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323 results on '"VICKERS, CLAUDIA E."'

7. LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae

Author

Lu, Zeyu, primary, Shen, Qianyi, additional, Bandari, Naga Chandra, additional, Evans, Samuel, additional, McDonnell, Liam, additional, Liu, Lian, additional, Jin, Wanli, additional, Luna-Flores, Carlos Horacio, additional, Collier, Thomas, additional, Talbo, Gert, additional, McCubbin, Tim, additional, Esquirol, Lygie, additional, Myers, Chris, additional, Trau, Matt, additional, Dumsday, Geoff, additional, Speight, Robert, additional, Howard, Christopher B, additional, Vickers, Claudia E, additional, and Peng, Bingyin, additional

Published
2024
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12. Author Correction: Building a global alliance of biofoundries.

Author

Hillson, Nathan, Caddick, Mark, Cai, Yizhi, Carrasco, Jose A, Chang, Matthew Wook, Curach, Natalie C, Bell, David J, Feuvre, Rosalind Le, Friedman, Douglas C, Fu, Xiongfei, Gold, Nicholas D, Herrgård, Markus J, Holowko, Maciej B, Johnson, James R, Johnson, Richard A, Keasling, Jay D, Kitney, Richard I, Kondo, Akihiko, Liu, Chenli, Martin, Vincent JJ, Menolascina, Filippo, Ogino, Chiaki, Patron, Nicola J, Pavan, Marilene, Poh, Chueh Loo, Pretorius, Isak S, Rosser, Susan J, Scrutton, Nigel S, Storch, Marko, Tekotte, Hille, Travnik, Evelyn, Vickers, Claudia E, Yew, Wen Shan, Yuan, Yingjin, Zhao, Huimin, and Freemont, Paul S

Subjects
MD Multidisciplinary
Abstract

The original version of this Comment contained errors in the legend of Figure 2, in which the locations of the fifteenth and sixteenth GBA members were incorrectly given as '(15) Australian Genome Foundry, Macquarie University; (16) Australian Foundry for Advanced Biomanufacturing, University of Queensland.'. The correct version replaces this with '(15) Australian Foundry for Advanced Biomanufacturing (AusFAB), University of Queensland and (16) Australian Genome Foundry, Macquarie University'. This has been corrected in both the PDF and HTML versions of the Comment.

Published
2019

13. Building a global alliance of biofoundries.

Author

Hillson, Nathan, Caddick, Mark, Cai, Yizhi, Carrasco, Jose A, Chang, Matthew Wook, Curach, Natalie C, Bell, David J, Le Feuvre, Rosalind, Friedman, Douglas C, Fu, Xiongfei, Gold, Nicholas D, Herrgård, Markus J, Holowko, Maciej B, Johnson, James R, Johnson, Richard A, Keasling, Jay D, Kitney, Richard I, Kondo, Akihiko, Liu, Chenli, Martin, Vincent JJ, Menolascina, Filippo, Ogino, Chiaki, Patron, Nicola J, Pavan, Marilene, Poh, Chueh Loo, Pretorius, Isak S, Rosser, Susan J, Scrutton, Nigel S, Storch, Marko, Tekotte, Hille, Travnik, Evelyn, Vickers, Claudia E, Yew, Wen Shan, Yuan, Yingjin, Zhao, Huimin, and Freemont, Paul S

Subjects
Organisms, Genetically Modified, Genetic Engineering, Biotechnology, Biomedical Research, International Cooperation, Organisms, Genetically Modified, MD Multidisciplinary
Abstract

Biofoundries provide an integrated infrastructure to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications and research. Many biofoundries are being built and a Global Biofoundry Alliance has recently been established to coordinate activities worldwide.

Published
2019

14. Toward industrial production of isoprenoids in Escherichia coli: Lessons learned from CRISPR‐Cas9 based optimization of a chromosomally integrated mevalonate pathway

Author

Alonso‐Gutierrez, Jorge, Koma, Daisuke, Hu, Qijun, Yang, Yuchen, Chan, Leanne JG, Petzold, Christopher J, Adams, Paul D, Vickers, Claudia E, Nielsen, Lars K, Keasling, Jay D, and Lee, Taek S

Subjects
Biological Sciences, Industrial Biotechnology, Genetics, Biofuels, CRISPR-Cas Systems, Chromosomes, Bacterial, Escherichia coli, Genome, Bacterial, Industrial Microbiology, Metabolic Engineering, Mevalonic Acid, Microorganisms, Genetically-Modified, Operon, Sesquiterpenes, Sucrose, biofuel, chromosomal engineering, CRISPR-Cas9, E. coli DH1, mevalonate pathway, sucrose utilization, Biotechnology
Abstract

Escherichia coli has been the organism of choice for the production of different chemicals by engineering native and heterologous pathways. In the present study, we simultaneously address some of the main issues associated with E. coli as an industrial platform for isoprenoids, including an inability to grow on sucrose, a lack of endogenous control over toxic mevalonate (MVA) pathway intermediates, and the limited pathway engineering into the chromosome. As a proof of concept, we generated an E. coli DH1 strain able to produce the isoprenoid bisabolene from sucrose by integrating the cscAKB operon into the chromosome and by expressing a heterologous MVA pathway under stress-responsive control. Production levels dropped dramatically relative to plasmid-mediated expression when the entire pathway was integrated into the chromosome. In order to optimize the chromosomally integrated MVA pathway, we established a CRISPR-Cas9 system to rapidly and systematically replace promoter sequences. This strategy led to higher pathway expression and a fivefold improvement in bisabolene production. More interestingly, we analyzed proteomics data sets to understand and address some of the challenges associated with metabolic engineering of the chromosomally integrated pathway. This report shows that integrating plasmid-optimized operons into the genome and making them work optimally is not a straightforward task and any poor engineering choices on the chromosome may lead to cell death rather than just resulting in low titers. Based on these results, we also propose directions for chromosomal metabolic engineering.

Published
2018

16. The integration of tandem gene repeats via a bacterial type-II toxin-antitoxin-mediated gene amplification (ToxAmp) system and stability visualisation in Saccharomyces cerevisiae

Author

Evans, Samuel, primary, Lu, Zeyu, additional, McDonnell, Liam, additional, Anderson, Will, additional, Peralta, Francisco, additional, Watkins, Tyson, additional, Ahmed, Hafna, additional, Luna-Flores, Carlos Horacio, additional, Loan, Thomas, additional, Navone, Laura, additional, Trau, Matt, additional, Scott, Colin, additional, Speight, Robert, additional, Vickers, Claudia E., additional, and Peng, Bingyin, additional

Published
2024
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17. Cyanamide-Inducible Expression of Homing Nuclease I-Scei for Iterative Genome Engineering and Parallel Promoter Characterisation in Saccharomyces Cerevisiae

Author

McDonnell, Liam, primary, Evans, Samuel, additional, Lu, Zeyu, additional, Suchoronczak, Mitch, additional, Leighton, Jonah, additional, Ordeniza, Eugene, additional, Ritchie, Blake, additional, Valado, Nik, additional, Walsh, Niamh, additional, Antoney, James, additional, Wang, Chengqiang, additional, Luna-Flores, Carlos Horacio, additional, Scott, Colin, additional, Speight, Robert, additional, Vickers, Claudia E., additional, and Peng, Bingyin, additional

Published
2024
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18. Integration of Yeast Episomal/Integrative Plasmid Causes Genotypic and Phenotypic Diversity and Improved Sesquiterpene Production in Metabolically Engineered Saccharomyces cerevisiae

Author

Peng, Bingyin, primary, Weintraub, Sarah J., additional, Lu, Zeyu, additional, Evans, Samuel, additional, Shen, Qianyi, additional, McDonnell, Liam, additional, Plan, Manuel, additional, Collier, Thomas, additional, Cheah, Li Chen, additional, Ji, Lei, additional, Howard, Christopher B., additional, Anderson, Will, additional, Trau, Matt, additional, Dumsday, Geoff, additional, Bredeweg, Erin L., additional, Young, Eric M., additional, Speight, Robert, additional, and Vickers, Claudia E., additional

Published
2023
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19. Formation of Isoprenoids

Author

Pérez-Gil, Jordi, Rodríguez-Concepción, Manuel, Vickers, Claudia E., Timmis, Kenneth N., Editor-in-Chief, Boll, Matthias, Series Editor, Geiger, Otto, Series Editor, Goldfine, Howard, Series Editor, Krell, Tino, Series Editor, Lee, Sang Yup, Series Editor, McGenity, Terry J., Series Editor, Rojo, Fernando, Series Editor, Sousa, Diana Z, Series Editor, Stams, Alfons J. M., Series Editor, Steffan, Robert, Series Editor, and Wilkes, Heinz, Series Editor

Published
2019
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23. Dynamic Balancing of Isoprene Carbon Sources Reflects Photosynthetic and Photorespiratory Responses to Temperature Stress

Author

Jardine, Kolby, Chambers, Jeffrey, Alves, Eliane G, Teixeira, Andrea, Garcia, Sabrina, Holm, Jennifer, Higuchi, Niro, Manzi, Antonio, Abrell, Leif, Fuentes, Jose D, Nielsen, Lars K, Torn, Margaret S, and Vickers, Claudia E

Subjects
Plant Biology, Biological Sciences, Butadienes, Carbon, Carbon Dioxide, Carbon Isotopes, Environment, Hemiterpenes, Hot Temperature, Light, Pentanes, Photosynthesis, Plant Leaves, Temperature, Terpenes, Trees, Agricultural and Veterinary Sciences, Plant Biology & Botany, Plant biology
Abstract

The volatile gas isoprene is emitted in teragrams per annum quantities from the terrestrial biosphere and exerts a large effect on atmospheric chemistry. Isoprene is made primarily from recently fixed photosynthate; however, alternate carbon sources play an important role, particularly when photosynthate is limiting. We examined the relative contribution of these alternate carbon sources under changes in light and temperature, the two environmental conditions that have the strongest influence over isoprene emission. Using a novel real-time analytical approach that allowed us to examine dynamic changes in carbon sources, we observed that relative contributions do not change as a function of light intensity. We found that the classical uncoupling of isoprene emission from net photosynthesis at elevated leaf temperatures is associated with an increased contribution of alternate carbon. We also observed a rapid compensatory response where alternate carbon sources compensated for transient decreases in recently fixed carbon during thermal ramping, thereby maintaining overall increases in isoprene production rates at high temperatures. Photorespiration is known to contribute to the decline in net photosynthesis at high leaf temperatures. A reduction in the temperature at which the contribution of alternate carbon sources increased was observed under photorespiratory conditions, while photosynthetic conditions increased this temperature. Feeding [2-(13)C]glycine (a photorespiratory intermediate) stimulated emissions of [(13)C1-5]isoprene and (13)CO2, supporting the possibility that photorespiration can provide an alternate source of carbon for isoprene synthesis. Our observations have important implications for establishing improved mechanistic predictions of isoprene emissions and primary carbon metabolism, particularly under the predicted increases in future global temperatures.

Published
2014

24. Integration of Yeast Episomal/Integrative Plasmid Causes Genotypic and Phenotypic Diversity and Improved Sesquiterpene Production in Metabolically Engineered Saccharomyces cerevisiae.

Author

Peng, Bingyin, Weintraub, Sarah J., Lu, Zeyu, Evans, Samuel, Shen, Qianyi, McDonnell, Liam, Plan, Manuel, Collier, Thomas, Cheah, Li Chen, Ji, Lei, Howard, Christopher B., Anderson, Will, Trau, Matt, Dumsday, Geoff, Bredeweg, Erin L., Young, Eric M., Speight, Robert, and Vickers, Claudia E.

Published
2024
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30. Isoprene

Author

Vickers, Claudia E., Sabri, Suriana, Scheper, Thomas, Series editor, Belkin, Shimshon, Series editor, Doran, Pauline M., Series editor, Gu, Man Bock, Series editor, Hu, Wei-Shou, Series editor, Mattiasson, Bo, Series editor, Nielsen, Jens, Series editor, Seitz, Harald, Series editor, Stephanopoulos, Gregory N., Series editor, Ulber, Roland, Series editor, Zeng, An-Ping, Series editor, Zhong, Jian-Jiang, Series editor, Zhou, Weichang, Series editor, Schrader, Jens, editor, and Bohlmann, Jörg, editor

Published
2015
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35. Ancestral sequence reconstruction of the CYP711 family reveals functional divergence in strigolactone biosynthetic enzymes associated with gene duplication events in monocot grasses

Author

Vinde, Marcos H., primary, Cao, Da, additional, Chesterfield, Rebecca J., additional, Yoneyama, Kaori, additional, Gumulya, Yosephine, additional, Thomson, Raine E. S., additional, Matila, Tebogo, additional, Ebert, Birgitta E., additional, Beveridge, Christine A., additional, Vickers, Claudia E., additional, and Gillam, Elizabeth M. J., additional

Published
2022
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37. Analysing intracellular isoprenoid metabolites in diverse prokaryotic and eukaryotic microbes

Author

Plan, Manuel, Bongers, Mareike, Bydder, Sarah, Fabris, Michele, Hodson, Mark P., Kelly, Erin, Krömer, Jens, Perez-Gil, Jordi, Peng, Bingyin, Satta, Alessandro, Schrübbers, Lars, and Vickers, Claudia E.

Subjects
Intracellular metabolites, Synechocystis sp. PCC 6803, Mevalonate pathway, Terpenoids, Escherichia coli, Prenyl phosphate metabolism, Saccharomyces cerevisiae, Isoprenoids, Phaeodactylum tricornutum, Chlamydomonas reinhardtii, Methylerythritol phosphate pathway
Abstract

Chapter 8.-- 50 pages.-- Edited by Eleanore T. Wurtzel., Isoprenoids, also known as terpenes or terpenoids, are a very large and diverse group of natural compounds. These compounds fulfil a myriad of critical roles in biology as well as having a wide range of industrial uses. Isoprenoids are produced via two chemically distinct metabolic pathways, the mevalonate (MVA) pathway and the methylerythritol phosphate (MEP) pathway. Downstream of these two pathways is the shared prenyl phosphate pathway. Because of their importance in both basic physiology and industrial biotechnology, extraction, identification, and quantification of isoprenoid pathway intermediates is an important protocol. Here we describe methods for extraction and analysis of intracellular metabolites from the MVA, MEP, and prenyl phosphate pathways for five key model microbes: the yeast Saccharomyces cerevisiae, the bacterium Escherichia coli, the diatom Phaeodactylum tricornutum, the green algae Chlamydomonas reinhardtii, and the cyanobacterium Synechocystis sp. PCC 6803. These methods also detect several central carbon intermediates. These protocols will likely work effectively, or be readily adaptable, to a variety of related microorganisms and metabolic pathways.

Published
2022

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