1. High-resolution whole organ imaging using two-photon tissue cytometry.
- Author
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Timothy Ragan, Jeremy D. Sylvan, Ki Hean Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, and Peter T. C. So
- Subjects
THREE-dimensional imaging ,CYTOMETRY ,LABORATORY mice ,HEART - Abstract
Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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