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International audience; In rubber tree, starch reserves are necessary for growth and latex regeneration when the demand exceeds supply from photosynthesis. It tends to accumulate in the wood near the tapping cut [1,2] whereas sucrose remains rather stable in the wood and decreases in the latex vessels where it is used to regenerate the exported latex [3]. Thus higher starch storage and mobilization ability could sustain higher latex yield. However the enzymatic processes driving the dynamics of starch synthesis and hydrolysis as related to tapping are not known. The objective of the study is to analyze the effects of tapping on the enzymes involved in starch and sucrose metabolism in the wood of rubber trees. The first approach of this study was to set up the most adapted methodology on measurement of total nonstructural carbohydrates (NSC) and related enzymes activities. The experiment was conducted in Hevea brasiliensis (rubber tree), clone RRIM600. Treatments include untapped trees (Control) and yielding trees tapped with Ethephon stimulation (ET). Each treatment includes 6 trees. Samples have been collected along the trunk and separated into 2 parts, wood and bark, from both sides of the trees tapped and untapped panel. The activity of acid invertase (AI), amylase (AMY), sucrose phosphate synthase (SPS) and sucrose synthase (SuSy) were assessed in the soft bark with a method of sample preparation improved for protein recovery and concentration by acetone precipitation. The method yielded higher enzyme activities of SuSy and SPS. In addition, the substrates and product of starch metabolism, i.e. starch, sucrose, fructose and glucose concentrations have been enzymatically measured. The results showed that starch was the major component in wood and sucrose was mostly found in bark. There was no difference between the former drainage area and resting area after 2 untapped years before restarting tapping.

International audience; Rubber Flux aims at providing a complete picture of CO2, water and energy budget of a rubber plantation in Eastern Thailand. Beyond the evaluation of the fluxes, our purpose is to partition them among the different components of the plantation ecosystem (canopy, trunks, roots, under storey, soil) and the different functions (photosynthesis, respiration. evapotranspiration ) in order to understand the factors controlling the carbon, water and energy budgets of the ecosystem. The experiment is situated at the Chachoengsao Rubber Research Station located in Phanom Sarakham district, about 140 km east of Bangkok The observation site is located in a 6 ha plot at the center of the 350 ha station. The plot itself is planted with a monoclonal stand (clone RRIM 600). Trees were 13 years old in 2007. The average height was 20 m and average girth at 1.7 in was 60 cm. Initial planting density was 500 trees/ha and actual stand density was 454 trees/ha in June 2006. Trees are tapped for latex production for 5 years. Carbon fluxes of rubber plantation ecosystem are continuously measured by the eddy covariance method (ED). Evapo-transpiration (ET) is measured by ED and water balance together. Meanwhile, amounts of carbon (C) stored in the trees will be evaluated by measuring biomass increment of the plantation, in combination with estimations of the carbon content at the different compartments. ED methodology was adapted from a similar experiment developed by our research group on another tropical tree crop plantation, coconut tree, in Vanuatu islands. The flux tower is 25 in high. Three-demensional (3-D) sonic anemometer Young 81000V 20 Hz is used together with alt open path gas analyser (LI-7500; LI-COR, Inc., Lincoln, Nebraska, USA). Raw data are collected and pre-processed by the "Tourbillon" software (INRA-Bioclimatologie, Bordeaux, France) for a time-integration period of 300 s. Raw data are post-processed using EdiRe software (University of Edinburgh, UK) into half-hourly values. All data are despiked according to variance filters, planar fit is applied (parameters are calculated monthly), and vapour is corrected for buoyancy. Weather station measures semi-hourly net radiation (Rn), photosynthetically active radiation (PAR), diffuse PAR (PARdiff), reflected PAR (PARreflected), global radiation (Rg), Air temperature (Ta), Relative humidity (Rh), wind speed, wind direction, rainfall, vertical profile of air temperature (TCs). Energy balance will be assessed by measurements of net radiation (Rn) and estimation of the energy partitioning among heat fluxes and heat storage. Results obtained at the ecosystem scale by these methods will be compared to gas exchanges measured at the level of the different compartments (canopy, trunk, root system, soil, etc). Thereby, the validated CO2 and H2O fluxes will be used to model gas exchanges of rubber plantation ecosystem according to climate and other environmental parameters as well as crop management.

International audience; In Thailand, rubber is the major tree crop covering more than 2 millions hectares. Its impact on environment, and particularly CO2 balance and water use, is a major issue. A complete flux-measuring system has been installed in a 13 years old tubber plantation (clone RRIM 600) in eastern Thailand (Chachoengsao Rubber Research Center, RRIT-DOA) in 2006. Carbon fluxes of rubber plantation ecosystem are continuously measured by eddy-covariance method (CO2 exchanges between the ecosystem and the atmosphere). Evapo-transpiration (ET) are measured by eddy-covariance and water balance together and partitioned between tree transpiration and soil evaporation. Sapflow is measured by heat dissipation method (Granier 1985, 1987) using home-made 20 mm-long radial probes, continuously heated (0.2 W). Meanwhile, amounts of C stored in the trees are evaluated by measuring biomass increment along the life cycle of the plantation, in combination with estimations of the carbon content of the different compartments. These measurements will provide the annual balance of C within plantations at different ages. Results obtained at ecosystem scale by these methods will be compared to gas exchanges measured at the level of the different compartments (canopy, trunk, root system, soil...). Particularly, Farquhar's photosynthesis model (Farquhar et al. 1980) is parameterized across the canopy. Leaf water potential, sapflow and canopy transpiration are computed to calculate canopy conductivity. Thereby, validated CO2 and H2O fluxes will be used to model gas exchanges of rubber plantation ecosystem according to climate and other environmental parameters as well as crop management.