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118 results on '"Ludolf Fernanda"'

1. In vitro and in vivo antileishmanial activity of β-acetyl-digitoxin, a cardenolide of Digitalis lanata potentially useful to treat visceral leishmaniasis

Author

Freitas Camila S., Lage Daniela P., Oliveira-da-Silva João A., Costa Rafaella R., Mendonça Débora V.C., Martins Vívian T., Reis Thiago A.R., Antinarelli Luciana M.R., Machado Amanda S., Tavares Grasiele S.V., Ramos Fernanda F., Brito Rory C.F., Ludolf Fernanda, Chávez-Fumagalli Miguel A., Roatt Bruno M., Ramos Gabriela S., Munkert Jennifer, Ottoni Flaviano M., Campana Priscilla R.V., Duarte Mariana C., Gonçalves Denise U., Coimbra Elaine S., Braga Fernão C., Pádua Rodrigo M., and Coelho Eduardo A.F.

Subjects
treatment, β-acetyl-digitoxin, visceral leishmaniasis, drug repositioning, toxicity, miltefosine, Infectious and parasitic diseases, RC109-216
Abstract

Current treatments of visceral leishmaniasis face limitations due to drug side effects and/or high cost, along with the emergence of parasite resistance. Novel and low-cost antileishmanial agents are therefore required. We report herein the antileishmanial activity of β-acetyl-digitoxin (b-AD), a cardenolide isolated from Digitalis lanata leaves, assayed in vitro and in vivo against Leishmania infantum. Results showed direct action of b-AD against parasites, as well as efficacy for the treatment of Leishmania-infected macrophages. In vivo experiments using b-AD-containing Pluronic® F127 polymeric micelles (b-AD/Mic) to treat L. infantum-infected mice showed that this composition reduced the parasite load in distinct organs in more significant levels. It also induced the development of anti-parasite Th1-type immunity, attested by high levels of IFN-γ, IL-12, TNF-α, GM-CSF, nitrite and specific IgG2a antibodies, in addition to low IL-4 and IL-10 contents, along with higher IFN-γ-producing CD4+ and CD8+ T-cell frequency. Furthermore, low toxicity was found in the organs of the treated animals. Comparing the therapeutic effect between the treatments, b-AD/Mic was the most effective in protecting animals against infection, when compared to the other groups including miltefosine used as a drug control. Data found 15 days after treatment were similar to those obtained one day post-therapy. In conclusion, the results obtained suggest that b-AD/Mic is a promising antileishmanial agent and deserves further studies to investigate its potential to treat visceral leishmaniasis.

Published
2021
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2. A clioquinol-containing Pluronic® F127 polymeric micelle system is effective in the treatment of visceral leishmaniasis in a murine model

Author

Tavares Grasiele S.V., Mendonça Débora V.C., Pereira Isabela A.G., Oliveira-da-Silva João A., Ramos Fernanda F., Lage Daniela P., Machado Amanda S., Carvalho Lívia M., Reis Thiago A.R., Perin Luísa, Carvalho Ana Maria R.S., Ottoni Flaviano M., Ludolf Fernanda, Freitas Camila S., Bandeira Raquel S., Silva Alessandra M., Chávez-Fumagalli Miguel A., Duarte Mariana C., Menezes-Souza Daniel, Alves Ricardo J., Roatt Bruno M., and Coelho Eduardo A.F.

Subjects
treatment, visceral leishmaniasis, clioquinol, immune response, delivery systems, miltefosine, Infectious and parasitic diseases, RC109-216
Abstract

A clioquinol (ICHQ)-containing Pluronic® F127 polymeric micelle system (ICHQ/Mic) was recently shown to be effective against Leishmania amazonensis infection in a murine model. In the present study, ICHQ/Mic was tested against L. infantum infection. BALB/c mice (n = 12 per group) were infected with L. infantum stationary promastigotes through subcutaneous injection and, 45 days after challenge, received saline or were treated via the subcutaneous route with empty micelles, ICHQ or ICHQ/Mic. In addition, animals were treated with miltefosine by the oral route, as a drug control. Half of the animals were euthanized 1 and 15 days after treatment, aiming to evaluate two endpoints after therapy, when parasitological and immunological parameters were investigated. Results showed that the treatment using miltefosine, ICHQ or ICHQ/Mic induced significantly higher anti-parasite IFN-γ, IL-12, GM-CSF, nitrite and IgG2a isotype antibody levels, which were associated with low IL-4 and IL-10 production. In addition, a higher frequency of IFN-γ and TNF-α-producing CD4+ and CD8+ T-cells was found in these animals. The parasite load was evaluated in distinct organs, and results showed that the treatment using miltefosine, ICHQ or ICHQ/Mic induced significant reductions in organic parasitism in the treated and infected mice. A comparison between the treatments suggested that ICHQ/Mic was the most effective in inducing a highly polarized Th1-type response, as well as reducing the parasite load in significant levels in the treated and infected animals. Data obtained 15 days after treatment suggested maintenance of the immunological and parasitological responses. In conclusion, ICHQ/Mic could be considered in future studies for the treatment of visceral leishmaniasis.

Published
2020
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3. Urine-based ELISA using a recombinant chimeric protein for the diagnosis of paucibacillary and multibacillary leprosy

Author

Câmara, Raquel S.B., Pereira, Isabela A.G., Espíndola, Geise C., Lage, Daniela P., Silva, Ana L., Freitas, Camila S., Assis, Bárbara P.N., Corrêa, Laís V.A., Moreira, Ricardo L.F., Lyon, Sandra, Silva, Rozana C., Barros, Tiago S., de Oliveira, Ana Laura G., Ludolf, Fernanda, Chávez-Fumagalli, Miguel A., Christodoulides, Myron, Machado-de-Ávila, Ricardo A., Tupinambás, Unaí, Gonçalves, Denise U., da Costa Rocha, Manoel O., Coelho, Eduardo A.F., and Chaves, Ana T.

Published
2025
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4. Urine and serum-based ELISA using a recombinant protein and synthetic peptide for the diagnosis of tegumentary leishmaniasis

Author

Freitas, Camila S., Câmara, Raquel S.B., Lage, Daniela P., Vale, Danniele L., Silva, Ana L., Pimenta, Breno L., Ludolf, Fernanda, Galvani, Nathália C., de Jesus, Marcelo M., Assis, Bárbara P.N., Chaves, Ana T., Tavares, Grasiele S.V., Tupinambás, Unaí, Chávez-Fumagalli, Miguel A., Pascoal, Vanessa P.M., Eller, Marcela T.C., Rocha, Manoel O. da Costa, Christodoulides, Myron, Machado-de-Ávila, Ricardo A., Gonçalves, Denise U., Pereira, Isabela A.G., and Coelho, Eduardo A.F.

Published
2025
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5. Immunization with recombinant LiHyp1 protein plus adjuvant is protective against tegumentary leishmaniasis

Author

Jesus, Marcelo M., Lage, Daniela P., Vale, Danniele L., Freitas, Camila S., Pimenta, Breno L., Moreira, Gabriel J.L., Ramos, Fernanda F., Pereira, Isabela A.G., Bandeira, Raquel S., Ludolf, Fernanda, Tavares, Grasiele S.V., Galdino, Alexsandro S., Duarte, Mariana C., Menezes-Souza, Daniel, Chávez-Fumagalli, Miguel A., Teixeira, Antônio L., Gonçalves, Denise U., Roatt, Bruno M., Christodoulides, Myron, Martins, Vívian T., and Coelho, Eduardo A.F.

Published
2023
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6. Comparison of urine and serum IgG detection ELISA for tegumentary leishmaniasis diagnosis and prognosis

Author

Câmara, Raquel S.B., Pereira, Isabela A.G., Lage, Daniela P., Vale, Danniele L., Ludolf, Fernanda, Galvani, Nathália C., Freitas, Camila S., Oliveira-da-Silva, João A., Assis, Bárbara P.N., Chaves, Ana T., Giusta, Mário S., Tavares, Grasiele S.V., Pereira, César N., Galdino, Alexsandro S., Tupinambás, Unaí, Chávez-Fumagalli, Miguel A., Pascoal, Vanessa P.M., Eller, Marcela T.C., da Costa Rocha, Manoel O., Christodoulides, Myron, Machado-de-Ávila, Ricardo A., Gonçalves, Denise U., and Coelho, Eduardo A.F.

Published
2024
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7. Non-invasive urine-based ELISA using a recombinant Leishmania protein to diagnose tegumentary leishmaniasis

Author

Câmara, Raquel S.B., Pereira, Isabela A.G., Lage, Daniela P., Vale, Danniele L., Ludolf, Fernanda, Cardoso, Mariana M, Freitas, Camila S., Oliveira-da-Silva, João A., Assis, Bárbara P.N., Chaves, Ana T., Pimenta, Breno L., Silva, Marcela G.P., Tavares, Grasiele S.V., Galdino, Alexsandro S., Tupinambás, Unaí, Chávez-Fumagalli, Miguel A., Pascoal, Vanessa P.M., Eller, Marcela T.C., Rocha, Manoel O. da Costa, Machado-de-Ávila, Ricardo A., Gonçalves, Denise U., and Coelho, Eduardo A.F.

Published
2024
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8. New synthetic molecules incorporated into polymeric micelles used for treatment against visceral leishmaniasis

Author

Freitas, Camila S., Pereira, Isabela A.G., Lage, Daniela P., Vale, Danniele L., Pimenta, Breno L., Soares, Nícia P., Santiago, Samira S., Martins, Vívian T., Câmara, Raquel S.B., Jesus, Marcelo M., Tavares, Grasiele S.V., Ramos, Fernanda F., Ludolf, Fernanda, Magalhães, Lícia N.D., Oliveira, Fabrício M., Duarte, Mariana C., Chávez-Fumagalli, Miguel A., Costa, Adilson V., Roatt, Bruno M., Teixeira, Róbson R., and Coelho, Eduardo A.F.

Published
2024
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9. Treatment using vanillin-derived synthetic molecules incorporated into polymeric micelles is effective against infection caused by Leishmania amazonensis species

Author

Pereira, Isabela A.G., Freitas, Camila S., Câmara, Raquel S.B., Jesus, Marcelo M., Lage, Daniela P., Tavares, Grasiele S.V., Soyer, Tauane G., Ramos, Fernanda F., Soares, Nícia P., Santiago, Samira S., Martins, Vívian T., Vale, Danniele L., Pimenta, Breno L., Ludolf, Fernanda, Oliveira, Fabrício M., Duarte, Mariana C., Chávez-Fumagalli, Miguel A., Costa, Adilson V., Gonçalves, Denise U., Roatt, Bruno M., Teixeira, Róbson R., and Coelho, Eduardo A.F.

Published
2024
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10. A urine-based ELISA with recombinant non-glycosylated SARS-CoV-2 spike protein for detecting anti-SARS-CoV-2 spike antibodies

Author

Ramos, Fernanda F., Bagno, Flávia F., Vassallo, Paula F., Oliveira-da-Silva, João A., Reis, Thiago A. R., Bandeira, Raquel S., Machado, Amanda S., Lage, Daniela P., Martins, Vivian T., Fernandes, Ana P., Christodoulides, Myron, Ravetti, Cecilia G., Nobre, Vandack, da Fonseca, Flávio G., Coelho, Eduardo A. F., and Ludolf, Fernanda

Published
2023
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11. The association between rLiHyp1 protein plus adjuvant and amphotericin B is an effective immunotherapy against visceral leishmaniasis in mice

Author

Lage, Daniela P., Martins, Vívian T., Vale, Danniele L., Freitas, Camila S., Pimenta, Breno L., Moreira, Gabriel J.L., Ramos, Fernanda F., Pereira, Isabela A.G., Bandeira, Raquel S., de Jesus, Marcelo M., Ludolf, Fernanda, Tavares, Grasiele S.V., Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Christodoulides, Myron, and Coelho, Eduardo A.F.

Published
2023
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12. In vitro evaluation of antileishmanial activity, mode of action and cellular response induced by vanillin synthetic derivatives against Leishmania species able to cause cutaneous and visceral leishmaniasis

Author

Freitas, Camila S., Santiago, Samira S., Lage, Daniela P., Antinarelli, Luciana M.R., Oliveira, Fabrício M., Vale, Danniele L., Martins, Vívian T., Magalhaes, Lícia N.D., Bandeira, Raquel S., Ramos, Fernanda F., Pereira, Isabela A.G., de Jesus, Marcelo M., Ludolf, Fernanda, Tavares, Grasiele S.V., Costa, Adilson V., Ferreira, Rafaela S., Coimbra, Elaine S., Teixeira, Róbson R., and Coelho, Eduardo A.F.

Published
2023
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13. Exploring drug repositioning for leishmaniasis treatment: Ivermectin plus polymeric micelles induce immunological response and protection against tegumentary leishmaniasis

Author

Freitas, Camila S., Lage, Daniela P., Machado, Amanda S., Vale, Danniele L., Martins, Vívian T., Cardoso, Jamille M.O., Oliveira-da-Silva, João A., Reis, Thiago A.R., Tavares, Grasiele S.V., Ramos, Fernanda F., Ludolf, Fernanda, Pereira, Isabela A.G., Bandeira, Raquel S., Fujiwara, Ricardo T., Bueno, Lílian L., Roatt, Bruno M., Chávez-Fumagalli, Miguel A., and Coelho, Eduardo A.F.

Published
2023
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14. Evaluation from a B-cell epitope-based chimeric protein for the serodiagnosis of tegumentary and visceral leishmaniasis

Author

Vale, Danniele L., Machado, Amanda S., Ramos, Fernanda F., Lage, Daniela P., Freitas, Camila S., de Oliveira, Daysiane, Galvani, Nathalia C., Luiz, Gabriel P., Fagundes, Mirian I., Fernandes, Bruna B., Oliveira-da-Silva, João A., Ludolf, Fernanda, Tavares, Grasiele S.V., Guimarães, Nathalia S., Chaves, Ana T., Chávez-Fumagalli, Miguel A., Tupinambás, Unaí, Rocha, Manoel O.C., Gonçalves, Denise U., Martins, Vívian T., Machado-de-Ávila, Ricardo A., and Coelho, Eduardo A.F.

Published
2022
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15. A recombinant Leishmania amastigote-specific protein, rLiHyG, with adjuvants, protects against infection with Leishmania infantum

Author

Machado, Amanda S., Lage, Daniela P., Vale, Danniele L., Freitas, Camila S., Linhares, Flávia P., Cardoso, Jamille M.O., Pereira, Isabela A.G., Ramos, Fernanda F., Tavares, Grasiele S.V., Ludolf, Fernanda, Oliveira-da-Silva, João A., Bandeira, Raquel S., Simões, Aratti C., Duarte, Mariana C., Oliveira, Jamil S., Christodoulides, Myron, Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Martins, Vívian T., and Coelho, Eduardo A.F.

Published
2022
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16. Recombinant guanosine-5′-triphosphate (GTP)-binding protein associated with Poloxamer 407-based polymeric micelles protects against Leishmania infantum infection

Author

Lage, Daniela P., Machado, Amanda S., Vale, Danniele L., Freitas, Camila S., Linhares, Flávia P., Cardoso, Jamille M.O., Pereira, Isabela A.G., Ramos, Fernanda F., Tavares, Grasiele S.V., Ludolf, Fernanda, Oliveira-da-Silva, João A., Bandeira, Raquel S., Silva, Alessandra M., Simões, Luciana C., Reis, Thiago A.R., Oliveira, Jamil S., Christodoulides, Myron, Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Martins, Vívian T., and Coelho, Eduardo A.F.

Published
2022
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17. Flau-A, a naphthoquinone derivative, is a promising therapeutic candidate against visceral leishmaniasis: A preliminary study

Author

Mendonça, Débora V.C., Tavares, Grasiele S.V., Pereira, Isabela A.G., Oliveira-da-Silva, João A., Ramos, Fernanda F., Lage, Daniela P., Machado, Amanda S., Carvalho, Lívia M., Reis, Thiago A.R., Carvalho, Ana Maria R.S., Ottoni, Flaviano M., Ludolf, Fernanda, Freitas, Camila S., Martins, Vívian T., Chávez-Fumagalli, Miguel A., Duarte, Mariana C., Humbert, Maria V., Roatt, Bruno M., Menezes-Souza, Daniel, Alves, Ricardo J., and Coelho, Eduardo A.F.

Published
2022
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18. Sensitive and specific serodiagnosis of tegumentary leishmaniasis using a new chimeric protein based on specific B-cell epitopes of Leishmania antigenic proteins

Author

Galvani, Nathalia C., Machado, Amanda S., Lage, Daniela P., Martins, Vívian T., de Oliveira, Daysiane, Freitas, Camila S., Vale, Danniele L., Fernandes, Bruna B., Oliveira-da-Silva, João A., Reis, Thiago A.R., Santos, Thaís T.O., Ramos, Fernanda F., Bandeira, Raquel S., Ludolf, Fernanda, Tavares, Grasiele S.V., Guimarães, Nathalia S., Tupinambás, Unaí, Chávez-Fumagalli, Miguel A., Humbert, Maria V., Gonçalves, Denise U., Christodoulides, Myron, Machado-de-Ávila, Ricardo A., and Coelho, Eduardo A.F.

Published
2022
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19. Potential of recombinant LiHyQ, a novel Leishmania infantum protein, for the diagnosis of canine visceral leishmaniasis and as a diagnostic and prognostic marker for human leishmaniasis and human immunodeficiency virus co-infection: A preliminary study

Author

Santos, Thaís T.O., Ramos, Fernanda F., Gonçalves, Isabela A.P., Tavares, Grasiele S.V., Ludolf, Fernanda, Bandeira, Raquel S., Silva, Alessandra M., Oliveira-da-Silva, João A., Reis, Thiago A.R., Machado, Amanda S., Lage, Daniela P., Freitas, Camila S., Vale, Danniele L., Martins, Vívian T., Alves, Livia A., Guimarães, Nathalia S., Chaves, Ana Thereza, Chávez-Fumagalli, Miguel A., Cota, Gláucia F., Silveira, Julia A.G., Tupinambás, Unaí, Gonçalves, Denise U., Christodoulides, Myron, and Coelho, Eduardo A.F.

Published
2021
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20. ChimLeish, a new recombinant chimeric protein evaluated as a diagnostic and prognostic marker for visceral leishmaniasis and human immunodeficiency virus coinfection

Author

Galvani, Nathalia C., Machado, Amanda S., Lage, Daniela P., Freitas, Camila S., Vale, Danniele L., de Oliveira, Daysiane, Ludolf, Fernanda, Ramos, Fernanda F., Fernandes, Bruna B., Luiz, Gabriel P., Mendonça, Débora V. C., Oliveira-da-Silva, João A., Reis, Thiago A. R., Tavares, Grasiele S. V., Chaves, Ana T., Guimarães, Nathalia S., Tupinambás, Unaí, Cota, Gláucia F., Humbert, Maria V., Martins, Vívian T., Christodoulides, Myron, Coelho, Eduardo A. F., and Machado-de-Ávila, Ricardo A.

Published
2021
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21. Leishmania eukaryotic elongation Factor-1 beta protein is immunogenic and induces parasitological protection in mice against Leishmania infantum infection

Author

Santos, Thaís T.O., Machado, Amanda S., Ramos, Fernanda F., Oliveira-da-Silva, João A., Lage, Daniela P., Tavares, Grasiele S.V., Mendonça, Débora V.C., Cardoso, Mariana S., Siqueira, Williane F., Martins, Vívian T., Ludolf, Fernanda, Reis, Thiago A.R., Carvalho, Lívia M., Freitas, Camila S., Bandeira, Raquel S., Silva, Alessandra M., Oliveira, Jamil S., Moreira, Ricardo L.F., Fujiwara, Ricardo T., Roatt, Bruno M., Chávez-Fumagalli, Miguel A., Humbert, Maria V., Teixeira, Antônio L., and Coelho, Eduardo A.F.

Published
2021
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22. Ivermectin presents effective and selective antileishmanial activity in vitro and in vivo against Leishmania infantum and is therapeutic against visceral leishmaniasis

Author

Reis, Thiago A.R., Oliveira-da-Silva, João A., Tavares, Grasiele S.V., Mendonça, Débora V.C., Freitas, Camila S., Costa, Rafaella R., Lage, Daniela P., Martins, Vívian T., Machado, Amanda S., Ramos, Fernanda F., Silva, Alessandra M., Ludolf, Fernanda, Antinarelli, Luciana M.R., Brito, Rory C.F., Chávez-Fumagalli, Miguel A., Humbert, Maria V., Roatt, Bruno M., Coimbra, Elaine S., and Coelho, Eduardo A.F.

Published
2021
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23. Acarbose presents in vitro and in vivo antileishmanial activity against Leishmania infantum and is a promising therapeutic candidate against visceral leishmaniasis

Author

Costa, Rafaella R., Oliveira-da-Silva, João A., Reis, Thiago A. R., Tavares, Grasiele S. V., Mendonça, Débora V. C., Freitas, Camila S., Lage, Daniela P., Martins, Vívian T., Antinarelli, Luciana M. R., Machado, Amanda S., Bandeira, Raquel S., Ludolf, Fernanda, Santos, Thaís T. O., Brito, Rory C. F., Humbert, Maria V., Menezes-Souza, Daniel, Duarte, Mariana C., Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Coimbra, Elaine S., and Coelho, Eduardo A. F.

Published
2021
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24. Biotechnological applications from a Leishmania amastigote-specific hypothetical protein in the canine and human visceral leishmaniasis

Author

Oliveira-da-Silva, João A., Machado, Amanda S., Tavares, Grasiele S.V., Ramos, Fernanda F., Lage, Daniela P., Ludolf, Fernanda, Steiner, Bethina T., Reis, Thiago A.R., Santos, Thaís T.O., Costa, Lourena E., Bandeira, Raquel S., Martins, Vívian T., Galvani, Nathália C., Chaves, Ana T., Oliveira, Jamil S., Chávez-Fumagalli, Miguel A., Tupinambás, Unaí, de Magalhães-Soares, Danielle F., Silveira, Julia A.G., Lyon, Sandra, Machado-de-Ávila, Ricardo A., and Coelho, Eduardo A.F.

Published
2020
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25. A Leishmania amastigote-specific hypothetical protein evaluated as recombinant protein plus Th1 adjuvant or DNA plasmid-based vaccine to protect against visceral leishmaniasis

Author

Oliveira-da-Silva, João A., Machado, Amanda S., Ramos, Fernanda F., Tavares, Grasiele S.V., Lage, Daniela P., Mendonça, Débora V.C., Pereira, Isabela A.G., Santos, Thaís T.O., Martins, Vívian T., Carvalho, Lívia M., Freitas, Camila S., Ludolf, Fernanda, Reis, Thiago A.R., Bandeira, Raquel S., Silva, Alessandra M., Costa, Lourena E., Oliveira, Jamil S., Duarte, Mariana C., Roatt, Bruno M., Teixeira, Antônio L., and Coelho, Eduardo A.F.

Published
2020
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26. A new Leishmania hypothetical protein can be used for accurate serodiagnosis of canine and human visceral leishmaniasis and as a potential prognostic marker for human disease

Author

Machado, Amanda S., Ramos, Fernanda F., Santos, Thaís T.O., Costa, Lourena E., Ludolf, Fernanda, Lage, Daniela P., Bandeira, Raquel S., Tavares, Grasiele S.V., Oliveira-da-Silva, João A., Steiner, Bethina T., Chaves, Ana T., Oliveira, Jamil S., Chávez-Fumagalli, Miguel A., de Magalhães-Soares, Danielle F., Silveira, Julia A.G., Duarte, Mariana C., Machado-de-Ávila, Ricardo A., Lyon, Sandra, Gonçalves, Denise U., Caligiorne, Rachel B., and Coelho, Eduardo A.F.

Published
2020
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27. Leishmania infantum pyridoxal kinase evaluated in a recombinant protein and DNA vaccine to protects against visceral leishmaniasis

Author

Oliveira-da-Silva, João A., Lage, Daniela P., Ramos, Fernanda F., Machado, Amanda S., Tavares, Grasiele S.V., Mendonça, Débora V.C., Pereira, Isabela A.G., Martins, Vívian T., Carvalho, Lívia M., Ludolf, Fernanda, Santos, Thaís T.O., Reis, Thiago A.R., Oliveira, Camila S., Bandeira, Raquel S., Silva, Alessandra M., Costa, Lourena E., Oliveira, Jamil S., Duarte, Mariana C., Menezes-Souza, Daniel, Roatt, Bruno M., Teixeira, Antônio L., and Coelho, Eduardo A.F.

Published
2020
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28. Evaluation of Leishmania infantum pyridoxal kinase protein for the diagnosis of human and canine visceral leishmaniasis

Author

Oliveira-da-Silva, João A., Machado, Amanda S., Ramos, Fernanda F., Tavares, Grasiele S.V., Lage, Daniela P., Ludolf, Fernanda, Steiner, Bethina T., Reis, Thiago A.R., Santos, Thaís T.O., Costa, Lourena E., Martins, Vívian T., Galvani, Nathália C., Chaves, Ana T., Oliveira, Jamil S., Chávez-Fumagalli, Miguel A., de Magalhães-Soares, Danielle F., Duarte, Mariana C., Menezes-Souza, Daniel, Silveira, Julia A.G., Moreira, Ricardo L.F., Machado-de-Ávila, Ricardo A., Tupinambás, Unaí, Gonçalves, Denise U., and Coelho, Eduardo A.F.

Published
2020
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29. A Leishmania infantum hypothetical protein evaluated as a recombinant protein and specific B-cell epitope for the serodiagnosis and prognosis of visceral leishmaniasis

Author

Machado, Amanda S., Ramos, Fernanda F., Oliveira-da-Silva, João A., Santos, Thaís T.O., Ludolf, Fernanda, Tavares, Grasiele S.V., Costa, Lourena E., Lage, Daniela P., Steiner, Bethina T., Chaves, Ana T., Chávez-Fumagalli, Miguel A., de Magalhães-Soares, Danielle F., Silveira, Julia A.G., Napoles, Karina M.N., Tupinambás, Unaí, Duarte, Mariana C., Machado-de-Ávila, Ricardo A., Bueno, Lílian L., Fujiwara, Ricardo T., Moreira, Ricardo L.F., Rocha, Manoel O.C., Caligiorne, Rachel B., and Coelho, Eduardo A.F.

Published
2020
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30. Digitoxigenin presents an effective and selective antileishmanial action against Leishmania infantum and is a potential therapeutic agent for visceral leishmaniasis

Author

Freitas, Camila S., Oliveira-da-Silva, João A., Lage, Daniela P., Costa, Rafaella R., Mendonça, Débora V. C., Martins, Vívian T., Reis, Thiago A. R., Antinarelli, Luciana M. R., Machado, Amanda S., Tavares, Grasiele S. V., Ramos, Fernanda F., Coelho, Vinicio T. S., Brito, Rory C. F., Ludolf, Fernanda, Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Ramos, Gabriela S., Munkert, Jennifer, Ottoni, Flaviano M., Campana, Priscilla R. V., Humbert, Maria V., Coimbra, Elaine S., Braga, Fernão C., Pádua, Rodrigo M., and Coelho, Eduardo A. F.

Published
2021
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31. A chloroquinoline derivate presents effective in vitro and in vivo antileishmanial activity against Leishmania species that cause tegumentary and visceral leishmaniasis

Author

Sousa, Jessica K.T., Antinarelli, Luciana M.R., Mendonça, Débora V.C., Lage, Daniela P., Tavares, Grasiele S.V., Dias, Daniel S., Ribeiro, Patrícia A.F., Ludolf, Fernanda, Coelho, Vinicio T.S., Oliveira-da-Silva, João A., Perin, Luísa, Oliveira, Bianka A., Alvarenga, Denis F., Chávez-Fumagalli, Miguel A., Brandão, Geraldo C., Nobre, Vandack, Pereira, Guilherme R., Coimbra, Elaine S., and Coelho, Eduardo A.F.

Published
2019
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32. Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope

Author

Rodrigues, Marcella R., Santos, Lucas M.O., Miyazaki, Carolina K., Martins, Vivian T., Ludolf, Fernanda R., Kursancew, Amanda C., Ramos, Fernanda F., Dias, Daniel S., Oliveira, Jamil S., Vieira, Paula M.A., Roatt, Bruno M., Machado de Ávila, Ricardo A., Gonçalves, Denise U., Menezes-Souza, Daniel, Coelho, Eduardo A.F., and Duarte, Mariana C.

Published
2019
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33. Evaluation of the in vitro and in vivo antileishmanial activity of a chloroquinolin derivative against Leishmania species capable of causing tegumentary and visceral leishmaniasis

Author

Soyer, Tauane G., Mendonça, Débora V.C., Tavares, Grasiele S.V., Lage, Daniela P., Dias, Daniel S., Ribeiro, Patrícia A.F., Perin, Luisa, Ludolf, Fernanda, Coelho, Vinicio T.S., Ferreira, Andreza C.G., Neves, Pedro H.A.S., Matos, Guilherme F., Chávez-Fumagalli, Miguel A., Coimbra, Elaine S., Pereira, Guilherme R., Coelho, Eduardo A.F., and Antinarelli, Luciana M.R.

Published
2019
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35. A Pluronic® F127-based polymeric micelle system containing an antileishmanial molecule is immunotherapeutic and effective in the treatment against Leishmania amazonensis infection

Author

Tavares, Grasiele S.V., Mendonça, Débora V.C., Miyazaki, Carolina K., Lage, Daniela P., Soyer, Tauane G., Carvalho, Lívia M., Ottoni, Flaviano M., Dias, Daniel S., Ribeiro, Patrícia A.F., Antinarelli, Luciana M.R., Ludolf, Fernanda, Duarte, Mariana C., Coimbra, Elaine S., Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Menezes-Souza, Daniel, Barichello, José Mário, Alves, Ricardo J., and Coelho, Eduardo A.F.

Published
2019
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36. Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection

Author

Ribeiro, Patrícia A.F., Dias, Daniel S., Lage, Daniela P., Martins, Vívian T., Costa, Lourena E., Santos, Thaís T.O., Ramos, Fernanda F., Tavares, Grasiele S.V., Mendonça, Débora V.C., Ludolf, Fernanda, Gomes, Dawidson A., Rodrigues, Michele A., Chávez-Fumagalli, Miguel A., Silva, Eduardo S., Galdino, Alexsandro S., Duarte, Mariana C., Roatt, Bruno M., Menezes-Souza, Daniel, Teixeira, Antonio L., and Coelho, Eduardo A.F.

Published
2019
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37. In vivo antileishmanial efficacy of a naphthoquinone derivate incorporated into a Pluronic® F127-based polymeric micelle system against Leishmania amazonensis infection

Author

Mendonça, Débora V.C., Tavares, Grasiele S.V., Lage, Daniela P., Soyer, Tauane G., Carvalho, Lívia M., Dias, Daniel S., Ribeiro, Patrícia A.F., Ottoni, Flaviano M., Antinarelli, Luciana M.R., Vale, Danniele L., Ludolf, Fernanda, Duarte, Mariana C., Coimbra, Elaine S., Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Menezes-Souza, Daniel, Barichello, José Mário, Alves, Ricardo J., and Coelho, Eduardo A.F.

Published
2019
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38. A Leishmania hypothetical protein-containing liposome-based formulation is highly immunogenic and induces protection against visceral leishmaniasis

Author

Ribeiro, Patrícia A.F., Dias, Daniel S., Novais, Marcus V.M., Lage, Daniela P., Tavares, Grasiele S.V., Mendonça, Débora V.C., Oliveira, Jamil S., Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Duarte, Mariana C., Menezes-Souza, Daniel, Ludolf, Fernanda, Tavares, Carlos A.P., Oliveira, Mônica C., and Coelho, Eduardo A.F.

Published
2018
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39. Vaccination with a CD4+ and CD8+ T-cell epitopes-based recombinant chimeric protein derived from Leishmania infantum proteins confers protective immunity against visceral leishmaniasis

Author

Dias, Daniel S., Ribeiro, Patrícia A.F., Martins, Vívian T., Lage, Daniela P., Costa, Lourena E., Chávez-Fumagalli, Miguel A., Ramos, Fernanda F., Santos, Thaís T.O., Ludolf, Fernanda, Oliveira, Jamil S., Mendes, Tiago A.O., Silva, Eduardo S., Galdino, Alexsandro S., Duarte, Mariana C., Roatt, Bruno M., Menezes-Souza, Daniel, Teixeira, Antonio L., and Coelho, Eduardo A.F.

Published
2018
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40. Immunotherapy Combining Mimotopes Selected by Phage Display Plus Amphotericin B Is Effective for Treatment Against Visceral Leishmaniasis.

Author

Soyer, Tauane Gonçalves, Bandeira Câmara, Raquel Soares, Pereira, Isabela Amorim Gonçalves, Ramos, Fernanda Fonseca, de Jesus, Marcelo Moreira, Ludolf, Fernanda, de Paula Costa, Guilherme, Lage, Daniela Pagliara, de Freitas, Camila Simões, Vale, Danniele Luciana, Pimenta, Breno Luiz, Martins, Vívian Tamietti, Galdino, Alexsandro Sobreira, Chávez‐Fumagalli, Miguel Angel, Roatt, Bruno Mendes, de Sousa Vieira Tavares, Grasiele, and Coelho, Eduardo Antonio Ferraz

Subjects
VISCERAL leishmaniasis, AMPHOTERICIN B, BACTERIOPHAGES, LEISHMANIA donovani, IMMUNOTHERAPY, LEISHMANIA infantum, LEISHMANIA
Abstract

The treatment for visceral leishmaniasis (VL) causes toxicity in patients, entails high cost and/or leads to the emergence of resistant strains. No human vaccine exists, and diagnosis presents problems related to the sensitivity or specificity of the tests. Here, we tested two phage clones, B1 and D11, which were shown to be protective against Leishmania infantum infection in a murine model as immunotherapeutics to treat mice infected with this parasite species. The phages were used alone or with amphotericin B (AmpB), while other mice received saline, AmpB, a wild‐type phage (WTP) or WTP/AmpB. Results showed that the B1/AmpB and D11/AmpB combinations induced polarised Th1‐type cellular and humoral responses, which were primed by high levels of parasite‐specific IFN‐γ, IL‐12, TNF‐α, nitrite and IgG2a antibodies, which reflected in significant reductions in the parasite load in distinct organs of the animals when analyses were performed 1 and 30 days after the treatments. Reduced organic toxicity was also found in these animals, as compared with the controls. In conclusion, preliminary data suggest the potential of the B1/AmpB and D11/AmpB combinations as immunotherapeutics against L. infantum infection. [ABSTRACT FROM AUTHOR]

Published
2024
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41. A vaccine composed of a hypothetical protein and the eukaryotic initiation factor 5a from Leishmania braziliensis cross-protection against Leishmania amazonensis infection

Author

Duarte, Mariana Costa, Lage, Daniela Pagliara, Martins, Vívian Tamietti, Costa, Lourena Emanuele, Carvalho, Ana Maria Ravena Severino, Ludolf, Fernanda, Santos, Thaís Teodoro de Oliveira, Vale, Danniele Luciana, Roatt, Bruno Mendes, Menezes-Souza, Daniel, Fernandes, Ana Paula, Tavares, Carlos Alberto Pereira, and Coelho, Eduardo Antonio Ferraz

Published
2017
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42. Poloxamer 407 (Pluronic® F127)-based polymeric micelles for amphotericin B: In vitro biological activity, toxicity and in vivo therapeutic efficacy against murine tegumentary leishmaniasis

Author

Mendonça, Débora V.C., Lage, Letícia M.R., Lage, Daniela P., Chávez-Fumagalli, Miguel A., Ludolf, Fernanda, Roatt, Bruno M., Menezes-Souza, Daniel, Faraco, André A.G., Castilho, Rachel O., Tavares, Carlos A.P., Barichello, José Mário, Duarte, Mariana C., and Coelho, Eduardo A.F.

Published
2016
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44. Antileishmanial activity of a naphthoquinone derivate against promastigote and amastigote stages of Leishmania infantum and Leishmania amazonensis and its mechanism of action against L. amazonensis species

Author

Mendonça, Débora Vasconcelos Costa, Lage, Daniela Pagliara, Calixto, Stephane Lima, Ottoni, Flaviano Melo, Tavares, Grasiele de Sousa Vieira, Ludolf, Fernanda, Chávez-Fumagalli, Miguel Angel, Schneider, Mônica Santos, Duarte, Mariana Costa, Tavares, Carlos Alberto Pereira, Alves, Ricardo José, Coimbra, Elaine Soares, and Coelho, Eduardo Antonio Ferraz

Published
2017
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45. Efficacy of an Immunotherapy Combining Immunogenic Chimeric Protein Plus Adjuvant and Amphotericin B against Murine Visceral Leishmaniasis.

Author

Vale, Danniele L., Freitas, Camila S., Martins, Vívian T., Moreira, Gabriel J. L., Machado, Amanda S., Ramos, Fernanda F., Pereira, Isabela A. G., Bandeira, Raquel S., de Jesus, Marcelo M., Tavares, Grasiele S. V., Ludolf, Fernanda, Chávez-Fumagalli, Miguel A., Galdino, Alexsandro S., Fujiwara, Ricardo T., Bueno, Lílian L., Roatt, Bruno M., Christodoulides, Myron, Coelho, Eduardo A. F., and Lage, Daniela P.

Subjects
VISCERAL leishmaniasis, AMPHOTERICIN B, CHIMERIC proteins, NEGLECTED diseases, IMMUNOGLOBULINS, LEUKOCYTES, T cells
Abstract

Simple Summary: Visceral leishmaniasis is a tropical neglected disease caused by infection with Leishmania parasites. There are no human vaccines and the current drug treatments for infected patients are hampered by toxicity and the development of resistance. In our study, we have used an immunotherapeutic approach, which combines a vaccine and drug, to treat mice infected with the parasite. This involved injecting a candidate Leishmania vaccine ChimT with the adjuvant monophosphoryl lipid A (MPLA, which enhances the immune response) and a treatment drug amphotericin B (AmpB). We found that the combined immunotherapy of ChimT/MPLA/AmpB significantly reduced the number of parasites within infected internal organs of the animals and increased the production of protective antibodies. Organ toxicity was also lower with the ChimT/MPLA/AmpB immunotherapy, suggesting that the inclusion of the vaccine and adjuvant reduced the inherent toxicity of the drug. In addition, the ChimT vaccine stimulated in vitro mouse white cells to kill three different Leishmania parasites that had invaded the cells. We conclude that the combination of ChimT/MPLA/AmpB could be considered for further studies as an immunotherapy for Leishmania infection. Visceral leishmaniasis (VL) in the Americas is a chronic systemic disease caused by infection with Leishmania infantum parasites. The toxicity of antileishmanial drugs, long treatment course and limited efficacy are significant concerns that hamper adequate treatment against the disease. Studies have shown the promise of an immunotherapeutics approach, combining antileishmanial drugs to reduce the parasitism and vaccine immunogens to activate the host immune system. In the current study, we developed an immunotherapy using a recombinant T cell epitope-based chimeric protein, ChimT, previously shown to be protective against Leishmania infantum, with the adjuvant monophosphoryl lipid A (MPLA) and amphotericin B (AmpB) as the antileishmanial drug. BALB/c mice were infected with L. infantum stationary promastigotes and later they received saline or were treated with AmpB, MPLA, ChimT/Amp, ChimT/MPLA or ChimT/MPLA/AmpB. The combination of ChimT/MPLA/AmpB significantly reduced the parasite load in mouse organs (p < 0.05) and induced a Th1-type immune response, which was characterized by higher ratios of anti-ChimT and anti-parasite IgG2a:IgG1 antibodies, increased IFN-γ mRNA and IFN-γ and IL-12 cytokines and accompanied by lower levels of IL-4 and IL-10 cytokines, when compared to other treatments and controls (all p < 0.05). Organ toxicity was also lower with the ChimT/MPLA/AmpB immunotherapy, suggesting that the inclusion of the vaccine and adjuvant ameliorated the toxicity of AmpB to some degree. In addition, the ChimT vaccine alone stimulated in vitro murine macrophages to significantly kill three different internalized species of Leishmania parasites and to produce Th1-type cytokines into the culture supernatants. To conclude, our data suggest that the combination of ChimT/MPLA/AmpB could be considered for further studies as an immunotherapy for L. infantum infection. [ABSTRACT FROM AUTHOR]

Published
2023
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48. Leishmania LiHyC protein is immunogenic and induces protection against visceral leishmaniasis.

Author

Machado, Amanda S., Lage, Daniela P., Vale, Danniele L., Freitas, Camila S., Linhares, Flávia P., Cardoso, Jamille M. O., Oliveira‐da‐Silva, João A., Pereira, Isabela A. G., Ramos, Fernanda F., Tavares, Grasiele S. V., Ludolf, Fernanda, Bandeira, Raquel S., Maia, Luiz G. N., Menezes‐Souza, Daniel, Duarte, Mariana C., Chávez‐Fumagalli, Miguel A., Roatt, Bruno M., Christodoulides, Myron, Martins, Vívian T., and Coelho, Eduardo Antonio Ferraz

Subjects
VISCERAL leishmaniasis, MACROPHAGE colony-stimulating factor, LEISHMANIASIS, GRANULOCYTE-macrophage colony-stimulating factor, RECOMBINANT proteins, LEISHMANIA, LEISHMANIA infantum
Abstract

Treatment against visceral leishmaniasis (VL) presents problems by the toxicity of drugs, high cost and/or emergence of resistant strains. The diagnosis is hampered by variable sensitivity and/or specificity of tests. In this context, prophylactic vaccination could represent a control measure against disease. In this study, the protective efficacy of Leishmania LiHyC protein was evaluated in a murine model against Leishmania infantum infection. LiHyC was used as recombinant protein (rLiHyC) associated with saponin (rLiHyC/S) or Poloxamer 407‐based polymeric micelles (rLiHyC/M) to immunize mice. Animals received also saline, saponin or empty micelles as controls. The immunogenicity was evaluated before and after the challenge, and results showed that vaccination with rLiHyC/S or rLiHyC/M induced the production of high levels of interferon‐gamma (IFN‐γ), interleukin (IL)‐12 and granulocyte‐macrophage colony‐stimulating factor in cell culture supernatants, as well as higher IFN‐γ expression evaluated by RT‐qPCR and involvement from CD4+ and CD8+ T‐cell subtypes producing IFN‐γ, tumor necrosis factor‐α and IL‐2. A positive lymphoproliferative response was also found in cell cultures from vaccinated animals, besides high levels of rLiHyC‐ and parasite‐specific nitrite and IgG2a antibodies. Immunological assays correlated with significant reductions in the parasite load in the spleens, livers, bone marrows and draining lymph nodes from vaccinated mice, when compared to values found in the controls. The micellar composition showed slightly better immunological and parasitological data, as compared to rLiHyC/S. Results suggest that rLiHyC associated with adjuvants could be considered for future studies as a vaccine candidate against VL. [ABSTRACT FROM AUTHOR]

Published
2022
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49. A Recombinant Chimeric Protein-Based Vaccine Containing T-Cell Epitopes from Amastigote Proteins and Combined with Distinct Adjuvants, Induces Immunogenicity and Protection against Leishmania infantum Infection.

Author

Lage, Daniela P., Vale, Danniele L., Linhares, Flávia P., Freitas, Camila S., Machado, Amanda S., Cardoso, Jamille M. O., de Oliveira, Daysiane, Galvani, Nathália C., de Oliveira, Marcelo P., Oliveira-da-Silva, João A., Ramos, Fernanda F., Tavares, Grasiele S. V., Ludolf, Fernanda, Bandeira, Raquel S., Pereira, Isabela A. G., Chávez-Fumagalli, Miguel A., Roatt, Bruno M., Machado-de-Ávila, Ricardo A., Christodoulides, Myron, and Coelho, Eduardo A. F.

Subjects
LEISHMANIA infantum, T cells, CHIMERIC proteins, IMMUNE response, EPITOPES
Abstract

Currently, there is no licensed vaccine to protect against human visceral leishmaniasis (VL), a potentially fatal disease caused by infection with Leishmania parasites. In the current study, a recombinant chimeric protein ChimT was developed based on T-cell epitopes identified from the immunogenic Leishmania amastigote proteins LiHyp1, LiHyV, LiHyC and LiHyG. ChimT was associated with the adjuvants saponin (Sap) or monophosphoryl lipid A (MPLA) and used to immunize mice, and their immunogenicity and protective efficacy were evaluated. Both ChimT/Sap and ChimT/MPLA induced the development of a specific Th1-type immune response, with significantly high levels of IFN-γ, IL-2, IL-12, TNF-α and GM-CSF cytokines produced by CD4+ and CD8+ T cell subtypes (p < 0.05), with correspondingly low production of anti-leishmanial IL-4 and IL-10 cytokines. Significantly increased (p < 0.05) levels of nitrite, a proxy for nitric oxide, and IFN-γ expression (p < 0.05) were detected in stimulated spleen cell cultures from immunized and infected mice, as was significant production of parasite-specific IgG2a isotype antibodies. Significant reductions in the parasite load in the internal organs of the immunized and infected mice (p < 0.05) were quantified with a limiting dilution technique and quantitative PCR and correlated with the immunological findings. ChimT/MPLA showed marginally superior immunogenicity than ChimT/Sap, and although this was not statistically significant (p > 0.05), ChimT/MPLA was preferred since ChimT/Sap induced transient edema in the inoculation site. ChimT also induced high IFN-γ and low IL-10 levels from human PBMCs isolated from healthy individuals and from VL-treated patients. In conclusion, the experimental T-cell multi-epitope amastigote stage Leishmania vaccine administered with adjuvants appears to be a promising vaccine candidate to protect against VL. [ABSTRACT FROM AUTHOR]

Published
2022
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50. A clioquinol-containing Pluronic® F127 polymeric micelle system is effective in the treatment of visceral leishmaniasis in a murine model.

Author

Tavares, Grasiele S.V., Mendonça, Débora V.C., Pereira, Isabela A.G., Oliveira-da-Silva, João A., Ramos, Fernanda F., Lage, Daniela P., Machado, Amanda S., Carvalho, Lívia M., Reis, Thiago A.R., Perin, Luísa, Carvalho, Ana Maria R.S., Ottoni, Flaviano M., Ludolf, Fernanda, Freitas, Camila S., Bandeira, Raquel S., Silva, Alessandra M., Chávez-Fumagalli, Miguel A., Duarte, Mariana C., Menezes-Souza, Daniel, and Alves, Ricardo J.

Abstract

Copyright of Parasite (1252607X) is the property of EDP Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2020
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