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Objective: Magnetic resonance imaging allows the noninvasive observation of PO2 changes between air breathing and oxygen breathing through quantification of the magnetic longitudinal relaxation time T1. Changes in PO2 are proportional to changes in the longitudinal relaxation rate ΔR1 (where ΔR1=1/T1oxygen-1/T1air). Knowledge of this response could inform clinical interventions using maternal oxygen administration antenatally to treat fetal growth restriction. We present in vivo measurements of the response of the fetal-placental unit to maternal hyperoxia.Design: Prospective cohort.Setting: Large tertiary maternity hospital.Sample: Nine women undergoing low-risk pregnancy (21-33 weeks of gestation) and five nonpregnant adults.Methods: During imaging the air supply to mothers was changed from medical air (21% oxygen) to medical oxygen (100% oxygen) and T1 was monitored over time in both the placenta and fetal brain using a periodically repeated magnetic resonance imaging sequence. To demonstrate that the method could detect a brain response, brain responses from five normal adult volunteers were measured using a similar imaging protocol.Main Outcome Measure: Changes in T1 following oxygen challenge.Results: No significant ΔR1 (P=0.42, paired t-test) was observed in fetal brains. A significant placental ΔR1 (P=0.0002, paired t-test) of 0.02±0.01/s (mean±SD) was simultaneously observed in the same participants. In the brains of the nonpregnant adults, a significant ΔR1 (P=0.01, paired t-test) of 0.005±0.002/s was observed.Conclusion: Short-term maternal oxygen administration does not improve fetal brain oxygenation, in contrast to the response observed in the adult brain. [ABSTRACT FROM AUTHOR]

Objective Magnetic resonance imaging allows the noninvasive observation of Po2 changes between air breathing and oxygen breathing through quantification of the magnetic longitudinal relaxation time T1. Changes in Po2 are proportional to changes in the longitudinal relaxation rate Δ R1 (where Δ R1 = 1/ T1oxygen − 1/ T1air). Knowledge of this response could inform clinical interventions using maternal oxygen administration antenatally to treat fetal growth restriction. We present in vivo measurements of the response of the fetal-placental unit to maternal hyperoxia. Design Prospective cohort. Setting Large tertiary maternity hospital. Sample Nine women undergoing low-risk pregnancy (21-33 weeks of gestation) and five nonpregnant adults. Methods During imaging the air supply to mothers was changed from medical air (21% oxygen) to medical oxygen (100% oxygen) and T1 was monitored over time in both the placenta and fetal brain using a periodically repeated magnetic resonance imaging sequence. To demonstrate that the method could detect a brain response, brain responses from five normal adult volunteers were measured using a similar imaging protocol. Main outcome measure Changes in T1 following oxygen challenge. Results No significant Δ R1 ( P = 0.42, paired t-test) was observed in fetal brains. A significant placental Δ R1 ( P = 0.0002, paired t-test) of 0.02 ± 0.01/s (mean ± SD) was simultaneously observed in the same participants. In the brains of the nonpregnant adults, a significant Δ R1 ( P = 0.01, paired t-test) of 0.005 ± 0.002/s was observed. Conclusion Short-term maternal oxygen administration does not improve fetal brain oxygenation, in contrast to the response observed in the adult brain. [ABSTRACT FROM AUTHOR]

Investigates the expression of the Kir2.1 gene in human placenta throughout pregnancy and in cytotrophoblast cells at different stages of differentiation in culture. Detection of the gene in all RNA samples from cytotrophoblast cells and from placentas; Data showing that the gene is expressed by the human placenta and by cytotrophoblast cells at all stages of development and differentiation.