1. Antigen levels of the urokinase-type plasminogen activator and its gene polymorphisms in colorectal cancer.
- Author
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Przybyłowska, K, Smolarczyk, K, Kulig, A, Romanowicz-Makowska, H, Dziki, A, Ulańska, J, Pander, B, and Błasiak, J
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ANTIGEN analysis , *FIBRINOLYTIC agents , *COLON tumors , *COMPARATIVE studies , *GENES , *GENETIC polymorphisms , *RESEARCH methodology , *MEDICAL cooperation , *RESEARCH , *EVALUATION research , *GENOTYPES ,RECTUM tumors - Abstract
We analysed the distribution of genotypes of two polymorphisms in the urokinase-type plasminogen activator (uPA) gene: C-->T substitution in exon 6 and T-->C substitution in intron 7 in 52 subjects with colorectal cancer. Genotypes were determined in tumour tissue and distant mucosa samples by allele-specific polymerase chain reaction. The antigen levels of uPA in cancer tissue were higher than in distant mucosa as measured by enzyme-linked immunosorbent assay. The level of uPA antigens in cancer samples with the C/C genotype of C-->T polymorphism in exon 6 was higher than in samples with C/T and T/T genotypes. No differences in the level of uPA antigens between the alleles of the intron 7 T-->C polymorphism were found. As uPA can be involved in cancer invasion and metastasis, C/C genotype in exon 6 of uPA gene can be further considered as being related to colorectal cancer progression. [ABSTRACT FROM AUTHOR]
- Published
- 2002
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