Silva, Nickolas Menezes da, Reis, Guilherme Fonseca, Costa, Flávia de Fátima, Grisolia, Maria Eduarda, Geraldo, Marlon Roger, Lustosa, Bruno Paulo Rodrigues, Lima, Bruna Jacomel Favoreto de Souza, Weiss, Vinicius Almir, de Souza, Emanuel Maltempi, Li, Ruoyu, Song, Yinggai, Nascimento, Mariana Machado Fidelis, Robl, Diogo, Gomes, Renata Rodrigues, de Hoog, G. Sybren, and Vicente, Vania Aparecida
Cladophialophora exuberans is a filamentous fungus related to black yeasts in the order Chaetothyriales. These melanized fungi are known for their 'dual ecology', often occurring in toxic environments and also being frequently involved in human infection. Particularly Cladophialophora exuberans, C. immunda , C. psammophila, and Exophiala mesophila have been described with a pronounced ability to degrade aromatic compounds and xenobiotic volatiles, such as benzene, toluene, ethyl-benzene, and xylene, and are candidates for bioremediation applications. The objective of the present study is the sequencing, assembly, and description of the whole genome of C. exuberans focusing on genes and pathways related to carbon and toxin management, assessing the tolerance and bioremediation of lead and copper, and verifying the presence of genes for metal homeostasis. Genomic evaluations were carried out through a comparison with sibling species including clinical and environmental strains. Tolerance of metals was evaluated via a microdilution method establishing minimum inhibitory (MIC) and fungicidal concentrations (MFC), and agar diffusion assays. Heavy metal bioremediation was evaluated via graphite furnace atomic absorption spectroscopy (GFAAS). The final assembly of C. exuberans comprised 661 contigs, with genome size of 38.10 Mb, coverage of 89.9X and a GC content of 50.8%. In addition, inhibition of growth was shown at concentrations of 1250 ppm for copper and at 625 ppm for lead, using the MIC method. In the agar tests, the strain grew at 2500 ppm of copper and lead. In GFAAS tests, uptake capacities were observed of 89.2% and 95.7% for copper and lead, respectively, after 21 experimental days. This study enabled the annotation of genes involved in heavy metal homeostasis and also contributed to a better understanding of the mechanisms used in tolerance of and adaptation to extreme conditions. • Cladophialophora exuberans genome analysis revealed 14 genes to metal assimilation. • Bioremediation novel candidate of copper and lead with high rates of tolerance. • Possible genetic pathways involved in tolerance/adaptation to extreme conditions. [ABSTRACT FROM AUTHOR]