25 results on '"Zoran Marinović"'
Search Results
2. Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax)
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Marta Blanes-García, Zoran Marinović, Marina Morini, Alain Vergnet, Ákos Horváth, and Juan F. Asturiano
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transplantation ,surrogate production ,surrogacy ,spermatogonial stem cells ,testis ,cryopreservation ,Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
The European eel encounters challenges in achieving sexual maturation in captivity, which has been a concern for researchers. This study explores surrogate broodstock technology as an alternative approach for eel production. The present study aimed to evaluate zebrafish and European sea bass as potential recipients for European eel spermatogonia transplantation, given the abundance of eel type A spermatogonia (SPGA). Immature European eel testes were dissected and maintained at 4 °C or cryopreserved. SPGA were obtained by dissociation of fresh or post-thawed tissue, employing an enzymatic solution, and then labelled with fluorescent membrane marker PKH26. SPGA from fresh tissue were transplanted into wild-type zebrafish larvae and triploid European sea bass larvae, while SPGA from cryopreserved testis were transplanted into vasa::egfp transgenic zebrafish larvae. One-and-a-half months post-transplantation (mpt), fluorescent donor cells were not detected in the gonads of zebrafish or European sea bass. Molecular qPCR analyses at 1.5 or 6 mpt did not reveal European eel-specific gene expression in the gonads of any transplanted fish. The findings suggest that the gonadal microenvironments of zebrafish and European sea bass are unsuitable for the development of European eel spermatogonia, highlighting distinctive spermatogonial stem cell migration mechanisms within teleost species
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- 2024
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3. Evaluation of triploid rainbow trout Oncorhynchus mykiss as a surrogate parent for brown trout Salmo trutta m. fario and grayling Thymallus thymallus
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Zoran Marinović, Jelena Lujić, Simona Sušnik Bajec, Ida Djurdjevič, Aleš Snoj, György Hoitsy, Béla Urbányi, and Ákos Horváth
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Germline stem cells ,Spermatogonia ,Oogonia ,Transplantation ,Histology ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Surrogate production through germline stem cell (GSC) transplantation is becoming a very promising technology in species management and ex situ species conservation. In the present study, we have evaluated if the triploid rainbow trout is a suitable surrogate parent for the production of other salmonid gametes. In three independent trials conducted in two countries, we have transplanted brown trout and grayling GSCs into triploid rainbow trout larvae as recipients. Two months after transplantation, dissection of recipients displayed that both spermatogonial (SSCs) and oogonial (OSCs) stem cells of both species were able to colonize recipient gonads. After three years of rearing, neither male nor female recipients displayed signs of gametogenesis progression. Species-specific (for both donor and recipient species) amplification of mtDNA control region on the other hand displayed a presence of donor-derived germ cells within recipient gonads. This indicated that even though donor cells were able to colonize recipient gonads, they remained in a dormant or quasi-dormant state, and did not progress with gametogenesis. This study displays that the rainbow trout is not a suitable recipient for all salmonid species, and that careful selection of recipients is a crucial step in developing the surrogate production technology.
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- 2022
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4. Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation
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Zoran Marinović, Qian Li, Jelena Lujić, Yoshiko Iwasaki, Zsolt Csenki, Béla Urbányi, Goro Yoshizaki, and Ákos Horváth
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Medicine ,Science - Abstract
Abstract Zebrafish is one of the most commonly used model organisms in biomedical, developmental and genetic research. The production of several thousands of transgenic lines is leading to difficulties in maintaining valuable genetic resources as cryopreservation protocols for eggs and embryos are not yet developed. In this study, we utilized testis cryopreservation (through both slow-rate freezing and vitrification) and spermatogonia transplantation as effective methods for long-term storage and line reconstitution in zebrafish. During freezing, utilization of 1.3 M of dimethyl sulfoxide (Me2SO) displayed the highest spermatogonia viability (~60%), while sugar and protein supplementation had no effects. Needle-immersed vitrification also yielded high spermatogonia viability rates (~50%). Both optimal slow-rate freezing and vitrification protocols proved to be reproducible in six tested zebrafish lines after displaying viability rates of >50% in all lines. Both fresh and cryopreserved spermatogonia retained their ability to colonize the recipient gonads after intraperitoneal transplantation of vasa::egfp and actb:yfp spermatogonia into wild-type AB recipient larvae. Colonization rate was significantly higher in dnd-morpholino sterilized recipients than in non-sterilized recipients. Lastly, wild-type recipients produced donor-derived sperm and donor-derived offspring through natural spawning. The method demonstrated in this study can be used for long-term storage of valuable zebrafish genetic resources and for reconstitution of whole zebrafish lines which will greatly improve the current preservation practices.
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- 2019
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5. Gill Histopathology as a Biomarker for Discriminating Seasonal Variations in Water Quality
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Zoran Marinović, Branko Miljanović, Béla Urbányi, and Jelena Lujić
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environmental monitoring ,histopathological biomarkers ,histopathological alterations ,fish gills ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Histopathological alterations in various fish organs have a pronounced value in aquatic toxicology and are widely used in environmental monitoring. The aim of this study was to evaluate whether histopathological alterations in fish gills can discriminate seasonal variations in environmental conditions within the same aquatic ecosystem, and if so, which alterations contributed the most to seasonal differentiation. Microscopic examination of common bream Abramis brama gills displayed various alterations in gill structure, including epithelial hypertrophy, hyperplasia, mucous and chloride cell alterations, epithelial lifting, necrosis, hyperemia and aneurism. These alterations were subsequently quantified by a semi-quantitative analysis in order to detect differences in the intensity of the mentioned alterations. Epithelial hypertrophy, hyperplasia, epithelial lifting and necrosis varied significantly between seasons with only necrosis being significantly higher in the first season. Discriminant canonical analysis displayed that epithelial hyperplasia, mucous cell alterations, epithelial lifting and necrosis contributed the most to discrimination between seasons. Overall, this study demonstrates that histopathological biomarkers in fish gills can be used in discriminating seasonal variations in water quality within the same aquatic ecosystem.
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- 2021
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6. Does the Kis-Balaton Water Protection System (KBWPS) Effectively Safeguard Lake Balaton from Toxic Cyanobacterial Blooms?
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Zoran Marinović, Nada Tokodi, Damjana Drobac Backović, Ilija Šćekić, Nevena Kitanović, Snežana B. Simić, Nevena B. Đorđević, Árpád Ferincz, Ádám Staszny, Tamara Dulić, Jussi Meriluoto, Béla Urbányi, Jelena Lujić, and Zorica Svirčev
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cyanobacteria ,cyanotoxins ,microcystin ,Hungary ,histopathology ,Biology (General) ,QH301-705.5 - Abstract
Lake Balaton is the largest shallow lake in Central Europe. Its water quality is affected by its biggest inflow, the Zala River. During late 20th century, a wetland area named the Kis-Balaton Water Protection System (KBWPS) was constructed in the hopes that it would act as a filter zone and thus ameliorate the water quality of Lake Balaton. The aim of the present study was to test whether the KBWPS effectively safeguards Lake Balaton against toxic cyanobacterial blooms. During April, May, July and September 2018, severe cyanobacterial blooming was observed in the KBWPS with numbers reaching up to 13 million cells/mL at the peak of the bloom (July 2018). MC- and STX-coding genes were detected in the cyanobacterial biomass. Five out of nine tested microcystin congeners were detected at the peak of the bloom with the concentrations of MC-LR reaching 1.29 µg/L; however, accumulation of MCs was not detected in fish tissues. Histopathological analyses displayed severe hepatopancreas, kidney and gill alterations in fish obtained throughout the investigated period. In Lake Balaton, on the other hand, cyanobacterial numbers were much lower; more than 400-fold fewer cells/mL were detected during June 2018 and cyanotoxins were not detected in the water. Hepatic, kidney and gill tissue displayed few alterations and resembled the structure of control fish. We can conclude that the KBWPS acts as a significant buffering zone, thus protecting the water quality of Lake Balaton. However, as MC- and STX-coding genes in the cyanobacterial biomass were detected at both sites, regular monitoring of this valuable ecosystem for the presence of cyanobacteria and cyanotoxins is of paramount importance.
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- 2021
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7. Cryopreservation and transplantation of common carp spermatogonia.
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Roman Franěk, Zoran Marinović, Jelena Lujić, Béla Urbányi, Michaela Fučíková, Vojtěch Kašpar, Martin Pšenička, and Ákos Horváth
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Medicine ,Science - Abstract
Common carp (Cyprinus carpio) is one of the most cultured fish species over the world with many different breeds and plenty of published protocols for sperm cryopreservation. However, data regarding preservation of gonadal tissue and surrogate production is still missing. A protocol for freezing common carp spermatogonia was developed through varying different factors along a set of serial subsequent experiments. Among the six cryoprotectants tested, the best survival was achieved with dimethyl sulfoxide (Me2SO). In the next experiment, a wide range of cooling rates (0.5-10°C/min) and different concentrations of Me2SO were tested resulting in the highest survival achieved using 2 M Me2SO and cooling rate of -1°C/min. When testing different tissue sizes and incubation times in the cryomedia, the highest viability was observed when incubating 100 mg tissue fragments for 30 min. Finally, sugar supplementation did not yield significant differences. When testing different equilibration (ES) and vitrification solutions (VS) used for needle-immersed vitrification, no significant differences were observed between the tested groups. Additionally, varied exposure time to VS did not improve the vitrification outcome where the viability was 4-fold lower than that of freezing. The functionality of cryopreserved cells was tested by interspecific transplantation into sterilized goldfish recipients. The exogenous origin of the germ cells in gonads of goldfish recipient was confirmed by molecular markers and incorporation rate was over 40% at 3 months post-transplantation. Results of this study can serve for long-term preservation of germplasm in carp which can be recovered in a surrogate recipient.
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- 2019
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8. PRESENCE OF THE MOST ECONOMICALLY IMPORTANT FISH SPECIES IN THE RIVER DANUBE AND ITS FLOOD ZONES IN SERBIA
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Zoran Marinović, Nikolina Novakov, Dragana Ljubojević, Miroslav Ćirković, Ester Popović, Desanka Kostić, and Jelena Lujić
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economically important fish species ,the river danube ,flood zones ,threatened fish species ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
On the basis of the data published between 1983 and 2008, this paper shows presence of tench (Tinca tinca), carp (Cyprinus carpio), pike (Esox lucius), catfish (Silurus glanis), zander (Sander lucioperca) and sterlet (Acipenser ruthenus) in the Gornje Podunavlje and Koviljsko-Petrovaradinski Rit Special Nature Reserves and the Begečka Jama Nature Park, as well as in the part of the Danube’s bed between river kilometre 1249- and 1255. The number of these fish species in the River Danube and its flood zones in this part of the Pannonian Basin is decreasing due to the use of inappropriate “tools”, electric current, uncontrolled agricultural and industrial waste dumps, degradation of the habitat. The above mentioned species deserve attention because, according to the Rulebook on declaration and protection of protected and strictly protected wild species of plants, animals and fungi of 2010, in Serbia, T. tinca is a strictly protected species, while C. carpio, E. lucius, S. glanis, S. lucioperca and A. ruthenus are listed as protected. On the basis of the 2009 Fisheries Law in Serbia, permanently closed fishing season was introduced for tench, and closed season in certain periods and fishing ban for individuals under the prescribed size for the rest of the above mentioned species. A. ruthenus is subject to the Law on Ratification of Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), ANNEX II and is listed in the category of species that may be in danger of extinction. Furthermore, it can be found on the IUCN Red List of Endangered Species of the International Union for Conservation of Nature – VU, which means that it is marked as vulnerable.
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- 2012
9. The effects of cryopreservation and cold storage on sperm subpopulation structure of common carp (Cyprinus carpio L.)
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Jelena Lujić, Zoran Marinović, Ilija Šćekić, Ákos Horváth, and Béla Urbányi
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Male ,endocrine system ,Carps ,Motility ,Cold storage ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Cyprinus ,law.invention ,Andrology ,03 medical and health sciences ,Common carp ,0302 clinical medicine ,law ,Animals ,Humans ,030219 obstetrics & reproductive medicine ,biology ,urogenital system ,Chemistry ,Extender ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Spermatozoa ,040201 dairy & animal science ,Sperm ,Sperm Motility ,General Agricultural and Biological Sciences ,Spermatozoa motility ,Semen Preservation - Abstract
The objectives of this study were to identify the presence of different spermatozoa subpopulations (SPs) according to their kinematic characteristics in the sperm of common carp and to test the effects of cryopreservation and prolonged (6-day) storage at room temperature (RT; 23 °C) and 4 °C on spermatozoa motility and subsequently on SP dynamics. Two-step clustering analyses identified three motile SPs based on their kinematic properties: SP1 contained spermatozoa with low velocity and low/moderate STR/LIN values (slow non-linear SP); SP2 was comprised of spermatozoa with high velocities and high STR/LIN values (fast linear SP); SP3 was characterized with high VCL, and moderate LIN/STR (fast non-linear SP); and an additional SP0 was added comprising immotile spermatozoa. Total motility, progressive motility and VCL decreased after cryopreservation to approximately 50% of their value in fresh sperm, while the frequency of SPs characterized by high values of motility parameters declined in favor of those with low motility values and SP0. Motility values of fresh and cryopreserved spermatozoa which were washed with fresh extender after thawing decreased significantly after 24 h of storage at RT and after 72 h of storage at 4 °C, while cryopreserved sperm which remained in the original cryomedium faced a steep decline in motility after only 2 h of storage. As subpopulation frequencies followed this dynamic, this indicates that cryopreserved sperm should be washed with fresh extender in order to obtain favorable sperm kinematic properties after freezing.
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- 2021
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10. A novel strategy for conservation of European eel (Anguilla anguilla) genetic resources: Cryopreservation of ovarian stem cells
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Tamás Müller, Nevena Kitanović, Béla Urbányi, Zoran Marinović, Ilija Šćekić, Jelena Lujić, and Ákos Horváth
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Cell Survival ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,Cryoprotective Agents ,0302 clinical medicine ,Genetic resources ,Animals ,Dimethyl Sulfoxide ,Vitrification ,030219 obstetrics & reproductive medicine ,Preservation methods ,Dimethyl sulfoxide ,Stem Cells ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,Anguilla ,040201 dairy & animal science ,Transplantation ,chemistry ,Female ,Ovarian cell ,Stem cell ,General Agricultural and Biological Sciences - Abstract
The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was developed by testing different cryomedia and technical aspects of freezing. Utilization of 1.5 M of dimethyl sulfoxide (Me2SO), 0.1 M glucose and 1.5% BSA yielded optimal OSCs survival. Additionally, equilibration of 50-mg ovarian fragments for 30 min and plunging into lN2 at −80 °C displayed the highest OSC viability. Different cooling rates ranging from −1 to −40 °C/min did not significantly affect OSC viability when thawing in a 10 °C water bath. In addition, application of needle-immersed vitrification (NIV), combining ES3 (1.5 M PG and 1.5 M Me2SO) with VS3 (3 M PG and 3 M Me2SO) yielded the highest viability rates. Finally, hypothermic storage (4 °C) of ovarian fragments and ovarian cell suspensions displayed favorable viability of ~90% after 48 h of storage and ~65% after 72 h of storage. The development of OSC preservation methods presents an onset of further development of germline stem cell (GSC) manipulation techniques in this species. Cryopreservation of OSCs can enable a continuous supply of cells for either transplantation or in vitro cell culture thus enabling new and improved management and conservation strategies for this endangered species.
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- 2020
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11. Predicting population's oocyte maturation competence and evaluating individual's latency time using in vitro oocyte maturation in pikeperch (Sander lucioperca)
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Uroš Ljubobratović, Nevena Kitanović, Sylvain Milla, Zoran Marinović, Georgina Fazekas, Jelena Stanivuk, Zoltán Nagy, and Ákos Horváth
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Aquatic Science - Published
- 2023
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12. Cyanobacteria, cyanotoxins, and their histopathological effects on fish tissues in Fehérvárcsurgó reservoir, Hungary
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Jussi Meriluoto, Snežana B. Simić, Damjana Drobac Backović, Nevena Kitanović, Zorica Svirčev, Ilija Šćekić, Tamara Dulić, Zoran Marinović, Nada Tokodi, Jelena Lujić, Nevena B. Đorđević, and Béla Urbányi
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Cyanobacteria ,Microcystis ,microcystin ,Microcystins ,Zoology ,Microcystin ,Management, Monitoring, Policy and Law ,Fish histology ,cyanobacterial blooming ,Aphanizomenon ,Freshwater ecosystem ,Article ,chemistry.chemical_compound ,Animals ,Ecosystem ,General Environmental Science ,chemistry.chemical_classification ,Saxitoxin ,Hungary ,biology ,General Medicine ,Cyanotoxin encoding genes ,fish histology ,Plankton ,biology.organism_classification ,Pollution ,Lakes ,cyanotoxin encoding genes ,chemistry ,Carassius ,Environmental Monitoring ,Cyanobacterial blooming - Abstract
Cyanobacteria are important members of lake plankton, but they have the ability to form blooms and produce cyanotoxins and thus cause a number of adverse effects. Freshwater ecosystems around the world have been investigated for the distribution of cyanobacteria and their toxins and the effects they have on the ecosystems. Similar research was performed on the Fehérvárcsurgó reservoir in Hungary during 2018. Cyanobacteria were present and blooming, and the highest abundance was recorded in July (2,822,000 cells/mL). The species present were Aphanizomenon flos-aquae, Microcystis flos-aquae, Microcystis wesenbergii, Cuspidothrix issatschenkoi, Dolichospermum flos-aquae, and Snowella litoralis. In July and September, the microcystin encoding gene mcyE and the saxitoxin encoding gene sxtG were amplified in the biomass samples. While a low concentration of microcystin-RR was found in one water sample from July, analyses of Abramis brama and Carassius gibelio caught from the reservoir did not show the presence of the investigated microcystins in the fish tissue. However, several histopathological changes, predominantly in gills and kidneys, were observed in the fish, and the damage was more severe during May and especially July, which coincides with the increase in cyanobacterial biomass during the summer months. Cyanobacteria may thus have adverse effects in this ecosystem.
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- 2021
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13. Cryopreservation of Danube barbel Barbus balcanicus sperm and its effects on sperm subpopulation structure
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M Natasa Kojadinovic, Z Tijana Velickovic, M Vladica Simic, M Marija Jakovljevic, Ákos Horváth, Zoran Marinović, and M Aleksandra Miloskovic
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Cryoprotectant ,Motility ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,fish conservation ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,spermatozoa ,lcsh:QH301-705.5 ,030304 developmental biology ,balkan barbel ,0303 health sciences ,Barbel ,biology ,Dimethyl sulfoxide ,04 agricultural and veterinary sciences ,Barbus balcanicus ,Liquid nitrogen ,biology.organism_classification ,Sperm ,sperm subpopulation ,lcsh:Biology (General) ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,General Agricultural and Biological Sciences - Abstract
The objective of this study was to develop a successful freezing protocol for cryopreservation of Danube barbel sperm, and to identify the presence of different spermatozoa subpopulations. By testing different concentrations of different cryoprotectants, we determined that the use of 5% dimethyl sulfoxide (DMSO) yielded the highest total motility of ~25%. Cooling rates influenced by frame height and cooling time in liquid nitrogen vapor showed that a frame height of 3 cm and cooling time of 2 min yielded the highest post-thaw motility. Supplementation of cryomedia with 0.1 M of sugars led to an increase in the total post-thaw motility by ~50%, while protein supplementation lowered post-thaw motility. Motile spermatozoa hierarchically clustered according to their motility parameters, displaying a four-subpopulation (SP1-SP4) structure. SP1 was defined by low values of velocity but high overall linearity; SP2 was comprised of fast non-linear spermatozoa, that had high velocity values but low linearity; SP3 was characterized by fast linear spermatozoa, and SP4 by slow non-linear spermatozoa. Protocols developed in this study will lead to the creation of new and enhanced conservation strategies for this species.
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- 2020
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14. Preservation of female genetic resources of common carp through oogonial stem cell manipulation
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Tomáš Tichopád, Zoran Marinović, Xuan Xie, Jelena Lujić, Ákos Horváth, Roman Franěk, Christoph Steinbach, Vojtěch Kašpar, and Martin Pšenička
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Sucrose ,Carps ,Cryoprotectant ,Cell Survival ,Oogonial Stem Cells ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,Common carp ,Cryoprotective Agents ,Oogonia ,0302 clinical medicine ,Freezing ,Animals ,Dimethyl Sulfoxide ,Carp ,030219 obstetrics & reproductive medicine ,biology ,Dimethyl sulfoxide ,Methanol ,Ovary ,0402 animal and dairy science ,Trehalose ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Propylene Glycol ,040201 dairy & animal science ,Transplantation ,chemistry ,Female ,General Agricultural and Biological Sciences - Abstract
Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (−1 °C/min) and short-term storage (−80 or 4 °C) of common carp ovarian tissue fragments with an emphasis on oogonial stem cells (OSCs). Dimethyl sulfoxide (Me2SO) with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. When comparing supplementation of sugars (glucose, trehalose, sucrose) in different concentrations (0.1, 0.3, 0.5 M), glucose and trehalose in 0.3 M were identified as optimal. Short-term storage options for ovarian tissue pieces at −80 °C and 4 °C were tested as alternatives to cryopreservation and storage in liquid nitrogen. The presence of OSCs was confirmed by immunocytochemistry and viability after storage was determined by the trypan blue exclusion test. This study identified the optimal protocol for OSC cryopreservation using slow rate freezing resulting in ∼65% viability. The frozen/thawed OSCs were labelled by PKH-26 and transplanted into goldfish recipients. The success of the transplantation was confirmed by presence of fluorescent cells in the recipient gonad and later on by RT-PCR with carp dnd1 specific primers. The results of this study can facilitate long-term preservation of common carp germplasm which can be recovered in a surrogate recipient through interspecific germ cell transplantation.
- Published
- 2019
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15. Development of sperm vitrification protocols for two endangered salmonid species: the Adriatic grayling, Thymallus thymallus, and the marble trout, Salmo marmoratus
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Dusan Jesensek, Béla Urbányi, Tímea Kollár, Jelena Lujić, Eszter Kása, Ákos Horváth, Zoltán Bokor, Kinga Katalin Lefler, Zoran Marinović, and Gergely Bernáth
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Male ,0301 basic medicine ,Cryoprotectant ,Physiology ,Aquatic Science ,Biochemistry ,03 medical and health sciences ,Cryoprotective Agents ,0302 clinical medicine ,Animal science ,Animals ,Vitrification ,Salmo ,Cryopreservation ,030219 obstetrics & reproductive medicine ,biology ,Hatching ,Endangered Species ,Grayling ,General Medicine ,biology.organism_classification ,Thymallus ,Sperm ,Semen Analysis ,Trout ,030104 developmental biology ,Fertilization ,Sperm Motility ,Salmonidae ,Semen Preservation - Abstract
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA). Fertilization trials were carried out to test the effectiveness of vitrification in the case of grayling. A protocol utilizing a glucose-based extender, 30% cryoprotectants (15% methanol + 15% propylene glycol), 1:1 dilution ratio, and droplets of 2 μl on a Cryotop as cooling device yielded the highest post-thaw motility values for both Adriatic grayling (7.5 ± 6.5%) and marble trout (26.6 ± 15.8%). Viable embryos were produced by fertilizing eggs with vitrified grayling sperm (hatching 13.1 ± 11.7%, control hatching 73.9 ± 10.4%). The vitrification protocol developed in this study can be utilized in the conservation efforts for the two species as an alternative to slow-rate freezing when working in field conditions or when specific equipment necessary for slow-rate freezing is not available.
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- 2018
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16. Interspecific germ cell transplantation: a new light in the conservation of valuable Balkan trout genetic resources?
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Jelena Lujić, Simona Sušnik Bajec, Zoran Marinović, Béla Urbányi, Ákos Horváth, and Ida Djurdjevič
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0106 biological sciences ,0301 basic medicine ,Conservation of Natural Resources ,endocrine system ,Embryo, Nonmammalian ,animal structures ,Cell Transplantation ,Physiology ,Transplantation, Heterologous ,Embryonic Development ,Zoology ,Aquatic Science ,Biology ,010603 evolutionary biology ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,Brown trout ,Animals ,Salmo ,urogenital system ,Cell Differentiation ,Grayling ,Balkan Peninsula ,General Medicine ,biology.organism_classification ,Thymallus ,European grayling ,Transplantation ,Trout ,Germ Cells ,030104 developmental biology ,Oncorhynchus mykiss ,Rainbow trout ,Salmonidae - Abstract
Interspecific transplantation of germ cells from the brown trout Salmo trutta m. fario and the European grayling Thymallus thymallus into rainbow trout Oncorhynchus mykiss recipients was carried out in order to improve current practices in conservation of genetic resources of endangered salmonid species in the Balkan Peninsula. Current conservation methods mainly include in situ efforts such as the maintenance of purebred individuals in isolated streams and restocking with purebred fingerlings; however, additional ex situ strategies such as surrogate production are needed. Steps required for transplantation such as isolation of high number of viable germ cells and fluorescent labeling of germ cells which are to be transplanted have been optimized. Isolated and labeled brown trout and grayling germ cells were intraperitoneally transplanted into 3 to 5 days post hatch rainbow trout larvae. Survival of the injected larvae was comparable to the controls. Sixty days after transplantation, fluorescently labeled donor cells were detected within the recipient gonads indicating successful incorporation of germ cells (brown trout spermatogonia and oogonia-27%; grayling spermatogonia-28%; grayling oogonia-23%). PCR amplification of donor mtDNA CR fragments within the recipient gonads additionally corroborated the success of incorporation. Overall, the transplantation method demonstrated in this study presents the first step and a possible onset of the application of the germ cell transplantation technology in conservation and revitalization of genetic resources of endangered and endemic species or populations of salmonid fish and thus give rise to new or improved management strategies for such species.
- Published
- 2018
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17. Corrigendum to 'Preservation of female genetic resources of common carp through oogonial stem cell manipulation' [Cryobiology 87 (2019) 78–86 0011–2240]
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Jelena Lujić, Tomáš Tichopád, Zoran Marinović, Xuan Xie, Roman Franěk, Vojtěch Kašpar, Christoph Steinbach, Ákos Horváth, and Martin Pšenička
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Common carp ,Cryobiology ,Genetic resources ,Zoology ,General Medicine ,Biology ,Stem cell ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Published
- 2020
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18. Preservation of common carp germ cells under hypothermic conditions: Whole tissue vs isolated cells
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Jelena Lujić, Eszter Kása, Béla Urbányi, Zoran Marinović, Ilija Šćekić, and Ákos Horváth
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0301 basic medicine ,Carps ,Time Factors ,Cell Survival ,Cold storage ,Broodstock ,Cell Separation ,Cryopreservation ,Oogonial Stem Cells ,Andrology ,03 medical and health sciences ,Common carp ,0302 clinical medicine ,Endocrinology ,Cryoprotective Agents ,Animals ,Viability assay ,Carp ,030219 obstetrics & reproductive medicine ,biology ,biology.organism_classification ,030104 developmental biology ,Germ Cells ,Animal Science and Zoology ,Stem cell ,Biotechnology - Abstract
The aim of this study was to optimize the conditions for hypothermic storage of spermatogonial stem cells (SSCs) and oogonial stem cells (OSCs) of common carp Cyprinus carpio. This was conducted by storing gonadal tissue or isolated cells for 24 hr under hypothermic conditions in the first experiment and by testing two different storage media (L-15 or DMEM supplemented with 10% FBS and 25 mM HEPES) and regular medium change (every 4 days) during two weeks of hypothermic storage in the second experiment. During the first 24 hr, isolated cells showed no decrease in viability, while cells obtained from hypothermically stored tissues displayed significantly lower viability after only 6 hr (Tukey's HSD, p
- Published
- 2018
19. Comparative study of growth in Carassius gibelio (Bloch, 1782) and Rutilus rutilus (L., 1758) from two Serbian reservoirs: Multi-model analysis and inferences
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Goran Marković, Violeta Bolić-Trivunović, Jelena Lujić, and Zoran Marinović
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0106 biological sciences ,Prussian carp ,biology ,Ecology ,Water flow ,010604 marine biology & hydrobiology ,Gompertz function ,04 agricultural and veterinary sciences ,Aquatic Science ,biology.organism_classification ,Von bertalanffy ,01 natural sciences ,Predation ,040102 fisheries ,Carassius ,0401 agriculture, forestry, and fisheries ,Akaike information criterion ,Rutilus - Abstract
We assessed growth patterns of Prussian carp Carassius gibelio (Bloch, 1782) and roach Rutilus rutilus (L., 1758) from two different types of reservoirs (lowland type vs highland type) through multi-model analysis. We applied three different fish growth models: von Bertalanffy (VBGF), Gompertz (GGF) and Robertson (RGF) growth functions and assessed the best fitting model using the Akaike's Information Criterion (AIC). Additionally, we calculated deviations from the best fitting model ( Δ i ) and Akaike weights ( w i ) for all models. The VBGF was not the best fitting model in all cases. Furthermore, in many cases more than one model was significantly supported ( Δ i w i F -statistic displayed significant differences in the best fitting growth functions between the Gruža and Međuvrsje Reservoirs for both species. As hypothesized, individuals inhabiting the calm, shallow lowland reservoir with a large surface area attained higher asymptotic lengths than individuals inhabiting narrow highland reservoir with high water flow. Fish inhabiting narrow water bodies with high water flow need to adapt specifically to these conditions since water current affects the movement of fish as well as prey encounter rate, thus smaller bodies which allow higher maneuverability appear to be favored when compared to bulkier body forms in such environments. Therefore, we assume that differences in growth patterns observed in this study predominantly present a response to the highly differential environmental conditions from the two reservoirs.
- Published
- 2016
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20. Fertilizing capacity and motility of tenchTinca tinca(L., 1758) sperm following cryopreservation
- Author
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Gergely Bernáth, Béla Urbányi, Jelena Lujić, Vladica Simić, Zoran Marinović, Miroslav Ćirković, Nataša Radojković, and Ákos Horváth
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0301 basic medicine ,Cryoprotectant ,Hatching ,Extender ,Motility ,04 agricultural and veterinary sciences ,Anatomy ,Aquatic Science ,Biology ,Sperm ,Cryopreservation ,law.invention ,03 medical and health sciences ,030104 developmental biology ,Human fertilization ,Animal science ,law ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Sperm motility - Abstract
Experiments were carried out to develop an optimal cryopreservation protocol for tench sperm by testing the fertilizing capacity and motility parameters including progressive motility, curvilinear velocity (VCL) and linearity (LIN) of cryopreserved sperm. Three experiments were designed to this aim: first experiment where we tested the effects of two extenders (sugar-based Grayling and ion-based Kurokura 180) and two cryoprotectants (DMSO and methanol) on fertilization and hatching success; second where we tested the effect of cryoprotectant type (methanol or DMSO) in different concentrations (5%, 10% and 15%) on fertilization and hatching success; and third where we tested the effect of two cryoprotectants (methanol and DMSO) on sperm motility parameters (progressive motility, VCL and LIN) after 4 h post-thaw storage (4°C). Sperm prepared with the sugar-based Grayling extender displayed better fertilization and hatching rates independently of the applied cryoprotectant most likely due to glucose present which acted as an external cryoprotectant. Concerning cryoprotectant concentrations, the use of 10% methanol yielded the highest fertilization (85 ± 15%) and hatching (80 ± 13%) rates, which were significantly higher than in all other groups. During the post-thaw storage time, 5% methanol, 10% methanol and 5% DMSO groups had significantly higher motility parameters than other groups and we observed no significant decline in any of the parameters during the storage time. Overall, we found that a sugar-based extender in combination with methanol as cryoprotectant is suitable for the cryopreservation of tench sperm and allows storage of cryopreserved sperm for up to 4 h post thaw.
- Published
- 2015
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21. Toxicopathology Induced by Microcystins and Nodularin: A Histopathological Review
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Bratislav Stojiljković, Jelena Lujić, Damjana Drobac, Jussi Meriluoto, Zoran Marinović, Nada Tokodi, and Zorica Svirčev
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Cyanobacteria ,Cancer Research ,Microcystins ,biology ,Carcinogenesis ,Health, Toxicology and Mutagenesis ,Physiology ,biology.organism_classification ,Peptides, Cyclic ,Nodularin ,Acute toxicity ,Fish Diseases ,chemistry.chemical_compound ,chemistry ,Environmental chemistry ,polycyclic compounds ,Animals ,%22">Fish ,Tumor promotion - Abstract
Cyanobacteria are present in all aquatic ecosystems throughout the world. They are able to produce toxic secondary metabolites, and microcystins are those most frequently found. Research has displayed a negative influence of microcystins and closely related nodularin on fish, and various histopathological alterations have been observed in many organs of the exposed fish. The aim of this article is to summarize the present knowledge of the impact of microcystins and nodularin on the histology of fish. The observed negative effects of cyanotoxins indicate that cyanobacteria and their toxins are a relevant medical (due to irritation, acute poisoning, tumor promotion, and carcinogenesis), ecotoxicological, and economic problem that may affect both fish and fish consumers including humans.
- Published
- 2015
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22. CSONTOSHALAK ŐSIVARSEJTJEINEK, SPERMATOGÓNIUMAINAK ÉS OOGÓNIUMAINAK MÉLYHŰTÉSE ÉS ÁTÜLTETÉSE.
- Author
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ÁKOS, HORVÁTH, ZORAN, MARINOVIĆ, JELENA, LUJIĆ, NEVENA, KITANOVIĆ, ILIJA, ŠĆEKIĆ, GYÖRGY, HOITSY, and BÉLA, URBÁNYI
- Abstract
Copyright of Animal Breeding & Feeding / Állattenyésztés és Takarmányozás is the property of Herman Otto Intezet Nonprofit Kft. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
23. Slow-rate freezing of tench and goldfish testicular cells and tissue
- Author
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Gergely Bernáth, Béla Urbányi, Jelena Lujić, Zoran Marinović, Eszter Kása, and Ákos Horváth
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Andrology ,Chemistry ,Slow rate ,General Medicine ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Published
- 2016
- Full Text
- View/download PDF
24. Vitrification of fish sperm: Investigation of the supposed positive effect of trehalose
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I. Di Chiacchio, Tímea Kollár, Jelena Lujić, Gergely Bernáth, Zoran Marinović, Ákos Horváth, Béla Urbányi, and Eszter Kása
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030219 obstetrics & reproductive medicine ,General Medicine ,Anatomy ,Biology ,Sperm ,Trehalose ,General Biochemistry, Genetics and Molecular Biology ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,chemistry ,%22">Fish ,Vitrification ,030212 general & internal medicine ,General Agricultural and Biological Sciences - Published
- 2016
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25. Slow-rate freezing of brown trout gonadal tissue for improved population management
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Eszter Kása, Béla Urbányi, S. Susnik, Ida Djurdjevič, Zoran Marinović, Jelena Lujić, Aleš Snoj, and Ákos Horváth
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Fishery ,Brown trout ,Slow rate ,Zoology ,Population management ,General Medicine ,Biology ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Published
- 2016
- Full Text
- View/download PDF
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