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Wheat (Triticum aestivum L.) is one of the most agriculturally and economically important crops in the world. Wheat fungal diseases are becoming more severe and frequent due to global climate change, threatening wheat yields and security. While fungal diseases such as fusarium head blight, stripe rust, and powdery mildew have been extensively studied, the newly emerged fungal pathogens in wheat are still under-researched. In May 2023, black mold symptoms were observed on wheat spikes in Xinxiang City, Henan Province, China. However, the causal agent of this disease was not known. We employed a combination of morphological examination and molecular techniques to identify the pathogen. The internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (tef1), and actin (act) genes of the fungus were partially sequenced (accession no. OR186209, PQ271633 and PQ271632) and showed 99.59–100% identity with the previously reported Cladosporium cladosporioides, which affects wheat, pokeweed, and black-eyed pea. The pathogenicity of this fungus was confirmed by fulfilling Koch’s postulates. Through a rigorous screening process, we found Simplicillium aogashimaense, Trichothecium roseum, and Bacillus velezensis as effective biocontrol agents, with B. velezensis demonstrating the most potent antagonistic activity against the Cladosporium mold. This discovery showed the potential of B. velezensis as a biocontrol agent for wheat disease management. The findings underscore the importance of the present study in advancing the control of this disease.

Among the Ctenocephalides felis felis-borne pathogens, Bartonella henselae, the main aetiological agent of cat scratch disease (CSD), is of increasing comparative biomedical importance. Despite the importance of B. henselae as an emergent pathogen, prevention of the diseases caused by this agent in cats, dogs and humans mostly relies on the use of ectoparasiticides. A vaccine targeting both flea fitness and pathogen competence is an attractive choice requiring the identification of flea proteins/metabolites with a dual effect. Even though recent developments in vector and pathogen -omics have advanced the understanding of the genetic factors and molecular pathways involved at the tick-pathogen interface, leading to discovery of candidate protective antigens, only a few studies have focused on the interaction between fleas and flea-borne pathogens. Taking into account the period of time needed for B. henselae replication in flea digestive tract, the present study investigated flea-differentially abundant proteins (FDAP) in unfed fleas, fleas fed on uninfected cats, and fleas fed on B. henselae-infected cats at 24 hours and 9 days after the beginning of blood feeding. Proteomics approaches were designed and implemented to interrogate differentially expressed proteins, so as to gain a better understanding of proteomic changes associated with the initial B. henselae transmission period (24 hour timepoint) and a subsequent time point 9 days after blood ingestion and flea infection. As a result, serine proteases, ribosomal proteins, proteasome subunit α-type, juvenile hormone epoxide hydrolase 1, vitellogenin C, allantoinase, phosphoenolpyruvate carboxykinase, succinic semialdehyde dehydrogenase, glycinamide ribotide transformylase, secreted salivary acid phosphatase had high abundance in response of C. felis blood feeding and/or infection by B. henselae. In contrast, high abundance of serpin-1, arginine kinase, ribosomal proteins, peritrophin-like protein, and FS-H/FSI antigen family member 3 was strongly associated with unfed cat fleas. Findings from this study provide insights into proteomic response of cat fleas to B. henselae infected and uninfected blood meal, as well as C. felis response to invading B. henselae over an infection time course, thus helping understand the complex interactions between cat fleas and B. henselae at protein levels.

Background. To study the effects of z-guggulsterone on gastric cancer cell apoptosis and the mechanism related. Materials and Methods. Human gastric tumor SGC-7901 cells and GES-1 normal epithelial cells were treated with z-guggulsterone (0–75 μM) for 24 h. MTT assay was applied to evaluate cell proliferation. Flow cytometry and Hoechst staining were used to assess cell apoptosis. Western blotting was applied to evaluate FXR, small heterodimer partner (SHP), Bcl-2, and Bax protein expression. ELISA was applied to gain the levels of active caspase-3 and the contents of TNF-α, TGF-β1, and VEGF. Results. The expression levels of FXR and SHP were higher in tumor cells than in normal epithelial cells. Inhibition of FXR signaling with z-guggulsterone dose-dependently inhibited SGC-7901 cell proliferation and promoted SGC-7901 cell apoptosis. Bcl-2 protein expression was significantly decreased, and active caspase-3 and Bax protein expression was increased in SGC-7901 cells incubated with z-guggulsterone. The content of TNF-α was significantly increased, and the contents of VEGF and TGF-β1 were decreased in SGC-7901 cells incubated with z-guggulsterone. Conclusions. Inhibition of FXR signaling with z-guggulsterone induced anticancer effects in SGC-7901 cells by decreasing cell proliferation and promoting apoptosis. Z-guggulsterone induced cell apoptosis through the mitochondria-dependent pathway.

Along with the development of recombinant DNA technology and the deepening of genetic engineering vaccine research, plant genetic engineering has become the focus of vaccine research gradually, whereas the defects of traditional vaccine have appeared day by day. So far, the vaccines expressed in transgenic plants mainly include Escherichia coli heat-labile enterotoxn (Etx) subunit B, Hepatitis B Surface Antigen (HBsAg), Norwalk virus capsid protein (NVCP), foot-and-mouth disease (FMD), rabies virus glycoprotein (RVG), Streptococcus mutans surface proteins (SmSP) and AIDS viral antigens (AVA). In comparison with traditonal vaccines, transgenic plant vaccine is cheaper, safer, more efficient, easier for transportation and storage. This paper gives a review on the research progress of plant genetic engineering vaccines, and proposes the issues encountering the development process and corresponding countermeasures, as well as its prospect. [ABSTRACT FROM AUTHOR]