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28 results on '"Denis, Isabelle"'

1. Estrogènes et qualité de la semence chez le verrat

Author

Travert, Carine, Galeraud-Denis, Isabelle, Goux, Didier, Ferchaud, Stéphane, Guillouet, Philippe, Magistrini, Michèle, Carreau, Serge, Unité sous contrat aromatase et oestrogènes dans les gonades des mammifères, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], VERRAT, MICROSCOPIE CONFOCALE, [SDV]Life Sciences [q-bio], AROMATASE, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, OESTROGENE, SPERMATOZOIDE
Abstract

National audience

Published
2010

2. Transcripts of aromatase and estrogen receptors and significance of other RNAs in human spermatozoa

Author

Galeraud-Denis Isabelle, Serge Carreau, Unité sous contrat aromatase et oestrogènes dans les gonades des mammifères, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
Male, endocrine system, [SDV]Life Sciences [q-bio], Population, Acrosome reaction, AROMATASE, [INFO] Computer Science [cs], Andrology, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Capacitation, medicine, Humans, FERTILITY, [INFO]Computer Science [cs], RNA, Messenger, Aromatase, SPERMATOZOA, education, reproductive and urinary physiology, 030304 developmental biology, 0303 health sciences, education.field_of_study, 030219 obstetrics & reproductive medicine, biology, urogenital system, Sperm, Protamine, RNAs, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Receptors, Estrogen, MAN, biology.protein, Gamete, Spermatogenesis
Abstract

The existence of a complex population of mRNAs in human sperm is well documented but their role is not completely elucidated. Evidence for a latent transcriptional capacity and/or a potential translation de novo in mature spermatozoa from fertile men has been provided and is helpful in understanding the final steps of sperm maturation (capacitation and/or the acrosome reaction). Spermatogenesis is controlled by gonadotrophins and testosterone, their effects are modulated by locally-produced factors that include estrogens derived from the irreversible transformation of androgens by aromatase. The data demonstrating an additional source of estrogens in rat germ cells along with several studies showing a decreased sperm motility in men deficient in aromatase has led to the further explanation of the expression of aromatase in ejaculated spermatozoa from fertile men. A significant decrease in the amount of aromatase transcripts in the immotile sperm fraction was recorded. In addition, the levels of transcripts encoding for proteins involved in either nuclear condensation protamines 1 and 2 (Prm1 and Prm2) or in capacitation endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide sythase (nNOS) and c-myc were compared in low and high motile sperm prepared from the same sample. No significant change in the ratio of c-myc/Prm2 between the two populations of spermatozoa was observed. Conversely the amount of Prm1 mRNA was significantly higher in the low motile fraction than in the high motile fraction; in most of the high motile sperm samples analyzed, eNOS and nNOS transcripts were undetectable, whereas they were observed in low motile sperm. Moreover, a partial or complete disappearance of c-myc transcripts was observed after capacitation. Analysis of the mRNA profile in human ejaculated sperm could be helpful either as a diagnostic tool to evaluate the male gamete quality and/or as a prognostic value for fertilization and embryo development.

Published
2007

3. Astrocytes in culture need docosahexaenoic acid (DHA) to restore the n-3/n-6 polyunsaturated fatty acid (PUFA) balance in their membrane

Author

Champeil-Potokar, Gaëlle, Denis, Isabelle, Langelier, Bénédicte, Alessandri, Jean-Marc, Guesnet, Philippe, Lavialle, Monique, and Champeil-Potokar, Gaëlle

Subjects
[SDV] Life Sciences [q-bio], cell culture, astrocytes, arachidonic acid, membrane phospholipids, docosahexaenoic acid
Abstract

Docosahexaenoic acid (DHA), the main n-3 polyunsaturated fatty acid (PUFA) in membranes, is particularly abundant in brain cells. Decreased cerebral concentrations of DHA, resulting from dietary n-3 deficiency, are associated with impaired cognitive function. Because the cellular causes of this impairment are still unknown, we need in vitro models that mimic the variations in n-3/n-6 PUFA seen in vivo. We have compared the PUFA profiles of hamster astrocytes cultured in medium supplemented with long-chain PUFA [DHA and/or arachidonic acid (AA)] with those of brain tissue from hamsters fed an n-6/n-3 PUFA-balanced diet or one lacking n-3 PUFA. Astrocytes were obtained from the brain cortex of newborn hamsters and cultured in minimum essential medium + 5% fetal calf serum (FCS) supplemented with DHA and/or AA for 10 days. The astrocytes cultured in medium + FCS had low n-3 PUFA contents, comparable to those of brain tissue from hamsters fed an n-3-deficient diet. We have shown that astrocytes grown in medium supplemented with DHA and/or AA, plus alpha-tocopherol to prevent lipid peroxidation, incorporated large amounts of these long-chain PUFA, so that the n-6/n-3 PUFA compositions of the phosphatidylethanolamine and phosphatidylcholine, the two main classes of membrane phospholipids, were greatly altered. Astrocytes cultured in medium plus DHA had a more physiological n-3 status, grew better, and retained their astrocyte phenotype. Thus astrocytes in culture are likely to be physiologically relevant only when provided with adequate DHA. This reliable method of altering membrane phospholipid composition promises to be useful for studying the influence of n-6/n-3 imbalance on astrocyte function.

Published
2004

4. Effets d'une variation des apports alimentaires en acides gras poly-insaturés de la série N-3 sur l'horloge circadienne chez le hamster : Etude histologique et comportementale

Author

Champeil-Potokar, Gaëlle, Denis, Isabelle, Papillon, Catherine, Alessandri, Jean Marc, Lavialle, Monique, ProdInra, Migration, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and UR neurobiologie des fonctions végétatives

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], CELLULE NERVEUSE, HISTOLOGIE
Published
2003

5. Effet de la supplémentation en DHA (acide docosahexaéonique) sur la composition lipidique membranaire d'astrocytes en culture

Author

Champeil-Potokar, Gaëlle, Denis, Isabelle, Goustard-Langelier, B., Lavialle, Monique, Alessandri, Jean Marc, ProdInra, Migration, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and UR neurobiologie des fonctions végétatives

Subjects
DHA, [SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], RAT
Published
2001

6. DHA et plasticité astrocytaire dans les noyaux suprachiasmatiques (NSC)

Author

Champeil, Gaelle, Denis, Isabelle, Chaumontet, Catherine, Papillon, Catherine, Lavialle, Monique, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], DHA, [SDV]Life Sciences [q-bio], NEUROBIOLOGIE
Published
2000

10. TGF-beta calcitriol

Author

Heberden, Christine, Denis, Isabelle, Pointillart, Alain, Mercier, Thierry, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], CANCER
Abstract

3 graph.

Published
1998

11. Effets inhibiteurs de la vitamine C sur la formation osseuse in vivo et in vitro chez le porc en croissance

Author

Denis, Isabelle, Zérath, E., Pointillart, Alain, INRA, ., Institut National de la Santé et de la Recherche Médicale, ., ProdInra, Migration, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
1998

19. Increased Ca absorption and bone formation in pigs treated with GH

Author

Pointillart, Alain, Denis, Isabelle, Zérath, E., Thomasset, Monique, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], STH, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1993

22. Effets du traitement par la somatotropine porcine (PST) sur le metabolisme osseux chez le porcelet

Author

Denis, Isabelle, Pointillart, Alain, Zérath, E., Bonneau, M., ProdInra, Migration, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and Laboratoire du jeune ruminant

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], HISTOLOGIE, STH, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
1992

24. Modulation of growth hormone action on porcine osteoblasts by ascorbic acid

Author

Denis, Isabelle, Pointillart, Alain, Lieberherr, Michèle, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], STH
Abstract

Suppl.1

Published
1992

27. Mise en evidence d'un (ou des) inhibiteur(s) de LH dans les serums ovin et humain

Author

Boujrad, N., Denis, Isabelle, Papadopoulos, V., Pisselet, C., Bouchard, Philippe, Drosdowsky, M.A., Hochereau-de-Reviers, M.T., Carreau, Serge, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], LH, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1990

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