1. Molecular cross-talk between the transcription, translation, and nonsense-mediated decay machineries.
- Author
-
Iborra, Francisco J., Escargueil, Alexandre E., Kwek, Kon Y., Akoulitchev, Alexandre, and Cook, Peter R.
- Subjects
GENETIC transcription ,GENETIC translation ,CELL nuclei ,ENZYMES ,RNA polymerases ,MICROSCOPY - Abstract
It is widely believed that translation occurs only in the cytoplasm of eukaryotes, but recent results suggest some takes place in nuclei, coupled to transcription. Support for this heterodoxy comes from studies of the nonsensemediated decay (NMD) pathway; this pathway probably uses ribosomes to proofread messenger RNAs. We find components of the machineries involved in transcription, translation and NMD colocalise, interact and copurify, and that interactions between them are probably mediated by the C-terminal domain of the catalytic subunit of RNA polymerase H. These results are simply explained if the NMD machinery uses nuclear ribosomes to translate - and so proofread - newly made transcripts; then, faulty transcripts and any truncated peptides produced by nuclear translation would be degraded. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF