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Large studies in von Willebrand disease patients, including Spanish and Portuguese registries, led to the identification of >250 different mutations. It is a challenge to determine the pathogenic effect of potential splice site mutations on VWF mRNA. This study aimed to elucidate the true effects of 18 mutations on VWF mRNA processing, investigate the contribution of next-generation sequencing to in vivo mRNA study in von Willebrand disease, and compare the findings with in silico prediction. RNA extracted from patient platelets and leukocytes was amplified by RT-PCR and sequenced using Sanger and next generation sequencing techniques. Eight mutations affected VWF splicing: c.1533+1G>A, c.5664+2T>C and c.546G>A (p.=) prompted exon skipping; c.3223-7_3236dup and c.7082-2A>G resulted in activation of cryptic sites; c.3379+1G>A and c.7437G>A) demonstrated both molecular pathogenic mechanisms simultaneously; and the p.Cys370Tyr missense mutation generated two aberrant transcripts. Of note, the complete effect of three mutations was provided by next generation sequencing alone because of low expression of the aberrant transcripts. In the remaining 10 mutations, no effect was elucidated in the experiments. However, the differential findings obtained in platelets and leukocytes provided substantial evidence that four of these would have an effect on VWF levels. In this first report using next generation sequencing technology to unravel the effects of VWF mutations on splicing, the technique yielded valuable information. Our data bring to light the importance of studying the effect of synonymous and missense mutations on VWF splicing to improve the current knowledge of the molecular mechanisms behind von Willebrand disease. clinicaltrials.gov identifier:02869074.

The multimeric analysis (MA) of plasma von Willebrand factor (VWF) evaluates structural integrity and helps in the diagnosis of von Willebrand disease (VWD). This assay is a matter of controversy, being considered by some investigators cumbersome and only slightly informative. The centralised study 'Molecular and Clinical Profile of von Willebrand Disease in Spain (PCM-EVW-ES)' has been carried out by including the phenotypic assessment and the genetic analysis by next generation sequencing (NGS) of the VWF gene (VWF). The aim of the present study was to evaluate the role of MA to the diagnosis of these patients and their potential discrepancies. Two hundred and seventy out of 480 patients centrally diagnosed with VWD had normal multimers, 168 had abnormal multimers and 42 a total absence of multimers. VWF MA was of great significance in the diagnosis of 83 patients (17.3%), it was also of help in the diagnosis achieved in 365 additional patients (76%) and was not informative in 32 cases (6.7%). With regard to discrepancies, 110 out of 480 (23%) patients centrally diagnosed with VWD presented some kind of discordance between VWF:RCo/VWF:Ag and/or VWF:CB/VWF:Ag ratios, multimeric study and/or genetic results. The VWF MA was key in the presence of novel mutations as well as in cases with phenotypic discrepancies. A comparison between the contribution of MA and VWF:CB showed a clearly higher contribution of the former in the diagnostic process. These data seem to reinforce the relevance of the VWF MA in VWD diagnosis, despite all its limitations.

Molecular diagnosis of patients with von Willebrand disease is pending in most populations due to the complexity and high cost of conventional molecular analyses. The need for molecular and clinical characterization of von Willebrand disease in Spain prompted the creation of a multicenter project (PCM-EVW-ES) that resulted in the largest prospective cohort study of patients with all types of von Willebrand disease. Molecular analysis of relevant regions of the VWF, including intronic and promoter regions, was achieved in the 556 individuals recruited via the development of a simple, innovative, relatively low-cost protocol based on microfluidic technology and next-generation sequencing. A total of 704 variants (237 different) were identified along VWF, 155 of which had not been previously recorded in the international mutation database. The potential pathogenic effect of these variants was assessed by in silico analysis. Furthermore, four short tandem repeats were analyzed in order to evaluate the ancestral origin of recurrent mutations. The outcome of genetic analysis allowed for the reclassification of 110 patients, identification of 37 asymptomatic carriers (important for genetic counseling) and re-inclusion of 43 patients previously excluded by phenotyping results. In total, 480 patients were definitively diagnosed. Candidate mutations were identified in all patients except 13 type 1 von Willebrand disease, yielding a high genotype-phenotype correlation. Our data reinforce the capital importance and usefulness of genetics in von Willebrand disease diagnostics. The progressive implementation of molecular study as the first-line test for routine diagnosis of this condition will lead to increasingly more personalized and effective care for this patient population.

Introducción: El paro cardiorrespiratorio es la documentación de la pérdida del pulso y respiración. La resucitación cardiopulmonar (RCP) está compuesta por compresiones torácicas interpuestas por ventilaciones, las cuales pueden producir el retorno de circulación espontánea, un ritmo y pulso viable. Los parámetros de cómo realizar RCP están definidas por guías consensuadas, pero estos no son rutinariamente medidos, por lo que la calidad es desconocida. Objetivos: Determinar el grado de conocimiento teórico y práctico del manejo del paro cardiorrespiratorio en estudiantes de 6to. año de la Facultad de Medicina de la Universidad Francisco Marroquín utilizando simuladores clínicos. Metodología: Estudio descriptivo y abierto, donde se incluyeron 26 estudiantes usando simuladores de alta fidelidad de RCP, las cuales proveen respuestas fisiológicas realísticas, por medio de algoritmos matemáticos generados por programas en computadoras. Resultados: De los 26 estudiantes, 0% aprobó el examen teórico con un promedio de 77.4 puntos; en la evaluación práctica ninguna pareja logró una efectividad mayor al 80%. El promedio de la profundidad de las compresiones fue de 3.38 cm, el promedio de liberación post-compresión fue de 51% y el promedio de tiempo de interrupción total fue 79 segundos. El análisis estadístico demostró que no hay relación entre los resultados del test teórico y la efectividad en las compresiones y ventilaciones en el manejo del paro cardiorrespiratorio. Conclusiones: Se pudo evidenciar que los estudiantes no tienen la competencia apropiada en Soporte Vital Básico y Avanzado, por lo que se recomienda enfocar los cursos de Advance Cardiac Life Support (ACLS) a un mayor abordaje práctico con simuladores fisiológicos y que los estudiantes realicen el curso en periodos de tiempo más seguidos.

El sobrepeso y la obesidad afectan a todos los países alrededor del mundo, es común observar su inicio durante la niñez. Es secundario tanto a factores ambientales como genéticos. (1, 2) Se ha asociado a un aumento en la morbilidad y mortalidad de enfermedades, como diabetes mellitus y enfermedades cardiovasculares. (3) Podemos evaluarlo de forma sistemática midiendo el índice de masa corporal y a partir de ello clasificarlo y así calcular los riesgos para el paciente. Objetivo: Determinar la prevalencia de sobrepeso y obesidad en estudiantes de la Facultad de Medicina de la Universidad Francisco Marroquín. Detallar el peso y talla de los estudiantes. Establecer una base de datos con las medidas antropométricas de los estudiantes. Evaluar los factores de riesgo para enfermedad cardiovascular asociados a sobrepeso y obesidad. Metodología: Transversal observacional. Se pesó y midió a 134 estudiantes de primer a tercer año de la Facultad de Medicina, luego se calculó el índice de masa corporal y se clasificó a los estudiantes en bajo peso, normal, sobrepeso y obesidad. Resultados: De los 134 estudiantes se encontró que 11% de estudiantes con bajo peso, 55% en peso normal, 28% en sobrepeso y 6% de estudiantes en obesidad. Conclusión: Se encontró que la prevalencia de sobrepeso y obesidad es parecida a la de estudios realizados en estudiantes de otros países, tanto en hombres como en mujeres. Se pudo observar que la relación de uso de cigarrillo, alcohol y aumento de presión arterial, con sobrepeso y obesidad no es significativa y se concluyó que pudo ser debido al tamaño de la muestra y que más adelante, con el crecimiento de esta, la relación puede cambiar.

La presente investigación partió de la premisa de que, si bien es cierto que la pobreza es tan antigua como el género humano, es el polo opuesto de la riqueza. La pobreza depende del desarrollo relativo alcanzado por determinada región o país; razón por la cual existen tantas definiciones de pobreza y maneras de medirla como aspectos o criterios existan, que han dado origen a planes, programas y lineamientos de Lucha Contra la pobreza que poco o nada han permitido la reducción significativa de la pobreza, especialmente en las zonas rurales, donde la población vive en medio de grandes potencialidades expresadas en capitales: humano, natural, físico y social, que no les ofrecen ningún tipo de oportunidades para iniciar un proceso de desarrollo humano sostenible. La pregunta es ¿por qué la población rural continúa viviendo en medio de una pobreza generalizada, persistente y intergeneracional, la misma que se constituye en barrera para el proceso de desarrollo humano sostenible que le posibilite llevar una vida digna?

El polvo desecado del cartílago de tiburón constituye un producto natural, al que se le atribuyen diferentes acciones biológicas y ha sido muy utilizado. En este trabajo se realizó una revisión bibliográfica. El cartílago de tiburón evidentemente posee particulares características estructurales que le diferencian del cartílago de los vertebrados superiores y el hombre. Han sido sometidas a estudio y comprobadas las acciones analgésica e inhibidora de la angiogénesis. En los últimos 3 años ha sido demostrado que los glucosaminoglucanos del cartílago de tiburón muestran una actividad moduladora del crecimiento y desarrollo de neuronas en cultivo de tejidos. Otras acciones biológicas investigadas fueron: acción antiinflamatoria, antimutagénica, activadora de la respuesta inmunológica y antilipemiante. Se reconoce a Cuba entre los primeros en su uso en la terapia complementaria del cáncer avanzado refractario. No obstante, resulta necesario que su empleo con finalidades terapéuticas se fundamente con ensayos clínicos, que no han resultado concluyentes hasta el momentoThe shark cartilage powder is a widely used natural product to which a number of biological actions have been attributed. This paper set forth a literature review. Obviously, the shark cartilage has particular structural features that make it different from other Vertebrata´s cartilages and the man´s cartilage. Analgesic and inhibitory actions of angiogenesis have been studied and tested. Over the last 3 years, it has been demonstrated that shark cartilage glycoaminoglucans show modulating action on the growth and development of neurons in tissue culture. Other researched biological actions are: anti-inflammatory, anti-mutagenic, immune response-activating and anti-lipemia actions. It is acknowledged that Cuba was one of the first countries to use shark cartilage in the supplementary therapy for advanced refractory cancer. However, it is required that its use for therapeutic purposes be based on clinical assays because such assays have not been conclusive so far

Se estudió el impacto del tabaquismo como factor de riesgo aterogénico en pacientes fallecidos y que se les había realizado autopsia, de edades comprendidas entre 5 y 34 años. Esta investigación multinacional de la Organización Mundial de la Salud (OMS) y la Federación Internacional de Sociedades de Cardiología se desarrolló durante 10 años, desde 1986 hasta 1996. Del Centro de Coordinación General de esta investigación en Malmö, se recibieron en el Centro de Investigaciones y Referencias de Aterosclerosis de La Habana (CIRAH) un total de 966 mitades izquierdas de la aorta torácica, 947 mitades izquierdas de la aorta abdominal y 959 arterias coronarias derechas. Las arterias procedieron de 11 países localizados en 5 regiones de la OMS, América, África, Europa, Sudeste Asiático y Sudoeste Asiático. Las arterias se procesaron con la utilización de la metodología propuesta por la OMS desde 1957 para la aplicación del sistema arterométrico (SA), conjunto de métodos y procedimientos considerados idóneos para la caracterización patomorfológica y morfométrica de la lesión aterosclerótica. La población de autopsias se dividió en 2 grupos fumadores y no fumadores. Los datos se procesaron mediante análisis estadísticos descriptivos, comparativos y multivariados. Entre las conclusiones más importantes se mencionan las siguientes: la distribución de estrías adiposas y placas fibrosas en las 3 arterias estudiadas, aorta torácica, aorta abdominal y coronaria derecha fue mayor en los fumadores que en los no fumadores. La transformación de estrías adiposas en placas fibrosas comenzó más temprano y con mucha mayor intensidad en los fumadores. El estadístico MANOVA entre fumadores y no fumadores mostró gran significación en las 3 arterias. El resultado del estadístico ANOVA mostró significación de las estrías adiposas en la aorta torácica, y de estas y las placas fibrosas en la aorta abdominal, y de las placas fibrosas en la coronaria derecha. En esta investigación quedó claramente demostrado que el tabaquismo es un fuerte factor de riesgo de aterosclerosis y por lo tanto, de sus consecuencias orgánicas, las enfermedades cardiovasculares en edades tempranas.The impact of smoking as an atherogenic risk factor in dead patients aged 5-34 that underwent necropsy was studied. This multinational investigation was carried out by the World Health Organization and the International Federation of Cardiology Societies from 1986 to 1996. The Center for General Coordination of this research in Malmö, sent 966 left halves of thoracica-orta, 947 left-halves of the abdominal aorta and 959 right coronary arteries.The arteries came from ll countries located in 5 regions of the World Health Organization: America, Africa, Europe, Southeastern and Southwestern Asia. These arteries were processed by using the methodology proposed by the World Health Organization since 1957 for the application of the atherometric system (AS), a set of methods and procedures considered as ideal for the pathomorphologic and morphometric characterization of the atherosclerotic lesion. The population of autopsies was divided into 2 groups: smokers and non smokers. Data were processed by statistical descriptive, comparative and multivariate analyses. Among the most important conclusions are the following: the distribution of adipose strias and fibrous plaques in the 3 studied arteries, thoracic aorta, abdominal aorta and right coronary artery was greater in smokers than in non smokers. The transformation of adipose strias into fibrous plaques began earlier and with much more intensity among smokers. The ANOVA statistical analysis between smokers and non smokers had a great signification in the 3 arteries. The result of the ANOVA statistical analysis showed the importance of the adipose strias in the thoracic aorta and of these strias and the fibrous plaques in the right coronary artery. It was clearly proved in this research that smoking is a strong risk factor for atherosclerosis and, therefore, for its organic consequences, the cardiovascular diseases at early ages.

Bcl-2-associated athanogene 3 (BAG3) plays an important function in cellular protein quality control (PQC) maintaining proteome stability. Mutations in the BAG3 gene result in cardiomyopathies. Due to its roles in cardiomyopathies and the complexity of BAG3–protein interactions, it is important to understand these protein interactions given the importance of the multifunctional cochaperone BAG3 in cardiomyocytes, using an in vitro cardiomyocyte model. The experimental assay was conducted using high pressure liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in the human AC16 cardiomyocyte cell line with BioID technology. Proteins with BAG3-interaction were identified in all the 28 hallmark gene sets enriched in idiopathic cardiomyopathies and/or ischemic disease. Among the 24 hallmark gene sets enriched in both idiopathic cardiomyopathies and ischemic disease, 15 gene sets had at least 3 proteins with BAG3-interaction. This study highlights BAG3 protein interactions, unveiling the key gene sets affected in cardiomyopathies, which help to explain the molecular mechanisms of the cardioprotective effects of BAG3. In addition, this study also highlighted the complexity of proteins with BAG3 interactions, implying unwanted effects of BAG3. [ABSTRACT FROM AUTHOR]

Copyright of Avaliação Psicológica is the property of Instituto Brasileiro de Avaliacao Psicologica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Avances en PsicologÍa Latinoamericana is the property of Colegio Mayor de Nuestra Senora del Rosario and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Intestinal invagination occurs when an intestinal segment is introduced into another segment. The appendicular presentation is less common and affects predominantly infants. The diagnosis is corroborated during the surgical intervention which consists of de-invagination followed by surgical removal of the appendix, if the latter is not possible then a wide resection or right hemicolectomy is indicated. We present the case of a 4-year-old girl who attended with a history of abdominal pain, anorexia, vomiting and fever; an abdominal ultrasound showed intestinal agglomeration and a rounded mass. The surgical findings included appendicular invagination that affected the cecum, the lesion was resected until the ascending colon. An ileostomy was performed, the anatomopathological findings indicated necrosis of the appendix. Three months later the normal intestinal transit was restored. Appendicular intussusception has non-specific symptoms and could be mortal in Peru. A detailed clinical history may help in diagnosing and offering proper treatment. [ABSTRACT FROM AUTHOR]

Copyright of Avaliação Psicológica is the property of Instituto Brasileiro de Avaliacao Psicologica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Gerais: Revista Interinstitucional de Psicologia is the property of Gerais: Revista Interinstitucional de Psicologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Although there are few Brazilian studies on this subject, envy is a universal phenomenon present in the cultural and social spheres. This research aimed to conduct a cross-cultural adaptation to Brazilian Portuguese and to evaluate validity evidence for the Benign and Malicious Envy Scale (BeMaS), a measure of benign and malicious envy. A total of 248 adolescents aged between 12 and 17 (M=13.93 ±1.39, 47.9% boys) participated in this research. Evidence of validity based on content, internal structure, relationship to other variables (stress, anxiety, depression, and life satisfaction), and reliability indicators were estimated. The exploratory factor analysis suggested the adequacy of the internal structure composed of two factors, demonstrating the equivalence of the internal structure with the original version and good indicators of reliability (ω= 0.760 e 0.823) and relationships with external variables consistent with the theoretical hypotheses. The Results suggest the adequacy of the instrument. [ABSTRACT FROM AUTHOR]

Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly, and posttranslational modifications (PTMs). Here we aimed to identify the machinery supporting recombinant protein secretion by measuring the protein–protein interaction (PPI) networks of four different recombinant proteins (SERPINA1, SERPINC1, SERPING1, and SeAP) with various PTMs and structural motifs using the proximity‐dependent biotin identification (BioID) method. We identified PPIs associated with specific features of the secreted proteins using a Bayesian statistical model and found proteins involved in protein folding, disulfide bond formation, and N‐glycosylation were positively correlated with the corresponding features of the four model proteins. Among others, oxidative folding enzymes showed the strongest association with disulfide bond formation, supporting their critical roles in proper folding and maintaining the ER stability. Knockdown of disulfide‐isomerase PDIA4, a measured interactor with significance for SERPINC1 but not SERPINA1, led to the decreased secretion of SERPINC1, which relies on its extensive disulfide bonds, compared to SERPINA1, which has no disulfide bonds. Proximity‐dependent labeling successfully identified the transient interactions supporting synthesis of secreted recombinant proteins and refined our understanding of key molecular mechanisms of the secretory pathway during recombinant protein production. [ABSTRACT FROM AUTHOR]

Copyright of Building the way - Revista do Curso de Letras da UEG/Itapuranga is the property of Universidade Estadual de Goias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to investigate the evidence of validity and reliability of the Inventory of Coping for Athletes in a Competition Situation (ICASC-40). This instrument evaluates the coping strategies employed by athletes experiencing stressful situations inherent to sport environments through two broad dimensions: approximation and avoidance. The sample was composed of 243 athletes of different types of sports, aged between 14 and 47 years. (M=21.66±6.65, 55.6% men), enrolled in federative sport institutions. Exploratory Factor Analysis revealed a two-dimensional structure for the ICASC-40, confirming the theoretical hypothesis of the strategies of avoidance and approximation. Confirmatory Factor Analysis showed the adequacy of the ICASC-40 for the evaluation of specific coping strategies, with satisfactory indexes of internal consistency. Furthermore, weak and moderate magnitude correlations were found with adaptive efficacy, indicating adequacy of the instrument to measure the coping construct in athletes. [ABSTRACT FROM AUTHOR]

The inseparability between health and the environment is essential for the better performance of nurses, still seen in training as a peripheral theme. The way the student perceives the environment indicates how it will act in relation to care for the environment and its impacts on the health of the population. The way the professional takes care of this environment depends on the environmental perception that it has of it. This is a descriptive case study with a qualitative approach. Overall objective: Identify the way nursing students perceive the environment. The semi-structured interview for data collection was used. The results indicated that the predominant environmental perception is reductionist, which may impact on its actions and responsibilities on this medium. It is necessary to overcome the reductionist perception, from the nursing graduation, sensitizing students to act responsibly with the environment in which they live and their actions to promote human health. [ABSTRACT FROM AUTHOR]

RESUMO O estudo teve como objetivo adaptar a Optimal Functioning in Society Scale (OFISS) à língua portuguesa brasileira e estimar as primeiras evidências de validade, baseadas na estrutura interna e relação com outras variáveis e precisão. A amostra foi composta por 228 participantes, com idades entre 18 e 72 anos (M=32,3, DP=12,4), sendo 71,5% do sexo feminino. A análise dos juízes, por meio do coeficiente de validade de conteúdo, indicou a adequação dos itens em relação a clareza de linguagem, pertinência prática e relevância teórica. A análise fatorial exploratória demonstrou a adequação da estrutura oblíqua de seis fatores, todos com bons indicadores de precisão, semelhante a proposta original. Por fim, os fatores da OFISS relacionaram-se positivamente com satisfação com a vida e afetos positivos, e negativamente com os afetos negativos. Os resultados sugerem que a OFISS é uma medida adequada do funcionamento ótimo na população brasileira.

Objective: To identify the rate of fetal mortality by Congenital Syphilis in pregnant patients of Obstetrics and Gynecology service of the Hospital Nacional Hipolito Unanue (HNHU) during the months of January-August 2013. Material and Methods: It is an observational, descriptive, longitudinal, retrospective study. It was executed in HNHU of III - 1 category of the Ministry of Health, during June-September 2013. The studied population was the pregnant patients with fetal death due to Congenital Syphilis of the Obstetrics and Gynecology service of of HNHU during the months of January to August 2013. The considered sample size was the whole universe. The data analysis was quantitative with descriptive level. The used statistical packages were Microsoft Excel 2010 and SPSS 19. Results: From 33 congenital syphilis cases, 12 ended in fetal death and 21 in live births. The incidence of syphilis in pregnant women in the study was 2.9 %. Conclusions: The rate of fetal mortality by congenital syphilis in pregnant patients of Obstetrics and Gynecology service of the Hospital Nacional Hipolito Unanue (HNHU) during the months of January to August 2013 was 2.4 %. [ABSTRACT FROM AUTHOR]

ABSTRACT Objective To estimate hepatitis A vaccination coverage in 24-month-old children and identify factors associated with non-vaccination. Methods This was a survey involving a sample stratified by socioeconomic strata in capital cities (2020-2022), with coverage estimates and 95% confidence intervals (95%CI), the factor analysis was performed using the prevalence ratio (PR) by means of Poisson regression. Results Among 31,001 children, hepatitis A coverage was 88.1% (95%CI 86.8;89.2). Regarding socioeconomic strata (A/B), the variable immigrant parents/guardians was associated with non-vaccination (PR = 1.91; 95%CI 1.09;3.37); in strata C/D, children of Asian race/skin color (PR = 4.69; 95%CI 2.30;9.57), fourth-born child or later (PR = 1.68; 95%CI 1.06;2 .66), not attending daycare/nursery (PR = 1.67; 95%CI 1.24;2.24) and mother with paid work (PR = 1.42; 95%CI 1.16;1.74) were associated with non-vaccination. Conclusion Hepatitis A coverage was below the target (95%), suggesting that specificities of social strata should be taken into consideration.

Durante o período de distanciamento físico na pandemia da covid-19, a prática de exercício físico apresentou funções essenciais na manutenção da saúde. Esta pesquisa teve como objetivo avaliar o poder preditivo das dimensões da paixão (obsessiva [PO] e harmoniosa [PH]) sobre o tempo de prática durante a semana, o estado de mindfulness na realização do exercício e suas consequências sobre a satisfação com a prática. Participaram do estudo 359 praticantes regulares de exercício de ambos os sexos (67.4 % mulheres), com idades entre 18 e 70 anos (M = 36.6 ± 11.9). Foram empregadas análises de correlação de Pearson e modelagem de equações estruturais. Os resultados evidenciaram que ambas as dimensões da paixão prediziam positivamente o tempo de prática, contudo apenas a PH predizia o estado de mindfulness, o qual predizia positivamente a satisfação com a prática. Os resultados corroboram a literatura sobre o modelo dualístico da paixão ao indicar a PH como uma variável promotora do funcionamento psicológico positivo.

Abstract Objective of this study was to, based on a theoretical model, verify the associations between the variables passion, affect, satisfaction with life, and distress, the predictive power of the different types of passion on the perception of positive and negative affect, and the effect of this relation against the evaluation of psychological well-being and distress. Method Three hundred and fifty-nine participants aged between 18 and 70 years (M = 36.60; SD = 11.90) answered a sociodemographic questionnaire, and the Passion, Positive and Negative Affect Schedule, Satisfaction with Life, and the Kessler Psychological Distress Scales. Data were analyzed using correlation coefficients and structural equation modeling. Results The adequacy of the proposed model was estimated with satisfactory fit indices and positive relations between Harmonious Passion, Positive Affect, and Satisfaction with Life. Conclusion The results suggest that Harmonious Passion contributes to coping with suffering and negative experiences, such as those experienced in the pandemic moment.