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Systematic inflammatory response syndrome (SIRS) and the accompanying sepsis pose a huge threat to human health worldwide. Heparin is a part of the standard supportive care for the disease. However, the molecular mechanism is not fully understood yet, and the potential signaling pathways that play key roles have not yet been elucidated. In this paper, the main findings regarding the molecular mechanisms associated with the beneficial effects of heparin, including inhibiting HMGB-1-driven inflammation reactions, histone-induced toxicity, thrombo-inflammatory response control and the new emerging mechanisms are concluded. To set up the link between the preclinical research and the clinical effects, the outcomes of the clinical trials are summarized. Then, the structure and function relationship of heparin is discussed. By providing an updated analysis of the above results, the paper highlights the feasibility of heparin as a possible alternative for sepsis prophylaxis and therapy. [ABSTRACT FROM AUTHOR]

Background: Cough is one of the most common symptoms in the post-COVID syndrome, often occurring alongside other symptoms such as back pain, fatigue, and dyspnea. Case presentation: A 60-yearold female experienced chronic cough and back pain after recovering from COVID-19. She underwent 3 sessions of cupping and acupuncture therapy and made a full recovery. Discussions: We have found that cupping therapy is an effective treatment for post-COVID cough. The appearance of blisters during cupping is a normal occurrence and may even enhance the therapeutic effects. Therefore, it should not be considered malpractice. [ABSTRACT FROM AUTHOR]

The objectives of this study were to characterize dietary fiber (DF) intake in patients with ankylosing spondylitis (AS), to assess whether DF intake affects disease activity in AS, and to investigate the effect of DF intake on disease activity in AS in the context of functional bowel disease (FBD) symptoms. We recruited 165 patients with AS and divided them into two groups according to whether they had a high DF intake > 25 g/d to investigate the characteristics of people with high DF intake. Some 72 of the 165 AS patients (43%) met the criteria for high DF intake, which was more common in patients with negative FBD symptoms (68%). Data analysis revealed that DF intake was negatively associated with AS disease activity and did not differ statistically significantly from FBD symptoms. Multivariate adjusted models were used to explore the effect of DF intake on AS disease activity. ASDAS-CRP and BASDAI were stable and negatively correlated across models in both groups with and without FBD symptoms. Thus, DF intake positively affected disease activity in patients with AS. ASDAS-CRP and BASDAI were negatively correlated with DF intake. [ABSTRACT FROM AUTHOR]

Alginate hydrogel has received great attention in diabetic wound healing. However, the limited tunability of the ionic crosslinking method prevents the delicate management of physical properties in response to diverse wound conditions. We addressed this issue by using a microgel particle (fabricated by zinc ions and coordinated through the complex of carboxymethyl chitosan and aldehyde hyaluronic acid) as a novel crosslinker. Then the cation was introduced as a second crosslinker to create a double crosslinked network. The method leads to the precise regulation of the hydrogel characters, including the biodegradation rate and the controlled release rate of the drug. As a result, the optimized hydrogels facilitated the live-cell infiltration in vitro and boosted the tissue regeneration of diabetic wounds in vivo. The results indicated that the addition of the microgel as a new crosslinker created flexibility during the construction of the alginate hydrogel, adapting for diverse applications during diabetic-induced wound therapy. [ABSTRACT FROM AUTHOR]

Heparin has shown benefits in severe acute pancreatitis (SAP) therapy, but the underlying mechanisms were unknown. Extracellular high-mobility group protein-1 (HMGB-1) has been regarded as a central mediator contributing to inflammation exacerbation and disease aggravation. We hypothesized heparin attenuated the disease by targeting HMGB-1-related pathways. In the present study, the possible therapeutic roles of heparin and its non-anticoagulant derivatives, 6-O-desulfulted heparin and N-acylated-heparin, were determined on mouse models induced by "Two-Hit" of L-arginine. The compounds exhibited potent efficiency by substantially decreasing the pancreatic necrosis, macrophage infiltration, and serum inflammatory cytokine (IL-6 and TNF-α) concentration. Moreover, they greatly reduced the rapidly increasing extracellular HMGB-1 levels in the L-arginine injured pancreases. As a result, multiple organ failure and mortality of the mice were inhibited. Furthermore, the drugs were incubated with the RAW264.7 cells activated with damaged pancreatic tissue of SAP mice in vitro. They were found to inhibit HMGB-1 transfer from the nucleus to the plasma, a critical step during HMGB-1 active secretion from macrophages. The results were carefully re-examined with a caerulein and LPS induced mouse model, and similar results were found. The paper demonstrated heparin alleviated SAP independent of the anti-coagulant functions. Therefore, non-anticoagulant heparin derivatives might become promising approaches to treat patients suffering from SAP. [ABSTRACT FROM AUTHOR]

Many workers who engage in manual material handling (MMH) jobs experience high physical demands that are associated with work-related musculoskeletal disorders (WMSDs). Quantifying the physical demands of a job is important for identifying high risk jobs and is a legal requirement in the United States for hiring and return to work following injury. Currently, most physical demand analyses (PDAs) are performed by experts using observational and semi-quantitative methods. The lack of accuracy and reliability of these methods can be problematic, particularly when identifying restrictions during the return-to-work process. Further, when a worker does return-to-work on modified duty, there is no way to track compliance to work restrictions conflating the effectiveness of the work restrictions versus adherence to them. To address this, we applied a deep learning model to data from eight inertial measurement units (IMUs) to predict 15 occupational physical activities. Overall, a 95% accuracy was reached for predicting isolated occupational physical activities. However, when applied to more complex tasks that combined occupational physical activities (OPAs), accuracy varied widely (0–95%). More work is needed to accurately predict OPAs when combined into simulated work tasks. [ABSTRACT FROM AUTHOR]

Numerous ear-related wearables require precise measurements of the external acoustic meatus (EAM) to optimise function and comfort. The purpose of this study is to describe a novel methodology for measuring the EAM. A total of 23 measurement variables (18 novel) of the EAM from the entrance to the second bend were collected on 700 Chinese subjects (age: 15–83) using casting and 3D scanning over seven age spans: 10, 20, 30, 40, 50, 60 s and 70+. The ear horizontal plane was identified as a new reference plane for measurements and the medial concha was selected as the reference point for positioning the entrance. A detailed approach to characterising the EAM was developed as was an approach for the rapid estimation of circumference and area using regression equations making it ideal for use in early design conceptualizations. Practitioner summary: This study provides a scalable measurement methodology for determining anthropometric measurements of the external acoustic meatus. The measurement methodology and its application to the design and fitting of ear-related wearables are important to optimising their function and comfort. [ABSTRACT FROM AUTHOR]

This paper proposes the use of digital knitting to fabricate three-dimensional stretchable fabric with transformative properties. The research focus is on the application of the curling effect and the resultant stitch structures. Rib, purl, and links structures, which have alternating face and back loops in the structural knit cell and produced the curling effect, are investigated. Thirty-six samples based on the three structures along with 12 different stitch combinations are produced through the digital knitting process. The properties of the samples, including the dimensional changes, surface texture, weight and thickness, and tensile properties, are subsequently evaluated and compared by using both quantitative and qualitative assessment methods. The results show that the developed knitted fabrics that use the curling effect through alternating face and back loops are significantly three-dimensional in surface texture and have considerable stretchability. These effects in general increase with the growth of stitch number in a structural knit cell. However, only the links structures show considerable extensibility along both course and wale directions, while the rib and purl structures have enhanced stretchability in only the course and wale directions, respectively. Therefore, this design-led textile study provides a simple but effective means for producing new materials that offer both function and aesthetics for fashion with transformable designs. The work here also provides a technical-based integrated approach for innovative textile and fashion developments. [ABSTRACT FROM AUTHOR]

The development of DNA assays based on gold nanoparticles (AuNPs) holds great promise for point‐of‐care diagnosis at molecular level; nevertheless, how to accelerate the hybridization reaction rates, and design rapid DNA assay in aqueous solution remains challenging. Herein, a pH switchable AuNP aggregation system, functionalized with compound Y1, was set up. This method provided an elegant strategy for the precise control of DNA hybridization reaction kinetics when Y1 was co‐functionalized with ssDNA on the AuNPs. Using this strategy, real‐time DNA detection was realized by simply adjusting the pH value of the solution without affecting the sensitivity. Particularly worth mentioning, this system maintained high specificity, and were capable of accurately discriminating against single‐based mismatched sequences. In total, this system significantly improved the rapidity and robustness of ssDNA modified AuNPs, providing sound basis for the low‐cost, and disposable molecular diagnosis design. We demonstrated that Y1 modified AuNP triggered pH reversible aggregation behaviour in aqueous solution because of the varied hydrogen bonding strength derived from carboxylic acid. In this study, Y1‐ssDNA co‐functionalized AuNP, instead of ssDNA modified AuNP, was selected for DNA detection. Using this system, the target DNA the detection time can be shortened to within 1 min by simply adjusting the solution pH. [ABSTRACT FROM AUTHOR]

[Display omitted] • The effects of crosslinking and branching degree on phenolic pyrolysis were studied. • The bimodal distribution of pyrolysis products was obtained. • The generation activation energy of low-boiling molecules was calculated. • The reaction kinetics path of phenolic pyrolysis was reasonably summarized. Based on the reactive force-field (ReaxFF) molecular dynamics, the thermostability of cross-linked and non-cross-linked phenolic was compared to study the effect of cross-linked and branched structures on the pyrolysis mechanism. The results show that during the pyrolysis process, phenolic tends to break into low-boiling molecules with less than 4 carbon atoms and polymerizes into macromolecules with more than 1500 carbon atoms. The results of residual carbon rate and products distribution confirm that carbon skeleton of phenolic resin with high degree of crosslinking and branching is better preserved and has better thermostability. The reaction kinetics path of phenolic pyrolysis was reasonably summarized, revealing the generation mechanism of low-boiling molecules with less than 4 carbon atoms and large carbon clusters, which is in good agreement with the experimental results of activation energy and chemical bond energy. The reaction kinetics data obtained in this work provide a reliable basis for multi-scale reaction simulation. [ABSTRACT FROM AUTHOR]

[Display omitted] • Two fractions (A and C) of GAGs from porcine jejunum and duodenum were purified. • A is a heparan sulfate, and C is composed of β- d -GlcA-(1 → 4) and β- d -GalNAc-(1 → 3). • They possessed controllable anti-coagulant activity in vitro. • Additional sulfate modification enhanced the effect. To make full use of the porcine sources and develop better choice of novel GAGs as anti-coagulants, two fractions of GAGs from the porcine jejunum (A) and duodenum (C) have been separated & purified. The products were further sulfated to give B and D in order to test the influence of sulfate pattern on the bioactivity. The results showed that the relative molecular weight range of A was 3000–50,000 (Mw, g/mol), whereas C had an average molecular weight of 75,885 (Mw, g/mol). A was identified as a novel heparan sulfate through enzymatic hydrolysis analysis. C was a chondroitin like polysaccharide mainly composed of β - d -GlcA-(1 → 4) and β - d -GalNAc-(1 → 3). A possessed controllable anti-coagulant activity (7 IU/mg) in vitro. The activity of D almost achieved the same magnitude of A. This study demonstrated the anticoagulant potential of the polysaccharides, providing solid foundation for development of anti-coagulants from porcine intestine. [ABSTRACT FROM AUTHOR]

To develop a severe acute pancreatitis (SAP) model transited from mild symptoms, we investigated a "two-hit" strategy with L-arginine in mice. The mice were intraperitoneally injected with ice-cold L-arginine (4 g/kg) twice at an interval of 1 h on the first day and subjected to the repeated operation 72 h afterwards. The results showed the "two-hit" strategy resulted in the destructive damage and extensive necrosis of acinar cells in the pancreas compared with the "one-hit" model. Meanwhile, excessive levels of pro-inflammatory mediators, namely IL-6 and TNF-α, were released in the serum. Remarkably, additional deleterious effects on multiple organs were observed, including high intestinal permeability, kidney injury, and severe acute lung injury. Therefore, we confirmed that the SAP animal model triggered by a "two-hit" strategy with L-arginine was successfully established, providing a solid foundation for a deeper understanding of SAP initiation and therapy research to prevent worsening of the disease. [ABSTRACT FROM AUTHOR]

A DNA diagnostic system has been developed by using a bacterial reporter probe. DNA hybridization-driven magnetic isolation of E. coli, followed by exponential bacterial growth and fluorescent protein expression, allows the translation of a target binding event into a vastly amplified signal and a highly sensitive/selective detection platform. [ABSTRACT FROM AUTHOR]

Diabetic foot ulcer is one of the most serious complications of diabetes. Effective medical treatment regarding improvement of ulcer healing in patients is essential. Pien Tze Huang (PZH), a valuable Chinese traditional medicine, has been found significant efficacy on the curing of diabetic wound in clinic recently. This work was conducted to confirm the efficacy, and compare the therapeutic effect through the oral administration and local delivery route, providing a rationale for the new PZH form development; besides, the mechanisms through which PZH promoted the wound healing was also discussed. First, the chemical composition of PZH was characterized by 1H-NMR and HPLC. The anti-apoptosis effects of PZH on high concentration glucose injured epidermal fibroblast (HFF-1) was investigated in a dose dependent way. Then, the effects of the systematical administration of PZH, and the topical used route on excisional wounds of Streptozotocin (STZ) induced diabetic mice were compared. The results illustrated that PZH decreased the reactive oxygen species (ROS) levels in cells, preventing cell damage/apoptosis through an ROS/Bcl-2/Bax/Caspase-3 pathway. The in vivo study proved that topical use of PZH exceeded the systematical route both in accelerating the wound closure and improving the healing quality. Meanwhile, PZH promoted wound closure through stimulating the secretion of Col-I, decreasing fibroblast apoptosis, and enhancing myo-fibroblast differentiation, in consistent with the mechanism study in vitro. Local used PZH improves wound healing by inhibiting the abnormal HFF-1 apoptosis and senescence. The study held a great promise for development of a topical dosage form of PZH for diabetic wound healing. Image 1 • Impaired wound healing of the ulcers is the most common complications of diabetic patients. • Pien Tze Huang (PZH) effectively prevented the abnormal apoptosis and senescence of fibroblast, which is generally regarded as one of the key reasons contribute to a very poor efficacy of pharmaceutical treatment. • PZH decreased ROS generation in cell, preventing apoptosis through a ROS/Bcl-2/Bax/Caspase-3 pathway. • Topical administration of PZH promoted the chronic wound closure on diabetic mice. • The study proved PZH as a promising drug for diabetic wound healing. [ABSTRACT FROM AUTHOR]

It is widely known that the printing quality of fused filament fabrication (FFF) is heavily affected by environmental temperature and humidity, taking the form of warping and porosity. However, there is little understanding about the quantitative relations between environmental conditions, geometry, and the mechanical properties of printed parts. In this study, we systematically investigated those relations using bisphenol A polycarbonate as a model material system. For the environmental temperature, an in-situ infrared imaging analysis revealed the presence of an up to 5.4 °C/mm thermal gradient when printing using an open-chamber printer and a heated build plate. For the environmental humidity, an analysis of X-ray micro-computed tomography (micro-CT) scans showed an up to 11.7% porosity that was brought by polymer water content absorbed from environmental moisture. Meanwhile, tensile tests showed a mechanical performance loss associated with those defects, but, surprisingly, the transverse direction ductility had the potential to increase at a higher porosity. Furthermore, the experimental results were combined with analytical and parametrical studies to elucidate quantitative relations between environmental conditions and printing quality. Based on the results, quantitative guidelines for the estimation of printing quality based on environmental conditions are provided that would also help users to obtain desired printing results with a better understanding of the effects of environmental conditions. [ABSTRACT FROM AUTHOR]

The diabetic wounds do not heal easily in part because they are susceptible to infection due to environmental influences. Wound dressing is crucial to wound healing, as it can basically protect the wound from external damages and provide a suitable microenvironment for tissue regeneration. In this study, a double-layer membrane that consists of chitosan sponge and decellularized bovine amniotic membrane (dBAM) has been developed by freeze-casting method. The results showed that the porous structure of the sponge layer improved the performances of blood coagulation and swelling. The dense dBAM can optimize the mechanical property of wound dressing. In vitro studies revealed that the bilayer membrane had favorable biocompatible, especially for human foreskin fibroblast cells (HFF-1) cell adhesion and proliferation. Moreover, the full-thickness skin defects of diabetic model mice that treated with bilayer membrane showed over 80% closure in 8 days. Our findings imply that the double-layer dressing has great potentials to be used in diabetic patients. [ABSTRACT FROM AUTHOR]

• A wearable thermoelectric PPE cooling system without compromising protection. • The system can extract 51.7 W heat and 26.3 g/hour moisture within protective coverall for 6 hours. • The mean cooling power per unit weight is 3.2 ∼ 5.0 times over the commercial products. • The cooling performance is equivalent to 3.5 °C drop in ambient temperature. • Potentially save 12 % ∼ 30 % building HVAC energy consumption. While PPE prevents the health care personnel from exposing to the harmful surroundings, it creates a barrier to the dissipation of body heat and perspiration, leading to severe heat stress during prolonged exposure. Here, we report a lightweight wearable cooling and dehumidifying system capable of extracting 51.7 W heat and 26.3 g/hour moisture within protective coverall without compromising the protection. The system could continuously operate for 6 h without taking off the PPE. Compared with the available commercial cooling garments with different cooling methods, the system could provide about 3.2 ∼ 5.0 times mean cooling power per unit weight in a prolonged working condition. In a simulated hospital environment, the optimum cooling effect was equivalent to 3.5 °C drop in ambient temperature and 6 % decrease in ambient relative humidity. It will not only enhance user's comfort and performance but potentially reduce the heating, ventilation and air conditioning (HVAC) energy consumption by 12 % ∼ 30 % in building environments. [ABSTRACT FROM AUTHOR]