1. Direct Detection of Low-Molecular-Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry
- Author
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Angéline Van der Heyden, Anthony Vignon, Jérôme Dejeu, Mehdi Djeghdir, Eric Defrancq, Liliane Coche-Guérente, Nicolas Spinelli, Maxime Michelas, Arthur Flaget, Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Ingéniérie et Intéractions BioMoléculaires (DCM - I2BM), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Analyte ,Bio-Layer Interferometry ,Aptamer ,Bioengineering ,Biosensing Techniques ,02 engineering and technology ,Ligands ,01 natural sciences ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,aptasensor ,Instrumentation ,Fluid Flow and Transfer Processes ,Low Molecular Weight ,Chemistry ,label free detection ,Process Chemistry and Technology ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,Ligand (biochemistry) ,0104 chemical sciences ,[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry ,Kinetics ,small analyte ,Interferometry ,optical technique ,Biophysics ,L-Tyrosinamide ,0210 nano-technology - Abstract
International audience; The direct Bio-Layer Interferometry (BLI) meas-urementof low molecular weight analytes (less than 200 Da) still represents a challenge particularly whenlow receptordensities are used. Bio-Layer Interferometry (BLI) is a powerful optical tech-nique for label-free, real-time characterization and quantification of biomolecular interactions at interfaces. We demonstrate herein that the quantification of biomolecular recognition is possible by BLI using either 2D-like or3D platforms for an aptamerligandimmobilization. The influence of the aptamer density on the inter-action was evaluated and compared for the two sensors architec-tures. Despite the low molecular weight (LMW)of the analyte, BLI monitoring led to signals that are exploitablefor affinity and kinetic studies even atlow aptamer density. We demonstrate that the immobilization format as well as the aptamer density hasa crucial influence on the determination of the recognition parame-ters.
- Published
- 2020
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