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Glulam timber is gaining popularity as a structural element due to its cost-effectiveness, strength, and environmental benefits. However, being an organic material, it is susceptible to degradation. Therefore, nondestructive testing (NDT) methods are essential for assessing its structural health. In this study, two NDT approaches, ultrasound-based and modal updating, were used to predict the static bending modulus of elasticity (MOE) in Pinus Sylvestris and Spruce species. The results demonstrated that the modal updating and linear regression models outperformed the ultrasound technique in accurately determining the static MOE. Additionally, considering the influence of knots improved the accuracy of ultrasound predictions. [ABSTRACT FROM AUTHOR]