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Simulated experiment was carried out to investigate the alexipharmic effect and mechanism of 1, 8 - naphthalic anhydride (NA) on the imazethapyr residues in soil using potted rape as bioassay material. The rape seedling height, fresh weight, activity of glutathione - S - transferase (GST), amount of amino acids, and content of glutathione (GSH) were reduced with 25g ai/hm² imazethapyr. The damage was alleviated under seed dressing with 0.5% (seed weight) NA. Compared tothe treatment of imazethapyr, rape seedling height and fresh weight were increased under NA + imazethapyr. The amount of amino acids, content of GSH, and activity of GSTs were induced by both NA and imazethapyr. Under NA + imazethapyr, 16 amino acids (except proline) were increased, GSH was slightly accumulated, while GST activity was significantly stimulated. Results indicated that imazethapyr detoxification was resulted from high amount of amino acid and more active GSTs. [ABSTRACT FROM AUTHOR]