber, Thomas, Theurl, Igor, Theurl, Milan, Lindner, Ewald, Demetz, Egon, Aßhoff, Malte, Bellmann‐Weiler, Rosa, Müller, Raphael, Gerner, Romana R., Moschen, Alexander R., Baumgartner, Nadja, Moser, Patrizia L., Talasz, Heribert, Tilg, Herbert, Fang, Ferric C., and Weiss, Günter
- Abstract
Lipocalin-2 (Lcn2) is an innate immune peptide with pleiotropic effects. Lcn2 binds iron-laden bacterial siderophores, chemo-attracts neutrophils and has immunomodulatory and apoptosis-regulating effects. In this study, we show that upon infection with Salmonella enterica serovar Typhimurium, Lcn2 promotes iron export from Salmonella-infected macrophages, which reduces cellular iron content and enhances the generation of pro-inflammatory cytokines. Lcn2 represses IL-10 production while augmenting Nos2, TNF-α, and IL-6 expression. Lcn2
−/− macrophages have elevated IL-10 levels as a consequence of increased iron content. The crucial role of Lcn-2/IL-10 interactions was further demonstrated by the greater ability of Lcn2−/− IL-10−/− macrophages and mice to control intracellular Salmonella proliferation in comparison to Lcn2−/− counterparts. Overexpression of the iron exporter ferroportin-1 in Lcn2−/− macrophages represses IL-10 and restores TNF-α and IL-6 production to the levels found in wild-type macrophages, so that killing and clearance of intracellular Salmonella is promoted. Our observations suggest that Lcn2 promotes host resistance to Salmonella Typhimurium infection by binding bacterial siderophores and suppressing IL-10 production, and that both functions are linked to its ability to shuttle iron from macrophages. [ABSTRACT FROM AUTHOR]- Published
- 2015
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24. Contrasting regulation of macrophage iron homeostasis in response to infection with Listeria monocytogenes depending on localization of bacteria.
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Haschka, David, Nairz, Manfred, Demetz, Egon, Wienerroither, Sebastian, Decker, Thomas, and Weiss, Günter
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- 2015
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25. Heme Oxygenase-1 Gene Promoter Microsatellite Polymorphism Is Associated With Progressive Atherosclerosis and Incident Cardiovascular Disease.
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Pechlaner, Raimund, Willeit, Peter, Summerer, Monika, Santer, Peter, Egger, Georg, Kronenberg, Florian, Demetz, Egon, Weiss, Günter, Tsimikas, Sotirios, Witztum, Joseph L., Willeit, Karin, Iglseder, Bernhard, Paulweber, Bernhard, Kedenko, Lyudmyla, Haun, Margot, Meisinger, Christa, Gieger, Christian, Müller-Nurasyid, Martina, Peters, Annette, and Willeit, Johann
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- 2015
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26. Iron at the interface of immunity and infection.
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Nairz, Manfred, Haschka, David, Demetz, Egon, and Weiss, Günter
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MAMMALIAN cell cycle ,IRON ,ANEMIA ,HOMEOSTASIS ,HEPCIDIN ,CYTOLOGICAL research - Abstract
Both, mammalian cells and microbes have an essential need for iron, which is required for many metabolic processes and for microbial pathogenicity. In addition, cross-regulatory interactions between iron homeostasis and immune function are evident. Cytokines and the acute phase protein hepcidin affect iron homeostasis leading to the retention of the metal within macrophages and hypoferremia. This is considered to result from a defense mechanism of the body to limit the availability of iron for extracellular pathogens while on the other hand the reduction of circulating iron results in the development of anemia of inflammation. Opposite, iron and the erythropoiesis inducing hormone erythropoietin affect innate immune responses by influencing interferon-gamma (IFN-y) mediated (iron) or NF-kB inducible (erythropoietin) immune effector pathways in macrophages. Thus, macrophages loaded with iron lose their ability to kill intracellular pathogens via IFN-y mediated effector pathways such as nitric oxide (NO) formation. Accordingly, macrophages invaded by the intracellular bacterium Salmonella enterica serovar Typhimurium increase the expression of the iron export protein ferroportin thereby reducing the availability of iron for intramacrophage bacteria while on the other side strengthening anti-microbial macrophage effector pathways via increased formation of NO orTNF-a. In addition, certain innate resistance genes such as natural resistance associated macrophage protein function (Nramp1) or lipocalin-2 exert part of their antimicrobial activity by controlling host and/or microbial iron homeostasis. Consequently, pharmacological or dietary modification of cellular iron trafficking enhances host resistance to intracellular pathogens but may increase susceptibility to microbes in the extracellular compartment and vice versa. Thus, the control over iron homeostasis is a central battlefield in host-pathogen interplay influencing the course of an infectious disease in favor of either the mammalian host or the pathogenic invader. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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27. Haptoglobin 2-2 Genotype is Not Associated With Cardiovascular Risk in Subjects With Elevated Glycohemoglobin—Results From the Bruneck Study.
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Pechlaner, Raimund, Kiechl, Stefan, Willeit, Peter, Demetz, Egon, Haun, Margot, Weger, Siegfried, Oberhollenzer, Friedrich, Kronenberg, Florian, Bonora, Enzo, Weiss, Günter, and Willeit, Johann
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- 2014
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28. Inhibition of hepatic scavenger receptor-class B type I by RNA interference decreases atherosclerosis in rabbits
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Demetz, Egon, Tancevski, Ivan, Duwensee, Kristina, Stanzl, Ursula, Huber, Eva, Heim, Christiane, Handle, Florian, Theurl, Markus, Schroll, Andrea, Tailleux, Anne, Dietrich, Hermann, Patsch, Josef R., Eller, Philipp, and Ritsch, Andreas
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SCAVENGER receptors (Biochemistry) , *RNA interference , *ATHEROSCLEROSIS , *HIGH density lipoproteins , *HOMEOSTASIS , *MESSENGER RNA , *LABORATORY rabbits - Abstract
Abstract: Objective: Scavenger receptor-class B type I (SR-BI), the receptor for HDL-cholesterol, plays a key role in HDL metabolism, whole body cholesterol homeostasis, and reverse cholesterol transport. We investigated the in vivo impact of hepatic SR-BI inhibition on lipoprotein metabolism and the development of atherosclerosis employing RNA interference. Methods: Small hairpin RNA plasmid specific for rabbit SR-BI was complexed with galactosylated poly-l-lysine, allowing an organ-selective, receptor-mediated gene transfer. Rabbits were fed a cholesterol-rich diet, and were injected with plasmid-complexes once a week. Results: After 2 weeks of treatment hepatic SR-BI mRNA levels were reduced by 80% accompanied by reduced SR-BI protein levels and a modulation of the lipoprotein profile. Rabbits treated with SR-BI-specific plasmid-complexes displayed higher cholesteryl ester transfer from HDL to apoB-containing lipoproteins, lower HDL-cholesterol, and higher VLDL-cholesterol levels, when compared to controls. In a long-term study, this gene therapeutic intervention led to a similar modulation of the lipoprotein profile, to lower total cholesterol levels, and most importantly to a 50% reduction of the relative atherosclerotic lesion area. Conclusion: Our results are another indication that the role of SR-BI in lipoprotein metabolism and atherogenesis in rabbits – a CETP-expressing animal model displaying a manlike lipoprotein profile may be different from the one found in rodents. [Copyright &y& Elsevier]
- Published
- 2012
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29. Cholesteryl ester transfer protein and mortality in patients undergoing coronary angiography: the Ludwigshafen Risk and Cardiovascular Health study.
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Ritsch A, Scharnagl H, Eller P, Tancevski I, Duwensee K, Demetz E, Sandhofer A, Boehm BO, Winkelmann BR, Patsch JR, März W, Ritsch, Andreas, Scharnagl, Hubert, Eller, Philipp, Tancevski, Ivan, Duwensee, Kristina, Demetz, Egon, Sandhofer, Anton, Boehm, Bernhard O, and Winkelmann, Bernhard R
- Published
- 2010
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30. The Liver-Selective Thyromimetic T-0681 Influences Reverse Cholesterol Transport and Atherosclerosis Development in Mice.
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Tancevski, Ivan, Demetz, Egon, Eller, Philipp, Duwensee, Kristina, Hoefer, Julia, Heim, Christiane, Stanzl, Ursula, Wehinger, Andreas, Auer, Kristina, Karer, Regina, Huber, Julia, Schgoer, Wilfried, Van Eck, Miranda, Vanhoutte, Jonathan, Fievet, Catherine, Stellaard, Frans, Rudling, Mats, Patsch, Josef R., and Ritsch, Andreas
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LIPOPROTEINS , *MACROPHAGES , *BLOOD plasma , *STEROLS , *LIPIDS , *PROTEINS , *BLOOD lipoproteins , *ANTIGEN presenting cells , *CONNECTIVE tissue cells - Abstract
Background: Liver-selective thyromimetics have been reported to efficiently reduce plasma cholesterol through the hepatic induction of both, the low-density lipoprotein receptor (LDLr) and the high-density lipoprotein (HDL) receptor; the scavenger receptor class B type I (SR-BI). Here, we investigated the effect of the thyromimetic T-0681 on reverse cholesterol transport (RCT) and atherosclerosis, and studied the underlying mechanisms using different mouse models, including mice lacking LDLr, SR-BI, and apoE, as well as CETP transgenic mice. Methodology/Principal Findings: T-0681 treatment promoted bile acid production and biliary sterol secretion consistently in the majority of the studied mouse models, which was associated with a marked reduction of plasma cholesterol. Using an assay of macrophage RCT in mice, we found T-0681 to significantly increase fecal excretion of macrophage-derived neutral and acidic sterols. No positive effect on RCT was found in CETP transgenic mice, most likely due to the observed decrease in plasma CETP mass. Studies in SR-BI KO and LDLr KO mice suggested hepatic LDLr to be necessary for the action of T-0681 on lipid metabolism, as the compound did not have any influence on plasma cholesterol levels in mice lacking this receptor. Finally, prolonged treatment with T-0681 reduced the development of atherosclerosis by 60% in apoE KOs on Western type diet. In contrast, at an earlier time-point T-0681 slightly increased small fatty streak lesions, in part due to an impaired macrophage cholesterol efflux capacity, when compared to controls. Conclusions/Significance: The present results show that liver-selective thyromimetics can promote RCT and that such compounds may protect from atherosclerosis partly through induction of bile acid metabolism and biliary sterol secretion. On-going clinical trials will show whether selective thyromimetics do prevent atherosclerosis also in humans. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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31. Dietary Iron Overload and Hfe −/− Related Hemochromatosis Alter Hepatic Mitochondrial Function.
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Fischer, Christine, Volani, Chiara, Komlódi, Timea, Seifert, Markus, Demetz, Egon, Valente de Souza, Lara, Auer, Kristina, Petzer, Verena, von Raffay, Laura, Moser, Patrizia, Gnaiger, Erich, and Weiss, Guenter
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IRON overload ,HEMOCHROMATOSIS ,MITOCHONDRIA ,REACTIVE oxygen species ,GENETIC load ,TRANSFERRIN - Abstract
Iron is an essential co-factor for many cellular metabolic processes, and mitochondria are main sites of utilization. Iron accumulation promotes production of reactive oxygen species (ROS) via the catalytic activity of iron species. Herein, we investigated the consequences of dietary and genetic iron overload on mitochondrial function. C57BL/6N wildtype and Hfe
−/− mice, the latter a genetic hemochromatosis model, received either normal diet (ND) or high iron diet (HI) for two weeks. Liver mitochondrial respiration was measured using high-resolution respirometry along with analysis of expression of specific proteins and ROS production. HI promoted tissue iron accumulation and slightly affected mitochondrial function in wildtype mice. Hepatic mitochondrial function was impaired in Hfe−/− mice on ND and HI. Compared to wildtype mice, Hfe−/− mice on ND showed increased mitochondrial respiratory capacity. Hfe−/− mice on HI showed very high liver iron levels, decreased mitochondrial respiratory capacity and increased ROS production associated with reduced mitochondrial aconitase activity. Although Hfe−/− resulted in increased mitochondrial iron loading, the concentration of metabolically reactive cytoplasmic iron and mitochondrial density remained unchanged. Our data show multiple effects of dietary and genetic iron loading on mitochondrial function and linked metabolic pathways, providing an explanation for fatigue in iron-overloaded hemochromatosis patients, and suggests iron reduction therapy for improvement of mitochondrial function. [ABSTRACT FROM AUTHOR]- Published
- 2021
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32. Cytokine-Mediated Regulation of ARG1 in Macrophages and Its Impact on the Control of Salmonella enterica Serovar Typhimurium Infection.
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Brigo, Natascha, Pfeifhofer-Obermair, Christa, Tymoszuk, Piotr, Demetz, Egon, Engl, Sabine, Barros-Pinkelnig, Marina, Dichtl, Stefanie, Fischer, Christine, Valente De Souza, Lara, Petzer, Verena, von Raffay, Laura, Hilbe, Richard, Berger, Sylvia, Seifert, Markus, Schleicher, Ulrike, Bogdan, Christian, and Weiss, Günter
- Subjects
SALMONELLA enterica serovar typhimurium ,SALMONELLA enterica ,SALMONELLA diseases ,NITRIC-oxide synthases ,MACROPHAGES ,BACTERIAL metabolism ,SALMONELLA enterica serovar Typhi ,INTRACELLULAR pathogens - Abstract
Arginase 1 (ARG1) is a cytosolic enzyme that cleaves L-arginine, the substrate of inducible nitric oxide synthase (iNOS), and thereby impairs the control of various intracellular pathogens. Herein, we investigated the role of ARG1 during infection with Salmonella enterica serovar Typhimurium (S.tm). To study the impact of ARG1 on Salmonella infections in vitro, bone marrow-derived macrophages (BMDM) from C57BL/6N wild-type, ARG1-deficient Tie2Cre
+/− ARG1fl/fl and NRAMPG169 C57BL/6N mice were infected with S.tm. In wild-type BMDM, ARG1 was induced by S.tm and further upregulated by the addition of interleukin (IL)-4, whereas interferon-γ had an inhibitory effect. Deletion of ARG1 did not result in a reduction in bacterial numbers. In vivo, Arg1 mRNA was upregulated in the spleen, but not in the liver of C57BL/6N mice following intraperitoneal S.tm infection. The genetic deletion of ARG1 (Tie2Cre+/− ARG1fl/fl ) or its pharmacological inhibition with CB-1158 neither affected the numbers of S.tm in spleen, liver and blood nor the expression of host response genes such as iNOS, IL-6 or tumour necrosis factor (TNF). Furthermore, ARG1 was dispensable for pathogen control irrespective of the presence or absence of the phagolysosomal natural resistance-associated macrophage protein 1 (NRAMP1). Thus, unlike the detrimental function of ARG1 seen during infections with other intraphagosomal microorganisms, ARG1 did not support bacterial survival in systemic salmonellosis, indicating differential roles of arginine metabolism for host immune response and microbe persistence depending on the type of pathogen. [ABSTRACT FROM AUTHOR]- Published
- 2021
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33. Metabolic Signature of Dietary Iron Overload in a Mouse Model.
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Volani, Chiara, Paglia, Giuseppe, Smarason, Sigurdur V., Pramstaller, Peter P., Demetz, Egon, Pfeifhofer-Obermair, Christa, and Weiss, Guenter
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COFACTORS (Biochemistry) ,OXIDATIVE phosphorylation ,IRON in the body ,LABORATORY mice ,HOMEOSTASIS ,KREBS cycle - Abstract
Iron is an essential co-factor for several metabolic processes, including the Krebs cycle and mitochondrial oxidative phosphorylation. Therefore, maintaining an appropriate iron balance is essential to ensure sufficient energy production and to avoid excessive reactive oxygen species formation. Iron overload impairs mitochondrial fitness; however, little is known about the associated metabolic changes. Here we aimed to characterize the metabolic signature triggered by dietary iron overload over time in a mouse model, where mice received either a standard or a high-iron diet. Metabolic profiling was assessed in blood, plasma and liver tissue. Peripheral blood was collected by means of volumetric absorptive microsampling (VAMS). Extracted blood and tissue metabolites were analyzed by liquid chromatography combined to high resolution mass spectrometry. Upon dietary iron loading we found increased glucose, aspartic acid and 2-/3-hydroxybutyric acid levels but low lactate and malate levels in peripheral blood and plasma, pointing to a re-programming of glucose homeostasis and the Krebs cycle. Further, iron loading resulted in the stimulation of the urea cycle in the liver. In addition, oxidative stress was enhanced in circulation and coincided with increased liver glutathione and systemic cysteine synthesis. Overall, iron supplementation affected several central metabolic circuits over time. Hence, in vivo investigation of metabolic signatures represents a novel and useful tool for getting deeper insights into iron-dependent regulatory circuits and for monitoring of patients with primary and secondary iron overload, and those ones receiving iron supplementation therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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34. The PIDDosome activates p53 in response to supernumerary centrosomes.
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Fava, Luca L., Schuler, Fabian, Sladky, Valentina, Haschka, Manuel D., Soratroi, Claudia, Eiterer, Lisa, Demetz, Egon, Weiss, Guenter, Geley, Stephan, Nigg, Erich A., and Villunger, Andreas
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CENTROSOMES , *CELL anatomy , *NUCLEOTIDE sequencing , *NUCLEOTIDE analysis , *NUCLEOTIDE sequence - Abstract
Centrosomes, the main microtubule-organizing centers in animal cells, are replicated exactly once during the cell division cycle to form the poles of the mitotic spindle. Supernumerary centrosomes can lead to aberrant cell division and have been causally linked to chromosomal instability and cancer. Here,we report that an increase in the number of mature centrosomes, generated by disrupting cytokinesis or forcing centrosome overduplication, triggers the activation of the PIDDosome multiprotein complex, leading to Caspase-2-mediated MDM2 cleavage, p53 stabilization, and p21-dependent cell cycle arrest. This pathway also restrains the extent of developmentally scheduled polyploidization by regulating p53 levels in hepatocytes during liver organogenesis. Taken together, the PIDDosome acts as a first barrier, engaging p53 to halt the proliferation of cells carrying more than one mature centrosome to maintain genome integrity. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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35. Identification of ALK in Thinness.
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Orthofer, Michael, Valsesia, Armand, Mägi, Reedik, Wang, Qiao-Ping, Kaczanowska, Joanna, Kozieradzki, Ivona, Leopoldi, Alexandra, Cikes, Domagoj, Zopf, Lydia M., Tretiakov, Evgenii O., Demetz, Egon, Hilbe, Richard, Boehm, Anna, Ticevic, Melita, Nõukas, Margit, Jais, Alexander, Spirk, Katrin, Clark, Teleri, Amann, Sabine, and Lepamets, Maarja
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LEANNESS , *ANAPLASTIC lymphoma kinase , *ADIPOSE tissues , *WEIGHT gain - Abstract
There is considerable inter-individual variability in susceptibility to weight gain despite an equally obesogenic environment in large parts of the world. Whereas many studies have focused on identifying the genetic susceptibility to obesity, we performed a GWAS on metabolically healthy thin individuals (lowest 6th percentile of the population-wide BMI spectrum) in a uniquely phenotyped Estonian cohort. We discovered anaplastic lymphoma kinase (ALK) as a candidate thinness gene. In Drosophila , RNAi mediated knockdown of Alk led to decreased triglyceride levels. In mice, genetic deletion of Alk resulted in thin animals with marked resistance to diet- and leptin-mutation-induced obesity. Mechanistically, we found that ALK expression in hypothalamic neurons controls energy expenditure via sympathetic control of adipose tissue lipolysis. Our genetic and mechanistic experiments identify ALK as a thinness gene, which is involved in the resistance to weight gain. • GWAS in the EGCUT biobank identifies ALK as a candidate thinness gene • Knockdown of Alk in Drosophila results in reduced triglyceride levels • Alk mutant mice exhibit resistance to diet- and leptin-mutation-induced obesity • ALK controls energy expenditure via sympathetic tone to the adipose organ Genetic association studies in an Estonian biobank implicate ALK in the regulation of thinness. Studies in Drosophila and mice show that ALK functions as a regulator of sympathetic tone and loss of ALK leads to resistance to weight gain. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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36. Mycobacterial heat shock protein 65 (mbHSP65)-induced atherosclerosis - Preventive oral tolerization and definition of atheroprotective and atherogenic mbHSP65-peptides.
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Grundtman, Cecilia, Onestingel, Elisabeth, Jakic, Bojana, Buszko, Maja A., Almanzar, Giovanni, Demetz, Egon, Dietrich, Hermann, and Wick, Georg
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ATHEROSCLEROSIS , *HEAT shock proteins , *CLINICAL immunology , *IMMUNE response , *CYTOKINES , *DISEASE progression - Published
- 2017
- Full Text
- View/download PDF
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