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Your search keyword '"I. Herbst"' showing total 2 results
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2 results on '"I. Herbst"'

1. Unexpected death of a 9-year-old girl

Author

Celeste I, Herbst, John J, Dempers, and S Dan, Zaharie

Subjects
Fatal Outcome, Brain Stem Neoplasms, Humans, Female, Astrocytoma, Child, Cases of the Month
Published
2018

2. Improvement of HLA class I and class II PCR-SSP typing by using timed-release activity of DNA polymerase

Author

W. Stangel, H.‐Y. Chen, I. Herbst‐Kiene, I. Seibert, D.-F. Chen, and L.T. Pastucha

Subjects
Time Factors, DNA polymerase, Immunology, Human leukocyte antigen, Polymerase Chain Reaction, Biochemistry, law.invention, law, Genetics, Immunology and Allergy, Taq Polymerase, Typing, Allele, Polymerase chain reaction, DNA Primers, biology, Histocompatibility Testing, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Multiple displacement amplification, General Medicine, Virology, Molecular biology, biology.protein, Reagent Kits, Diagnostic, Hot start PCR
Abstract

Variable amounts of non-specific amplification may occur in HLA PCR-SSP typing, and this can be significantly reduced by the use of AmpliTaq Gold. In an effort to achieve an optimal balance between specificity and efficiency of the PCR amplification for both HLA alleles and the internal control, we designed a system of timed-release activity by combining two different Taq DNA polymerases. The reaction was started at a relatively low level of enzyme activity and as thermal cycling progressed more and more Ampli-Taq Gold was slowly activated for the reaction to continue. We applied this system to all routine HLA PCR-SSP typing. The number of repeat typings due to non-specific amplification and/or amplification failure of the internal control was remarkably reduced.

Published
1998
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