1. Fecal IgA Levels Are Determined by Strain-Level Differences in Bacteroides ovatus and Are Modifiable by Gut Microbiota Manipulation.
- Author
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Yang, Chao, Mogno, Ilaria, Contijoch, Eduardo J., Borgerding, Joshua N., Aggarwala, Varun, Li, Zhihua, Siu, Sophia, Grasset, Emilie K., Helmus, Drew S., Dubinsky, Marla C., Mehandru, Saurabh, Cerutti, Andrea, and Faith, Jeremiah J. more...
- Abstract
Fecal IgA production depends on colonization by a gut microbiota. However, the bacterial strains that drive gut IgA production remain largely unknown. Here, we assessed the IgA-inducing capacity of a diverse set of human gut microbial strains by monocolonizing mice with each strain. We identified Bacteroides ovatus as the species that best induced gut IgA production. However, this induction varied bimodally across different B. ovatus strains. The high IgA-inducing B. ovatus strains preferentially elicited more IgA production in the large intestine through the T cell-dependent B cell-activation pathway. Remarkably, a low-IgA phenotype in mice could be robustly and consistently converted into a high-IgA phenotype by transplanting a multiplex cocktail of high IgA-inducing B. ovatus strains but not individual ones. Our results highlight the critical importance of microbial strains in driving phenotype variation in the mucosal immune system and provide a strategy to robustly modify a gut immune phenotype, including IgA production. • Bacteroides ovatus strain variation drives either high or low fecal IgA • CD4
+ T cells are critical in B. ovatus -mediated gut IgA production • Cocktails of IgAhigh B. ovatus strains convert mice from low- to high-IgA producers Yang et al. identify that different Bacteroides ovatus strains induce either high or low baseline fecal IgA in mice. Cocktails of IgAhigh B. ovatus strains convert mice from low-IgA to high-IgA producers. This demonstrates a role of microbial strains in immune variation and a microbiota-based immune modulation strategy. [ABSTRACT FROM AUTHOR] more...- Published
- 2020
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