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Your search keyword '"Marja Veneskoski"' showing total 3 results
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3 results on '"Marja Veneskoski"'

1. Recognition of Porphyromonas gingivalis gingipain epitopes by natural IgM binding to malondialdehyde modified low-density lipoprotein.

Author

S Pauliina Turunen, Outi Kummu, Kirsi Harila, Marja Veneskoski, Rabah Soliymani, Marc Baumann, Pirkko J Pussinen, and Sohvi Hörkkö

Subjects
Medicine, Science
Abstract

OBJECTIVE:Increased risk for atherosclerosis is associated with infectious diseases including periodontitis. Natural IgM antibodies recognize pathogen-associated molecular patterns on bacteria, and oxidized lipid and protein epitopes on low-density lipoprotein (LDL) and apoptotic cells. We aimed to identify epitopes on periodontal pathogen Porphyromonas gingivalis recognized by natural IgM binding to malondialdehyde (MDA) modified LDL. METHODS AND RESULTS:Mouse monoclonal IgM (MDmAb) specific for MDA-LDL recognized epitopes on P. gingivalis on flow cytometry and chemiluminescence immunoassays. Immunization of C57BL/6 mice with P. gingivalis induced IgM, but not IgG, immune response to MDA-LDL and apoptotic cells. Immunization of LDLR(-/-) mice with P. gingivalis induced IgM, but not IgG, immune response to MDA-LDL and diminished aortic lipid deposition. On Western blot MDmAb bound to P. gingivalis fragments identified as arginine-specific gingipain (Rgp) by mass spectrometry. Recombinant domains of Rgp produced in E. coli were devoid of phosphocholine epitopes but contained epitopes recognized by MDmAb and human serum IgM. Serum IgM levels to P. gingivalis were associated with anti-MDA-LDL levels in humans. CONCLUSION:Gingipain of P. gingivalis is recognized by natural IgM and shares molecular identity with epitopes on MDA-LDL. These findings suggest a role for natural antibodies in the pathogenesis of two related inflammatory diseases, atherosclerosis and periodontitis.

Published
2012
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2. Human monoclonal Fab and human plasma antibodies to carbamyl-epitopes cross-react with malondialdehyde-adducts

Author

Piotr Prus, S. Pauliina Turunen, Jaakko Lehtimäki, Chunguang Wang, Sohvi Hörkkö, Marja Veneskoski, and Outi Kummu

Subjects
Phage display, T-Lymphocytes, Immunology, Apoptosis, Acetaldehyde, Cross Reactions, Binding, Competitive, Epitope, Epitopes, Immunoglobulin Fab Fragments, Jurkat Cells, Mice, chemistry.chemical_compound, Malondialdehyde, Animals, Humans, Immunology and Allergy, Autoantibodies, biology, Macrophages, Antibodies, Monoclonal, Original Articles, Atherosclerosis, Immunity, Humoral, Lipoproteins, LDL, Disease Models, Animal, chemistry, Biochemistry, Low-density lipoprotein, Monoclonal, biology.protein, lipids (amino acids, peptides, and proteins), Antibody, Cell Surface Display Techniques, Clone (B-cell biology), Oxidation-Reduction, Lipoprotein
Abstract

Summary Oxidized low-density lipoprotein (OxLDL) plays a crucial role in the development of atherosclerosis. Carbamylated LDL has been suggested to promote atherogenesis in patients with chronic kidney disease. Here we observed that plasma IgG and IgM antibodies to carbamylated epitopes were associated with IgG and IgM antibodies to oxidation-specific epitopes (ρ = 0·65–0·86, P

Published
2014
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3. Natural antibodies of newborns recognize oxidative stress-related malondialdehyde acetaldehyde adducts on apoptotic cells and atherosclerotic plaques

Author

Outi Kummu, Sohvi Hörkkö, Jaakko Lehtimäki, Chunguang Wang, Antti E. Nissinen, S. Pauliina Turunen, and Marja Veneskoski

Subjects
Male, Phage display, Polymers, Immunology, Apoptosis, Acetaldehyde, Protein Engineering, Epitope, Apoptotic cell clearance, Immunoglobulin Fab Fragments, chemistry.chemical_compound, Phagocytosis, Antigen, Peptide Library, Pregnancy, Malondialdehyde, otorhinolaryngologic diseases, Humans, Immunology and Allergy, Sweden, Fetus, biology, Infant, Newborn, General Medicine, Fetal Blood, Plaque, Atherosclerotic, Oxidative Stress, Immunoglobulin M, chemistry, Biochemistry, biology.protein, Female, Antibody, Clone (B-cell biology)
Abstract

Malondialdehyde acetaldehyde (MAA) adducts are generated under oxidative stress and shown to be highly immunogenic. Our aim was to investigate the recognition of MAA adducts by human natural antibodies in newborns before or at the time of full-term pregnancy. Plasma samples of pre-term (n = 11) and full-term (n = 36) newborns were enriched in specific IgM binding to MAA adducts compared with the maternal plasma IgM levels. Umbilical cord blood lymphocyte phage display library was generated to clone Fabs that specifically recognized MAA adducts without cross-reactivity to malondialdehyde. Fab clones from the antibody libraries of the pre-term and full-term newborns showed high sequence homology to the germline genes encoding the variable regions of antibodies, confirming that these Fabs represented the natural antibody repertoire of human fetuses. The MAA-specific umbilical cord blood Fabs bound to apoptotic human endothelial cells and the binding was efficiently competed with MAA adducts. The MAA-specific Fabs also recognized epitopes on advanced atherosclerotic lesions, and the uptake of infrared (IR)-labeled MAA-low-density lipoprotein by mouse J774A.1 macrophages was significantly reduced in the presence of these Fabs. In conclusion, MAA adducts were identified as one of the major antigenic targets for human natural antibodies already before the time of birth. MAA-specific natural antibodies are suggested to regulate apoptotic cell clearance starting from fetal development and to participate in the immunomodulation of atherosclerosis development during adulthood.

Published
2013
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