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4. Identification of CRISPR/Cas9 knockout targets and tissue expression analysis of circadian clock genes <italic>GmLNK1/2</italic>, <italic>GmRVE4/8</italic>, and <italic>GmTOC1 </italic>in soybean

Author

GAN, Zhuo-Ran, primary, DONG, Li-Dong, additional, HOU, Zhi-Hong, additional, LU, Si-Jia, additional, LI, Hai-Yang, additional, CHENG, Qun, additional, LI, Yong-Li, additional, LIU, Bao-Hui, additional, and SHI, Wen-Qian, additional

Published
2020
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15. RIP3 deficiency protects against traumatic brain injury (TBI) through suppressing oxidative stress, inflammation and apoptosis: Dependent on AMPK pathway

Author

Liu, Zai-Ming, primary, Chen, Qian-Xue, additional, Chen, Zhi-Biao, additional, Tian, Dao-Feng, additional, Li, Ming-Chang, additional, Wang, Jun-Min, additional, Wang, Long, additional, Liu, Bao-Hui, additional, Zhang, Shen-Qi, additional, Li, Fei, additional, Ye, Hui, additional, and Zhou, Long, additional

Published
2018
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18. Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines

Author

Yang, Ji-An, Li, Ji-Qiang, Shao, Ling-Min, Yang, Qian, Liu, Bao-Hui, Wu, Ting-Feng, Wu, Peng, Yi, Wei, and Chen, Qian-Xue

Subjects
Original Article
Abstract

Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells.

Published
2015

24. Identification of additional QTLs for flowering time by removing the effect of the maturity gene E1in soybean

Author

LU, Si-jia, LI, Ying, WANG, Jia-lin, NAN, Hai-yang, CAO, Dong, LI, Xiao-ming, SHI, Dan-ning, FANG, Chao, SHI, Xin-yi, YUAN, Xiao-hui, Abe, Jun, LIU, Bao-hui, and KONG, Fan-jiang

Abstract

The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines (RILs) into two sub-populations: one with the E1allele and another with the e1nlallele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two (qFT-B1and qFT-H) in RILs with the E1allele and one (qFT-J-2) in the RILs with the e1nlallele, respectively. The three QTLs, qFT-B1, qFT-Hand qFT-J-2were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.

Published
2016
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25. Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines.

Author

Yang JA, Li JQ, Shao LM, Yang Q, Liu BH, Wu TF, Wu P, Yi W, and Chen QX

Abstract

Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells.

Published
2015

26. LRIG1 enhances the radiosensitivity of radioresistant human glioblastoma U251 cells via attenuation of the EGFR/Akt signaling pathway.

Author

Yang JA, Liu BH, Shao LM, Guo ZT, Yang Q, Wu LQ, Ji BW, Zhu XN, Zhang SQ, Li CJ, and Chen QX

Subjects
Apoptosis, Cell Line, Tumor, Cell Survival, ErbB Receptors genetics, ErbB Receptors metabolism, Humans, Membrane Glycoproteins genetics, Radiation Tolerance, Radiation, Ionizing, Brain Neoplasms radiotherapy, Gene Expression Regulation, Neoplastic, Glioblastoma radiotherapy, Membrane Glycoproteins metabolism, Signal Transduction
Abstract

The radiotherapy as a local and regional modality is widely applied in treatment of glioma, but most glioblastomas are commonly resistant to irradiation treatment. It remains challengeable to seek out efficient strategies to conquer the resistance of human glioblastoma cells to radiotherapy. Leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1) is a newly discovered tumor suppressor which involved in regulation of chemosensitivity in various human cancer cells. In the present study, we established a radioresistant U251 cell line (U251R) to investigate the role of LRIG1 in regulation of radiosensitivity in human glioblastoma cells. Significantly decreased expression level of LRIG1 and enhanced expression of EGFR and phosphorylated Akt were detected in U251R cells compared with the parental U251 cells. U251R cells exhibited an advantage in colony formation ability, which accompanied by remarkably reduced X-ray-induced γ-H2AX foci formation and cell apoptosis. LRIG1 overexpression significantly inhibited the colony formation ability of U251R cells and obviously enhanced X-ray-inducedγ-H2AX foci formation and cell apoptosis. In addition, up-regulated expression of LRIG1 suppressed the expression level of EGFR and phosphorylated Akt protein. Our results demonstrated that LRIG1 expression was related to the radiosensitivity of human glioblastoma cells and may play an important role in the regulation of cellular radiosensitivity of human glioblastoma cells through the EGFR/Akt signaling pathway.

Published
2015

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