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2. Author Correction: Detection of urinary podocytes by flow cytometry in idiopathic membranous nephropathy

Author

Roberta Clari, Silvia Mingozzi, Caterina Dolla, Ilaria Deambrosis, Stefania Bruno, Giovanni Camussi, Luigi Biancone, Fulvia Giaretta, Manuel Burdese, Elena Boaglio, Loredana Colla, and Alberto Mella

Subjects
Adult, Male, Pathology, medicine.medical_specialty, Urinary system, Science, Glomerulonephritis, Membranous, Flow cytometry, Humans, Medicine, Prospective Studies, Author Correction, Aged, Autoantibodies, Multidisciplinary, medicine.diagnostic_test, Podocytes, business.industry, Receptors, Phospholipase A2, Middle Aged, Flow Cytometry, Prognosis, Idiopathic Membranous Nephropathy, Case-Control Studies, Female, business, Biomarkers
Abstract

Idiopathic membranous nephropathy (iMN) is considered an immune-mediated disease where circulating autoantibodies against podocyte targets (mainly the PLA

Published
2020

3. Transplantation - clinical II

Author

Handan Ozdemir, Himanshu V Patel, Tingli Wang, Izumi Yamamoto, Anna Perri, Eva Svobodova, Alberto Mella, Alexander Kildushevsky, Ole Øyen, Nicolas Congy-Jolivet, Sergio Luis-Lima, Yuangao Zou, Hideki Fuji, Tolga Yildirim, Norihiko Goto, Marian Klinger, Leonídio Dias, Won Seok Yang, Lanlan Wang, Tatjana Cvetkovic, Barbara Assenzio, Filiz Bakar, Samantha Mantovani, Halil Yazici, Vijay Thanaraj, Priyadarshini S Shah, Solbjørg Sagedal, Francesca Sidoti, Anupma Kaul, Giordano Zampi, Dharmendra Bhadauria, Moncef Mokni, Tatjana Jevtovic Stoimenov, Tine Thurison, Alessia Di Nauta, Christos S. Katsouras, Franca Sinesi, Denisa Mendonça, Jude Yagan, Atsushi Miki, Giorgos Spanos, F. Zalamea Jarrin, Maria Cristina Di Vico, Francesca Greco, Marco Ballestri, Veena R Shah, Takashi Yokoo, António Castro Henriques, Pranjal R Modi, Vito Fanelli, Andrey Vatazin, Jiri Fronek, Siren Sezer, Anna Teresa Mazzeo, Takayuki Yamamoto, Karen Stopper, Jerzy Chudek, Antonij Slavcev, Deepak Shankar Ray, Takaaki Kimura, Claudio Musetti, Christina Dörje, Lidija Orlić, Osman Ilhan, Rahmi Yilmaz, Francesca Damiano, Mehmet Gokhan Caglayan, Marcel Naik, Ken Kitamura, Maarten B. Rookmaaker, Arjan D. van Zuilen, Pablo Raffaele, Trond Jenssen, Yosu Luque, Masato Fujisawa, Katerina K. Naka, Giuseppe Paolo Segoloni, Ioannis Gkirdis, Leonardo Caroti, Maarten Naesens, Klemens Budde, Rossana Cavallo, Nanna von der Lippe, Ilaria Mastromauro, A. Azzebi, Rigas Kalaitzidis, Fredrik B. Brekke, António Cabrita, Sławomir Zmonarski, Flavio Vincenti, Alessandro De Vincenzi, Hela Ghezaiel, Vasilis Koutlas, Elena Cremaschi, Dorota Kamińska, Mark A. J. Devonald, Kostas C. Siamopoulos, Mahmut Altindal, Mehtap Ekmen Uyar, Dobrin Svinarov, Abdulrahman Housawi, Sanjin Rački, Wissal Sahtout, Ramazan Cetinkaya, Emre Tutal, Luísa Lobato, Mehmet Sukru Sever, Umberto Maggiore, Momir Mikov, Dorry L. Segev, Inés Beired Val, Stefania Bussolino, Fernando Gil Catalinas, Steffen Thiel, Anna Kotsia, Olesja Rissling, Halil Ermis, Borelli K. Zlatkov, Adam Varga, Efrat Harel, Eva Pokorna, P. Chudoba, Saliha Uyanık, Kostas Pappas, Maria Vittoria Mauro, Viktorija Dragojevic-Simic, Alexey Zulkarnaev, Vural Taner Yilmaz, Mikiko Yoshikawa, Luciana Mascia, Aureliusz Kolonko, Yasunaru Sakuma, Manoj R Gumber, Jens Bollerslev, Maria Boratyńska, Kathy Denesyk, Lionel Rostaing, Fabrizio Fop, Carlo Massimetti, Tak Mao Chan, Jamal Rizvi, Predrag Vlahovic, Anna Maria Degli Antoni, Yasuo Takeuchi, Bård Waldum, Pieter Evenepoel, Dai Kohguchi, Aurelio Rodríguez-Hernández, Marcin Protasiewicz, Yudo Tannno, Samira Ben Amor, Sofia Pedroso, Rusudana Kantaria, Nikola Stefanović, Renzo Bonofilgio, Kristin Godang, Hans-H. Neumayer, Aydin Turkmen, Bosiljka Devcic, Oktawia Mazanowska, Tomasz Dawiskiba, Zeynep Bal, Donatella Vizza, J. Portoles Perez, Maggie Ming Yee Mok, Veronika Fedulkina, Josefina Santos, Byung Ha Chung, Altagracia Bello Ovalle, Lampros Lakkas, Jean-Luc Taupin, Anis Belarbia, Anil Chandraker, Andrea Ranghino, Sharmas Vali, Yaeni Kim, Yong-Soo Kim, Dorota Bartoszek, Sakuma Yasunaru, Mirosław Banasik, Malgorzata Kaminska, Cheol Whee Park, Jonathan Visentin, Fulvia Giaretta, Nobuo Tsuboi, Nathan T. James, Tiziana Cena, Tri Q. Nquyen, Giovanni Piotti, Huseyin Kocak, Armando Torres, Milagros Sierra Carpio, Gabriela Pimentel Guzmán, O. Lafuente Covarrubias, B. Sanchez Sobrino, Yuliya V Smedbraaten, Morten W. Fagerland, Pankaj R Shah, Amin Amro, Maria Granito, Davide Diena, Hargovind L Trivedi, Kenan Keven, Hyuk Y Kwon, Bei Cai, Alena Parikova, Ercan Turkmen, Jean J. Filipov, Marc-Olivier Timsit, Alastair Ferraro, Nicoline M.H. Veldhuijzen, Ibrahim Aliosmanoglu, Koji Nanmoku, R K Sharma, Neven Vavic, Anders Hartmann, Nilgun Aysuna, Geir Mjøen, Barbara Sandor, Gianni Cappelli, Louise Moist, José-Carlos Oliveira, Salima Kejji, Alena Verflova, Haralampos Harisis, Babak J. Orandi, Cristina Izzo, Andras Toth, Ana González-Rinne, Ivana Mikolašević, Mehmet Haberal, Petra Reinke, Akimitsu Kobayashi, Marion Rabant, Agnieszka Sas, Giulia Ligabue, Soumava Gupta, Dmytro Khadzhynov, Soon Bae Kim, Fatih Yılmaz, Gunilla Høyer-Hansen, Fotios Zarzoulas, Semra Bozfakioglu, O. Guliyev, Mehtap Erkmen Uyar, Yasuyuki Nakada, Zeynep Kendi Celebi, Alejandro Jiménez-Sosa, Christophe Legendre, Haralampos Pappas, José Davide, Sandro Feriozzi, Keitaro Yokoyama, Mustafa Arici, Esteban Porrini, Niraj M. Desai, Dany Anglicheau, Enrico Eugenio Minetti, Shunji Narumi, Federica Civiletti, Bahar Gurlek Demirci, Pierre Merville, Pablo Klin, Natalia Negrín-Mena, Roberta Fenoglio, Pratik Das, Marco Quaglia, Abdelatif Achour, Martina Pavletic Persic, Turan Colak, Hallvard Holdaas, Hyun Seon Kim, Charlie Martinez, Nemanja Jacimovic, Sung H Son, Carlo Buzio, Francesco Fontana, Giorgos Tzeltzes, Anders Åsberg, Andrea Kantor, Sébastien Lepreux, Ana Aldea-Perona, Laure-Hélène Noël, Takafumi Yamakawa, Daniela Perugini, Magdalena Szotowska, Ichiro Ohkido, Antonio Gil Paraíso, Aruna V Vanikar, Nurhan Ozdemir Acar, Manuela Guedes de Almeida, Bo Ying Choy, Charles S. Craik, Antoine Bello, Andrzej Wiecek, Bang-Gee Hsu, Massimo Rittà, Cristina Giraldi, Björn Meijers, Gwendaline Guidicelli, Henri Kreis, Chul Woo Yang, Augusto Vaglio, Marit Elizabeth Von Düring, Kouji Nannmoku, Riccardo Magistroni, Yunus Erdem, Katrien De Vusser, Marta Artamendi Larrañaga, Hiroyasu Yamamoto, Luigi Biancone, Giorgos Nakas, Rita Marcela Fortunato, Enma Huarte Loza, Nemanja Rancic, Andrea Airoldi, Katarzyna Koscielska-Kasprzak, Francesca Leone, Kazunari Yoshida, R. Llopez Carratala, Miyeon Kim, Andrea Cossarizza, Gabriele Guglielmetti, G. Tognarelli, Burak Sayin, José Manuel González-Posada, Jin Kong, Bulent Altun, Ingrid Os, Anne Theakstone, Shantanu Bhattacharjya, Piotr Kuczera, Gian Domenico D Fabbri, Lionel Couzi, Yosra Guedri, Nurhan Ozdemir, Cristina Costa, Chung Hee Baek, Emil P. Dimitrov, Ester Gallo, Dirk Kuypers, Hermann Hernandez Vargas, Massimo Gai, Rohit Rungta, Jun-Seok Kim, Piero Stratta, Takashi Yagisawa, Cihat Burak Sayin, Antonio Amoroso, Faisal Rehman, Maria Zanazzi, P. Dominguez Apiñaniz, Teresa Papalia, Franco Brescia, Vesna Lukenda, Ruben Poesen, Gea Imperato, P. Carta, Davide Medica, Alessandra Palmisano, S. Karsten Alvarez, Rozenn Clément, Jelena Katic, F Ersoy, Vladimir Hanzal, Safa Nouira, Manisha Sahay, K. Kalmár-Nagy, Michele Battista, Eun J Whang, Kalman Toth, Andrew A. House, Anna Varberg Reisæter, Hyung Jin Cho, Evangelia Dounousi, Irini Tzalavra, Sabri Ferdaws, Gultekin Suleymanlar, Limei Luo, Ayhan Dinckan, Ilaria Deambrosis, Begum Erdemir, Akinori Nukui, Thomas Schachtner, Karsten Midtvedt, Cecilia Dall Anesse, Ewelina Sikora-Grabka, Domingo Marrero, Ming-Che Lee, Vivek B Kute, Lourdes Pérez-Tamajón, Ana Coloma Lopez, Maria Messina, Ozgur Akin Oto, Lorraine Kwan, Robert A. Montgomery, Aris Bechlioulis, Olga Balafa, Isabel Fonseca, Aida Larti, Magdalena Krajewska, Akira Kurosawa, Toshihisa Iwabuchi, Janka Slatinska, Teppei Ohyama, Agnieszka Hałoń, Daisuke Ishii, Dag Olav Dahle, Su-Kil Park, Soumaya Ben Abdelkrim, Won Y Choi, Fatma Nurhan Ozdemir Acar, Sanda Mrabet, Lorenzo Di Maria, Saliha Yildirim, Oliver Staeck, Aleksandra Kovacevic, Elisa Buti, Yasar Caliskan, Lampros K. Michalis, Makoto Tsujita, Paweł Madej, Michalis Mitsis, Eduardo Salido, Ondrej Viklicky, Kiranmai Ismal, Yoshihiko Watarai, Dimitris Evangelou, Hege Pihlstrøm, Dorsaf Zellama, Chul Soo Yoon, Radmila Veličković Radovanović, Thomas Bachelet, Duck Jong Han, Paolo Gigliotti, Shinichi Nishi, Danilo Lofaro, Nassim Kamar, Fabian Halleck, Charlotte Ng, Erik H. Strøm, Aki Mafune, Sara De Biasi, Selami Kocak Toprak, António Castro-Henriques, Fabiola Pagani, Alaattin Yildiz, Marcin Adamczak, Pablo Bridoux, Marina Colic, Anara Amanova, Kyeong Woo Nho, Anna V. Reisæter, Bum Soon Choi, Hugo Sanabria, Enrique Ramalle Gómara, Ayse Serra Artan, Walther H. Boer, Takahisa Hiramitsu, Narayan Prasad, Albane Sartorius, Juan De Francesco, Daniele Cagna, Jorge Malheiro, Himmet Bora Uslu, Alun Williams, Vincenzo Cantaluppi, Yunying Shi, La Salete Martins, Pål-Dag Line, Péter Szakály, and Takaaki Kobayashi

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, Medicine, business, Surgery
Published
2012
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4. Multiple myeloma: reduced plasma cell contamination in peripheral blood progenitor cell collections performed after repeated high-dose chemotherapy courses

Author

Roberta Triolo, Roberta Ravaglia, S Triolo, Antonio Palumbo, Alida Dominietto, Alessandro Pileri, Fulvia Giaretta, Paolo Corradini, Paola Omedè, C Argentino, Daniele Caracciolo, Corrado Tarella, and Mario Boccadoro

Subjects
Adult, Male, Vincristine, medicine.medical_specialty, Pathology, Cyclophosphamide, medicine.medical_treatment, Plasma Cells, Urology, Antigens, CD34, Pilot Projects, Plasma cell, Antineoplastic Combined Chemotherapy Protocols, Humans, Medicine, Leukapheresis, Progenitor cell, Multiple myeloma, Etoposide, Dexamethasone, Aged, Chemotherapy, business.industry, Hematology, Middle Aged, Prognosis, medicine.disease, Hematopoietic Stem Cell Mobilization, medicine.anatomical_structure, Female, Multiple Myeloma, business, medicine.drug
Abstract

The possibility of reducing tumour cell contamination by cytotoxic drug courses prior to peripheral blood progenitor cell (PBPC) collection was evaluated in two consecutives groups of multiple myeloma (MM) patient candidates for autograft. All patients were at disease onset and received two VAD (vincristine. doxorubicin and dexamethasone) courses as initial debulking. In the first group (44 patients), mobilization and harvest were performed 'upfront'. after a single cyclophosphamide (CY) administration of 4 g/m 2 : in the second group (17 patients). PBPC were collected at the end of a high-dose sequential chemotherapy programme, including: CY 5 g/m 2 , etoposide (VP16) 2 g/m 2 . a chemotherapy-free interval with three courses of high-dose dexamethasone, a final mobilizing CY at 7 g/m 2 . G-CSF was given following each high-dose cytotoxic drug. Cytofluorimetric analysis was performed to quantify progenitors (CD34 + cells) and plasma cells, identified by the high CD38 expression and/or CD38 and CD138 coexpression. Large amounts of PBPC were collected in either group (median harvested CD34 + /kg: 15.8 x10 6 and 13.4 x 10 6 , respectively; P= 0.9). Circulating plasma cells were significantly higher in patients mobilized 'upfront' compared to those who received the high-dose sequence (median peak values of CD38 bright /μl: 39 and 10, respectively; P= 0.02); a similar difference was observed in the amount of contaminating plasma cells in the harvest products (median CD38 bright /kg: 7.4 x 10 6 and 1.3x10 6 , respectively; P= 0.02). The results demonstrate that an in vivo purging approach is feasible in myeloma patients through repeated high-dose chemotherapy courses; this may provide less-contaminated material suitable for further in vitro purging procedures.

Published
1997
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5. Peripheral blood progenitor cell mobilization in patients with primary refractory lymphoma or at first relapse: comparison with patients at diagnosis and impact on clinical outcome

Author

Paolo Gavarotti, Daniele Caracciolo, Alessandro Pileri, Fulvia Giaretta, Paolo Corradini, Claudia Castellino, P Bondesan, Corrado Tarella, and C Cherasco

Subjects
Adult, Male, medicine.medical_specialty, Cyclophosphamide, medicine.medical_treatment, Filgrastim, Gastroenterology, Disease-Free Survival, Refractory, Recurrence, Internal medicine, medicine, Humans, Child, Antineoplastic Agents, Alkylating, Etoposide, Chemotherapy, business.industry, Lymphoma, Non-Hodgkin, Hematopoietic Stem Cell Transplantation, Hematology, Leukapheresis, Middle Aged, medicine.disease, Antineoplastic Agents, Phytogenic, Hodgkin Disease, Hematopoietic Stem Cell Mobilization, Lymphoma, Granulocyte colony-stimulating factor, Surgery, Treatment Outcome, Female, business, medicine.drug
Abstract

Peripheral blood progenitor cell (PBPC) mobilization was evaluated in 53 patients receiving the high-dose sequential (HDS) regimen: 27 had non-Hodgkin's lymphoma or Hodgkin's disease, primary refractory or at first relapse, 26 had non-Hodgkin's lymphoma at diagnosis. Mobilization was assessed following either 7 g/m2 cyclophosphamide (48 patients) or 2 g/m2 etoposide, both followed by G-CSF (filgrastim) at 5 microg/kg/d. PBPC mobilization was significantly higher in patients at diagnosis compared to refractory/relapsed patients (median peak values of circulating CFU-GM: 25,209/ml v 4270/ml, P < 0.0001 and CD34+ cells: 286/microl v 47/microl, P < 0.0001). All patients receiving HDS as up-front treatment mobilized enough PBPC for an autograft, often requiring a single leukapheresis; whereas only 15 patients under salvage treatment with HDS were able to complete PBPC autograft. Bone marrow (BM) cells, alone or with PBPC, were needed in six patients, and autograft could not be performed in six patients. Among refractory/relapsed patients, those having a high PBPC mobilization experienced a significantly longer EFS compared to those who had not; autograft completion also significantly enhanced EFS. Thus, the use of an effective mobilizing protocol does not ensure adequate PBPC mobilization in moderately pretreated patients; low mobilization must be considered as an early sign of poor outcome in patients receiving a high-dose salvage programme.

Published
1997
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6. Real-time polymerase chain reaction in multiple myeloma: quantitative analysis of tumor contamination of stem cell harvests

Author

Marco, Ladetto, Paola, Omedè, Selina, Sametti, John W, Donovan, Monica, Astolfi, Daniela, Drandi, Federica, Volpato, Luisa, Giaccone, Fulvia, Giaretta, Antonio, Palumbo, Benedetto, Bruno, Alessandro, Pileri, John G, Gribben, and Mario, Boccadoro

Subjects
Adult, Gene Rearrangement, Hematopoietic Stem Cell Transplantation, Reproducibility of Results, Bone Marrow Cells, Middle Aged, Flow Cytometry, Hematopoietic Stem Cells, Polymerase Chain Reaction, Hematopoietic Stem Cell Mobilization, Humans, Immunoglobulin Heavy Chains, Multiple Myeloma, Cyclophosphamide, Immunosuppressive Agents
Abstract

Autologous transplantation of bone marrow (BM) and peripheral blood progenitor cells (PBPC) is commonly used for treatment of multiple myeloma (MM). Although both stem cell sources harbor residual clonal cells, a quantitative evaluation of their level of tumor contamination (LTC) still needs to be performed through highly accurate and reproducible approaches. In this study, we used a validated real-time polymerase chain reaction (PCR) strategy to evaluate LTC of BM and PBPC samples obtained from MM patients.The patients underwent two different mobilization courses (defined as early or late course) following two cycles of cyclophosphamide 5 g/m(2). LTC was evaluated by measuring the number of clonal immunoglobulin heavy-chain rearrangements followed by normalization of samples using the GAPDH gene.Overall, 26 PBPC and 12 BM samples were analyzed. Main results are as follows. 1) PBPC harvests are less contaminated than BM samples taken immediately after each mobilization course (median difference 2.68 logs; range 1.7 to 4.6) (p0.0001). 2) LTC of PBPC harvests has only minimal variation among different leukaphereses performed during the same mobilization course (median difference 0.45 logs; range 0.22 to 1.2). 3) No difference was observed among PBPC and BM samples obtained after the late mobilization course as compared to the early mobilization course (median reduction 0.21 logs; range -0.39 to 1.3) (p = 0.84). 4) In PBPC but not in BM samples, there is a clear overestimation of the percentage of plasma cells when flow cytometric evaluation of CD38(bright) cells is compared to real-time PCR results. This suggests that in PBPC, most CD38(bright) cells do not belong to the neoplastic clone.Real-time PCR using the IgH rearrangement proved an effective tool for monitoring LTC in stem cell harvests from MM patients. The smaller LTC of PBPC harvests supports the role of PBPC as stem cell rescue for MM patients compared to BM cells.

Published
2002

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