1. Use of FTA® card methodology for sampling and molecular characterization of Echinococcus granulosus sensu lato in Africa
- Author
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Ahmed Bezeid El Mamy, Ikhlass El Berbri, Gérald Umhang, Jean-Marc Boucher, Allal Dakkak, Ouafaa Fassi Fihri, Franck Boué, Abdallah Traore, Anne-Françoise Petavy, Vanessa Hormaz, Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Department of Pathology and Veterinary Public Health, National Center for Livestock and for Veterinary Research, Central Veterinary Laboratory, Université Claude Bernard Lyon 1 - Faculté des sciences et technologies (UCBL FST), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, European Project: 221948,EC:FP7:KBBE,FP7-KBBE-2007-2A,ICONZ(2009), and Université Claude Bernard Lyon 1 - Faculté des sciences (UCBL FS) more...
- Subjects
0301 basic medicine ,Veterinary medicine ,[SDV]Life Sciences [q-bio] ,030231 tropical medicine ,Immunology ,Pcr assay ,MESH: Parasitology ,Reference laboratory ,Biology ,MESH: DNA, Helminth ,FTA card ,MESH: Echinococcus granulosus ,Germinal Layer ,03 medical and health sciences ,MESH: Echinococcosis ,0302 clinical medicine ,Sensu ,parasitic diseases ,Cystic Echinococcosis ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,MESH: Specimen Handling ,Echinococcus granulosus ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,Cystic echinococcosis ,Sampling (statistics) ,General Medicine ,030108 mycology & parasitology ,biology.organism_classification ,3. Good health ,Infectious Diseases ,Echinococcus ,Sampling method ,Parasitology ,Molecular diagnosis - Abstract
International audience; Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA® card technology. The DNA samples were taken by applying the FTA® paper on the germinal layer after opening the cysts. The sampling technique was validated using frozen cysts (n = 76) stored in the laboratory and from field samples (n = 134) taken at the slaughterhouse by veterinarian technicians during meat inspection in Morocco, Mali and Mauritania. DNA was extracted after several weeks of storage at room temperature. PCR assays were performed using primers for generic cestode (cox1) and amplified fragments were sequenced. All samples taken in the lab and 80% of field samples were capable of molecular characterization. Cyst-derived DNA from FTA® samples can be useful for easy sampling, storage and rapid, safe and cheap shipment. The use of the FTA methodology will facilitate studies in the field to investigate the presence and genetic characterization of E. granulosus sensu lato in African countries. more...
- Published
- 2017
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