1. Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
- Author
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Rabilloud, Thierry, Biochimie et biophysique des systèmes intégrés (BBSI), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Biochimie et biophysique des systèmes intégrés ( BBSI ), and Université Joseph Fourier - Grenoble 1 ( UJF ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS )
- Subjects
Erythrocytes ,MESH : Isoelectric Focusing ,Detergents ,Chemical Fractionation ,MESH: Thiourea ,MESH: Chemical Fractionation ,MESH : Urea ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Humans ,Urea ,Quantitative Biology - Genomics ,MESH : Thiourea ,Electrophoresis, Gel, Two-Dimensional ,MESH: Urea ,Genomics (q-bio.GN) ,MESH: Hydrophobicity ,MESH: Humans ,MESH : Chemical Fractionation ,MESH : Solubility ,MESH: Erythrocytes ,MESH : Humans ,Thiourea ,Membrane Proteins ,MESH : Detergents ,MESH : Electrophoresis, Gel, Two-Dimensional ,MESH: Electrophoresis, Gel, Two-Dimensional ,MESH: Solubility ,Solubility ,MESH : Membrane Proteins ,[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,FOS: Biological sciences ,MESH: Isoelectric Focusing ,MESH : Hydrophobicity ,MESH: Membrane Proteins ,Isoelectric Focusing ,Hydrophobic and Hydrophilic Interactions ,MESH : Erythrocytes ,MESH: Detergents - Abstract
International audience; Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins, including hydrophobic and membrane proteins. Unfortunately, poor solubility hampers the analysis of these molecules. As these problems arise mainly in the extraction and isoelectric focusing steps, the solution is to improve protein solubility under the conditions prevailing during isoelectric focusing. This chapter describes the use of chaotropes and novel detergents to enhance protein solubility during sample extraction and isoelectric focussing, and discusses the contribution of these compounds to improving proteomic analysis of membrane proteins.
- Published
- 2009