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Progressive developments in industrial and agricultural activities are causing a critical stress on groundwater quality in developing countries. The objective of this paper is to assess and evaluate the contamination level of groundwater caused by leachate in 11 villages of the Gautam Budh Nagar district in Uttar Pradesh, India. We systematically sampled 22 groundwater samples and 13 leachate samples to ascertain the source of pollution on groundwater quality. The standard analytical methods given by the American Public Health Association (APHA) (Standard methods for examination of water and wastewater, 23rd edn. APHA, AWWA, WPCF, Washington, 2017) were used for quantitative estimation of hydrochemical parameters of collected samples. The results of the analysis of groundwater samples indicate that pH values range from 7.31 to 8.97. The mean magnesium concentration in groundwater samples is 58.93 ± 21.44 mg/L. Out of the groundwater samples taken, approximately 41% and 73% of samples analysis results have been found beyond the acceptable limit with respect to the parameters of turbidity and total dissolved solids, respectively, according to the Bureau of Indian Standards (Indian standard specification for drinking water (IS:10500). BIS, Manak Bhawan, New Delhi, 2012) for drinking water. Around 95.4% of groundwater samples and 92.3% of leachate samples have high nitrate concentrations above the standard limit of BIS (45 mg/L), respectively. The Piper plot shows that 50% of the samples belong to the Ca2+–Mg2+–HCO3− type. Ternary and Durov’s diagrams indicate that the mean concentrations of ions are in the order of Na+ > Mg2+ > Ca2+ > K+ (for cations) and HCO3−> NO3− > Cl− > SO42− > CO32− > F− (for anions) in groundwater of the study area. The spatial variation of the hydrochemical parameters shows that groundwater is heavily contaminated with respect to nitrate. Analytical results indicate that the groundwater of villages Achheja, Bisrakh road, Dujana, Badalpur and Sadopur is not suitable for drinking.

Background: Buffalo is the major source of animal protein in south-east Asia, including India; therefore, the presence of multiple drug resistance in Salmonella strains of buffalo meat and milk products is of immense public health concern. Methodology: Forty-six strains of Salmonella enterica subspecies enterica belonging to eight serovars ( S. Anatum, 13; S. Weltevreden, 13; S. Rostock, 6; S. Typhimurium, 5; S. Gallinarum, 5; S. Stockholm, 1; S. Dublin, 1; and S. Orion, 2), isolated from buffalo meat and diseased buffaloes were studied for their antibiotic sensitivity and plasmid profile. Results: All except six strains of Salmonella had one or more plasmids. Virulence plasmid of ~35MdA was present in 39 isolates while 19 strains had one to six additional plasmids with molecular weight ranging from 1 Mda > 35 Mda. A plasmid-free S. Anatum strain was resistant to seven drugs including fluoroquinolones, while strains having six to seven plasmids were resistant to fewer antimicrobial drugs. One S . Anatum isolate, resistant to 11 antibiotics, had only one plasmid. Eight serovars of Salmonella could be divided into 28 resistotypes on the basis of antimicrobial sensitivity assay. Most strains were resistant to streptomycin (84.8%) followed by kanamycin (58.7%), gentamicin (52.2%), ampicillin (50%) and oxytetracycline (50%). Few strains were resistant to cefotaxime (2.2%), amoxycillin (2.2%) and newer fluoroquinolones (6.5%). Conclusion: Multiple drug resistance was common among Salmonella isolates of buffalo origin, particularly against aminoglycosides, oxytetracycin, ampicillin and cephalexin. Presence of plasmids is not mandatory for occurrence of multiple drug resistance in S. enterica strains.

We screened serum samples of 1024 goats slaughtered for chevon in Bareilly in Northern India for Salmonella antibodies with indirect ELISA, MAT-H (microagglutination test using flagellar antigens e, n, x and 1, 5) and MAT-O (microagglutination test using somatic antigens 4, 12 and 3, 10, 15). Salmonella antibodies were detected in 48, 8 and 40%, goats using Salmonella-cytotoxi-I ELISA, MAT 'H' and MAT 'O', respectively. After adjusting for test accuracy, the seroprevalence were highest for Salmonella-cytotoxi-I ELISA (46%) followed by agglutinins against 'O' 3, 10, 15 (15%) and negligible for other agglutinins. With all 5 tests, prevalence of Salmonella antibodies was significantly higher in females than in males. No significant difference was evident in prevalence of Salmonella antibodies to different antigens in different age groups of male goats except for e, n, x agglutinins that were significantly more prevalent in young adult (6-18 months) males than in adult (18 months of age) or young (or =6 months of age) goats. On the other hand, in females, prevalence of Salmonella-cytotoxin-I antibodies and e, n, x agglutinins differed significantly among three age groups, being the most prevalent in adult goats. As expected, the results of different tests had little or no correlation because the different tests targeted antibodies to different antigens.

Out of 204 slaughtered goats examined, 35(17.6%) yielded Salmonella. Salmonella could be isolated either from mesenteric lymph nodes (11) or from gall bladder (15) or from both of the organ (9) of the goats examined. A total of 60 Salmonella isolates included 34 from gall bladder (of 24 goats) and 26 from mesenteric lymph node (of 20 goats) samples. On serotyping, 29 isolates were found to be rough strains while 31 could be serotyped in to 17 serovars, viz. one each to S. Paratyphi B var Java, S. Altona, S. Weltevreden, S. Rovaniemi, S. Sarajane, S. Louga, S. I. 52: r: 1, 5; S. I. 1, 3, 19: r: z6; S. I. 3, 19: r: 1, 5; S. I. 28: b: e, n, x and S. I. 6, 7: r: Z6, two each to S. Chicago, S. Czernyring, S. Kirkee, and S. I. 3, 10: z6: -; five to S. Paratyphi B and seven to S. I. 28: b: 1, 2. Of these serovars, S. Czernyring, S. Louga, S. Rovaniemi, S. Kirkee, S. Sarajane, S. Altona have not been reported earlier from India. On the basis of drug resistance, the isolates could be classified into 40 resistotypes. All the isolates were sensitive to imipenem and chloramphenicol, while 70% were resistant to nitrofurantoin. Multiple drug resistance (resistant to >3 drugs) was evident in 51.67% isolates (31) while 13.3% (8) isolates were sensitive to all the drugs evaluated under the study.