1. A promising natural product, pristimerin, results in cytotoxicity against breast cancer stem cells in vitro and xenografts in vivo through apoptosis and an incomplete autopaghy in breast cancer
- Author
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Ayça Üvez, Mattia Mori, Konstantinos Dimas, Cinzia Ingallina, Pınar Alper, Chrisiida Tsimplouli, Ilaria D'Acquarica, Nazlihan Aztopal, Merve Erkisa, Elif Ilkay Armutak, Bruno Botta, Bulent Ozpolat, Simone Berardozzi, Evangelia Sereti, Didem Karakas, Buse Cevatemre, Ebru Gürel Gürevin, Engin Ulukaya, Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Cevatemre, Buse, Erkısa, Merve, Aztopal, Nazlıhan, Karakaş, Didem, Alper, Pınar, AHD-2050-2022, AAM-1001-2020, L-6687-2018, L-6682-2018, İstinye Üniversitesi, Rektörlük, Didem Karakaş / 0000-0002-3781-6834, Engin Ulukaya / 0000-0003-4875-5472, Merve Erkısa Genel / 0000-0002-3127-742X, Pınar Alper / 0000-0001-9631-3551, Erkisa Genel, Merve, Karakaş Zeybek, Didem, Ulukaya, Engin, Didem Karakaş / L-6682-2018, Engin Ulukaya / K-5792-2018, Merve Erkısa Genel / AAM-1001-2020, Pınar Alper / DUL-1586-2022, Didem Karakaş / 56422040600, Engin Ulukaya / 6602927353, Merve Erkısa Genel / 57126208900, and Pınar Alper / 57197858774
- Subjects
0301 basic medicine ,Palladium(ıı) ,Protein bcl 2 ,Unclassified drug ,Mouse ,Proliferation ,Enzyme activation ,Apoptosis ,Antiproliferative activity ,IC50 ,MCF-7 cell line ,Experimental mammary neoplasm ,Cell vacuole ,Animal tissue ,Metastasis ,Western blotting ,Biological product ,Unfolded protein response ,Degradation ,Mice ,In vivo study ,Breast cancer ,0302 clinical medicine ,Antineoplastic agents ,Cytotoxic T cell ,Flow cytometry ,Priority journal ,Caspase 7 ,education.field_of_study ,Signaling pathway ,Caspase 3 ,Chemistry ,Neoplastic stem cells ,Antineoplastic agent ,Protein cleavage ,Drug screening ,Sesquiterpene ,Pristimerin ,Maytenus ,030220 oncology & carcinogenesis ,Sequestosome 1 ,cytoplasmic vacuolation ,endoplasmic reticulum stress ,Female ,triterpenoid ,Stem cell ,Pentacyclic Triterpenes ,Animal cell ,Drug mechanism ,Xenograft model antitumor assays ,Human ,Drug cytotoxicity ,Programmed cell death ,Microtubule associated protein ,Microtubule associated protein 1A 1B light chain 3B ,mammosphere ,Population ,Article ,03 medical and health sciences ,Cancer stem cell ,Autophagy ,Animals ,Humans ,Animal model ,Drug dose comparison ,Biological products ,Animal experiment ,ATPase activity assay ,education ,Antineoplastic activity ,Pharmacology ,Pharmacology & pharmacy ,Animal ,Carcinoma ,In vitro study ,Tumor cell line ,Nonhuman ,Triterpenes ,Mammary neoplasms, experimental ,Drug effect ,Drug efficacy ,Cell line, tumor ,030104 developmental biology ,Human cell ,Doxorubicin ,Cancer cell ,Cancer research ,Protein expression ,Tumor xenograft ,Triterpene ,Controlled study ,Phosphatidylinositol 3 kinase - Abstract
WOS: 000428102600047 Several natural products have been suggested as effective agents for the treatment of cancer. Given the important role of CSCs (Cancer Stem Cells) in cancer, which is a trendy hypothesis, it is worth investigating the effects of pristimerin on CSCs as well as on the other malignant cells (MCF-7 and MDA-MB-231) of breast cancer. The anti-growth activity of pristimerin against MCF-7 and MCF-7s (cancer stem cell enriched population) cells was investigated by real time viability monitorization (xCELLigence System (R)) and ATP assay, respectively. Mode of cell death was evaluated using electron and fluorescence microscopies, western blotting (autophagy, apoptosis and ER-stress related markers) and flow cytometry (annexin-V staining, caspase 3/7 activity, BCL-2 and PI3K expressions). Pristimerin showed an anti-growth effect on cancer cells and cancer stem cells with IC50 values ranging at 0.38-1.75 mu M. It inhibited sphere formation at relatively lower doses (
- Published
- 2018
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