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Objective To investigate the relationship between serum manganese (Mn) levels and non-alcoholic fatty liver disease (NAFLD) in individuals of different genders. Methods Participants aged 18 years or older from the National Health and Nutrition Examination Survey (NHANES) database between 2017 and 2018 were selected, excluding those who lacked ultrasonic liver transient elastography data, serum Mn data, and individuals with hepatitis B, hepatitis C, or significant alcohol consumption. The included population (n=4 294) was divided into NAFLD group (n=2 708) and the non-NAFLD group (n=1 586) according to whether participant had NAFLD or not. Serum Mn levels were quantitatively measured by inductively coupled plasma dynamic reaction cell mass spectrometry. Age, gender, race, marital status, education, family income-to-poverty ratio (FMPIR), body mass index (BMI), smoking, alcohol consumption, diabetes, hypertension, hyperlipidemia, and serum Mn levels were compared between the two groups. Continuous variables were compared by t-tests or Wilcoxon rank-sum tests, while categorical variables were compared by chi-square tests. Males and females were respectively divided into four groups (Q1-Q4) according to their Mn levels by quartile method. Multivariate logistic regression, restricted cubic spline regression analysis, interaction, and subgroup analysis were performed to explore the risk association between serum Mn and NAFLD in different genders. Results Serum Mn level in the NAFLD group was significantly higher than that in the non-NAFLD group［9.06 (7.25, 11.38)μg/L vs 9.33 (7.54, 11.52) μg/L, Z=2.815, P=0.005］. After adjusting for age, race, education, marital status, FMPIR, BMI, smoking, alcohol consumption, diabetes mellitus, hypertension and hyperlipidemia as covariates, male participants in the serum Mn Q3 group (OR=1.575; 95%CI: 1.193-2.087) and female participants in the serum Mn Q4 group (OR=1.725; 95%CI: 1.313-2.269) had significantly increased risks of developing NAFLD when compared to those in the Q1 group (P＜0.01). There was a significant positive dose-response relationship between serum Mn and the risk of NAFLD for both males and females (P＜0.01), with a nonlinear relationship for males (Pnon-linear＜0.01) and a linear relationship for females (Pnon-linear=0.818）. Significant interactions were observed between serum Mn and race in males, and between serum Mn and hypertension in females. Conclusion Serum Mn levels in both males and females are significantly associated with the risk of NAFLD.

With the development of the times, computer, information technology gradually penetrated into all walks of life. Textile and clothing design, production, consumption integration of machine vision, graphic image recognition technology development have become a trend. At present, the intelligence degree of the textile and clothing industry has reached an unprecedented new height. In the production and identification of fabrics, the machine vision gradually replaces the manual work, and realizes the automatic and accurate production line. In the design, manufacturing, consumption and other aspects of clothing, image recognition technology has assumed the historical responsibility. Combined with the Internet of things, cloud computing and other technologies, it has improved the production efficiency and achieved intelligent design and production.

Objective To investigate whether vascular endothelial cells affect the differentiation of epicardial progenitor cells (EpiCs) during coronary development. Methods Highly purified EpiCs were extracted from 30 female and 30 male C57BL/6 mice (7 to 8 weeks old, body weight 25-30 g) and cultured alone or in the presence of human umbilical vein endothelial cells (HUVECs) in a Transwell co-culture system. The expressions of EpiCs markers WT1 and Tbx18 and the smooth muscle cell markers α-SMA and Myh11 were detected using immunofluorescence assay and qRT-PCR. Collagen gel contraction assay was used to assess the contractility of the cultured EpiCs, and ELISA was used to detect the expression of growth factors in the cell culture medium. Results Compared with EpiCs cultured alone, the EpiCs co-cultured with HUVECs exhibited significantly increased expression levels of α-SMA [(1.73±0.85)% vs (34.20±3.38)%, P < 0.01, n=3] and Myh11 [(3.70±1.49)% vs (45.63±4.85)%, P < 0.01, n=3] with obviously enhanced contractile ability. The concentration of basic fibroblast growth factors (bFGFs) in the conditioned medium was also significantly higher in the co-cultured cells than in EpiCs cultured alone (304.96±52.91 vs 87.64±8.78 pg/mL, P < 0.01, n=3). Conclusion In the co-culture system of EpiCs and HUVECs, the production of bFGFs by HUVECs promotes the differentiation of EpiCs into vascular smooth muscle cells, indicating a regulatory mechanism of the development of coronary arteries

Oral English teaching is an important form of cultivating students' English accomplishment and improving their English learning effect. In the context of intelligent education, by giving full play to the advantages of computer network and actively reconstructing the new mode of Oral English teaching, we will, on the basis of optimizing the form of oral English teaching and by integrating high-quality oral English teaching resources, to promote students to participate in oral English learning and speaking practice in a conscious and spontaneous way. Based on the analysis of the existing problems in spoken English teaching, this paper puts forward the construction and implementation of a new spoken English teaching model based on computer network.

Uranium bioleaching has been rapidly developed in China during recent years. However, some problems during bioleaching, such as low uranium leaching efficiency for rocks lacking pyrite, remain to be solved. A granite type uranium deposit from southern China was selected for the study of U bioleaching with addition of pyrite. Experiments using four approaches, including (i) pyrite ores mixing with culture, (ii) pyrite tiled on the surface of ores with culture, (iii) no pyrite ores with culture, and (iv) pyrite ores without culture were examined in this study. Results showed that bacteria can improve U leaching efficiency, particularly by adding pyrite into ore. Approach (i) pyrite ores mixing with culture had the highest U concentration and the highest recovery rate of the four approaches. No significant difference was observed between approaches (ii) pyrite tiled on the surface of ores with culture and (iii) no pyrite ores with culture.