1. Study of modified Sephadex-bound insulin in animal experiments
- Author
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Mazaev Av, Vladimir P. Torchilin, B. S. Lebedev, V.N. Smirnov, Il'ina Ev, and Chazov Ei
- Subjects
Reaction conditions ,Chromatography ,Chemistry ,Insulin ,medicine.medical_treatment ,Biological activity ,Applied Microbiology and Biotechnology ,Biochemistry ,Sephadex ,Homogeneous ,Covalent bond ,medicine ,Bound insulin ,Biotechnology - Abstract
Preparations of insulin covalently coupled to Sephadex were obtained using modified aldehyde-containing Sephadex carriers. In all cases carriers used had the same structure but differed in molecular weight. Depending on the reaction conditions insulin might or might not be split from the carrier. In animal experiments it was shown that in the homogeneous state all preparations retain high biological activity independent of molecular weight of the carriers, which varied from 10,000 to 150,000. The binding of insulin with polymeric carriers prolonged the time of its action from 5-6 to 14-16 h.
- Published
- 1977
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