34 results on '"Hameed, Huma"'
Search Results
2. Maximizing the extraction yield of plant gum exudate using response surface methodology and artificial neural networking and pharmacological characterization
- Author
-
Noureen, Shazia, Noreen, Sobia, Ghumman, Shazia Akram, Al-Hussain, Sami A., Hameed, Huma, Anwar-Ul-Haq, Muhammad, Irfan, Ali, Batool, Fozia, Hassan, Muhammad Umair, Aslam, Samina, and Zaki, Magdi E. A.
- Published
- 2023
- Full Text
- View/download PDF
3. Chitin: A versatile biopolymer-based functional therapy for cartilage regeneration
- Author
-
Hameed, Huma, Khan, Mahtab Ahmad, Paiva-Santos, Ana Cláudia, Ereej, Nelofer, and Faheem, Saleha
- Published
- 2024
- Full Text
- View/download PDF
4. Anti-aging peptides for advanced skincare: Focus on nanodelivery systems
- Author
-
Veiga, Eliana, Ferreira, Laura, Correia, Mafalda, Pires, Patrícia C., Hameed, Huma, Araújo, André R.T. S., Cefali, Letícia Caramori, Mazzola, Priscila Gava, Hamishehkar, Hamed, Veiga, Francisco, and Paiva-Santos, Ana Cláudia
- Published
- 2023
- Full Text
- View/download PDF
5. Lauric acid provides neuroprotection against oxidative stress in mouse model of hyperglycaemic stroke
- Author
-
Shaheryar, Zaib Ali, Khan, Mahtab Ahmad, Hameed, Huma, Zaidi, Syed Awais Ali, Anjum, Irfan, and Rahman, Muhammad Shafeeq Ur
- Published
- 2023
- Full Text
- View/download PDF
6. Mimosa pudica mucilage nanoparticles of losartan potassium: Characterization and pharmacodynamics evaluation
- Author
-
Ghumman, Shazia Akram, Mahmood, Arshad, Noreen, Sobia, Hameed, Huma, Kausar, Rizwana, Rana, Maria, and Aslam, Asma
- Published
- 2023
- Full Text
- View/download PDF
7. Chitosan-Linseed mucilage polyelectrolyte complex nanoparticles of Methotrexate: In vitro cytotoxic efficacy and toxicological studies
- Author
-
Akram Ghumman, Shazia, Mahmood, Arshad, Noreen, Sobia, Aslam, Asma, Ijaz, Bushra, Amanat, Amina, Kausar, Rizwana, Rana, Mavra, and Hameed, Huma
- Published
- 2023
- Full Text
- View/download PDF
8. Formulation and evaluation of quince seeds mucilage – sodium alginate microspheres for sustained delivery of cefixime and its toxicological studies
- Author
-
Ghumman, Shazia Akram, Mahmood, Arshad, Noreen, Sobia, Rana, Mavra, Hameed, Huma, Ijaz, Bushra, Hasan, Sara, Aslam, Afeefa, and ur Rehman, Muhammad Fayyaz
- Published
- 2022
- Full Text
- View/download PDF
9. Exploring the potential of nanomedicine for gene therapy across the physicochemical and cellular barriers.
- Author
-
Hameed, Huma, Sarwar, Hafiz Shoaib, Younas, Komel, Zaman, Muhammad, Jamshaid, Muhammad, Irfan, Ali, Khalid, Maha, and Sohail, Muhammad Farhan
- Abstract
After COVID-19, a turning point in the way of pharmaceutical technology is gene therapy with beneficial potential to start a new medical era. However, commercialization of such pharmaceuticals would never be possible without the help of nanotechnology. Nanomedicine can fulfill the growing needs linked to safety, efficiency, and site-specific targeted delivery of Gene therapy-based pharmaceuticals. This review's goal is to investigate how nanomedicine may be used to transfer nucleic acids by getting beyond cellular and physicochemical barriers. Firstly, we provide a full description of types of gene therapy, their mechanism, translation, transcription, expression, type, and details of diseases with possible mechanisms that can only be treated with genes-based pharmaceuticals. Additionally, we also reviewed different types of physicochemical barriers, physiological and cellular barriers in nucleic acids (DNA/RNA) based drug delivery. Finally, we highlight the need and importance of cationic lipid-based nanomedicine/nanocarriers in gene-linked drug delivery and how nanotechnology can help to overcome the above-discussed barrier in gene therapy and their biomedical applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. A comprehensive review on lipid-based nanoparticles via nose to brain targeting as a novel approach.
- Author
-
Hameed, Huma, Faheem, Saleha, Younas, Komel, Jamshaid, Muhammad, Ereej, Nelofer, Hameed, Anam, Munir, Rabia, and Khokhar, Rabia
- Subjects
- *
CENTRAL nervous system , *DRUG carriers , *LIPOSOMES , *NANOPARTICLES , *NANOTECHNOLOGY , *BLOOD-brain barrier , *MUCOCILIARY system - Abstract
AbstractThe central nervous system (CNS) has been a chief concern for millions of people worldwide, and many therapeutic medications are unable to penetrate the blood-brain barrier. Advancements in nanotechnology have enabled safe, effective, and precise delivery of medications towards specific brain regions by utilising a nose-to-brain targeting route. This method reduces adverse effects, increases medication bioavailability, and facilitates mucociliary clearance while promoting accumulation of drug in the targeted brain region. Recent developments in lipid-based nanoparticles, for instance solid lipid nanoparticles (SLNs), liposomes, nanoemulsions, and nano-structured lipid carriers have been explored. SLNs are currently the most promising drug carrier system because of their capability of transporting drugs across the blood-brain barrier at the intended brain site. This approach offers higher efficacy, controlled drug delivery, target specificity, longer circulation time, and a reduction in toxicity through a biomimetic mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
11. DMARDS incorporated injectable hydrogels in cartilage tissue regeneration: a review.
- Author
-
Ayaz, Urwa, Zaman, Muhammad, Hameed, Huma, Naeem, Saba, Noor Khan, Iqra, Ahsan Waqar, Muhammad, Niaz, Sania, Ereej, Nelofer, and Salawi, Ahmad
- Abstract
In the field of regenerative medicine, cartilage tissue regeneration presents a challenge because it is not sufficiently self-healing. Rheumatic diseases like osteoarthritis and rheumatoid arthritis, among various others, contribute to cartilage damage causing pain, inflammation and immobility of joints. In recent years, administration of disease modifying anti-rheumatic drugs (DMARDs) by incorporating them into injectable hydrogels has emerged as a promising approach to cartilage tissue regeneration. DMARDs exhibit anti-inflammatory and immunomodulatory properties which contribute in reduction of inflammation. These characteristics enable them to control inflammatory response, encourage chondrogenesis and thereby facilitate regeneration of cartilage. Pre-clinical and clinical research has been reported for safety and effectiveness where hydrogels containing DMARDs show promising efficacy and safety. Drug concentrations of DMARDs are tailored based on effective concentration, delivery, structural integrity of hydrogels in consideration with pharmacodynamics and physicochemical properties. Ongoing research is evaluating long-term safety and therapeutic potential. Some hydrogel systems are already approved and commercially available, while others are in optimization studies. This review summarizes current landscape of injectable hydrogels containing DMARDs, highlighting their application in cartilage tissue regeneration, encompassing both pre-clinical and clinical research that has evidenced safety and effectiveness and also providing insights into market prospects. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
12. Liposomes like advanced drug carriers: from fundamentals to pharmaceutical applications.
- Author
-
Hameed, Huma, Khan, Mahtab Ahmad, Paiva-Santos, Ana Cláudia, Faheem, Saleha, Khalid, Aleena, Majid, Muhammad Sohaib, Adnan, Aiman, and Rana, Fizza
- Subjects
- *
DRUG delivery systems industry , *LIPOSOMES , *DRUG carriers , *RESEARCH personnel , *ELECTRONIC information resource searching - Abstract
There are around 24 distinct lipid vesicles described in the literature that are similar to vesicular systems such as liposomes. Liposome-like structures are formed by combining certain amphiphilic lipids with a suitable stabiliser. Since their discovery and classification, self-assembled liposome-like structures as active drug delivery vehicles captured researchers' curiosity. This comprehensive study included an in-depth literature search using electronic databases such as PubMed, ScienceDirect and Google Scholar, focusing on studies on liposome and liposomes like structure, discussed in literature till 2024, their sizes, benefits, drawback, method of preparation, characterisation and pharmaceutical applications. Pharmacosomes, cubosomes, ethosomes, transethosomes, and genosomes, all liposome-like structures, have the most potential due to their smaller size with high loading capacity, ease of absorption, and ability to treat inflammatory illnesses. Genosomes are futuristic because of its affinity for DNA/gene transport, which is an area of focus in today's treatments. This review will critically analyse the composition, preparation procedures, drug encapsulating technologies, drug loading, release mechanism, and related applications of all liposome-like structures, highlighting their potential benefits with enhanced efficacy over each other and over traditional carriers by paving the way for exploring novel drug delivery systems in the Pharma industry. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
13. Ethosomes: a potential nanovesicular carrier to enhancing the drug delivery against skin barriers.
- Author
-
Hameed, Huma, Faheem, Saleha, Khan, Mahtab Ahmad, Hameed, Anam, Ereej, Nelofer, and Ihsan, Hafsa
- Subjects
- *
SKIN permeability , *DRUG carriers , *TOPICAL drug administration , *DRUG bioavailability , *DRUG efficacy , *SMALL molecules , *FILAGGRIN - Abstract
Ethosomes, which are liposomes like structures, mainly composed primarily of ethanol, have attracted considerable attention due to their potential to enhance the drug permeation via skin. The article discusses the formulation and preparation methods of ethosomes, offering insights into the various factors that influence their size, shape, and stability. Moreover, it explores the techniques used to assess the physicochemical properties of ethosomes and their impact on drug delivery effectiveness. The article also elucidates the mechanism by which ethosomes enhance skin permeation, emphasising their ability to modify the lipid structure and fluidity of the stratum corneum. Additionally, the review investigates the applications of ethosomes in diverse drug delivery scenarios, including the delivery of small molecules, peptides, and phytoconstituents. It highlights the potential of ethosomes to improve drug bioavailability, extend drug release, and achieve targeted delivery to specific skin layers or underlying tissues. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
14. Evaluation of Plant-Based Silver Nanoparticles for Antioxidant Activity and Promising Wound-Healing Applications.
- Author
-
Qubtia, Maria, Ghumman, Shazia Akram, Noreen, Sobia, Hameed, Huma, Noureen, Shazia, Kausar, Rizwana, Irfan, Ali, Akhtar Shah, Pervaiz, Afzal, Hafsa, Hameed, Misbah, Raish, Mohammad, Rana, Maria, Ahmad, Ajaz, Kotwica-Mojzych, Katarzyna, and Bin Jardan, Yousef A.
- Published
- 2024
- Full Text
- View/download PDF
15. Preparation and Evaluation of a Self-Emulsifying Drug Delivery System for Improving the Solubility and Permeability of Ticagrelor.
- Author
-
Aziz, Anam, Zaman, Muhammad, Khan, Mahtab Ahmad, Jamshaid, Talha, Butt, Muhammad Hammad, Hameed, Huma, Rahman, Muhammad Shafeeq Ur, and Shoaib, Qurat-ul-Ain
- Published
- 2024
- Full Text
- View/download PDF
16. A comprehensive review on transethosomes as a novel vesicular approach for drug delivery through transdermal route.
- Author
-
Munir, Minahal, Zaman, Muhammad, Waqar, Muhammad Ahsan, Hameed, Huma, and Riaz, Tehseen
- Subjects
TRANSDERMAL medication ,PARTICLE size determination ,PATIENT compliance ,ZETA potential ,TRANSITION temperature ,ANALGESICS - Abstract
Drug delivery through transdermal route is one of the effective methods for the application of drugs. It overcomes many drawbacks which are encountered with the oral route. Moreover, many drugs are not able to pass through the stratum corneum, which is the main barrier for the transdermal drug delivery. Formation of ultra-deformable vesicles (UDVs) is a novel technique for the transdermal applications of the drugs. Transethosomes (TEs), ethosomes, and transferosomes are all part of the UDV. Because of the presence of increased concentrations of ethanol, phospholipids, and edge activators, TEs provide improved drug permeation through the stratum corneum. Because of the elasticity of TEs, drug penetration into the deeper layer of skin also increases. TEs can be prepared using a variety of techniques, including the cold method, hot method, thin film hydration method, and the ethanol injection method. It increases patient adherence and compliance because it is a non-invasive procedure of administering drugs. Characterization of the TEs includes pH determination, size and shape, zeta potential, particle size determination, transition temperature, drug content, vesicle stability, and skin permeation studies. These vesicular systems can be utilized to deliver a variety of medications transdermally, including analgesics, antibiotics, antivirals, and anticancer and arthritis medications. This review aims to describe vesicular approaches that had been used to overcome the barrier for the transdermal delivery of drug and also describes brief composition, method of preparation, characterization tests, mechanism of penetration of TEs, as well as highlighted various applications of TEs in medicine. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute Hyperglycemia.
- Author
-
Shaheryar, Zaib Ali, Khan, Mahtab Ahmad, Hameed, Huma, Mushtaq, Muhammad Naveed, Muhammad, Sajjad, Shazly, Gamal A., Irfan, Ali, and Jardan, Yousef A. Bin
- Subjects
ENDOTHELIAL cells ,CELL death ,SATURATED fatty acids ,HYPERGLYCEMIA ,FATTY acids - Abstract
Ischemic stroke is worsened by the presence of sudden high blood sugar levels, even in individuals without pre-existing diabetes. This elevated glucose concentration hampers the ability of energy-starved brain cells to efficiently use it as a source of energy. Consequently, this leads to the production of abundant amounts of toxic glucose metabolites, which trigger oxidative stress in the brain milieu, particularly in the microvasculature of the brain. A prominent feature of this oxidative stress is the demise of endothelial cells, causing detrimental changes in blood vessels, including a reduction in their vascular diameter, a decreased efficiency of vessel proliferation, and the impaired integrity of tight junctions. These vascular pathologies contributed to an increase in the volume of damaged tissues (infarct), an exacerbation of brain swelling (edema), and a decline in cognitive and motor functions. In a mouse model of ischemic stroke with induced acute hyperglycemia, a naturally occurring saturated fatty acid provides protective cover to the microvasculature by preventing damage related to oxidative stress. Our current research revealed that lauric acid (LA) attenuated infarct volume and reduced brain edema by reducing endothelial cell death, enhancing vessels' diameter, promoting vascular angiogenesis, and stabilizing barrier functions. Animals administered with this natural compound showed a significant reduction in 4-HNE-positive vessels. In conclusion, natural saturated fatty acids help to preserve brain microvascular functions following ischemic insults in the presence of acute hyperglycemia. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
18. Prevalence of Eating Disorders and Their Association with Social Media Addiction among Youths.
- Author
-
Mushtaq, Tehreem, Ashraf, Seemab, Hameed, Huma, Irfan, Ali, Shahid, Maria, Kanwal, Rabbia, Aslam, Muhammad Arslan, Shahid, Hijab, Koh-E-Noor, Shazly, Gamal A., Khan, Mahtab Ahmad, and Jardan, Yousef A. Bin
- Abstract
Eating disorders and excessive attachment to social media are a matter of great concern among youths. This study assessed the prevalence of eating disorders and their association with social media addiction among youths. A descriptive cross-sectional study was conducted on 350 participants aged 14–25 years. Two pre-validated tools were used, i.e., the Eating Attitude Test and the Social Networking Addiction Scale. SPSS was used to analyze the data. Out of the 350 students, 42% had probable eating disorders, and 41.7% had social media addictions. The findings revealed that the chances of having eating disorders were significantly higher among youths who lived in separate places, smoked, and had a family history of eating disorders (p ≤ 0.05). Furthermore, the dieting domain displayed notably higher scores for youths living separately (p ≤ 0.05) and smokers (p ≤ 0.01). Moreover, the scores for bulimia and food preoccupation were significantly higher among participants who were married (p = 0.038), were smokers (p = 0.027), and had a family history of eating disorders (p = 0.001). Higher scores in the oral control domain were reported by females (p ≤ 0.05) and severely obese youths (p ≤ 0.01). Moreover, social media addiction was significantly higher among students aged 18–21 (p ≤ 0.01). Spearman's correlation revealed that social media addiction has a weak positive relationship with eating disorders (r = 0.133, p ≤ 0.01), particularly bulimia and food preoccupation (r = 0.173, p ≤ 0.001). This reflects the need to address the harmful consequences of social media addiction that might raise the likelihood of developing eating disorders, particularly bulimia nervosa. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
19. Formulation, Characterization, and Evaluation of β‑Cyclodextrin Functionalized Hypericin Loaded Nanocarriers.
- Author
-
Waqar, Muhammad Ahsan, Zaman, Muhammad, Hameed, Huma, Jamshaid, Muhammad, Irfan, Ali, Shazly, Gamal A., Paiva-Santos, Ana Cláudia, and Bin Jardan, Yousef A.
- Published
- 2023
- Full Text
- View/download PDF
20. Thermo-Responsive Sol-Gel-Based Nano-Carriers Containing Terbinafine HCl: Formulation, In Vitro and Ex Vivo Characterization, and Antifungal Activity.
- Author
-
Bajwa, Maryam, Tabassam, Naila, Hameed, Huma, Irfan, Ali, Zaman, Muhammad, Khan, Mahtab Ahmad, Shazly, Gamal A., Mehboob, Tooba, Riaz, Tehseen, and Jardan, Yousef A. Bin
- Subjects
SOL-gel materials ,TERBINAFINE ,POLOXAMERS ,DISTILLED water ,X-ray diffraction - Abstract
The current research aims to create a sol-gel-based nanocarrier containing terbinafine formulated for transdermal delivery of the drug into the skin. Sol-gel-based nanocarriers were prepared via the cold method using poloxamer-188, poloxamer-407, and distilled water. The prepared formulation was examined for pH, gelation temperature, Fourier transform infrared spectrophotometer (FTIR) analysis, thermal stability analysis, X-ray diffraction (XRD), scanning electron microscopy (SEM), particle size analysis, zeta potential, and anti-microbial activity. The in-vitro drug release study of F1 was found to be 94%, which showed greater drug release as compared to F2 and F3. The pH of the formulation was found to be within the range applicable to the skin. The gelation temperature was detected at 28 °C. The SEM images of formulations have spotted various particles well-segregated from each other. Analysis of formulations showed a mean globule size diameter of 428 nm, zeta potential values of 0.04 mV, refractive index (1.329), and viscosity (5.94 cP). FTIR analysis confirmed various functional groups' presence in the prepared formulation. Thermal analysis has confirmed the stability of the drug within the prepared formulation. The growth of inhibition was found to be 79.2% in 60 min, which revealed that the prepared formulation has shown good permeation from the membrane. Hence, the sol-gel-based nanocarrier formulation of terbinafine was successfully developed and evaluated. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
21. Lamotrigine-Loaded Poloxamer-Based Thermo-Responsive Sol–Gel: Formulation, In Vitro Assessment, Ex Vivo Permeation, and Toxicology Study.
- Author
-
Riaz, Maria, Zaman, Muhammad, Hameed, Huma, Sarwar, Hafiz Shoaib, Khan, Mahtab Ahmad, Irfan, Ali, Shazly, Gamal A., Paiva-Santos, Ana Cláudia, and Jardan, Yousef A. Bin
- Subjects
LAMOTRIGINE ,POLOXAMERS ,SOL-gel processes ,TOXICOLOGY ,GELATION - Abstract
The present study aimed to prepare, characterize, and evaluate a thermo-responsive sol–gel for intranasal delivery of lamotrigine (LTG), which was designed for sustained drug delivery to treat epilepsy. LTG sol–gel was prepared using the cold method by changing the concentrations of poloxamer 407 and poloxamer 188, which were used as thermo-reversible polymers. The optimized formulations of sol–gel were analyzed for clarity, pH, viscosity, gelation temperature, gelation time, spreadability, drug content, in vitro drug release studies, ex vivo permeation studies, and in vivo toxicological studies. FTIR, XRD, and DSC were performed to determine the thermal stability of the drug and polymers. The prepared formulations had a clear appearance in sol form; they were liquid at room temperature and became gel at temperatures between 31 °C and 36 °C. The pH was within the range of the nasal pH, between 6.2 and 6.4. The drug content was found to be between 92% and 94%. In vitro drug release studies indicated that the formulations released up to 92% of the drug within 24 h. The FTIR, DSC, and XRD analyses showed no interaction between the drug and the polymer. A short-term stability study indicated that the formulation was stable at room temperature and at 4–8 °C. There was a slight increase in viscosity at room temperature, which may be due to the evaporation of the vehicle. A histological study indicated that there were no signs of toxicity seen in vital organs, such as the brain, kidney, liver, heart, and spleen. It can be concluded from the above results that the prepared intranasal sol–gel for the delivery of LTG is safe for direct nose-to-brain delivery to overcome the first-pass effect and thus enhance bioavailability. It can be considered an effective alternative to conventional drug delivery for the treatment of epilepsy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
22. Anti-Arthritic and Immunomodulatory Potential of Methanolic, n-Hexane, and Ethyl Acetate Fractions of Bark of Acacia modesta on Complete Freund's Adjuvant-Induced Arthritis in Rats.
- Author
-
Mashaal, Kiran, Shabbir, Arham, Khan, Mahtab Ahmad, Hameed, Huma, Shahzad, Muhammad, Irfan, Ali, Shazly, Gamal A., Mobashar, Aisha, Akhtar, Tasleem, Shaheryar, Zaib Ali, and Bin Jardan, Yousef A.
- Subjects
ADJUVANT arthritis ,ETHYL acetate ,HEXANE ,ACACIA ,THERAPEUTICS ,RHEUMATOID arthritis ,AUTOIMMUNE diseases ,BLOOD cell count - Abstract
Rheumatoid arthritis is an autoimmune disorder and topic of interest for researchers due to its increasing frequency and limited treatment. Acacia modesta Wall is known to treat rheumatic disorders in the traditional system of medicinal plants. Traditional medicines are still required for the treatment of this disease due to the large number of side-effects caused by commercial medicines. In the current study, the antiarthritic potential of methanolic extract (AM-metha), n-hexane (AM-hexa) fraction, and ethyl acetate (AM-etha) fraction of the bark of A. modesta against a complete Freund's adjuvant rat model was evaluated. Evaluation using a digital plethysmometer, macroscopic evaluation, and histopathological evaluation were conducted to determine the paw volume and arthritic scoring. ELISA was performed to assess the PGE2 levels. RT-PCR was used to evaluate the expression levels of MMP2, MMP3, MMP9, NF-κB, IL6, IL1β, TNFα, and VEGF. Biochemical and hematological analyses were also conducted. GC/MS was also carried out to analyze the presence of medicinal compounds. The data revealed a marked reduction in the paw volume, arthritic scoring, and histopathological parameters, indicating the anti-arthritic potential of the plant. Treatment with plant extracts and fractions markedly down-regulated MMP2, MMP3, MMP9, NF-κB, IL6, IL1β, TNFα, and VEGF levels. Similarly, PGE2 levels were also found to be ameliorated in the treatment groups, indicating the immunomodulatory property of plant bark. Plant treatment nearly normalized hematological parameters such as counts of WBCs, RBCs, and platelets, along with Hb content, thereby validating the anti-arthritic activity. GC/MS analysis disclosed the presence of strong anti-inflammatory compounds such as lupeol, oleic acid, and squalene. The study showed that A. modesta possesses anti-arthritic and immunomodulatory potential linked to significant down-regulation of pro-inflammatory and inflammatory biomarkers. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
23. Design and Evaluation of Hydrophobic Ion Paired Insulin Loaded Self Micro-Emulsifying Drug Delivery System for Oral Delivery.
- Author
-
Mudassir, Jahanzeb, Raza, Afsheen, Khan, Mahtab Ahmad, Hameed, Huma, Shazly, Gamal A., Irfan, Ali, Rana, Sadia Jafar, Abbas, Khizar, Arshad, Muhammad Sohail, Muhammad, Sajjad, and Bin Jardan, Yousef A.
- Subjects
DRUG delivery systems ,ION pairs ,INSULIN ,PROTEOLYSIS ,FACTORIAL experiment designs ,SODIUM sulfate - Abstract
Despite several novel and innovative approaches, clinical translation of oral insulin delivery into commercially viable treatment is still challenging due to its poor absorption and rapid degradation in GIT. Thus, an insulin-SDS hydrophobic ion pair loaded self-microemulsifying drug delivery system (SMEDDS) was formulated to exploit the hypoglycemic effects of orally delivered insulin. Insulin was initially hydrophobically ion paired with sodium dodecyl sulphate (SDS) to enhance its lipophilicity. The successful complexation of Insulin-SDS was confirmed by FTIR and surface morphology was evaluated using SEM. Stability of insulin after its release from HIP complex was evaluated using SDS PAGE. Subsequently, Ins-SDS loaded SMEDDS was optimized using two factorial designs. In vitro stability of insulin entrapped in optimized SMEDDS against proteolytic degradation was also assessed. Further, antidiabetic activity of optimized Ins-SDS loaded SMEDDS was evaluated in diabetic rats. Insulin complexed with SDS at 6:1 (SDS/insulin) molar ratio with almost five-fold increased lipophilicity. The SMEDDS was optimized at 10% Labraphil M2125 CS, 70% Cremophore EL, and 20% Transcutol HP with better proteolytic stability and oral antidiabetic activity. An Ins-SDS loaded SMEDDS was successfully optimized. Compared with insulin and Ins-SDS complex, the optimized SMEDDS displayed considerable resistance to GI enzymes. Thus, the SMEDDS showed potential for effective delivery of macromolecular drugs with improved oral bioavailability. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
24. A Review of Past 32 Years of Human Development Reports: Conceptualization Challenge and Way Forward.
- Author
-
Rasool, Hassan and Hameed, Huma
- Subjects
HUMAN Development Index ,HAMILTON Depression Inventory ,HUMAN beings - Abstract
This paper reviews the Human Development Reports (HDRs) of the past 32 years to evaluate changes in the conceptualization of the Human Development Index (HDI) in determining human development over time. Using a genealogical approach, the data is extracted from HDRs that were published during 1990-2022. We identify that no significant change occurred in the conceptualization of human development over a period of more than three decades. It was also identified that the changing needs of human development were not addressed holistically in the measurement of human development overtime. The changes that occurred over time were either event-driven or remained tentative to include more important attributes of human development. Furthermore, the human development concept as identified in HDRs and measured through HDI is neither grounded in theory nor takes into account the interdisciplinary nature of human development. We discuss how interdisciplinary knowledge could be used to overcome challenges in the better conceptualization of human development for preparing a more representative human development index (HDI). [ABSTRACT FROM AUTHOR]
- Published
- 2023
25. In Vitro/In Vivo Evaluation of Clomipramine Orodispersible Tablets for the Treatment of Depression and Obsessive-Compulsive Disorder.
- Author
-
Ghumman, Shazia Akram, Hameed, Huma, Noreen, Sobia, Al-Hussain, Sami A., Kausar, Rizwana, Irfan, Ali, Shabbir, Ramla, Rana, Maria, Amanat, Amina, and Zaki, Magdi E. A.
- Subjects
- *
OBSESSIVE-compulsive disorder , *MUCILAGE , *FACTORIAL experiment designs , *MENTAL depression , *SEROTONIN , *DEEP brain stimulation , *DRUG marketing - Abstract
The first and only antidepressant drug on the market with solid proof of clinically significant serotonin and noradrenaline reuptake inhibition is clomipramine (CLP). However, significant first-pass metabolism reduces its absorption to less than 62%. It is heavily protein-bound and broadly dispersed across the body (9–25 L/kg volume of distribution). The purpose of this research was to formulate CLP orodispersible tablets that immediately enable the drug to enter the bloodstream and bypass systemic portal circulation to improve its bioavailability. A factorial design was employed using varied amounts of Plantago ovata mucilage (POM) as a natural superdisintegrant, as well as croscarmellose sodium and crospovidone as synthetic disintegrants. Their physiochemical compatibility was evaluated by FTIR, DSC/TGA, and PXRD analysis. The blend of all formulations was assessed for pre- and post-compaction parameters. The study found that tablets comprising Plantago ovata mucilage as a superdisintegrant showed a rapid in vitro disintegration time, i.e., around 8.39 s, and had an excellent dissolution profile. The anti-depressant efficacy was evaluated by an open-field test (OFT) and the forced swimming test (FST) was applied to create hopelessness and despair behavior as a model of depression in animals (Albino rats). The in vivo study revealed that the efficiency of the optimized formulation (F9) in the treatment of depression is more than the marketed available clomfranil tablet, and may be linked to its rapid disintegration and bypassing of systemic portal circulation. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
26. RIPK1 in Liver Parenchymal Cells Limits Murine Hepatitis during Acute CCl 4 -Induced Liver Injury.
- Author
-
Hameed, Huma, Farooq, Muhammad, Vuillier, Céline, Piquet-Pellorce, Claire, Hamon, Annaïg, Dimanche-Boitrel, Marie-Thérèse, Samson, Michel, and Le Seyec, Jacques
- Subjects
- *
LIVER cells , *LIVER injuries , *HEPATITIS , *CARBON tetrachloride , *RECEPTOR-interacting proteins , *CELL death - Abstract
Some life-threatening acute hepatitis originates from drug-induced liver injury (DILI). Carbon tetrachloride (CCl4)-induced acute liver injury in mice is the widely used model of choice to study acute DILI, which pathogenesis involves a complex interplay of oxidative stress, necrosis, and apoptosis. Since the receptor interacting protein kinase-1 (RIPK1) is able to direct cell fate towards survival or death, it may potentially affect the pathological process of xenobiotic-induced liver damage. Two different mouse lines, either deficient for Ripk1 specifically in liver parenchymal cells (Ripk1LPC-KO) or for the kinase activity of RIPK1 (Ripk1K45A, kinase dead), plus their respective wild-type littermates (Ripk1fl/fl, Ripk1wt/wt), were exposed to single toxic doses of CCl4. This exposure led in similar injury in Ripk1K45A mice and their littermate controls. However, Ripk1LPC-KO mice developed more severe symptoms with massive hepatocyte apoptosis as compared to their littermate controls. A pretreatment with a TNF-α receptor decoy exacerbated liver apoptosis in both Ripk1fl/fl and Ripk1LPC-KO mice. Besides, a FasL antagonist promoted hepatocyte apoptosis in Ripk1fl/fl mice but reduced it in Ripk1LPC-KO mice. Thus, the scaffolding properties of RIPK1 protect hepatocytes from apoptosis during CCl4 intoxication. TNF-α and FasL emerged as factors promoting hepatocyte survival. These protective effects appeared to be independent of RIPK1, at least in part, for TNF-α, but dependent on RIPK1 for FasL. These new data complete the deciphering of the molecular mechanisms involved in DILI in the context of research on their prevention or cure. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
27. Prunus armeniaca Gum-Alginate Polymeric Microspheres to Enhance the Bioavailability of Tramadol Hydrochloride: Formulation and Evaluation.
- Author
-
Noureen, Shazia, Noreen, Sobia, Ghumman, Shazia Akram, Batool, Fozia, Hameed, Huma, Hasan, Sara, Noreen, Fozia, Elsherif, Mervat A., and Bukhari, Syed Nasir Abbas
- Subjects
APRICOT ,TRAMADOL ,SODIUM alginate ,SELF-healing materials ,MICROSPHERES ,BIOAVAILABILITY ,LABORATORY mice ,THERMAL analysis - Abstract
Combinations of polymers can improve the functional properties of microspheres to achieve desired therapeutic goals. Hence, the present study aimed to formulate Prunus armeniaca gum (PAG) and sodium alginate microsphere for sustained drug release. Blended and coated microspheres were prepared using the ionotropic gelation technique. The effect of polymer concentration variation was studied on the structural and functional properties of formulated microspheres. FTIR, XRD, and thermal analysis were performed to characterize the microspheres. All the formulations were well-formed spherical beads having an average diameter from 579.23 ± 07.09 to 657.67 ± 08.74 μm. Microspheres entrapped drugs within the range 65.86 ± 0.26–83.74 ± 0.79%. The pH-dependent swelling index of coated formulations was higher than blended. FTIR spectra confirmed the presence of characteristic peaks of entrapped Tramadol hydrochloride showing no drug-polymer interaction. In vitro drug release profile showed sustained release following the Korsmeyer-Peppas kinetic model with an R
2 value of 0.9803–0.9966. An acute toxicology study employing the oral route in Swiss albino mice showed no signs of toxicity. It can be inferred from these results that blending PAG with sodium alginate can enhance the stability of alginate microspheres and improve its drug release profile by prolonging the release time. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
28. Synthesis of pH-Sensitive Cross-Linked Basil Seed Gum/Acrylic Acid Hydrogels by Free Radical Copolymerization Technique for Sustained Delivery of Captopril.
- Author
-
Ghumman, Shazia Akram, Noreen, Sobia, Hameed, Huma, Elsherif, Mervat A., Shabbir, Ramla, Rana, Mavra, Junaid, Kashaf, and Bukhari, Syed Nasir Abbas
- Subjects
ACRYLIC acid ,HYDROGELS ,COPOLYMERIZATION ,CAPTOPRIL ,HYDROGEN-ion concentration - Abstract
The pH-sensitive polymeric matrix of basil seed gum (BSG), with two different monomers, such as acrylic acid (AA) and N, N-Methylene-bis-acrylamide (MBA), was selected to use in hydrogels preparation through a free radical copolymerization technique using potassium per sulfate (KPS) as a cross linker. BSG, AA and MBA were used in multiple ratios to investigate the polymer, monomer and initiator effects on swelling properties and release pattern of captopril. Characterization of formulated hydrogels was done by FTIR, DSC/TGA, XRD and SEM techniques to confirm the stability. The hydrogels were subjected to a variety of tests, including dynamic swelling investigations, drug loading, in vitro drug release, sol–gel analyses and rheological studies. FTIR analysis confirmed that after the polymeric reaction of BSG with the AA monomer, AA chains grafted onto the backbone of BSG. The SEM micrographs illustrated an irregular, rough, and porous form of surface. Gel content was increased by increasing the contents of polymeric gum (BSG) with monomers (AA and MBA). Acidic and basic pH effects highlighted the difference between the swelling properties with BSG and AA on increasing concentration. Kinetic modelling suggested that Korsmeyer Peppas model release pattern was followed by the drug with the non-Fickian diffusion mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
29. Preparation and characterization of pH-responsive ionic crosslinked microparticles of mercaptopurine to target ulcerative colitis.
- Author
-
Hameed, Huma, Rehman, Khurram, Hameed, Anam, and Qayuum, Abdul
- Subjects
- *
ULCERATIVE colitis , *PHARMACOKINETICS , *SODIUM tripolyphosphate , *ETHYLCELLULOSE , *CASEINS , *SCANNING electron microscopy - Abstract
The objective of this study was the preparation of ionically crosslinked 6-mercaptopurine (6-MP) monohydrate microparticles through preparing polyelectrolyte complexes of drug and polymers. Polymers such as chitosan, casein, and carrageenan were used to prepare crosslinked microparticles, and sodium tripolyphosphate was used as crosslinker. Microparticles were characterized for their flow behavior, compressibility, percentage yield, micromeritic, and entrapment efficiency. Scanning electron microscopy was conducted to understand the surface morphology of the microparticles, and the result was correlated with the swelling index and percentage drug release. Mathematical modeling of drug release in order to determine the drug release kinetics was also determined to understand the mechanism involving the release of 6-mercaptopurine from the microparticles. The ionic crosslinked microparticles were in the range of 664 μm–798 μm particle size having good flow and compressibility properties with percentage yield were found to be from 77.5% to 87.5% in range. The entrapment efficiency for the formulations were found to be from 63.5% to 83.5%, with MCP-5 gave maximum entrapment efficiency of 83.5%. In vitro swelling and drug release studies were in accordance with the polymer properties following zero-order model with super-case transport II. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
30. Questioning the RIPK1 kinase activity involvement in acetaminophen-induced hepatotoxicity in mouse.
- Author
-
Hameed, Huma, Farooq, Muhammad, Piquet-Pellorce, Claire, Hamon, Annaïg, Samson, Michel, and Le Seyec, Jacques
- Subjects
- *
ACETAMINOPHEN , *HEPATOTOXICOLOGY , *MICE , *CELL death - Abstract
[Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
31. Comparative Analysis of Topiramate with Amitriptyline in Prophylaxis of Migraine without Aura Among Outpatients Visiting Tertiary Care Hospital.
- Author
-
Khan, Wahaj Ul Hassan, Farid, Ummarah Zafar, Hameed, Huma, Naseer, Umer, Gilani, Atifa, and Ali, Kamran
- Subjects
MIGRAINE aura ,AMITRIPTYLINE ,TOPIRAMATE ,TERTIARY care ,COMPARATIVE studies - Abstract
Objective: To ascertain the better drug between Topiramate and Amitriptyline for the prevention of migraines without aura among patients of the adult age group visiting the tertiary care hospital. Study Design: Comparative cross-sectional study. Place and Duration of Study: Combined Military Hospital, Malir Cantt Pakistan, from May to Oct 2022. Methodology: Two hundred seventy individuals visiting the Outpatient Department of the tertiary care hospital were included in the study who were suffering from migraines without aura. They were randomly assigned to groups of 135 each, which were to receive Topiramate and Amitriptyline, respectively. After informed consent, their treatment was started with monthly follow-ups. Data was collected in the form of a questionnaire derived from the Migraine Disability Assessment Questionnaire (MIDAS), Headache Impact Test (HIT-6) and Migraine Specific Quality of Life Questionnaire (MSQ 2.1), both at the start of treatment and later at the end of the study. Results: Two hundred seventy individuals were included in the study. It comprised 162(60%) females and 108(40%) males. Their mean age was 36.51±9.77 years. There was a significant improvement in migraine symptoms among topiramate and amitriptyline groups (p-value<0.001). There was no significant difference in the efficacy of Topiramate and Amitriptyline (p-value=0.411) Conclusion: There was no significant difference in the efficacy of Topiramate and Amitriptyline in the prevention therapy of migraine. [ABSTRACT FROM AUTHOR]
- Published
- 2023
32. DIAGNOSTIC ACCURACY OF QUANTITATIVE WASHOUT CALCULATED ON TRIPHASIC CT SCAN FOR DIAGNOSIS OF HEPATOCELLULAR CARCINOMA KEEPING HISTOPATHOLOGY AS GOLD STANDARD.
- Author
-
Hameed, Huma, Nafees, Muhammad, and Sameeuddin, Syed
- Subjects
- *
LIVER cancer , *HISTOPATHOLOGY , *COMPUTED tomography , *GOLD standard , *CROSS-sectional method - Abstract
Objective: To determine the diagnostic accuracy of quantitative washout calculated on Triphasic CT scan for diagnosis of hepatocellular carcinoma keeping histopathology as gold standard. Study Design: Descriptive, cross-sectional validation study. Place and Duration of Study: Armed Forces Institute of Radiology and Imaging Rawalpindi, from Feb 2016 to Aug 2016. Material and Methods: A total of 132 patients of either sex with age in range 15-75 years diagnosed to have focal liver lesion on ultrasonography were included. Patients in whom focal lesion was cyst or abscess, patients with renal failure, pregnancy or known sensitivity to contrast agents were excluded. All the patients then underwent Triphasic CT scan to calculate quantitative washout on delayed phase. The lesion was diagnosed as HCC if percent attenuation ratio was >107. The results were later correlated with histopathology findings. Results: Mean age was 49.75 ± 15.18 years. Out of 132 patients, 86 (65.15%) were males and 46 (34.85%) were females with ratio of 2:1. In Triphasic CT scan positive patients, 78 were True Positive and 09 were False Positive. Among 45, Triphasic CT scan negative patients, 07 were False Negative where as 38 were True Negative. Overall sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy of quantitative washout calculated on Triphasic CT scan for diagnosis of hepatocellular carcinoma was 91.76%, 80.85%, 89.66%, 84.44% and 87.88% respectively. Conclusion: This study concluded that quantitative washout calculated on Triphasic CT scan is a highly sensitive and accurate non-invasive modality for diagnosis of hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]
- Published
- 2018
33. pH Responsive Abelmoschus esculentus Mucilage and Administration of Methotrexate: In-Vitro Antitumor and In-Vivo Toxicity Evaluation.
- Author
-
Noreen, Sobia, Hasan, Sara, Ghumman, Shazia Akram, Bukhari, Syed Nasir Abbas, Ijaz, Bushra, Hameed, Huma, Iqbal, Huma, Aslam, Afeefa, Elsherif, Mervat Abdelaziz Mohamed, Noureen, Shazia, and Ejaz, Hasan
- Subjects
OKRA ,TOXICITY testing ,MUCILAGE ,METHOTREXATE ,ANTINEOPLASTIC agents ,POLYSACCHARIDES - Abstract
The rapid progression in biomaterial nanotechnology apprehends the potential of non-toxic and potent polysaccharide delivery modules to overcome oral chemotherapeutic challenges. The present study is aimed to design, fabricate and characterize polysaccharide nanoparticles for methotrexate (MTX) delivery. The nanoparticles (NPs) were prepared by Abelmoschus esculentus mucilage (AEM) and chitosan (CS) by the modified coacervation method, followed by ultra-sonification. The NPs showed much better pharmaceutical properties with a spherical shape and smooth surface of 213.4–254.2 nm with PDI ranging between 0.279–0.485 size with entrapment efficiency varying from 42.08 ± 1.2 to 72.23 ± 2.0. The results revealed NPs to possess positive zeta potential and a low polydispersity index (PDI). The in-vitro drug release showed a sustained release of the drug up to 32 h with pH-dependence. Blank AEM -CS NPs showed no in-vivo toxicity for a time duration of 14 days, accompanied by high cytotoxic effects of optimized MTX loaded NPs against MCF-7 and MD-MBA231 cells by MTT assay. In conclusion, the findings advocated the therapeutic potential of AEM/CS NPs as an efficacious tool, offering a new perspective for pH-responsive routing of anticancer drugs with tumor cells as a target. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
34. Switching to Regular Diet Partially Resolves Liver Fibrosis Induced by High-Fat, High-Cholesterol Diet in Mice.
- Author
-
Farooq, Muhammad, Hameed, Huma, Dimanche-Boitrel, Marie-Thérèse, Piquet-Pellorce, Claire, Samson, Michel, and Le Seyec, Jacques
- Abstract
The globally prevalent disease, non-alcoholic steatohepatitis (NASH), is characterized by a steatotic and inflammatory liver. In NASH patients, tissue repair mechanisms, activated by the presence of chronic liver damage, lead to the progressive onset of hepatic fibrosis. This scar symptom is a key prognostic risk factor for liver-related morbidity and mortality. Conflicting reports discuss the efficiency of dietary interventions on the reversibility of advanced fibrosis established during NASH. In the present study, the effect of dietary interventions was investigated in the outcome of the fibrosis settled in livers of C57BL/6J mice on a high-fat, high-cholesterol diet (HFHCD) for 5 or 12 consecutive weeks. Various clinico-pathological investigations, including a histological analysis of the liver, measurement of plasma transaminases, steatosis and fibrosis, were performed. To assess the effectiveness of the dietary intervention on established symptoms, diseased mice were returned to a standard diet (SD) for 4 or 12 weeks. This food management resulted in a drastic reduction in steatosis, liver injuries, inflammatory markers, hepatomegaly and oxidative stress and a gradual improvement in the fibrotic state of the liver tissue. In conclusion, our results demonstrated that dietary intervention can partially reverse liver fibrosis induced by HFHCD feeding. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.