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Background Autonomic failure (AF) complicates Parkinson’s disease (PD) in one-third of cases, resulting in complex blood pressure (BP) abnormalities. While autonomic testing represents the diagnostic gold standard for AF, accessibility to this examination remains limited to a few tertiary referral centers. Objective The present study sought to investigate the accuracy of a machine learning algorithm applied to 24-h ambulatory BP monitoring (ABPM) as a tool to facilitate the diagnosis of AF in patients with PD. Methods Consecutive PD patients naïve to vasoactive medications underwent 24 h-ABPM and autonomic testing. The diagnostic accuracy of a Linear Discriminant Analysis (LDA) model exploiting ABPM parameters was compared to autonomic testing (as per a modified version of the Composite Autonomic Symptom Score not including the sudomotor score) in the diagnosis of AF. Results The study population consisted of n = 80 PD patients (33% female) with a mean age of 64 ± 10 years old and disease duration of 6.2 ± 4 years. The prevalence of AF at the autonomic testing was 36%. The LDA model showed 91.3% accuracy (98.0% specificity, 79.3% sensitivity) in predicting AF, significantly higher than any of the ABPM variables considered individually (hypotensive episodes = 82%; reverse dipping = 79%; awakening hypotension = 74%). Conclusion LDA model based on 24-h ABPM parameters can effectively predict AF, allowing greater accessibility to an accurate and easy to administer test for AF. Potential applications range from systematic AF screening to monitoring and treating blood pressure dysregulation caused by PD and other neurodegenerative disorders.

In this work we present the hydrogeophysical imaging of a key sector of the Quaternary Po foreland basin (northern Italy), focussing on the reconstruction of clastic aquifers and aquitards in a complex tectono-sedimentary subsurface architecture. The study area includes the relic reliefs of Casalpusterlengo and Zorlesco, two smooth morphological features involving uplifted and gently folded Pleistocene marine to alluvial sediments, plausibly linked to the buried Northern Apennines thrust and fold belt. The geophysical data include 35 Direct Current Vertical Electrical Soundings collected over a 37 km2 wide area, acquired with Schlumberger array and maximum half-spacing of 500 m. 1-D resistivity-depth profiles were computed for each VES. An integrated hydrostratigraphic approach was applied, to constrain the interpretation of the geophysical data along several cross-sections, including the comparison of resistivity soundings to stratigraphic logs, borehole electric logs and the pore-water properties. The resistivity interfaces, traceable with the same laterally continuous vertical polarity, were used to develop an electrostratigraphic model in order to portray the stacking of electrostratigraphic units down to 200 m below ground surface. Their vertical associations show a general upward increase of electrical resistivity. This assemblage mimics the regional coarsening upwards depositional trend, from the conductive units of the Plio-Pleistocene marine-to-transitional depositional systems to the resistive units of the Middle–Late Pleistocene fluvial and alluvial plain depositional systems. Middle Pleistocene depositional systems host an alternation of North-dipping, high-to-intermediate permeability aquifer systems (70–180 Ωm, thickness of 5–70 m) separated by low permeability aquitards (20–50 Ωm, thickness up to 40 m). These units pinch out against the Casalpusterlengo and Zorlesco relic reliefs, where they cover the uplifted and folded regional aquitard (20–50 Ωm) formed by Pliocene-Lower Pleistocene clays to sandy silts with gravel lenses in agreement with borehole data. In the deepest part of the local stratigraphy, a broad low-resistivity anomaly (< 10 Ωm) was clearly mapped through the study area. By comparison with electrical borehole logs in deep oil-wells, it could be interpreted as the fresh–saltwater interface due to the presence of connate waters and brines hosted by the marine-to-transitional shales.

The integration of surface geological and geomorphological information with borehole point-data and geophysical (e.g., geoelectrical) images of the subsurface yields spatially consistent representations of alluvial aquifers heterogeneity at different scales, from depositional systems to basin fills. Such an approach requires a conceptual framework to match the stratigraphic units with their evidence from ground-based DC resistivity methods to effectively fill the gaps between sparse borehole data and to obtain valid representations of sedimentary heterogeneities. Such an approach is applied to characterize two sites of the Quaternary aquifers of the central Po Plain (Italy), which represent (1) the middle-upper Pleistocene braided to meandering river depositional systems sitting on Southalpine crust and (2) their down-current counterparts, where they are involved by the latest uplift and deformation due to the tectonic activity of the Apennine frontal thrusts. Electrical resistivity was considered as a proxy of the litho-textural properties of hydrofacies and their major hierarchical association at depth and was interpreted in accordance with the depth-decreasing resolution of ground-based resistivity methods. Thus, it was possible to identify the geophysical signature of hydrostratigraphic units through ''Electrostratigraphic Units'', i.e., sedimentary volumes identified by resistivity contrasts that spatially preserve the vertical polarity. Hydrostratigraphy and electrostratigraphy were then joined together through a site-specific relationship between electrical resistivity and hydraulic conductivity, which takes into account the prevailing process of current conduction, the litho-textural properties of hydrofacies and the groundwater electrical conductivity. At the scales of aquifer systems and complexes, this approach permitted to establish the conceptual framework to match hydrostratigraphy, electrostratigraphy, average hydrodynamic properties and distribution of heterogeneities.

The femoral neck fracture is actually the most important traumatic event in the elderly, because of its high rate and terrible complications. We reviwed clinical records of 314 patients treated in our institution with a bipolar implant for femoral neck fracture. At a mean follow-up of 5 years, 15 patients (4.8%) were lost to followup so data for 299 patients was studied to identity factors associated with mortality. Ten predictor variables were examined: age, sex, waiting time for surgery, pulmonary dysfunction, fracture etiology, and comorbidity with ischemic heart disease, and heart failure, hypertension, cerebrovascular disease, and chronic renal failure. Cumulative mortality rate during the first 6 months was 19% (55 of 299 patients) and in the first year it was 25% (76 of 299). At logistic regression analysis, mortality was associated with age, male gender, waiting period for surgery and presence of neoplastic disease or pathological fracture. Waiting for surgery was a significant factor for mortality at 6, 12 and 24 months: patients surgically treated in the first 24 hours had lower mortality than those who waited longer. The risk of mortality in the first 6 months doubled for an age increase of 12 years, while mortality within 2 years doubled for an age increase of 9 years. Although the motality rate after surgery for femoral neck fracture was high in the first year (25%), it dropped off in successive years to levels observed in a healthy population. Thus, we agree with the literature that femoral fracture is a risk factor for survival only in the first year after trauma, above all in the elderly.

Aims: To determine whether Ha-AP10, a member of the plant lipid transfer proteins (LTPs) family produces a direct cytotoxic effect on fungal cells mediated by membrane permeabilization. LTPs can inhibit fungal growth and are considered members of the ubiquitous class of antimicrobial peptides. However, the way they exert their effects on target cells is not yet understood. Methods and Results: Viability assays demonstrate that Ha-AP10 acts as a fungicidal compound but no harmful effect is observed on plant cells. Liposome leakage assays show that the protein induces a moderate release of fluorescent probes encapsulated in model membranes, indicating its ability to interact with phospholipids. Using a fluorescent indicator of damage at the membrane level, we demonstrate that Ha-AP10 is able to induce the permeabilization of intact fungal spores in a dose-dependent manner. Conclusion: The results presented here demonstrate the permeabilization of fungal spores caused by Ha-AP10. Significance and Impact of the Study: To our knowledge, this is the first demonstration of fungal membrane damage by an LTP, giving a clue to elucidate the basis of its antimicrobial properties.

Using an experimental approach directed to the isolation of antimicrobial proteins, we have detected the presence of a trypsin inhibitor (TI) with associated antifungal activity in sunflower seeds. Purification of the isolated protein by affinity chromatography on a trypsin-agarose matrix confirmed that a trypsin inhibitor was responsible for the inhibition of spore germination of the fungal pathogen Sclerotinia sclerotiorum. The protein is a potent antifungal compound as it can completely inhibit the germination of S. sclerotiorum ascospores at a concentration of 14 μg/ml. The putative contribution of this TI to control fungal invasion is discussed.

Fifty cases of Hodgkin's disease in intravenous drug users (IVDU) have been collected by the Italian Cooperative Group on AIDS-Related Tumors (G.I.C.A.T.). Ninety-two per cent of the patients were males; the median age was 26 years. Persistent generalized lymphadenopathy (PGL) at onset was present in 54% of patients, AIDS in 9%, ARC in 9% while 28% were simply HIV-positive. The initial median absolute number of CD4 lymphocytes was 264/mmc. Opportunistic infections were diagnosed in 20% of patients. In most patients the histological pattern was that of mixed cellularity and lymphocytic depletion (76%). In almost half the initial symptom was a persistent lymph node enlargement due to PGL. In the majority of patients (58%) only a clinical staging and bone marrow biopsy could be performed due to the presence of opportunistic infections, rapid disease progression or refusal of pathologic staging procedures. One patient presented with a Waldeyer's ring involvement, but no other unusual presentations were observed. After MOPP alternated or followed by ABVD or MOPP alone, 15/29 CR (52%) and 14/29 PR (48%) were observed. The median duration of CR was 14 months, while the median survival of CR has not been reached; the median survival of patients treated with chemotherapy with CD4 values at presentation {geq}400/mmc was significantly superior to that in those with CD4 < 400/mmc. The overall median survival was 16 months. Twenty-eight per cent of patients receiving chemotherapy + radiotherapy developed opportunistic as well as non-opportunistic infections (21%). Lethal hepatic toxicity was observed in 2 patients. In conclusion, Hodgkin's disease in IVDU was not found to be associated with unusual presentations, as previously reported for homosexuals. Complete remissions could be achieved in over 50% of patients, but in IVDU non-opportunistic infections in addition to opportunistic infections may also limit treatment administration. The presence of parenchymal functional impairment due to drug abuse, or drug abuse-related infections, such as pneumonia, endocarditis and hepatitis, should lead to the choice of antitumour agents with no or only minor potential liver, lung and cardiac toxicity.

The Ablate and Pace Trial (APT) prospectively assessed the effects of catheter ablation of the AV conduction system and permanent pacemaker implantation on health-related quality of life, survival, exercise capacity, and ventricular function in 156 patients with symptomatic atrial fibrillation.All patients referred for catheter ablation and permanent pacemaker implantation because of medically-refractory atrial fibrillation at 16 centers were screened for enrollment in a prospective registry. Baseline assessment prior to ablation included measurement of quality of life, including the Health Status Questionnaire, the Quality of Life Index and the Symptom Checklist: Frequency and Severity. Exercise capacity was assessed with metabolic treadmill exercise testing and ventricular function was quantitated with echocardiography. The quality of life instruments, exercise capacity, and echocardiography were repeated at 3 and 12 months after catheter ablation.The APT population included 90 men and 66 women (66.1 +/- 11.5 years of age) with either chronic (n = 70), recurrent (n = 31), or paroxysmal atrial fibrillation (n = 55). Structural heart disease was present in 78.2% of patients. Successful ablation of AV conduction was achieved in 155 of 156 patients (99.4%). Survival at 1 year was 85.3%, with 5 of 23 deaths being sudden cardiac deaths. Survival over the first year of follow-up was significantly lower for patients with a baseline left ventricular ejection fraction (LVEF)0.45 (0.73) than for patients with a LVEFor = 0.45 (0.88, p = 0.03). The NYHA functional class improved from 2.1 at baseline to 1.8 at 3 months and 1.9 at 12 months of followup (p = 0.0001). Significant improvement in quality of life scores were noted for all 8 subscales of the Health Status Questionnaire, for the overall rating of the Quality of Life Index, the Health and Function subscales; Arrhythmia-related symptoms were markedly reduced as measured by the Symptom Checklist: Frequency and Severity scale. The mean LVEF improved from 0.50 +/- 0.20 at baseline to 0.54 +/- 0.20 at 3 months (p = 0.03). The LVEF 12 months after ablation was 0.52 +/- 0.20, not statistically different from baseline. Individuals with reduced systolic function at baseline had the greatest improvement, from LVEF 0.31 +/- 0.20 at baseline to 0.41 +/- 0.20 at 3 months and 0.41 +/- 0.30 at 12 months (p = 0.0001). There were no significant changes in treadmill exercise duration (10.0 +/- 4.3 min at baseline and 11.6 +/- 3.6 min at 12 months) or VO2max (1467 +/- 681 ml O2 min baseline and 1629 +/- 739 ml O2 min at 12 months).Catheter ablation of the AV conduction system and permanent pacemaker implantation were associated with improved quality of life and left ventricular function in this population of highly symptomatic patients with atrial fibrillation refractory to medical therapy.

The existence of different types of imperfections in melt-grown and solution-grown KCl crystals is shown experimentally by three techniques: optical absorption in the sample after X-irradiation, depolarization thermocurrent, and thermoluminescence. The results show that the latter type of crystals contains a larger concentration of dipolar complexes and shallow traps, but a smaller concentration of vacancies and divalent impurities. Es wird das Vorhandensein verschiedener Arten von Fehlstellen in KCl-Kristallen, die entweder aus der Schmelze oder aus der Losung gezuchtet wurden, mit Hilfe der folgenden Verfahren gezeigt: optische Absorption nach Bestrahlung mit Rontgenstrahlen. Depolarisations-Thermostrome und Thermolumineszenz. Die Ergebnisse zeigen, das die letzteren Kristalltypen eine grosere Konzentration dipolarer Komplexe und flacher Storstellen jedoch eine geringere Konzentration von Leerstellen und zweiwertigen Fremdatomen besitzen.

The 180° domain‐wall motion in barium ferrite single crystals has been studied by the pulsed‐magnetic‐field technique described in a previous article.The domain‐wall velocity has been measured as a function of the internal magnetic field in a range from 50 to several hundred Oe and at temperatures of 80° and 293°K. The rate of increase of the applied magnetic field dHa/dt has no apparent effect if it does not exceed 1.6 kOe/μsec. Reasonable agreement is found between the wall velocities as measured and values foreseen on the basis of the model proposed by Hagedorn and Gyorgy to explain wall mobility in YIG.An accurate analysis of the dependence of the wall velocity on the magnetic‐field intensity suggests the existence of a threshold field Ht, which depends on the value of the damping constant α and defines the point of transition from one motion mechanism to another. We think that Ht may be identified with the critical field H0=2παMs which appears in a solution given by Walker to the domain‐wall equation...

Dynamic magnetization processes in BaO·6Fe2O3 single crystals in the easy direction are examined under rapidly varying high magnetic fields. We observe that for large values of dHa/dt (the rate of increase of the applied field) the derivative dM/dt of the sample magnetization as function of time shows a sharp structure with a maximum.A phenomenological interpretation is given, assuming that the magnetization change is only due to 180° Bloch wall displacement. In the initial stage of magnetization the number of domains remains constant and the wall velocity is directly related to the experimental results. This leads to an estimate of the viscous damping coefficient β which is found to depend very strongly on the value of the magnetic field, and is of the order of a few hundred cgs units when H reaches values comparable with the saturation magnetization Ms. Indeed in an effective field of several hundred oersteds, β appears to be several hundred times larger than it is in a field of a few oersteds. For inst...

Le soluzioni di euglobulina b, estratte da uova non fecondate di axolotl, e quelle di una frazione, che si puo estrarre con identico procedimento dal tuorlo dell'uovo di pollo, non contengono quantita apprezzabili di impurita di origine nucleare. Esse presentano aumento di viscosita ad opera di Li, diminuzione ad opera di SCN', se questi ioni vengono impiegati nelle concentrazioni attive nel produrre modificazioni dello sviluppo embrionale. Viene pertanto confermata la nostra interpretazione sul ruolo dell'euglobulina b nello sviluppo embrionale.