1. In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea
- Author
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de Hoon, Inès, Boukherroub, Rabah, de Smedt, Stefaan, Szunerits, Sabine, Sauvage, Félix, Universiteit Gent = Ghent University (UGENT), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Financial support from the 'Programme Investissement d’Avenir' (I-SITE ULNE/ANR-16-IDEX-0004 ULNE) managed by the 'Agence Nationale de la Recherche' is acknowledged. In addition, this project has received support from the European Union’s Horizon 2020 research and innovation program under the 'Marie Sklodowska-Curie grant' (agreement no. 847568). Financial support from the Centre National de la Recherche Scientifique (CNRS) and the University of Lille are also acknowledged. This work was partly supported by the French Renatech network., Renatech Network, ANR-16-IDEX-0004,ULNE,ULNE(2016), and European Project: 847568,H2020,H2020-MSCA-COFUND-2018,PEARL(2019)
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[PHYS]Physics [physics] ,corneal penetration ,[SPI]Engineering Sciences [physics] ,cornea ,in vitro model ,drug delivery ,drug diffusion ,ex vivo model - Abstract
International audience; Drug permeation across the cornea remains a major challenge due to its unique and complex anatomy and physiology. Static barriers such as the different layers of the cornea, as well as dynamic aspects such as the constant renewal of the tear film and the presence of the mucin layer together with efflux pumps, all present unique challenges for effective ophthalmic drug delivery. To overcome some of the current ophthalmic drug limitations, the identification and testing of novel drug formulations such as liposomes, nanoemulsions, and nanoparticles began to be considered and widely explored. In the early stages of corneal drug development reliable in vitro and ex vivo alternatives, are required, to be in line with the principles of the 3Rs (Replacement, Reduction, and Refinement), with such methods being in addition faster and more ethical alternatives to in vivo studies. The ocular field remains limited to a handful of predictive models for ophthalmic drug permeation. In vitro cell culture models are increasingly used when it comes to transcorneal permeation studies. Ex vivo models using excised animal tissue such as porcine eyes are the model of choice to study corneal permeation and promising advancements have been reported over the years. Interspecies characteristics must be considered in detail when using such models. This review updates the current knowledge about in vitro and ex vivo corneal permeability models and evaluates their advantages and limitations.
- Published
- 2023
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