1. Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase
- Author
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Prem C. Pandey, Canh Tran-Minh, Satish Kumaran, Laboratoire de Biotechnologie (LB-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Department of Applied Chemistry (DAC), Banaras Hindu University [Varanasi] (BHU), and Banaras Hindu University
- Subjects
Nicotine ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Immobilized enzyme ,enzyme electrode ,Biomedical Engineering ,Biophysics ,Enzyme electrode ,Biosensing Techniques ,Methyl Parathion ,Paraoxon ,Fluorides ,chemistry.chemical_compound ,Electrochemistry ,medicine ,Organic chemistry ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,[INFO.INFO-BT]Computer Science [cs]/Biotechnology ,enzyme inhibition ,Cholinesterase ,Chromatography ,biology ,Chemistry ,toxic detection ,General Medicine ,Acetylcholinesterase ,Acetylcholine ,potentiometry ,Enzyme inhibitor ,Malathion ,biology.protein ,Cholinesterase Inhibitors ,Fluoride ,Biotechnology ,medicine.drug - Abstract
International audience; Acetylcholine esterase electrodes, based on glass, Pd/PdO and Ir/IrO2 electrodes as pH sensor, using the immobilized acetylcholine esterase in acrylamide-methacrylamide hydrazides prepolymer are reported and compared. New data on the analysis of nicotine, fluoride ion, and some organophosphorus compounds are reported using the present AChE sensor based on the inhibition of the immobilized acetylcholine esterase. Reactivation of immobilized AChE after inhibition with reversible inhibitor, i.e. nicotine and fluoride ion is carried out using a mixture of working buffer and acetylcholine, whereas reactivation after inhibition with irreversible inhibitor, i.e. organophosphorus compounds is carried out using a mixture of acetylcholine and pyridine-2-aldoxime methiodide (PAM). The detection limits for the nicotine and fluoride ion are found to be 10−5M whereas for paraoxon, methyl parathion and malathion are found to be 10−9 M and 10−10 M.
- Published
- 1990
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