Alejandra de Moreno de LeBlanc, Anderson Miyoshi, Philippe Langella, Fernando Sesma, Luis G. Bermúdez-Humarán, Laurie Watterlot, Silvina del Carmen, Jean Guy LeBlanc, Vasco Azevedo, Gabriela Perdigón, Centro de Referencia para Lactobacilos [Tucumán] (CERELA), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Inst Microbiol, Federal University of Minas Gerais, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Inst Microbiol, Fac Bioquim Quim & Farm, Catedra Inmunol, Univ Nacl Tucuman, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT), Consejo de Investigaciones de la Universidad Nacional de Tucuman (CIUNT), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), and Centro Argentino Brasileno de Biotecnologia
Reactive oxygen species are involved in various aspects of intestinal inflammation and tumor development. Decreasing their levels using antioxidant enzymes, such as catalase (CAT) or superoxide dismutase (SOD) could therefore be useful in the prevention of certain diseases. Lactic acid bacteria (LAB) are ideal candidates to deliver these enzymes in the gut. In this study, the anti-inflammatory effects of CAT or SOD producing Lwere evaluated using a trinitrobenzenesulfonic acid (TNBS) induced Crohn's disease murine model. Engineered Lactobacillus casei BL23 strains producing either CAT or SOD, or the native strain were given to mice before and after intrarectal administration of TNBS. Animal survival, live weight, intestinal morphology and histology, enzymatic activities, microbial translocation to the liver and cytokines released in the intestinal fluid were evaluated. The mice that received CAT or SOD-producing Lshowed a faster recovery of initial weight loss, increased enzymatic activities in the gut and lesser extent of intestinal inflammation compared to animals that received the wild-type strain or those that did not receive bacterial supplementation. Our findings suggest that genetically engineered Lthat produce antioxidant enzymes could be used to prevent or decrease the severity of certain intestinal pathologies. (C) 2010 Elsevier B.V. All rights reserved.