1. International study of factors affecting human chromosome translocations
- Author
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Alan Edwards, L. Gayle Littlefield, Nori Nakamura, Günther Stephan, Ursula Oestreicher, E. Janet Tawn, Mimako Nakano, Ernst Schmid, Radim J. Sram, Ruth A. Kleinerman, Firouz Darroudi, Caroline A. Whitehouse, Michael Hauptmann, Alice J. Sigurdson, Vorobtsova Ie, David H. Blakey, James D. Tucker, Joan F. Barquinero, Olena Beskid, Gordon K. Livingston, Laurence Roy, L. Michelle Bennett, P. Voisin, Yoshiaki Kodama, Lee C. Yong, Mina Ha, Isamu Hayata, Manfred Bauchinger, Chunyan Wang, Hai Won Chung, Carita Lindholm, Parveen Bhatti, Wei Zhang, National Cancer Institute [Bethesda] (NCI-NIH), National Institutes of Health [Bethesda] (NIH), Dankook University, Division of Cancer Epidemiology and Genetics, National Institutes of Health [Bethesda] (NIH)-National Institutes of Health [Bethesda] (NIH), Department of Bioinformatics and Statistics, Netherlands Cancer Institute, University of Central Lancashire [Preston] (UCLAN), Radiation and Nuclear Safety Authority [Helsinki] (STUK), Radiation Effects Research Foundation, Central Research Institute of Roentgenology and Radiology [Moscow], Ministry of Health and Social Development of Russian Federation [Moscow], Bundesamt für Strahlenschutz (BfS), National Institute for Occupational Safety and Health [Cincinnati] (NIOSH), Centers for Disease Control and Prevention (CDC), GSF – National Research Centre for Environment and Health, Seoul National University [Seoul] (SNU), Leiden University Medical Centre [Leyde, Pays-Bas], Leiden University, Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Universitat Autònoma de Barcelona (UAB), National Institute of Radiological Sciences (NIRS), National Institute for Radiological Protection (NIRP), Center for Cancer Research, Oak Ridge Associated Universities (ORAU), National Radiological Protection Board (NRPB), and Wayne State University [Detroit]
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Male ,mutation rate ,Health, Toxicology and Mutagenesis ,International Cooperation ,[SDV]Life Sciences [q-bio] ,cigarette smoking ,Chromosomal translocation ,Translocation, Genetic ,030218 nuclear medicine & medical imaging ,0302 clinical medicine ,newborn ,Risk Factors ,Ethnicity ,gender ,80 and over ,Child ,In Situ Hybridization, Fluorescence ,In Situ Hybridization ,Genetics ,Aged, 80 and over ,medicine.diagnostic_test ,adult ,Smoking ,article ,Age Factors ,Middle Aged ,3. Good health ,Europe ,aged ,female ,priority journal ,030220 oncology & carcinogenesis ,Cord blood ,Child, Preschool ,Asia ,Adolescent ,Translocation ,Ethnic Groups ,In situ hybridization ,Biology ,Fluorescence ,Chromosome Painting ,03 medical and health sciences ,Sex Factors ,Genetic ,medicine ,Humans ,controlled study ,human ,Preschool ,fluorescence in situ hybridization ,Chromosome translocations ,Background frequency ,Controls ,Fluorescence in situ hybridization ,aging ,Infant, Newborn ,Chromosome ,Infant ,major clinical study ,Peripheral blood ,confidence interval ,Peripheral blood lymphocyte ,North America ,linear regression analysis ,Poisson distribution ,umbilical cord blood ,chromosome damage ,chromosome translocation ,peripheral lymphocyte - Abstract
Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from 16 laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages versus a linear relationship (p < 0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p < 0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes. © 2008 Elsevier B.V. All rights reserved.
- Published
- 2008
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