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18. Determination of isoflavone in soybean by ultra performance liquid chromatography-tandem mass spectrometry

Author

LIN Shengjun, LIU Guoping, XUE Rongxuan, LU Liming, WEN Yilei, and HUANG Yingsi

Subjects
genistein, daidzein, glycitein, soybean, isoflavone, Food processing and manufacture, TP368-456, Nutrition. Foods and food supply, TX341-641
Abstract

ObjectiveTo establish a method for the determination of isoflavone in soybean by ultra performance liquid chromatography tandem mass spectrometry with the contents of genistein， daidzein and glycitein as the indexes.MethodsThe samples were extracted by ethanol-water （3+1， V/V）， separated by Acquity UPLC® BEH C18 column（3.0 mm ×150 mm， 1.7 μm） and then eluted by methanol and 0.1% formic acid solution （containing 5 mmol/L ammonium acetate） as mobile phase， detected by UPLC-MS/MS under the electrospray ionization （ESI-） anion ion mode with multiple reaction monitoring mode. Results The linear relationship between genistein and daidzein was good in the range of 5.0-500 μg/L （r>0.995）， the detection limits were 2.7 and 4.0 mg/kg， respectively. The average recoveries at the addition levels of 300.0， 600.0， and 1 200 mg/kg were 89.4% to 102.2% and the relative standard deviations （n=6） were 1.5% to 4.6%. The linear relationship of glycitein was good in the range of 0.5-50.0 μg/L （r>0.995）， the detection limit was 0.6 mg/kg. The average recoveries at the addition levels of 30.0， 60.0， and 120.0 mg/kg were 85.7% to 104.0% and the relative standard deviations （n=6） were 1.3% to 2.8%.ConclusionThe method is simple， sensitive and accurate， which is suitable for the determination of isoflavone in soybean.

Published
2023
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39. Overexpression of CBL-Interacting Protein Kinases 23 Improves Tolerance to Low-Nitrogen Stress in Potato Plants.

Author

Huang, Feiyun, Lu, Yifei, Li, Zi, Zhang, Lang, Xie, Minqiu, Ren, Bi, Lu, Liming, Li, Liqin, and Yang, Cuiqin

Subjects
PROTEIN kinases, PROTEIN overexpression, POTATOES, PLANT growth, ABIOTIC stress, PLANT development
Abstract

CBL-interacting protein kinases (CIPKs) play important regulatory roles in plant growth development and abiotic stress tolerance. However, the biological roles of these genes in response to low-nitrate (LN) stress in potato plants have not been determined. Here, we reported that StCIPK23 was expressed mainly in roots and leaves. StCIPK23 was located mainly in the cell membrane, nucleus, and cytoplasm. Further research suggested that, compared with wild-type (WT) plants, StCIPK23-overexpressing plants were taller and had significantly greater nitrate and ammonium nitrogen contents under LN stress. StCIPK23 overexpression can increase StAT, StNRT2.1, StNR, StGS1-3, and StGOGAT expression levels in StCIPK23 transgenic seedlings compared to those in WT plants under LN stress. The results of yeast two-hybrid and luciferase complementation imaging experiments suggested that StCIPK23 could interact with StCBL3. Real-time reverse transcription–PCR revealed the StCIPK23 expression level peaked at 6 h and the StCBL3 expression level peaked at 9 h in the roots under LN stress. In conclusion, we found that StCIPK23 and StCBL3 form a complex to regulate the expression of key genes in the nitrogen metabolism pathway to improve LN tolerance in potato plants. [ABSTRACT FROM AUTHOR]

Published
2024
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42. Iron ore sintering

Author

Lu, Liming, primary, Ishiyama, Osamu, additional, Higuchi, Takahide, additional, Matsumura, Masaru, additional, and Higuchi, Kenichi, additional

Published
2022
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