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Accurate detection of chilling injury in kiwifruit is challenging because the symptoms are mainly manifested in the interior. This work reports a method for detecting the chilling injury of 'Hongyang' kiwifruit to provide nondestructive discrimination. Kiwifruit samples with varying levels of chilling injury were analyzed by a hyperspectral structuredillumination reflectance imaging (SIRI) system. After demodulation, direct current (DC) and alternating current (AC) images with spatial frequencies of 30, 60, and 120m-1 were obtained and labeled as F30, F60, and F120. Predictive models were developed to optimize the preprocessing and modeling methods. Prediction models established the results of DC and AC with different spatial frequencies and were compared. The autoscale-support vector machine (SVM) models were optimal for AC at different spatial frequencies, and the multiplicative scatter correction (MSC)-SVM model was optimal for DC. The combined features of F30, F60, and F120, as well as the spectral features of DC, had better accuracy for classifying chilling injury. The optimal model of hyperspectral SIRI system for detecting chilling injury was the F30 based on combined features, with calibration accuracy of 98.1% and prediction accuracy of 94.2%. This study has shown that structured illumination had higher accuracy than uniform illumination in predicting chilling injury. Further, this approach allows the identification of kiwifruit with chilling injury using a hyperspectral structured-illumination reflectance imaging system. [ABSTRACT FROM AUTHOR]