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Background: During the surge of COVID-19 pandemic, the mechanism of reporting and documentation of deaths was inadequate. It is crucial to establish the mechanism for measuring the actual events of COVID-19 and associated mortalities as well during this pandemic situation. This is the first instance of application of different rapid methods for death estimation during the pandemic situation. If found to be valid, these methods may be useful for other countries with limited resources and less developed death registration system. Objective: To find out the feasibility, acceptability of different rapid method for estimating the cause of deaths among the general population & healthcare workers and also to estimate the number of deaths among allopathic doctors. Methodology: The study was conducted from September 2022 to October 2023. Target population being adult deceased person between 1st April 2020 to 30th November 2022 among general population, health care worker and allopathic doctor. For the general population, the study was conducted in one urban municipal area and two sub-centres of Habra Block I under North 24 Parganas district with WHO Verbal Autopsy (VA) tool for adult. Complete enumeration with snowballing, compartmental and capture-recapture method was adopted for general population, health care worker and allopathic doctor respectively. Results: Overall, 300 VA could be performed (121 urban & 179 rural) among general population. There were challenges related to both feasibility & acceptability in terms of data access, permission and cooperation. Research team employed a combination of persistence, local collaboration, and strategic decision-making to successfully collect data. Acceptability was more in rural area. Total number of deceased doctors was estimated to be 246 from two different lists. Conclusion: Rapid methods may be employed as a part of response systems during pandemic or any future disasters if adequate governmental support and assistance is obtained along with aid from the local people.

Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis. Here, a macrophage infection model was used to unravel the role of the histone deacetylase sirtuin 6 (SIRT6) in Mtb‐triggered regulation of the innate immune response. Mtb infection downregulated microRNA‐26a and upregulated its target SIRT6. SIRT6 suppressed glycolysis and expression of HIF‐1α‐dependent glycolytic genes during infection. In addition, SIRT6 regulated the levels of intracellular succinate which controls stabilization of HIF‐1α, as well as the release of interleukin (IL)‐1β. Furthermore, SIRT6 inhibited inducible nitric oxide synthase (iNOS) and proinflammatory IL‐6 but augmented anti‐inflammatory arginase expression. The miR‐26a/SIRT6/HIF‐1α axis therefore regulates glycolysis and macrophage immune responses during Mtb infection. Our findings link SIRT6 to rewiring of macrophage signaling pathways facilitating dampening of the antibacterial immune response. [ABSTRACT FROM AUTHOR]

Peptidyl prolyl isomerases (PPIases) are well‐conserved protein‐folding enzymes that moonlight as regulators of bacterial virulence. Peptidyl prolyl isomerase A, PPiA (Rv0009) is a secretory protein of Mycobacterium tuberculosis that possesses sequence and structural similarity to eukaryotic cyclophilins. In this study, we validated the interaction of PPiA with stimulator of interferon genes (STING) using both, Escherichia coli‐based and mammalian in vitro expression systems. In vitro pull‐down assays confirmed that the cytosolic domain of STING interacts with PPiA, and moreover, we found that PPiA could induce dimerization of STING in macrophages. In silico docking analyses suggested that the PXXP (PDP) motif of PPiA is crucial for interaction with STING, and concordantly, mutations in the PDP domain (PPiA MUT‐II) abrogated this interaction, as well as the ability of PPiA to facilitate STING dimerization. In agreement with these observations, fluorescence microscopy demonstrated that STING and wild‐type PPiA, but not PPiA MUT‐II, could colocalize when expressed in HEK293 cells. Highlighting the importance of the PDP domain further, PPiA, but not PPiA MUT‐II could activate Tank binding kinase 1 (TBK1)‐interferon regulatory factor 3 (IRF3) signaling to promote the release of interferon‐beta (IFNβ). PPiA, but not PPiA MUT‐II expressed in Mycobacterium smegmatis induced IFNβ release and facilitated bacterial survival in macrophages in a STING‐dependent manner. The PPiA‐induced release of IFNβ was c‐GAS independent. We conclude that PPiA is a previously undescribed mycobacterial regulator of STING‐dependent type I interferon production from macrophages. [ABSTRACT FROM AUTHOR]

In India, with the change of political leadership at the center, the sanctity of religion-based Muslim family law has been contested. This has led to the development of a new socio-political discourse which is influenced and shaped by the basic feminist ideals of equal rights for women. In this discourse, Muslim women are portrayed as necessarily suffering from unjust family laws and needing immediate cover and protection from the secular state. In the light of the judicial reform which makes the practice of instant divorce through 'triple talaq' among Muslims a punishable offence, this article discusses that for Muslim women the domain of law is liminal and they choose between multiple legal forums to increase their access to justice. It explores how Muslim women approach different alternative forums, and in what ways Muslim women activists are creating an opportunity for the distressed women to resolve their marital disputes more efficiently. Such an examination provides important insights into how Muslim women's rights activists undertake their pursuit of justice within a complex, legally pluralistic landscape in the area of Muslim family law in India. Keywords: Muslim women activists, Triple talaq, Judicial reforms, Muslim personal law, Legal pluralism, Islamic feminism, Uniform civil code, Introduction In civil affairs in India the main religious communities--such as Hindu, Muslim, Christian and Parsi--are governed by their respective religion based personal laws. (3) These religiously grounded personal laws [...]

Industrial organisations must now develop and implement novel approaches to operations and production if they are to compete effectively on a global scale. Changes in product development practises and the use of new technologies have occurred. Businesses encounter problems and difficulties as a direct result of the rapid pace of market change. Organisational priorities focus on minimising waste while simultaneously improving quality and cutting costs. Lean manufacturing is an established approach that should be included into every strategic company strategy. Lean manufacturing concept is a revolutionary that aims to increase resource use while minimising waste. Lean manufacturing has been shown to increase plant efficiency and decrease processing times in major manufacturing operations. In the end, industries depend greatly on their capacity to systematically and consistently cope with obstacles in order to increase product value. Therefore, a value-adding framework must be implemented; the adoption of a lean manufacturing system becomes crucial for any kind of business if it is to achieve this level of perfection. This article gives a overview of lean manufacturing their concepts, tools with application and artificial intelligence in lean manufacturing.