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The purpose of the article is to highlight the updated theoretical approaches to the essence, significance and evaluation of the effectiveness of the technology transfer process (taking into account such a stage of transfer as construction-technological support of the processes of production of new equipment for industrial enterprises) within the terms of the chosen management model (in particular, the authors’ SEE-management) for further development of recommendations for their practical application in the context of innovative development of industrial enterprises. The scientific novelty of the article is the delineation of the latest approaches to the application of models of effectiveness components with relevant indicators therein for determining, measuring and evaluating the effectiveness of the process of technology transfer of industrial enterprises in the context of process, system, and holistic approaches with an emphasis on the possibility of using these models as proven positive experience over more than 25 years. It is proved that as a useful tool for SEE-management of the effectiveness of the technology transfer process can serve the authors’ performance matrix for visualizing possible adjustments to the indices of its components. It is emphasized that the results of calculations based on specific data of industrial enterprises according to the models of the components of the effectiveness of the technology transfer process can be taken as a basis for making a managerial decision on the feasibility of implementing a certain scientific development. It is specified that the use of a system of indicators in models of effectiveness components for the process of technology transfer opens the possibility to implement in practice an integrated approach to the analytical, predictive, and other components of management (in particular, SEE-management) of technology transfer, starting with making a decision on the feasibility of transfer and ending with evaluating its effectiveness.

We present results on simultaneous observations of Class I methanol masers at 25, 36, and 44 GHz towards 22 Galactic targets carried out with the Effelsberg 100-m telescope. The study investigates relations between the hyperfine (HF) structure of the torsion–rotation transitions in CH |$_3$| OH and maser activity. By analysing the radial velocity shifts between different maser lines together with the patterns of the HF structure based on laboratory measurements and quantum-chemical calculations, we find that in any source only one specific HF transition forms the maser emission and that this transition changes from source to source. The physical conditions leading to this selective behaviour are still unclear. Using accurate laboratory rest frequencies for the 25 GHz transitions, we have refined the centre frequencies for the HF multiplets at 36, 44, and 95 GHz: |$f_{\scriptscriptstyle 36} = (36169.2488\pm 0.0002_{\scriptscriptstyle \rm stat} \pm 0.0004_{\scriptscriptstyle \rm sys})$|  MHz. |$f_{\scriptscriptstyle 44} = (44069.4176\pm 0.0002_{\scriptscriptstyle \rm stat} \pm 0.0004_{\scriptscriptstyle \rm sys})$|  MHz, and |$f_{\scriptscriptstyle 95} = (95169.4414\pm 0.0003_{\scriptscriptstyle \rm stat} \pm 0.0004_{\scriptscriptstyle \rm sys})$|  MHz. Comparison with previous observations of 44 GHz masers performed 6–10 yr ago with a Korean 21-m Korean Very Long Baseline Interferometry Network telescope towards the same targets confirms the kinematic stability of Class I maser line profiles during this time interval and reveals a systematic radial velocity shift of |$0.013\pm 0.005$|  km s |$^{-1}$| between the two telescopes. [ABSTRACT FROM AUTHOR]

The process of stone formation in the human body remains incompletely understood, which requires clinical and laboratory studies and the formulation of a new endogenous, nanotechnological concept of the mechanism of origin and formation of crystallization centers. Previously, the mechanism of sialolithiasis was considered a congenital disease associated with the pathology of the ducts in the structure of the glands themselves. To date, such morphological changes of congenital nature can be considered from the position of the intrauterine formation of endogenous bacterial infections complicated by the migration of antigenic structures initiating the formation of crystallization centers. The present work is devoted to the study of the morphology and composition of stones obtained as a result of surgical interventions for sialolithiasis. Presumably, nanoparticles of metals and other chemical compounds can be structural components of crystallization centers or incorporated into the conditions of chronic endogenous inflammation and the composition of antigenic structures, in complexes with protein and bacterial components. X-ray microtomography, X-ray fluorescence analysis, scanning transmission electron microscopy and microanalysis, mass spectrometry, and Raman spectroscopy were used to study the pathogenesis of stone formation. Immunoglobulins (Igs) of classes A and G, as well as nanoparticles of metals Pb, Fe, Cr, and Mo, were found in the internal structure of the stones. The complex of antigenic structures was an ovoid calcified layered matrix of polyvid microbial biofilms, with the inclusion of metal nanoparticles and chemical elements, as well as immunoglobulins. The obtained results of clinical and laboratory studies allow us to broaden the view on the pathogenesis of stone formation and suggest that the occurrence of the calcification of antigenic structures may be associated with the formation of IgG4-associated disease. [ABSTRACT FROM AUTHOR]

The SSB protein of Escherichia coli functions to bind single-stranded DNA wherever it occurs during DNA metabolism. Depending upon conditions, SSB occurs in several different binding modes. In the course of its function, SSB diffuses on ssDNA and transfers rapidly between different segments of ssDNA. SSB interacts with many other proteins involved in DNA metabolism, with 22 such SSB-interacting proteins, or SIPs, defined to date. These interactions chiefly involve the disordered and conserved C-terminal residues of SSB. When not bound to ssDNA, SSB can aggregate to form a phase-separated biomolecular condensate. Current understanding of the properties of SSB and the functional significance of its many intermolecular interactions are summarized in this review. [ABSTRACT FROM AUTHOR]

A new platform has been developed to facilitate the production of biologically active proteins and peptides in Escherichia coli. The platform includes an N‐terminal self‐associating L6KD peptide fused to the SUMO protein (small ubiquitin‐like protein modifier) from the yeast Saccharomyces cerevisiae, which is known for its chaperone activity. The target proteins are fused at the C termini of the L6KD‐SUMO fusions, and the resulting three‐component fusion proteins are synthesized and self‐assembled in E. coli into so‐called active inclusion bodies (AIBs). In vivo, the L6KD‐SUMO platform facilitates the correct folding of the target proteins and directs them into AIBs, greatly simplifying their purification. In vitro, the platform facilitates the effective separation of AIBs by centrifugation and subsequent target protein release using SUMO‐specific protease. The properties of the AIBs were determined using five proteins with different sizes, folding efficiencies, quaternary structure, and disulfide modifications. Electron microscopy shows that AIBs are synthesized in the form of complex fibrillar structures resembling "loofah sponges" with unusually thick filaments. The obtained results indicate that the new platform has promising features and could be developed to facilitate the synthesis and purification of target proteins and protein complexes without the use of renaturation. [ABSTRACT FROM AUTHOR]

We present results on numerical calculations of the sensitivity coefficients, Q μ, of microwave molecular transitions in 13CH3OH and |${\rm CH_3\, ^{18}OH}$| to the hypothetical variation in the fundamental physical constant μ – the electron-to-proton mass ratio. The invariability of μ in time and space is one of the basic assumptions of the Standard Model of particle physics which can be tested at cosmological scales by means of astronomical observations in the Galaxy and external galaxies. Our calculations show that these two methanol isotopologues can be utilized for such tests since their microwave transitions from the frequency interval 1–100 GHz exhibit a large spread in Q μ values which span a range of −109 ≲ Q μ ≲ 78. We show that the thermal emission lines of 13CH3OH observed in the star-forming region NGC 6334I constrain the variability of μ at a level of 3 × 10−8 (1σ), which is in line with the most stringent upper limits obtained previously from observations of methanol (CH3OH) and other molecules in the Galaxy. [ABSTRACT FROM AUTHOR]

Most modern calculations of many-electron atoms use basis sets of atomic orbitals. An accurate account for electronic correlations in heavy atoms is a very difficult computational problem, and an optimization of the basis sets can reduce computational costs and increase final accuracy. Here, we propose a simple differential ansatz to form virtual orbitals from the Dirac–Fock orbitals of the core and valence electrons. We use basis sets with such orbitals to calculate different properties in Cs including hyperfine structure constants and QED corrections to the valence energies and to the E 1 transition amplitudes. [ABSTRACT FROM AUTHOR]