1. Optical Diffraction Tomography Meets Fluorescence Localization Microscopy
- Author
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Pham, Thanh-An, Soubies, Emmanuel, Soulez, Ferréol, Unser, Michael, Massachusetts Institute of Technology (MIT), Signal et Communications (IRIT-SC), Institut de recherche en informatique de Toulouse (IRIT), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Toulouse Mind & Brain Institut (TMBI), Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Centre de Recherche Astrophysique de Lyon (CRAL), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Biomedical Imaging Group [Lausanne], Ecole Polytechnique Fédérale de Lausanne (EPFL), ANR-11-LABX-0040,CIMI,Centre International de Mathématiques et d'Informatique (de Toulouse)(2011), and ANR-21-CE48-0008,MICROBLIND,Problèmes inverses aveugles et microscopie optique(2021)
- Subjects
Inverse problems ,Signal Processing (eess.SP) ,Localization Microscopy ,[INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing ,[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging ,FOS: Electrical engineering, electronic engineering, information engineering ,FOS: Physical sciences ,Electrical Engineering and Systems Science - Signal Processing ,Diffraction Tomography ,[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing ,Physics - Optics ,Optics (physics.optics) - Abstract
We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the illumination sources are fluorophores within the sample. Building upon this model, we propose a joint optimization framework to estimate both the refractive index map and the position of fluorescent molecules from the sole SMLM frames., Comment: Presented in ISCS23
- Published
- 2023
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