Objective To observe the changes of Han male pubic symphysis surface morphology with different age in Yunnan province, analyze the correlation between pubic symphysis surface morphology and age, and establish an equation for inferring age. Methods We collected 85 Yunnan Han male pubic specimens in daily work, their ages between 17-50 years, and had exact records about ages (specimens of all lesions and removed without damage). We selected six morphological changes in the project to observe and develop appropriate assessment criteria, based on the standard morphological changes on the surface of the pubic symphysis to score, and then usd multiple linear regression method for processing the data obtained, finally, established an equation for inferring age according to Yunnan male pubic symphysis surface morphology. Results With the above method, we established the equation for inferring age according to Yunnan male pubic symphysis surface morphology: = 15.884 + 1.466 X1 + 1.777 X2 + 2.040 X4 + 1.504 X5 + 3.006 X6. Conclusion The equation we established is more better than before in practical applications. [ABSTRACT FROM AUTHOR]